Abstract: The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from Leuconostoc mesenteroides, and subjected to site-directed mutagenesis to improve the enzyme activity of sucrose phosphorylase. The mutant is expressed in Corynebacterium glutamicum and used as a whole cell catalyst to produce 2-O-?-D-glycerol glucoside. At a 5 L fermentation tank level, a large quantity of 2-O-?-D-glycerol glucoside can be produced efficiently in a short time, which is conducive to expanding the prospect of industrial application of sucrose phosphorylase for the production of 2-O-?-D-glycerol glucoside and realizing its large-scale industrial application.

Abstract: Methods for determining a self-driving technology-based resource and communications are provided. One example method includes that a server determines a first time-frequency resource and a first location of the first vehicle; determines a first area range in which the first vehicle is located; when a second vehicle exists in the first area range, and a second time-frequency resource of the second vehicle is the same as the first time-frequency resource, determines, from time-frequency resources in the first area range, a third time-frequency resource including an idle time-frequency resource; and the server sends the third time-frequency resource to the first vehicle, and the first vehicle performs laser signal transmission by using the third time-frequency resource.

Abstract: The disclosure discloses 17?-hydroxysteroid dehydrogenase mutants and application thereof, and belongs to the technical field of biology. The disclosure provides 17?-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y with high specific enzyme activities, and the specific enzyme activities of the 17?-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y are as high as 1.85, 1.93, 2.06 and 5.15 U/mg, respectively, which are 1.11, 1.16, 1.24 and 3.10 times larger than that of wild-type 17?-hydroxysteroid dehydrogenase (1.66 U/mg).

Abstract: The present invention discloses a method for producing a 1,2-amino alcohol compound by utilizing whole-cell transformation, and belongs to the technical field of gene engineering and microorganism engineering. According to the present invention, engineered Escherichia coli co-expresses epoxide hydrolase, alcohol dehydrogenase, ?-transaminase and glutamate dehydrogenase, is capable of realizing whole-cell catalysis of an epoxide in one step to synthesize a 1,2-amino alcohol compound, and meanwhile, can realize regeneration of coenzyme NADP+ and an amino doner L-Glu; alcohol dehydrogenase expressed by the engineered Escherichia coli is RBS optimized alcohol dehydrogenase, and such RBS optimization can control the expression quantity of alcohol dehydrogenase, so that the catalysis rate of alcohol dehydrogenase and transaminase can achieve an optimum ratio, to eliminate influence caused by a rate-limiting step in a catalyzing course.

Abstract: Systems and methods for navigating a user to a destination are provided. An exemplary indoor navigation method uses a terminal device. The method includes capturing, by the terminal device, image data of an indoor scene, and determining a current position of the user based on the captured image data. The method also includes determining a navigation path and a navigation sign for navigating the user from the current position to the destination, and rendering, by the terminal device, a three-dimensional representation of the indoor scene with the navigation sign placed therein based on the image data and the navigation path. The navigation sign signals the navigation path in the three-dimensional representation. The method further includes displaying, on the terminal device, the three-dimensional representation to the user.

Abstract: The present disclosure discloses a method for producing 9?-hydroxy androstane-4-alkene-3,17-diketone by enzymatic conversion, and belongs to the fields of gene engineering and enzyme engineering. According to the present disclosure, oxidation subunit KshA, reduction subunit KshB and unknown active subunit KshC of 3-ketosteroid-9?-hydroxylase sourcing from Mycobacterium sp. Strain VKM Ac-1817D are successfully expressed in E. coli BL21, and KshC is identified as an oxidation subunit, the enzyme activity of which is far higher than that of KshA. BL21/pET-28a(+)-fdh constructed in the laboratory is used for expressing formate dehydrogenase (FDH), and by using crude enzyme liquid of KSH (KshB+KshC) and FDH engineering bacteria as a biocatalyst and a steroidal compound (AD) as a substrate, optimum reaction temperature is determined as 30° C. and optimum pH is determined as 7.0. In optimum conditions, AD is converted to produce a product 9-OH-AD, and within 20 hours, the output of 9-OH-AD is 4.

Abstract: The present disclosure discloses a thermophilic L-asparaginase mutant and screening and fermentation methods thereof, and belongs to the field of gene engineering, enzyme engineering and fermentation engineering. In Bacillus subtilis 168, a Pyrococcus yayanosii CH1-derived L-asparaginase encoding gene is used as a template, and a mutation library is constructed by an error-prone PCR (epPCR) technology. A mutant strain with improved specific enzyme activity is screened through a high-flux screening method of synchronous cell disruption and enzyme activity measurement. Mutated residues included in a positive mutant are analyzed to construct a composite mutant strain S17G/A90S/R156S/K272A with improved specific enzyme activity and specific enzyme activity of 3108 U/mg. An expression quantity of the composite mutant strain in the Bacillus subtilis 168 is increased through measures of a strong promoter P43 and RBS optimization.

