Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
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Publication number: 20140308310Abstract: The invention relates to a process for the production of 4-aminobutyric acid, also referred to as gamma-aminobutyric acid, in short GABA, to a process for the production of an extract comprising 4-aminobutyricacid, to a process for the preparation of a cosmetic formulation comprising 4-aminobutyricacid, and to the use of Cyanidium caldarium cells or cell components in cosmetic applications.Type: ApplicationFiled: July 20, 2012Publication date: October 16, 2014Applicant: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Jennifer Schild, Matthias Mentel, Peter Lersch, Mike Farwick, Christian Weitemeyer
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Publication number: 20140308717Abstract: The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.Type: ApplicationFiled: July 27, 2012Publication date: October 16, 2014Applicant: Evonik Degussa GmbHInventors: Thomas Haas, Markus Poetter, Jan Christoph Pfeffer, Wolfgang Kroutil, Arne Skerra, Alexandra Lerchner, Katharina Christin Tauber, Johann H. Sattler, Steffen Schaffer
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Patent number: 8852900Abstract: The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to synthesize (S)-3-(1-aminoethyl)-phenol and related compounds useful in the production of active pharmaceutical ingredients.Type: GrantFiled: June 16, 2011Date of Patent: October 7, 2014Assignee: Codexis, Inc.Inventors: Fabien Cabirol, Anupam Gohel, Seong Ho Oh, Derek Smith, Brian Wong, James Lalonde
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Publication number: 20140295506Abstract: A method to produce hydrazide from triacylglycerol, the method includes steps of mixing vegetable oil with an organic solvent in a reactor forming a mixture, adding hydrazinemonohydrate into the mixture, stirring the mixture, adding catalyst, stirring the mixture to form hydrazide and separating the hydrazide from the mixture.Type: ApplicationFiled: November 7, 2012Publication date: October 2, 2014Applicants: Malaysian Palm Oil Board, Universiti Putra MalaysiaInventors: Noor Maznee Tuan Ismail, Hazimah Abu Hassan, Wan Md Zin Wan Yunus
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Patent number: 8846366Abstract: The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof.Type: GrantFiled: December 11, 2007Date of Patent: September 30, 2014Assignee: DSM IP Assets BVInventors: Richard Gaisberger, Anton Glieder, Zhibin Liu, Beate Pscheidt
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Publication number: 20140287461Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.Type: ApplicationFiled: May 28, 2014Publication date: September 25, 2014Applicant: Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
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Publication number: 20140273110Abstract: Bacteria that run the beta oxidation cycle in reverse anabolic direction are provided, along with many novel primers to start the reverse cycle, pathways to make such primers, and a large variety of products produced thereby. Methods for making desired product by using such primers in the reverse pathway are also disclosed.Type: ApplicationFiled: March 6, 2014Publication date: September 18, 2014Applicant: William Marsh Rice UniversityInventors: Ramon GONZALEZ, James M. CLOMBURG
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Patent number: 8835136Abstract: Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type ?-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH.Type: GrantFiled: May 15, 2013Date of Patent: September 16, 2014Assignee: Georgia Tech Research CorporationInventors: Andreas Sebastain Bommarius, Michael Justin Abrahamson, Bettina Bommarius
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Publication number: 20140256005Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: ApplicationFiled: May 29, 2014Publication date: September 11, 2014Applicant: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20140256904Abstract: The subject of the invention is a biotechnological process for the production of ?-functionalized carboxylic acids and of ?-functionalized carboxylate esters from simple carbon sources.Type: ApplicationFiled: August 15, 2012Publication date: September 11, 2014Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Nicole Decker, Jasmin Gielen, Harald Haeger, Thomas Haas, Markus Poetter, Hans-Georg Hennemann, Mirja Wessel, Michael Volland
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Publication number: 20140248671Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.Type: ApplicationFiled: May 16, 2014Publication date: September 4, 2014Applicant: Novozymes, Inc.Inventor: Sandra Merino
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Publication number: 20140248673Abstract: This document describes biochemical pathways for producing pimelic acid, 7-hydroxyheptanoic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the CoA-dependent elongation enzymes or analog enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: September 4, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140242644Abstract: Process for the enzymatic synthesis of the compound of formula (I): Application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.Type: ApplicationFiled: February 26, 2014Publication date: August 28, 2014Applicant: LES LABORATOIRES SERVIERInventors: Sandrine Pedragosa-Moreau, François Lefoulon
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Publication number: 20140242646Abstract: The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.Type: ApplicationFiled: July 17, 2012Publication date: August 28, 2014Applicant: EVONIK DEGUSSA GmbHInventors: Markus Pötter, Thomas Haas, Jan Christoph Pfeffer, Arne Skerra, Wolfgang Kroutil, Alexandra Lerchner, Johann H. Sattler, Steffen Schaffer, Katharina Christin Tauber
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Publication number: 20140234918Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: August 21, 2014Applicant: Novozymes A/SInventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Patent number: 8809024Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure prepared by subjecting an acid of the structure to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure 2 with di-tert-butyl dicarbonate to form the BOC-protected amine.Type: GrantFiled: January 4, 2013Date of Patent: August 19, 2014Assignee: AstraZeneca ABInventors: Michael Politino, Matthew M. Cadin, Jason G. Chen
