Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
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Publication number: 20100319091Abstract: A method of enhancing production of a phenylpropanoid compound in a plant or plant cell is disclosed. The method comprising: (a) expressing in the plant or plant cell a heterologous polynucleotide which produces a myb gene of the phenylpropanoid pathway; and (b) contacting the plant or plant cell with at least one substrate of the phenylpropanoid pathway, the substrate being upstream to the production of the phenylpropanoid compound, thereby enhancing the production of a phenylpropanoid compound in the plant or plant cell.Type: ApplicationFiled: November 13, 2008Publication date: December 16, 2010Applicant: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREWInventors: Alexander Vainstein, Michal Moyal-Ben-Zvi, Ben Rimon-Spitzer
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Publication number: 20100317069Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: ApplicationFiled: May 7, 2010Publication date: December 16, 2010Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Publication number: 20100312003Abstract: The present invention relates to a process for the preparation of organic nitrates having at least one nitryloxy and at least one hydroxy group, wherein the at least one hydroxy group may be present in form of an esterified hydroxy residue, the latter being esterified with an acid other than nitric acid.Type: ApplicationFiled: October 10, 2008Publication date: December 9, 2010Inventors: Walter Brieden, Dominique Michel, Wilhelm Quittmann, Fabio Rainone, Jie-Hui Pan, Hang-Bing Fang, Ying-Xia Song
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Patent number: 7838267Abstract: A method of increasing the rate of conversion of reactants to reaction product of enzyme catalyzed, reversible, i.e., equilibrium, reactions having water or methanol as byproduct includes removing water and/or methanol from the reaction mass during reaction by permeation of the reaction mass through a selectively permeable perfluorinated polymer or copolymer membrane.Type: GrantFiled: October 26, 2007Date of Patent: November 23, 2010Assignees: CMS Technologies Holdings, Inc., Kansas State University Research FoundationInventors: Stuart Nemser, Mary E. Rezac
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Publication number: 20100292429Abstract: The invention relates to a method for isolating 1,5-diaminopentane (DAP) from DAP-containing fermentation broths, to a method for the fermentative production of DAP using said isolating method and to a method for producing DAP-comprising polymers by using the DAP isolated or fermatively produced DAPs according to said methods.Type: ApplicationFiled: January 23, 2009Publication date: November 18, 2010Applicant: BASF SEInventors: Martin Völkert, Oskar Zelder, Burkhard Ernst, Weol Kyu Jeong
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Publication number: 20100291640Abstract: A process for preparing urethane-containing (meth)acrylic esters (U) by reacting a urethane-containing alcohol (A) with a (meth)acrylic ester of a saturated alcohol (G) in the presence of at least one polymerization inhibitor (P) with an enzyme (E) as a catalyst in a reactor, wherein a) the saturated alcohol released and any entraining agent used form an azeotrope with the excess corresponding (meth)acrylic ester (G), the azeotrope is removed by distillation under reduced pressure and b) at least a substream from the bottom of the reactor is circulated via the top of the distillation column.Type: ApplicationFiled: May 10, 2010Publication date: November 18, 2010Applicant: BASF SEInventors: Rainer Stuermer, Dejana Drew, Bernhard Hauer, Juergen Daeuwel, Uwe Meisenburg
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Publication number: 20100291642Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure prepared by subjecting an acid of the structure to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure 2 with di-tert-butyl dicarbonate to form the BOC-protected amine.Type: ApplicationFiled: June 17, 2010Publication date: November 18, 2010Applicant: BRISTOL-MYERS SQUIBB COMPANYInventors: Michael Politino, Matthew M. Cadin, Paul M. Skonezny, Jason G. Chen
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Publication number: 20100291641Abstract: A process for preparing urethane-containing (meth)acrylic esters (U) by reacting a urethane-containing alcohol (A) with a (meth)acrylic ester of a saturated alcohol (G) in the presence of at least one polymerization inhibitor (P) with an enzyme (E) as a catalyst in a reactor, wherein the (meth)acrylic ester of a saturated alcohol (G) and the urethane-containing alcohol (A) are passed continuously through at least one fixed bed reactor filled with an immobilized enzyme (E) as a catalyst.Type: ApplicationFiled: May 12, 2010Publication date: November 18, 2010Applicant: BASF SEInventors: Jürgen Däuwel, Steffen Maurer, Rainer Stürmer
