Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
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Publication number: 20130203126Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: April 5, 2013Publication date: August 8, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130196389Abstract: A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.Type: ApplicationFiled: September 28, 2011Publication date: August 1, 2013Applicant: KANEKA CORPORATIONInventors: Noriyuki Ito, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
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Publication number: 20130198911Abstract: The present invention relates to a process for the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.Type: ApplicationFiled: March 25, 2013Publication date: August 1, 2013Applicant: BASF Plant Science GmbHInventor: BASF Plant Science GmbH
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Patent number: 8497098Abstract: The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein in the microorganism also an increased activity of N-acetylglutamate formation is present as compared to the native level of activity of N-acetylglutamate formation in the microorganism and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. The invention also relates to vectors, plasmids and hosts carrying a corresponding increased ODC activity and an increased activity of N-acetylglutamate formation.Type: GrantFiled: July 11, 2005Date of Patent: July 30, 2013Assignee: DSM IP Assets B.V.Inventors: Katrin Eppelmann, Petrus M. M. Nossin, Leon J. R. M. Raeven, Susanne M. Kremer, Marcel G. Wubbolts
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Publication number: 20130190400Abstract: The embodiment of invention provides a method for producing ?-aminobutyric acid, including the steps of providing a medium including Saccharomyces cerevisiae, adding an extract of Rhinacanthus nasutus into the medium and fermentating Saccharomyces cerevisiae in the medium added with the extract of Rhinacanthus nasutus to produce ?-aminobutyric acid.Type: ApplicationFiled: January 19, 2012Publication date: July 25, 2013Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Ching-Hui CHANG, Tsai-Mei WU
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Publication number: 20130183725Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.Type: ApplicationFiled: February 12, 2013Publication date: July 18, 2013Inventors: MARKUS POETTER, HANS-GEORG HENNEMANN, STEFFEN SCHAFFER, THOMAS HAAS
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Publication number: 20130183728Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 14, 2012Publication date: July 18, 2013Applicant: INVISTA NORTH AMERICA S.A R.L.Inventor: INVISTA North America S.a.r.l.
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Publication number: 20130177951Abstract: A process for producing surface-active quaternary ammonium esters is provided. These esters are advantageously prepared in high yield and purity by a chemo-enzymatic process. These compounds have excellent surfactant properties.Type: ApplicationFiled: January 6, 2012Publication date: July 11, 2013Applicant: EASTMAN CHEMICAL COMPANYInventors: Christopher Harlan Burk, Neil Warren Boaz, Stephanie Kay Clendennen
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Patent number: 8481293Abstract: Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.Type: GrantFiled: October 14, 2009Date of Patent: July 9, 2013Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Yong Jae Jeon
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Patent number: 8481294Abstract: Durability of formate dehydrogenase is improved with the use of formate dehydrogenase exhibiting high specific activity that is unpredictable from conventional findings. A specific amino acid substitution is introduced into Gibberella zeae-derived formate dehydrogenase. Mutant formate dehydrogenase exhibits durability that is extremely superior to that of wild-type formate dehydrogenase. Thus, the productivity of NADH that is produced using the mutant formate dehydrogenase can be improved.Type: GrantFiled: August 3, 2009Date of Patent: July 9, 2013Assignee: Toyota Jidosha Kabushiki KaishaInventor: Takahiro Kusumegi
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Publication number: 20130164794Abstract: The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.Type: ApplicationFiled: August 8, 2011Publication date: June 27, 2013Applicant: CODEXIS, INC.Inventors: John Limanto, Gregory Beutner, Brendan Grau, Jacob Janey, Artis Klapars, Eric Ashley, Hallena R. Strotman, Matthew D. Truppo, Gregory Hughes, Fabien L. Cabirol, Anupam Gohel, Steven J. Collier, Jack Liang, Marissa Mock, Emily Mundorff, Scott Novick, Derek Smith
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Patent number: 8470564Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: January 8, 2010Date of Patent: June 25, 2013Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Gregory Miller, Xiyun Zhang
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Publication number: 20130157323Abstract: The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.Type: ApplicationFiled: August 3, 2011Publication date: June 20, 2013Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Min Young Kim
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Publication number: 20130149752Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.Type: ApplicationFiled: November 19, 2012Publication date: June 13, 2013Applicant: EVOCATAL GMBHInventor: EVOCATAL GMBH
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Publication number: 20130130333Abstract: The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters.Type: ApplicationFiled: January 25, 2013Publication date: May 23, 2013Applicant: VERENIUM CORPORATIONInventor: Verenium Corporation
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Publication number: 20130115663Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: ApplicationFiled: October 23, 2012Publication date: May 9, 2013Applicant: CODEXIS, INC.Inventor: Codexis, Inc.