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Publication number: 20140220457Abstract: The present invention relates generally to substrates for making polymers and methods for making polymers. The present invention also relates generally to polymers and devices comprising the same.Type: ApplicationFiled: February 5, 2014Publication date: August 7, 2014Applicant: Avertica, Inc.Inventor: Nicholas Brendan Duck
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Publication number: 20140220122Abstract: The invention relates to methods, compounds, compositions and vehicles for delivering 3-amino-1-propanesulfonic acid (3APS) in a subject, preferably a human subject. The invention encompasses compound that will yield or generate 3APS, either in vitro or in vivo.Type: ApplicationFiled: April 7, 2014Publication date: August 7, 2014Applicant: BHI Limited PartnershipInventors: Xianqi KONG, Mohamed ATFANI, Benoit BACHAND, Abderrahim BOUZIDE, Stephane CIBLAT, Sophie LEVESQUE, David MIGNEAULT, Isabelle VALADE, Xinfu WU, Daniel DELORME
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Publication number: 20140212934Abstract: Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes.Type: ApplicationFiled: April 9, 2014Publication date: July 31, 2014Applicant: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia, Shaun M. Lippow
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Publication number: 20140212933Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.Type: ApplicationFiled: July 9, 2012Publication date: July 31, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140199734Abstract: The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.Type: ApplicationFiled: July 14, 2011Publication date: July 17, 2014Applicant: DSM IP ASSETS B.V.Inventors: Martin Schürmann, Wijnand Peter Helena Peeters, Natascha Hubertina Smeets, Helmut Schwab, Kerstin Steiner, Kateryna Mykoiayivna Lypetska, Gernot Strohmeier
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Publication number: 20140199736Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.Type: ApplicationFiled: July 23, 2012Publication date: July 17, 2014Applicant: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
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Publication number: 20140199737Abstract: This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.Type: ApplicationFiled: December 23, 2013Publication date: July 17, 2014Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Patent number: 8778642Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: GrantFiled: June 4, 2012Date of Patent: July 15, 2014Assignee: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Publication number: 20140193861Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á.r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193865Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193864Abstract: This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193862Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193863Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.á r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Patent number: 8771998Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: GrantFiled: February 20, 2009Date of Patent: July 8, 2014Assignee: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20140186904Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.Type: ApplicationFiled: December 23, 2013Publication date: July 3, 2014Applicant: INVISTA North America S.á r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140186884Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.Type: ApplicationFiled: July 23, 2013Publication date: July 3, 2014Applicant: BP Corporation North Americca Inc.Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
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Publication number: 20140186905Abstract: Subject matter of the invention is a biotechnological process for the production of organic compounds with the aid of at least one alkL gene product.Type: ApplicationFiled: August 15, 2012Publication date: July 3, 2014Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Jasmin Gielen, Nicole Decker, Nicole Kirchner, Thomas Haas, Markus Poetter, Harald Haeger
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Publication number: 20140186902Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: July 3, 2014Applicant: INVISTA North America S. a r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140178937Abstract: A method of producing a sugar liquid with a cellulose-containing biomass as a raw material includes (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution, and (2) filtering the aqueous sugar solution obtain in (1) through an ultrafiltration membrane having a molecular weight cutoff of 600 to 2,000 to remove a fermentation inhibitor(s) into the permeate side and collect a sugar liquid from the feed side.Type: ApplicationFiled: July 27, 2012Publication date: June 26, 2014Applicant: Toray Industries, Inc.Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
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Publication number: 20140178948Abstract: The invention provides a whole cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a ?-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.Type: ApplicationFiled: December 18, 2013Publication date: June 26, 2014Applicant: Evonik Industries AGInventors: Steffen SCHAFFER, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
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Publication number: 20140171683Abstract: The present invention relates to a novel process for converting a substrate of formula (III) and/or (IV) into a product of formula (I) or (II) comprising the following reactions: a) oxidation of at least one terminal C-atom, b) dehydratation, c) decarboxylation and d) reduction and/or amination. At least step b is enzyme-catalyzed. In a preferred embodiment, all reactions are enzymatically catalyzed. The enzymes catalyzing the reactions are selected from oxidoreductases, decarboxylases, dehydratases and/or aminotransferases. The process may be performed in a cell-free in vitro production system or in an improved fermentative production system.Type: ApplicationFiled: March 26, 2012Publication date: June 19, 2014Inventors: Volker Sieber, André Pick, Broder Rühmann