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Publication number: 20100285544Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.Type: ApplicationFiled: May 22, 2006Publication date: November 11, 2010Inventors: Noriyuki Ito, Yoshihiko Yasohara
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Publication number: 20100285541Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: February 26, 2010Publication date: November 11, 2010Applicant: CODEXIS, INC.Inventors: Christopher Saville, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Publication number: 20100273659Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: February 29, 2008Publication date: October 28, 2010Applicant: VERENIUM CORPORATIONInventors: Grace DeSantis, Jennifer Chaplin, Ellen Chi, Aileen Milan, Jay Short, David Weiner, Mark Madden, Darcy Madden, Dan Robertson
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Patent number: 7820416Abstract: In a method for producing an amide compound of the present invention including obtaining an amide compound from a nitrile compound using a microbial catalyst and transferring a solution containing the microbial catalyst and the amide compound, a positive-displacement pump is used for transferring the solution containing a microbial catalyst and an amide compound to obtain an amide compound having few impurities. A monomer including (meth)acrylamide obtained by the method for producing an amide compound is polymerized to prepare an acrylamide polymer having a high-molecular mass and high solubility and being colorless.Type: GrantFiled: December 28, 2005Date of Patent: October 26, 2010Assignee: Dia-Nitrix Co., Ltd.Inventors: Katsuo Ishii, Kozo Murao
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Publication number: 20100254948Abstract: This invention relates to a process for the production of gamma-aminobutyric acid (GABA) from Lactococcus lactis ssp. DSM 19464 and from Lactobacillus plantarum DSM 19463 or from their associations on must. In particular, this invention contemplates the selection and use of Lactococcus lactis ssp. DSM 19464 and of Lactobacillus plantarum DSM 19463 or their associations on must whose composition has been suitably optimized as to its composition, for the production of a preparation based on GABA, containing also vitamins, minerals, polyphenols and alive vital lactic bacteria for its potential use in the dermatological field.Type: ApplicationFiled: July 17, 2008Publication date: October 7, 2010Applicant: Giuliani S.p.AInventors: Giammaria Giuliani, Anna Benedusi, Raffaella Di Cagno, Carlo Giuseppe Rizzello, Maria De Angelis, Francesco Mazzacane, Marco Gobbetti
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Patent number: 7807423Abstract: The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives The present invention also provides methods and compositions for preparing vicinal cyano, hydroxyl substituted carboxylic acid esters.Type: GrantFiled: August 10, 2006Date of Patent: October 5, 2010Assignee: Codexis, Inc.Inventors: S. Christopher Davis, John H. Grate, David R. Gray, John M. Gruber, Gjalt W. Huisman, Steven K. Ma, Lisa M. Newman, Roger Sheldon, Li A Wang
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Publication number: 20100248317Abstract: A process for the enantioselective enzymatic reduction of a keto compound of general formula I wherein R may represent any protective group for amino functions (tert. butyloxycarbonyl group (BOC), benzyloxycarbonyl group, 9-fluorenylmethoxycarbonyl group) and X?—Cl, —CN, —OH, Br, F.Type: ApplicationFiled: September 22, 2008Publication date: September 30, 2010Applicant: IEP GMBHInventors: Antje Gupta, Maria Bobkova, Anke Tschentscher
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Publication number: 20100240105Abstract: A method for producing glucosamine with microorganism comprises of fermenting with a microorganism selected from the group consisting of Monascus pilosus and Aspergillus sp. in a novel low-cost medium, thereby enable it to produce glucosamine; wherein said medium is consisted of commercial Taiwan sugar, soy beam, rice bran and the like; wherein suitable condition for the fermentation is: 150˜300 rpm, pH 4˜pH 8, and 24° C.˜37° C.; wherein, after fermentation culturing, the fermentation liquor is filtered with suction to recover said microorganism biomass, said microorganism biomass is then subjected to steps of cell disruption, hydrochloric acid reaction, neutralization reaction and filtration, to obtain glucosamine produced by the microorganism.Type: ApplicationFiled: March 19, 2009Publication date: September 23, 2010Applicant: Yuan Ze UniversityInventors: Ho-Shing Wu, Yu-Fen Chang, Yu-Chiao Wei