Abstract: The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from Leuconostoc mesenteroides, and subjected to site-directed mutagenesis to improve the enzyme activity of sucrose phosphorylase. The mutant is expressed in Corynebacterium glutamicum and used as a whole cell catalyst to produce 2-O-?-D-glycerol glucoside. At a 5L fermentation tank level, a large quantity of 2-O-?-D-glycerol glucoside can be produced efficiently in a short time, which is conducive to expanding the prospect of industrial application of sucrose phosphorylase for the production of 2-O-?-D-glycerol glucoside and realizing its large-scale industrial application.

Abstract: The disclosure discloses 17?-hydroxysteroid dehydrogenase mutants and application thereof, and belongs to the technical field of biology. The disclosure provides 17?-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y with high specific enzyme activities, and the specific enzyme activities of the 17?-hydroxysteroid dehydrogenase mutants V107A, T155N, H164Y and V107A/T155N/H164Y are as high as 1.85, 1.93, 2.06 and 5.15 U/mg, respectively, which are 1.11, 1.16, 1.24 and 3.10 times larger than that of wild-type 17?-hydroxysteroid dehydrogenase (1.66 U/mg).

Abstract: This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

Abstract: The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a ?-amino acid, and providing a practical and effective strategy for industrial production of the ?-amino acid.

Abstract: The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.

Abstract: The present invention discloses a Mycobacterium neoaurum-derived steroid C27-monooxygenase and an application thereof, which belong to the technical fields of genetic engineering and enzyme engineering. By the method of gene knockout and intensive expression, the present invention screens out three isoenzymes of a key enzyme SMO in the process of degrading sterol side chains from Mycobacterium neoaurum. The three isoenzymes are intensively expressed respectively in the Mycobacterium neoaurum for the high yield of androsta-1,4-diene-3,17-dione (ADD), the yield of ADD is increased remarkably, wherein the effect of SMO2 is most remarkable. By overexpressing SMO2, the final ADD yield is increased from 5.2 g·L?1 to 7.3 g·L?1. The present invention provides a helpful guidance for the industrialization of the microbial fermentation method for increasing the ADD yield.

Abstract: A link control node and method, and a system are provided. An MPTCP sublink is identified; and from the MPTCP sublink, a first MPTCP sublink on which link control needs to be performed is determined, and an adjustment manner applicable to the first MPTCP sublink is determined. By adding centralized management and control measures on MPTCP connections, a data transmission process on each MPTCP sublink can be performed on an expected communication link, which adapts to running requirements of a system. Therefore, radio resources can be appropriately allocated, and system performance can be improved.

Abstract: Architecture that decomposes of one or more monolithic data concepts into atomic concepts and related atomic concept dependencies, and provides streaming data processing that processes individual or separate (atomic) data concepts and defined atomic dependencies. The architecture can comprise data-driven data processing that enables the plug-in of new data concepts with minimal effort. Efficient processing of the data concepts is enabled by streaming only required data concepts and corresponding dependencies and enablement of the seamless configuration of data processing between stream processing systems and batch processing systems as a result of data concept decomposition. Incremental and non-incremental metric processing enables realtime access and monitoring of operational parameters and queries.

Abstract: It involves the field of power trading technology and a method for microservice transformation of power trading function comprising the following steps: (1) divide the function in extranet trading cloud into many multiple individual micro services and divide the function in intranet trading cloud into multiple individual micro services; (2) register a node information for each individual micro service in service registry when service deployment starts; (3) extranet users and intranet users enter into extranet trading cloud and intranet trading cloud respectively through PC terminal or mobile terminal, obtain the actual network address that needs to be served from service registry, send a request to the requested service, and perform related business operation.

Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.

Abstract: A connector includes a first pin which is configured to indicate an in-service signal, a second pin which is configured to indicate a power supply signal, a third pin which is configured to indicate a clock signal, and a fourth pin; the first pin which is configured to indicate a PCIe port signal; the first pin, the second pin, the third pin, and the fourth pin have an equal length; and the connector includes a first face and a second face, a limiting structure is arranged on the first face, the limiting structure is a boss or a groove, and the first pin is located in the middle of the first face.

Abstract: A method of detecting a roadway shoulder in an at least partially autonomous vehicle, during a minimum risk maneuver, comprising sensing a vibration within a vehicle, identifying a frequency of the vibration, and determining whether the vehicle is in contact with rumble strips in response to the identified frequency, when at least one or more sensors in a typical known sensor system is compromised.

Abstract: A method and system for navigating traffic for an autonomously controllable vehicle operates by detecting at least one target vehicle within a traffic environment with at least one sensor disposed on a primary vehicle. The system obtains information with a plurality of sensors disposed on the primary vehicle of the traffic environment and the at least target vehicle and predicts a maneuver of the at least one target vehicle with a controller disposed on the primary vehicle for a future time based on the information obtained relating to the traffic environment and the at least one target vehicle. The system further generates a recommended path for the primary vehicle with the controller that avoids conflicts with a future position of the at least one target vehicle based on predicted maneuvers of the at least one target vehicle.