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Patent number: 8431378Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.Type: GrantFiled: January 27, 2012Date of Patent: April 30, 2013Assignee: Kaneka CorporationInventors: Noriyuki Ito, Yoshihiko Yasohara
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Patent number: 8426178Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2- thienyl)-1-propanamine.Type: GrantFiled: August 27, 2009Date of Patent: April 23, 2013Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20130095534Abstract: By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.Type: ApplicationFiled: February 22, 2011Publication date: April 18, 2013Applicant: TORAY INDUSTRIES INCInventors: Takashi Mimitsuka, Kazumi Suda, Hideki Sawai
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Publication number: 20130089898Abstract: The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to synthesize (S)-3-(1-aminoethyl)-phenol and related compounds useful in the production of active pharmaceutical ingredients.Type: ApplicationFiled: June 16, 2011Publication date: April 11, 2013Applicant: CODEXIS, INC.Inventors: Fabien Cabirol, Anupam Gohel, Seong Ho Oh, Derek Smith, Brian Wong, James Lalonde
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Publication number: 20130090445Abstract: The present invention provides a synthesis system that can synthesize aniline and/or styrene efficiently, a synthesis system that can synthesize butadiene (1,3-butadiene) efficiently, a rubber chemical for a tire which is synthesized from the aniline obtained by the synthesis system, a synthetic rubber for a tire which is synthesized from the styrene and/or butadiene obtained by the synthesis systems, and a pneumatic tire produced using the rubber chemical for a tire and/or the synthetic rubber for a tire. The present invention relates to a synthesis system for synthesizing aniline and/or styrene from an alcohol having two or more carbon atoms via an aromatic compound.Type: ApplicationFiled: January 25, 2012Publication date: April 11, 2013Applicant: SUMITOMO RUBBER INDUSTRIES, LTD.Inventors: Takayuki Hattori, Takao Wada, Keitaro Fujikura, Yuka Yokoyama, Toshiro Matsuo
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Publication number: 20130084608Abstract: The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.Type: ApplicationFiled: August 29, 2012Publication date: April 4, 2013Applicant: Codexis, Inc.Inventors: Lorand Szabo, Zsolt Molnar, Attila Laszlo Nemeth
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Publication number: 20130079486Abstract: In a method for producing 1,5-pentamethylenediamine, a lysine decarboxylase-expressing microorganism that is subjected to a treatment is used.Type: ApplicationFiled: February 25, 2011Publication date: March 28, 2013Inventors: Tomonori Hidesaki, Akiko Natsuji, Toshihiko Nakagawa, Goro Kuwamura, Daisuke Hasegawa, Satoshi Yamasaki, Kuniaki Sato, Hiroshi Takeuchi
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Publication number: 20130071888Abstract: A method of producing 1,5-pentanediamine includes culturing coryneform bacterium having a gene encoding lysine decarboxylase in its chromosome, which coryneform bacterium maintains lysine decarboxylase activity of not less than 50 mU/mg protein during culturing and the gene encoding lysine decarboxylase is linked downstream of a promoter that functions during the logarithmic growth phase.Type: ApplicationFiled: April 11, 2011Publication date: March 21, 2013Applicant: TORAY INDUSTRIES, INC.Inventors: Kenji Sawai, Shiomi Watanabe, Takashi Mimitsuka, Hideki Sawai
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Patent number: 8399225Abstract: The invention relates to a process for preparing urethane-containing acrylic- and (meth)acrylic esters by reacting a urethane-containing alcohol with a reactant acrylic- or (meth)acrylic ester of a saturated alcohol in the presence of at least one polymerization inhibitor and an enzyme as a catalyst within a reactor, wherein the saturated alcohol released in the process and optionally an entraining agent form an azeotrope with an excess of the reactant (meth)acrylic ester, such that the azeotrope is removed by distillation under reduced pressure and at least one substream from the bottom of the reactor is circulated through the top of the distillation column. Using this process, urethane-containing acrylic- and (meth)acrylic esters are prepared in high yields and high purities under mild conditions from economically obtainable reactants with no significant polymer formation.Type: GrantFiled: May 10, 2010Date of Patent: March 19, 2013Assignee: BASF SEInventors: Rainer Stürmer, Dejana Drew, Bernhard Hauer, Jürgen Däuwel, Uwe Meisenburg