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Publication number: 20100227935Abstract: The present invention relates to a new class of terpene-derived compounds having an antibiotic activity and of the formulae (I) or (II) in which A is selected from NR1, O, S, CR2R3 or R—(CH2)n—R?, wherein R and R? represent independently NH, O, S or CH2, and in which R1, R2 et R3 are substituents and n is higher than or equal to 1, in particular equal to 2. The invention relates to the compound according to both the formula (I) or the formula (II) taken individually, to mixtures of the two compounds, and to compositions, mainly therapeutic ones, comprising at least one of the compounds or said mixture. According to a particular embodiment, the compound ((I) or (II) taken individually, the mixtures of both compounds and the compositions of the invention are used in the treatment of difficult C-related digestive infections. In one particular embodiment, the compound of the formula (I) is margaucine, a compound produced by a bacterial stem.Type: ApplicationFiled: September 21, 2007Publication date: September 9, 2010Applicants: Centre National De La Recherche Scientifique, Universite De Provence-Aix-Marseille 1, Universite Paul Cezanne-Aix-Marseille 3Inventors: Jean-Paul Leonetti, Maxime Gualtieri, Laurence Coulibeuf, Gaëtan Herbette
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Publication number: 20100215620Abstract: The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.Type: ApplicationFiled: October 23, 2008Publication date: August 26, 2010Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Hyun Ok Yang, Hak Cheol Kwon, Hyun-Bong Park, Ji-Hye Yoo
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Publication number: 20100209981Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: ApplicationFiled: January 8, 2010Publication date: August 19, 2010Applicant: CODEXIS, INC.Inventors: ISH KUMAR DHAWAN, GREGORY MILLER, XIYUN ZHANG
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Publication number: 20100209980Abstract: The invention relates to a method for the enantioselective N-acylation of aminoalkylphenols, and to a method for producing enantiomer-pure compounds of formulae (I-S) and/or (I-R).Type: ApplicationFiled: June 19, 2006Publication date: August 19, 2010Applicant: BASF AktiengesellschaftInventor: Klaus Ditrich
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Publication number: 20100203599Abstract: Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications.Type: ApplicationFiled: October 14, 2009Publication date: August 12, 2010Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Yong Jae Jeon
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Publication number: 20100203600Abstract: The invention relates to a method for converting ricinoleic acid that comprises the following successive stages: a) fermentation of ricinoleic acid by a bacterium, a yeast or a fungus, in order to obtain 7-hydroxy-9-octadecenedioic diacid, b) the esterification of the 7-hydroxy-9-octadecenedioic diacid obtained in-stage during step a), in order to form the corresponding methyl diester, c) high-temperature cracking of the product obtained during step b), in order to form a mixture of methyl undecylenate and methyl 7-oxoheptanoate, d) separating the methyl undecylenate and methyl 7-oxoheptanoate obtained in step c), e) hydrolysing the methyl undecylenate obtained in step d), for forming the undecylenic acid.Type: ApplicationFiled: September 17, 2008Publication date: August 12, 2010Applicant: Arkema FranceInventor: Jean-Luc Dubois
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Publication number: 20100196964Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.Type: ApplicationFiled: July 4, 2008Publication date: August 5, 2010Applicant: BASF SEInventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jai Yeol Seo, Jörg Braun, Mo Se Kim, Sung-Hyun Kim, Arno Kochner
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Publication number: 20100192238Abstract: The present invention relates to polynucleotides from Pythium irregulare, Rhizopus oryzae and Euglena gracilis which code for elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: July 30, 2008Publication date: July 29, 2010Applicants: BIORIGINAL FOOD & SCIENCE CORP., BASF PLANT SCIENCE GMBHInventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20100190218Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: ApplicationFiled: December 23, 2009Publication date: July 29, 2010Applicant: CODEXIS, INC.Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