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Publication number: 20140170715Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: June 19, 2014Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Patent number: 8753850Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: July 13, 2012Date of Patent: June 17, 2014Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 8748661Abstract: The present invention relates to novel polyamino polyketide antibiotics, methods of their production as well as methods of using these antibiotics, for example, for inhibition or removal of biofilm formation or for treating bacterial infection with these antibiotics.Type: GrantFiled: November 9, 2009Date of Patent: June 10, 2014Assignee: Agency for Science, Technology and ResearchInventors: Jien Wu, Haibao Zhang, Lianhui Zhang
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Publication number: 20140154754Abstract: Described herein are methods and systems that can provide independent energy generation as well as systems for the generation of other useful products, such as chemicals. In addition, the systems and methods can provide ways of harnessing the potential of biomass feedstock to generate of multitude of products including green crude, biogas, electricity, heat, fatty acids, biodiesel, ammonia, and chemical products. In many cases, the systems and methods herein utilize anaerobic microorganisms, including aquatic and ruminant organisms, to digest material and create products.Type: ApplicationFiled: October 21, 2009Publication date: June 5, 2014Applicant: Blue Marble Energy CorporationInventor: James Stephens
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Patent number: 8741593Abstract: This invention has as its object a method for releasing a product by subjecting a compound of Formula (II?): R?7R?8(HX)C1-C2(YH)R?9R?10 to a chemical oxidation that cleaves the bond C1-C2 to obtain the product. In the compound of Formula (II?): R?7 to R?10, which are identical or different, correspond to a hydrogen atom, a substituted or unsubstituted alkyl group, or a substituted or unsubstituted functional group; X and Y, which are identical or different, are an oxygen atom, a sulfur atom, or an amine of Formula —NR11R12, wherein R11 is a hydrogen atom, an alkyl group, or a substituted or unsubstituted aryl group, and R12 is not a hydrogen atom. The invention also has as its object a method for releasing a product that comprises, before the chemical oxidation stage, a first step for preparing the compound of Formula (II?). The released product can be a volatile molecule or an active substance or else a specific product.Type: GrantFiled: November 20, 2006Date of Patent: June 3, 2014Assignee: ProteusInventors: Jean-Louis Reymond, Denis Wahler, Frabrizzio Badalassi, Hong-Khanh Nguyen
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Patent number: 8741599Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducType: GrantFiled: November 27, 2006Date of Patent: June 3, 2014Assignee: BASF SEInventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
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Patent number: 8728792Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: January 21, 2010Date of Patent: May 20, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Patent number: 8728762Abstract: The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: i) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; ii) the grinding material is suspended in an aqueous liquid in an amount such that the dry mass content in the suspension is at least 45 wt.Type: GrantFiled: November 27, 2006Date of Patent: May 20, 2014Assignee: BASF SEInventors: Matthias Boy, Stephan Freyer
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Publication number: 20140134682Abstract: Recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine or N-acetylcadaverine, as well as recombinant DNA molecules and polypeptides used to produce the microorganisms are provided. Said microorganisms comprise an intracellular lysine decarboxylase activity and a deregulated cadaverine export activity, or comprise a decreased cadaverine export activity and an enhanced N-acetylcadaverine forming activity. Processes for the production of cadaverine N-acetylcadaverine using the recombinant microorganisms are also provided.Type: ApplicationFiled: February 20, 2012Publication date: May 15, 2014Applicant: BASF SEInventors: Christoph Wittmann, Stefanie Kind
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Publication number: 20140134681Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.Type: ApplicationFiled: December 13, 2013Publication date: May 15, 2014Inventors: Petronella Catharina RAEMAKERS-FRANKEN, Martin SCHURMANN, Axel Christoph TREFZER, Stefaan Marie Andre DE WILDEMAN
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Publication number: 20140127754Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.Type: ApplicationFiled: November 5, 2012Publication date: May 8, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
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Patent number: 8715969Abstract: A method for the production of a saccharification product is provided, the method comprising: a) providing a cellulosic feedstock comprising lignin; b) contacting, in water, the feedstock of (a) with at least one metal carbonate at time 0 hour under suitable initial reaction conditions to produce a first delignification slurry; c) contacting the first delignification slurry with a nucleophilic base at time (0+n) hour, where n represents a number greater than zero, under suitable secondary reaction conditions to form a second delignification slurry comprising a lignin-containing liquid fraction and a polysaccharide-enriched solid fraction; d) separating the lignin-containing liquid fraction from the polysaccharide-enriched solid fraction; and e) contacting at least a portion of the polysaccharide-enriched solid fraction with an enzyme consortium to produce a saccharification product comprising xylose and glucose monomers wherein the overall yield of xylose and glucose monomers in the saccharification producType: GrantFiled: November 19, 2009Date of Patent: May 6, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Subramaniam Sabesan, Christina Jacy Spado
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Publication number: 20140120587Abstract: A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.Type: ApplicationFiled: January 10, 2012Publication date: May 1, 2014Applicant: Evonik Degussa GmbHInventors: Thomas Haas, Jan Christoph Pfeffer, Kurt Faber, Michael Fuchs