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Publication number: 20130059331Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.Type: ApplicationFiled: April 19, 2011Publication date: March 7, 2013Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
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Publication number: 20130059332Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.Type: ApplicationFiled: September 14, 2012Publication date: March 7, 2013Applicant: BASF SEInventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jae Yeol Seo, Jörg Braun, Mo Se Kim, Sung Hyun Kim, Arno Kochner
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Publication number: 20130052698Abstract: The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.Type: ApplicationFiled: August 22, 2012Publication date: February 28, 2013Applicant: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Jungjoo Yoon, Kripa Rao, John H. Grate, David Elgart, Dipnath Baidyaroy
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Publication number: 20130052700Abstract: The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.Type: ApplicationFiled: March 15, 2011Publication date: February 28, 2013Inventors: Markus Poetter, Andreas Schmid, Bruno Buehler, Hans-Georg Hennemann, Mattijs Kamiel Julsing, Steffen Schaffer, Thomas Haas, Manfred Schrewe, Sjef Cornelissen, Martin Roos, Harald Haeger
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Patent number: 8377662Abstract: The document describes a process for the preparation of N-Acyl-Phosphatidyl-Ethanolamine of formula (I) on an industrial scale, In which R1, R2 and R3 are, independently from each other, saturated, monounsaturated or polyunsaturated acyls C10-C30, pure or mixed together, and X?OH or OM, where M=alkaline metal or alkaline earth, ammonium or alkylammonium. The process in question allows the conversion of lecithin of synthetic or natural origin into N-Acyl-Phosphatidyl-Ethanolamine of formula (I) of high purity, using a limited molar excess of the reagent N-acyl-ethanolamine, where the acyl is as defined above for the formula (I) through reaction of transphosphatidylation in the presence of the enzyme phospholipase D and in conditions suitable for production on an industrial scale.Type: GrantFiled: July 8, 2008Date of Patent: February 19, 2013Assignee: Chemi S.p.A.Inventors: Lorenzo De Ferra, Mauro Anibaldi, Ettore Ammirati
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Patent number: 8377680Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: May 7, 2010Date of Patent: February 19, 2013Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 8377661Abstract: The invention relates to a method for synthesizing ?-amino-alkanoic acids or the esters thereof from mono-unsaturated natural fatty acids comprising at least one step of forming the unsaturated diacid corresponding to the original fatty acid.Type: GrantFiled: July 9, 2009Date of Patent: February 19, 2013Assignee: Arkema FranceInventor: Jean-Luc Dubois
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Patent number: 8372601Abstract: The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.Type: GrantFiled: January 21, 2011Date of Patent: February 12, 2013Assignee: University of Illinois at Urbana-ChampaignInventors: William W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benjamin T. Circello, Svetlana A. Borisova
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Patent number: 8372608Abstract: The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters.Type: GrantFiled: March 22, 2011Date of Patent: February 12, 2013Assignee: Verenium CorporationInventors: Mark J. Burk, Grace DeSantis, Brian Morgan, Zuolin Zhu
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Publication number: 20130034885Abstract: Subject of the invention is a process for the production of L-carnitine, wherein a ?-lactone, which is a 4-(halomethyl)oxetane-2-one, is converted into L-carnitine, wherein the process comprises an enzymatic conversion of the ?-lactone into (R)-4-halo-3-hydroxybutyric acid or (R)-4-halo-3-hydroxybutyric acid ester.Type: ApplicationFiled: July 25, 2012Publication date: February 7, 2013Inventors: Ellen Klegraf, Manuela Avi