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Patent number: 7759095Abstract: Process for the preparation of phosphatidylserine of formula wherein R1 and R2 independently represent a saturated, mono-unsaturated or polyunsaturated acyl C10-C30, X=OH or OM where M=alkaline or alkaline earth metal, ammonium, alkylammonium (including the inner salt) including the transphosphatidylation reaction between phosphatidylcholine of the general formula wherein R1 and R2 and X have the above specified meanings, R3=CH2—CH2—NH2 o CH2—CH2—N+(CH3)3 and Serine in D, L or racemic form catalized by the phospholipase D enzyme (PLD), characterized in that said reaction is carried out in a hydroalcoholic medium containing an aliphatic alcohol and in the presence of bivalent metal oxide.Type: GrantFiled: May 26, 2006Date of Patent: July 20, 2010Assignee: Fidia Farmaceutici S.p.A.Inventors: Anna Maria Zanellato, Mara Pittarello, Antonio Gambillara, Susanna Vaccaro
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Publication number: 20100173369Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: ApplicationFiled: August 27, 2009Publication date: July 8, 2010Applicant: CODEXIS, INC.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20100170010Abstract: The invention relates to the field of secondary metabolite production in plants and plant cell cultures. More specifically, the invention relates to the use of transporters and more particularly ABC-transporters to enhance the production and/or secretion of secondary metabolites in plants and plant cell cultures.Type: ApplicationFiled: November 24, 2009Publication date: July 1, 2010Inventors: Alain Goossens, Dirk G Inzé
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Publication number: 20100168453Abstract: The invention relates to a method for the synthesis of amino acids/esters of general formula NH2—(CH2)n—COOR in which n is an integer between 5 and 14, and R is either H or an alkyl radical including from 1 to 4 carbon atoms, from natural long-chain mono-unsaturated fatty acids or esters including at least 10 adjacent carbon atoms per molecule, said method comprising: first converting, if necessary, said natural long-chain fatty acid or ester into a monounsaturated fatty acid/ester of general formula R1—(CH2)m—CH?CH—(CH2)p—COOR in which R1 is H, CH3 or a COOR radical, m is an integer between 0 and 14 and p is an integer between 2 and 11, then submitting the latter to a crossed catalytic metathesis reaction with a compound of formula R2—CH?CH—R3 in which R2 is either H or CN and R3 is CN or CH2NH2, provided that if R2 is CN, R3 can be only CN, and finally converting the resulting product of the general formula R3—CH?CH—(CH2)p—COOR into an amino-acid, either by hydrogenation, or by hydrogenation of the triple tType: ApplicationFiled: February 15, 2008Publication date: July 1, 2010Applicant: Arkema FranceInventor: Jean-Luc Dubois
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Patent number: 7741082Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure prepared by subjecting an acid of the structure to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure 2 with di-tert-butyl dicarbonate to form the BOC-protected amine.Type: GrantFiled: April 12, 2005Date of Patent: June 22, 2010Assignee: Bristol-Myers Squibb CompanyInventors: Michael Politino, Matthew M. Cadin, Paul M. Skonezny, Jason G. Chen
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Publication number: 20100151534Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: ApplicationFiled: August 27, 2009Publication date: June 17, 2010Applicant: CODEXIS, INC.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20100151537Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: ApplicationFiled: January 21, 2010Publication date: June 17, 2010Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20100151528Abstract: This disclosure relates to validamycin A biosynthesis and in particular, to methods of producing validamycin A analogs and uses thereof. In a particular example, a method for making a validamycin A analog includes transforming a host cell with one or more recombinant DNA vectors to produce a valN-inactivated mutant; and culturing the valN-inactivated mutant in a culture medium to produce a validamycin A analog, such as 1,1?-bis-valienamine and validienamycin, and their conversion to valienamine. The present disclosure further relates to compositions including such compounds as well as methods of using the compositions, such as for antifungal agents.Type: ApplicationFiled: September 15, 2009Publication date: June 17, 2010Inventors: Taifo Mahmud, Zixin Deng, Linquan Bai, Hui Xu, Jongtae Yang
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Publication number: 20100151536Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: ApplicationFiled: December 14, 2009Publication date: June 17, 2010Inventors: Brian M. Baynes, John Michael Geremia
