Abstract: The present invention relates to application of a novel signal peptide in L-glutamate and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The signal peptide which mediated secretion of ?-mannanase was invented, and the recombinant strain with this signal peptide had advantages on utilizing konjac powder to produce related products, and its utilization efficiency of konjac powder, production efficiency, and yield were higher than other signal peptides. The recombinant strain possessing this new signal peptide had advantages on utilizing cheaper konjac powder as substrate to lower the process costs on L-glutamic acid and its high-value-added products.

Abstract: The present disclosure discloses a thermophilic L-asparaginase mutant and screening and fermentation methods thereof, and belongs to the field of gene engineering, enzyme engineering and fermentation engineering. In bacillus subtilis 168, a Pyrococcus yayanosii CH1-derived L-asparaginase encoding gene is used as a template, and a mutation library is constructed by an error-prone PCR (epPCR) technology. A mutant strain with improved specific enzyme activity is screened through a high-flux screening method of synchronous cell disruption and enzyme activity measurement. Mutated residues included in a positive mutant are analyzed to construct a composite mutant strain S17G/A90S/R156S/K272A with improved specific enzyme activity and specific enzyme activity of 3108 U/mg. An expression quantity of the composite mutant strain in the bacillus subtilis 168 is increased through measures of a strong promoter P43 and RBS optimization.

Abstract: The present disclosure discloses a method for producing 9?-hydroxy androstane-4-alkene-3,17-diketone by enzymatic conversion, and belongs to the fields of gene engineering and enzyme engineering. According to the present disclosure, oxidation subunit KshA, reduction subunit KshB and unknown active subunit KshC of 3-ketosteroid-9?-hydroxylase sourcing from Mycobacterium sp. Strain VKM Ac-1817D are successfully expressed in E. coli BL21, and KshC is identified as an oxidation subunit, the enzyme activity of which is far higher than that of KshA. BL21/pET-28a(+)-fdh constructed in the laboratory is used for expressing formate dehydrogenase (FDH), and by using crude enzyme liquid of KSH (KshB+KshC) and FDH engineering bacteria as a biocatalyst and a steroidal compound (AD) as a substrate, optimum reaction temperature is determined as 30° C. and optimum pH is determined as 7.0. In optimum conditions, AD is converted to produce a product 9-OH-AD, and within 20 hours, the output of 9-OH-AD is 4.

Abstract: The present invention discloses a single-cell factory for efficiently synthesizing ?-aminobutyric acid and construction and application thereof, which belong to the technical field of microorganisms. The present invention expresses an L-threonine deaminase gene, an L-amino acid dehydrogenase gene and a dehydrogenase gene for providing cofactor NADH cycle in tandem to construct a recombinant Escherichia coli single-cell factory which is used for efficiently synthesizing ?-aminobutyric acid. The expression level of the L-threonine deaminase is optimized and controlled by an RBS sequence, so that the problem of transformation inhibition caused by the rapid accumulation of an intermediate product ketobutyric acid is solved, moreover, the expression level of the dehydrogenase for providing cofactor NADH cycle is optimized and controlled by a promoter and an RBS sequence, consequently, the NADH regeneration rate is increased, and ultimately, yield is increased.

Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.

Abstract: The present invention discloses a Mycobacterium neoaurum-derived steroid C27-monooxygenase and an application thereof, which belong to the technical fields of genetic engineering and enzyme engineering. By the method of gene knockout and intensive expression, the present invention screens out three isoenzymes of a key enzyme SMO in the process of degrading sterol side chains from Mycobacterium neoaurum. The three isoenzymes are intensively expressed respectively in the Mycobacterium neoaurum for the high yield of androsta-1,4-diene-3,17-dione (ADD), the yield of ADD is increased remarkably, wherein the effect of SMO2 is most remarkable. By overexpressing SMO2, the final ADD yield is increased from 5.2 g·L.?1 to 7.3 g·L.?1. The present invention provides a helpful guidance for the industrialization of the microbial fermentation method for increasing the ADD yield.