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Publication number: 20130031676Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 11, 2011Publication date: January 31, 2013Applicant: Novozymes A/SInventors: Ming Li, Junxin Duan, Zheng Liu, Shiro Fukuyama, Keiichi Ayabe, Guillermo Coward-Kelly, Randall Deinhammer
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Publication number: 20130029381Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.Type: ApplicationFiled: April 13, 2012Publication date: January 31, 2013Applicant: GENOMATICA, INC.Inventors: Robert Haselbeck, John D. Trawick, Wei Niu, Anthony P. Burgard
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Patent number: 8361761Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure prepared by subjecting an acid of the structure to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure 2 with di-tert-butyl dicarbonate to form the BOC-protected amine.Type: GrantFiled: June 14, 2012Date of Patent: January 29, 2013Assignee: Bristol Myers-Squibb CompanyInventors: Michael Politino, Matthew M. Cadin, Paul M. Skonezny, Jason G. Chen
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Publication number: 20130018124Abstract: A process for making biodegradable a synthetic polymeric material thanks to the addition of one or more yeasts to the synthetic material is disclosed.Type: ApplicationFiled: January 25, 2011Publication date: January 17, 2013Applicant: QUEENSBROOK LIMITEDInventor: Madrisano Catinari
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Publication number: 20130018051Abstract: Bridged bicyclic heteroaryl substituted triazoles and pharmaceutical compositions containing the compounds are disclosed as being useful in inhibiting the activity of the receptor protein tyrosine kinase Axl. Methods of using the compounds in treating diseases or conditions associated with Axl activity are also disclosed.Type: ApplicationFiled: September 14, 2012Publication date: January 17, 2013Applicant: Rigel Pharmaceuticals, Inc.Inventors: Rajinder Singh, Sacha Holland, Joane Litvak, Dane Goff, Jing Zhang, Thilo J. Heckrodt
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Publication number: 20130005002Abstract: Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which R1 stands for alkyl, alkoxyalkyl, alkenyl, cycloalkyl, heterocycloalkyl, aryl, or hetaryl, and R2 stands for alkyl, cycloalkyl or aryl, in which an enantiomeric mixture of a simply N-acylated 3-aminocarboxylic acid ester of general formula (I.b), in which R1 and R2 have the meanings given above and R3 stands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim 1.Type: ApplicationFiled: September 24, 2010Publication date: January 3, 2013Applicant: BASF SEInventors: Bernhard Hauer, Thomas Friedrich, Rainer Stürmer, Nina Schneider, Susanne Krauser, Wolf-Rüdiger Krahnert
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Publication number: 20130004998Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.Type: ApplicationFiled: June 22, 2012Publication date: January 3, 2013Applicant: Codexis, Inc.Inventors: Ezhilkani Subbian, John H. Grate, Catherine M. Cho, Benjamin Mijts, Jeanne Bonomo Benoit
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Publication number: 20120329095Abstract: Provided is a method of producing succinic acid in which a microorganism having a succinic acid-producing ability is allowed to react with a sugar, the method being characterized in that the ratio of the oxygen transfer rate to the succinic acid production rate (mmol-O2/mol-SA) is 0.1 to 240 and that the doubling time of the microorganism during the reaction is not shorter than 40 hours.Type: ApplicationFiled: September 7, 2012Publication date: December 27, 2012Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Katsunori YOSHIKAWA, Daisuke KIDO, Makoto MURASE