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Publication number: 20100151535Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: ApplicationFiled: November 30, 2009Publication date: June 17, 2010Applicant: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Publication number: 20100143986Abstract: The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof.Type: ApplicationFiled: December 11, 2007Publication date: June 10, 2010Inventors: Richard Gaisberger, Anton Glieder, Zhibin Liu, Beate Pscheidt
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Publication number: 20100137636Abstract: The present disclosure relates to methods of rational genome mining. A method may include narrowing the number of clones that would otherwise need to be screened and/or identifying a gene with a desired catalytic activity. The disclosure also relates to a nitrile hydrolase from Bradyrhizobium japonicum USDA110 first identified by rational genome mining. In addition, the disclosure relates to nitrilase bll6402 and catalytically active variants capable of converting an ?-hydroxy nitriles, a ?-hydroxy nitrile and/or an ?,?-dinitrile to a carboxylic acid.Type: ApplicationFiled: June 9, 2009Publication date: June 3, 2010Inventors: Dunming Zhu, Ling Hua, Edward R. Biehl, Chandrani Mukherjee
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Publication number: 20100136617Abstract: The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.Type: ApplicationFiled: March 7, 2008Publication date: June 3, 2010Applicant: MITSUBISHI RAYON CO., LTD.Inventors: Takanori Akiyama, Fujio Yu, Fumiaki Watanabe, Yuya Takikawa, Eiji Sato
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Patent number: 7727749Abstract: The present invention is directed to a regio- and stereoselective bioconversion of selected aliphatic dinitriles into corresponding cyanocarboxylic acids. More particularly, the present invention provides methods for the conversion of 2-isobutyl-succinonitrile into (S)-3 cyano-5-methylhexanoic acid, which is a useful intermediate in the synthesis of (S)-3(aminomethyl)-5-methylhexanoic acid (pregabalin). Pregabalin can be used for treating certain cerebral diseases, for example, in the treatment and prevention of seizure disorders, pain, and psychotic disorders.Type: GrantFiled: April 1, 2005Date of Patent: June 1, 2010Assignee: Pfizer, Inc.Inventors: Michael P. Burns, Justin K. Weaver, John Wing Wong
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Patent number: 7723065Abstract: Organic compounds, such as precursors for aryl ethylamines such as ephedrine, aryl propylamines such as fluoxetine and propionic acid derivatives such as ibuprofen, naproxen and fenoprofen, are subjected to a yeast mediated reduction conducted in the absence of a solvent. The yeast is moistened with water and contacted with the organic compound. The yeast may then be contacted with an organic solvent to dissolve the product of the reaction into the solvent, and a solid/liquid separation used to separate the product from the yeast.Type: GrantFiled: March 31, 2004Date of Patent: May 25, 2010Assignee: Victoria UniversityInventors: Maurice Arthur Trewhella, Nick Athanasiou, Andrew John Smallridge
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Patent number: 7718414Abstract: Provided is a microorganism that belongs to Enterobacteriacae and a method of producing L-carnitine using the same. The microorganism includes polynucleotide encoding activity of S-adenosylmethionine-6-N-iysine methyltransferase from Neurospora crassa, polynucleotide encoding activity of 6-N-trimethyllysine hydroxylase, polynucleotide encoding activity of 3-hydroxy-6-N-trimethyllysine aldolase, and polynucleotide encoding activity of ?-trimethylaminoaldehyde dehydrogenase and y-butyrobetaine hydroxylase.Type: GrantFiled: July 7, 2006Date of Patent: May 18, 2010Assignee: CJ Cheiljedang Corp.Inventors: Whan-Koo Kang, Bheong-Uk Lee, Young-Hoon Park, Eun-Sung Koh, Jae-Yeong Ju, Jin-Ho Lee, Hye-Won Kim, Hye-Jin Choi
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Publication number: 20100112648Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.Type: ApplicationFiled: December 10, 2007Publication date: May 6, 2010Applicant: EVOCATAL GMBHInventors: Thorsten Eggert, Jennifer Andexer
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Publication number: 20100089831Abstract: The present invention relates to the preparation of chiral zeolites and their use in liquid chromatography. The zeolite material may be used in the chromatographic separation of mixtures of components, such as the separation of enantiomers.Type: ApplicationFiled: September 9, 2009Publication date: April 15, 2010Inventors: Mohd Refaei, James Salvador