Abstract: The invention discloses a method for increasing the yield of L-arginine by knocking out flavin reductases, and belongs to the technical field of amino acid production by microbial fermentation. Genes frd1 and frd2 for encoding hypothetic NADPH-dependent FMN reductase in Corynebacterium crenatum SDNN403 are over-expressed in E. coli BL21 and are purified to form target proteins Frd181 and Frd188, and functions of the target proteins are identified to obtain a result showing that the proteins Frd181 and Frd188 both are NAD(P)H-dependent flavin reductases producing H2O2. By taking a genome of the Corynebacterium crenatum SDNN403 as a template, frd1 and frd2 gene deletion fragments are obtained by overlap extension PCR; connecting pK18mobsacB to obtain knockout plasmids pK18mobsacB-?frd1 and pK18mobsacB-?frd2; carrying out electric shock to transform the Corynebacterium crenatum SDNN403; and carrying out secondary screening to obtain recombinant strains 403?frd1 and 403?frd2.

Abstract: This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

Abstract: A water-cooling device includes a liquid reservoir main body having a heat exchange chamber, a pump having a stator and a rotor and a heat exchange component connected with the liquid reservoir main body. The heat exchange chamber is for a cooling liquid to pass through. The rotor is connected with an impeller and exposed to the cooling liquid in the heat exchange chamber. The stator is integrally embedded in the liquid reservoir main body by embedded injection molding. The stator is isolated from the heat exchange chamber. According to the design of the water-cooling device, the structural strength of the liquid reservoir main body is enhanced and a waterproof effect for the stator is achieved.

Abstract: An autonomous driving system for a vehicle includes an autonomous lane change safety check method. An object relative to the vehicle and a current lane of travel is mapped and object data of the object is assessed. An object threat value for the object is assigned based on the object map and the object data. The object is grouped based the object threat value. An overall critical value is determined from the object threat value for the object and filtered to reduce signal noise. The critical value is compared to a predetermined criticality threshold, where the lane change safety check is failed when the critical value is over the threshold.

Abstract: Use of BUBR1 as a biomarker for predicting the response to a compound, preferably resistance of a disease such as cancer in a subject, wherein the compound is a furazanobenzimidazole compound of general formula (I).

Abstract: Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.

Abstract: A method and sensor system are disclosed for automatically determining object sensor position and alignment on a host vehicle. A radar sensor detects objects surrounding the host vehicle in normal operation. Static objects are identified as those objects with ground speed approximately equal to zero. Vehicle dynamics sensors provide vehicle longitudinal and lateral velocity and yaw rate data. Measurement data for the static objects—including azimuth angle, range and range rate relative to the sensor—along with the vehicle dynamics data, are used in a recursive geometric calculation which converges on actual values of the radar sensor's two-dimensional position and azimuth alignment angle on the host vehicle.

Abstract: The present invention relates to application of a novel signal peptide in L-arginine and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The present invention fused the signal peptide set forth in SEQ ID NO.1 with the ?-mannanase of Bacillus subtilis CCTCC M 209200, and expressed the fused gene in the strain with high L-arginine yield. The recombinant strain Corynebacterium crenatum CGMCC 0890/p MSPman had advantages on utilizing cheaper konjac powder as substrate, and after fermenting for 96 hours in a 5 L bioreactor, the L-arginine yield reached 45 g/L. Another two recombinant strains were constructed based on Corynebacterium crenatum CGMCC 0890/pMSPman, and after fermenting for 96 hours in a 5 L bioreactor, the L-ornithine yield and L-citrulline reached 23.5 g/L and 26.3 g/L respectively.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: The present invention relates to application of a novel signal peptide in L-glutamate and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The signal peptide which mediated secretion of ?-mannanase was invented, and the recombinant strain with this signal peptide had advantages on utilizing konjac powder to produce related products, and its utilization efficiency of konjac powder, production efficiency, and yield were higher than other signal peptides. The recombinant strain possessing this new signal peptide had advantages on utilizing cheaper konjac powder as substrate to lower the process costs on L-glutamic acid and its high-value-added products.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: A method of arbitrating conflicting outputs in a redundant control system. Execution data of a task executed by each controller in the redundant control system is recorded. The execution data includes an initial timestamp of each execution stream, identification of critical functions in each execution stream, and parameter values used by the critical functions. A path executed by each controller is identified based only on the critical functions executed for each execution stream. The recorded execution data of each executed path is applied to an arbitration module. An output result from one of the respective controllers selecting, by an arbitration module, based on the recorded execution data of each executed path. The output result of the selected controller is communicated to a next module for further processing.

Abstract: Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.