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Publication number: 20120329108Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: September 5, 2012Publication date: December 27, 2012Applicant: CODEXIS, INC.Inventors: Christopher Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Publication number: 20120309989Abstract: The invention concerns A continuous process for the production of compounds having the general formula (6): wherein R is a straight or branched chain alkyl group, R1 is a straight or branched chain alkyl group substituted with a nitrile group, a hydroxy group or a halogen atom, R2 is a hydroxy group or a keto group, and each R3 is, independently, hydrogen or a straight or branched chain alkyl group, comprising providing to a reaction zone a continuous stream of an alkyl acetate and a continuous stream of an alkali metal or alkaline earth metal amide base and contacting the continuous streams together in the reaction zone to yield an enolate compound, providing to the or a separate reaction zone a continuous stream of a compound of formula (5): wherein R1 is as previously defined, and contacting the continuous stream of compound (5) with a continuous stream of the enolate (4) in the or the separate reaction zone at a temperature above 20° C.Type: ApplicationFiled: October 22, 2010Publication date: December 6, 2012Applicant: PHOENIX CHEMICALS LIMITEDInventors: Peter McCormack, Antony John Warr, Elliot Latham
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Publication number: 20120309062Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: ApplicationFiled: June 15, 2012Publication date: December 6, 2012Applicant: Genomatica, Inc.Inventors: Anthony P. BURGARD, Priti PHARKYA, Robin E. OSTERHOUT
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Patent number: 8323942Abstract: The present invention is directed to methods for inducing desired activity in enzymes or microorganisms capable of producing the enzymes. The invention is further directed to methods of stabilizing activity in microorganisms. In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase I activity. The invention further provides compositions comprising enzymes or microorganisms having induced and/or stabilized activity.Type: GrantFiled: April 3, 2009Date of Patent: December 4, 2012Assignee: Georgia State University Research FoundationInventors: George E. Pierce, Gene K. Drago, Sangeeta Ganguly
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Patent number: 8318914Abstract: The present invention relates to polynucleotides from Pythium irregulare, Rhizopus oryzae and Euglena gracilis which code for elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: GrantFiled: July 30, 2008Date of Patent: November 27, 2012Assignees: Bioriginal Food & Science Corp., BASF Plant Science GmbHInventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20120295317Abstract: The present invention relates to the use of recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine, as well as to recombinant DNA molecules and polypeptides used to produce the microorganism, said microorganism comprising an intracellular lysine decarboxylase activity and an enhanced lysine import activity or comprising an intracellular and an extracellular lysine decarboxylase activity or comprising an intracellular and extracellular lysine decarboxylase activity and an enhanced lysine import activity. The present invention also relates to a processes for the production of cadaverine using recombinant microorganisms.Type: ApplicationFiled: December 15, 2010Publication date: November 22, 2012Applicant: BASF SEInventors: Hartwig Schröder, Weol Kyu Jeong, Annegret Serwe, Oskar Zelder
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Publication number: 20120297507Abstract: A method for producing leafy biomass from undifferentiated plant cells, the method comprising providing undifferentiated plant cells, contacting them with an agent that promotes differentiation of the cells into leafy tissue and growing the cells in a temporary liquid immersion culture system. This method of the invention may be used to produce polypeptides, and natural medicinal products, and can be used to capture carbon dioxide. A method of producing a polypeptide in plant cells in vitro comprising: providing undifferentiated plant cells containing chloroplasts that carry a transgenic nucleic acid molecule encoding the polypeptide, wherein the plant cells display homoplastomy; and propagating the cells according to the above method to produce leafy biomass containing the polypeptide.Type: ApplicationFiled: August 12, 2010Publication date: November 22, 2012Applicant: Imperial Innovations LimitedInventors: Franck Michoux, Peter Nixon, James Gerard McCarthy