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Patent number: 7683164Abstract: A compound which comprises a backbone having a plurality of chiral carbon atoms, the backbone bearing a plurality of ligands each being individually bound to a chiral carbon atom of the plurality of chiral carbon atoms, the ligands including one or more pair(s) of adjacent ligands each containing a moiety selected from the group consisting of a naturally occurring nucleobase and a nucleobase binding group, wherein moieties of the one or more pair(s) are directly linked to one another via a linker chain; building blocks for synthesizing the compound; and uses of the compound, particularly in antisense therapy.Type: GrantFiled: February 19, 2008Date of Patent: March 23, 2010Assignee: Bio-Rad Laboratories Inc.Inventor: David Segev
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Publication number: 20100068771Abstract: The invention relates to a process for the preparation of an enantiomerically enriched ?-amino alcohol, wherein glycine or a glycine salt and an aldehyde are reacted in the presence of a threonine aldolase and a decarboxylase to form the corresponding enantiomerically enriched ?-aminoalcohol, and wherein at least either the threonine aldolase or the decarboxylase is ?-selective. In a preferred embodiment of the invention at least either the threonine aldolase or the decarboxylase is enantioselective.Type: ApplicationFiled: April 12, 2007Publication date: March 18, 2010Inventors: Martin Schürmann, Daniel Mink, Michael Wolberg
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Patent number: 7674611Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: May 20, 2004Date of Patent: March 9, 2010Assignee: Mitsubishi Rayon Co., LtdInventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Patent number: 7670825Abstract: The present invention provides methods of producing an isoprenoid or an isoprenoid precursor in a genetically modified host cell. The methods generally involve modulating the level of hydroxymethylglutaryl-CoA (HMG-CoA) in the cell, such that the level of HMG-CoA is not toxic to the cell and/or does not substantially inhibit cell growth, but is maintained at a level that provides for high-level production of mevalonate, IPP, and other downstream products of an isoprenoid or isoprenoid pathway, e.g., polyprenyl diphosphates and isoprenoid compounds. The present invention further provides genetically modified host cells that are suitable for use in a subject method. The present invention further provides recombinant nucleic acid constructs for use in generating a subject genetically modified host cell, including recombinant nucleic acid constructs comprising nucleotide sequences encoding one or more mevalonate pathway enzymes, and recombinant vectors (e.g., recombinant expression vectors) comprising same.Type: GrantFiled: January 17, 2007Date of Patent: March 2, 2010Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Jack D. Newman, Douglas J. Pitera
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Publication number: 20100041110Abstract: R-hydroxynitrile lyases having an improved substrate acceptance, increased activity and increased selectivity, in which there is replacement in the amino acid sequence of R-hydroxynitrile lyases from the Rosaceae family either a) of the amino acid residue which corresponds to position 360 of the mature PaHNL5 protein by another apolar amino acid or a neutral amino acid and/or b) of the amino acid residue which corresponds to position 225 of the mature PaHNL5 protein by another polar amino acid, it also being possible where appropriate for 1 to 20 further residues in the active center or in the hydrophobic channel leading to the active center to be replaced.Type: ApplicationFiled: December 28, 2005Publication date: February 18, 2010Inventors: Roland Weis, Anton Glieder, Karl Gruber, Wolfgang Skrang, Oliver Maurer, Richard Gaisberger
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Patent number: 7659097Abstract: The present invention provides methods for a robust production of isoprenoids via one or more biosynthetic pathways. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. The invention also provides fermentation methods for high productivity of isoprenoids from genetically modified host cells.Type: GrantFiled: May 25, 2007Date of Patent: February 9, 2010Assignee: Amyris Biotechnologies, Inc.Inventors: Neil Stephen Renninger, Jack Newman, Keith Kinkead Reiling, Rika Regentin, Christopher John Paddon
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Patent number: 7642073Abstract: Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amine from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination of the target ketone under conditions sufficient to permit the formation of the desired amine to thereby produce the amine.Type: GrantFiled: April 9, 2007Date of Patent: January 5, 2010Assignee: Codexis, Inc.Inventor: J. David Rozzell, Jr.