Abstract: The present invention provides processes, methods, and systems for converting biomass-derived feedstocks to liquid fuels and chemicals. The method generally includes the reaction of a hydrolysate from a biomass deconstruction process with hydrogen and a catalyst to produce a reaction product comprising one of more oxygenated compounds. The process also includes reacting the reaction product with a condensation catalyst to produce C4+ compounds useful as fuels and chemicals.
Type:
Application
Filed:
March 17, 2014
Publication date:
September 18, 2014
Applicant:
Virent, Inc.
Inventors:
Andrew Held, Elizabeth Woods, Randy Cortright, Matthew Gray
Abstract: Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.
Abstract: Provided are isolated polypeptides having cellobiohydrolase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
Type:
Application
Filed:
November 15, 2012
Publication date:
September 4, 2014
Inventors:
Lan Tang, Junxin Duan, Yu Zhang, Ye Liu
Abstract: Process for the enzymatic synthesis of the compound of formula (I): Application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.
Type:
Application
Filed:
February 26, 2014
Publication date:
August 28, 2014
Applicant:
LES LABORATOIRES SERVIER
Inventors:
Sandrine Pedragosa-Moreau, François Lefoulon
Abstract: A method for producing pyruvic acid from polysaccharide alginic acid that is contained in large amounts in brown algae using the ability to assimilate alginic acid possessed by a lactate-dehydrogenase-gene-deficient Sphingomonas sp. A1 strain (ldh strain) is provided. The method for producing pyruvic acid comprises culturing the lactate-dehydrogenase-gene-deficient Sphingomonas sp. A1 strain (ldh strain) in a medium containing alginic acid, and causing the strain to produce pyruvic acid from alginic acid and then to secrete pyruvic acid into the medium.
Type:
Application
Filed:
February 25, 2014
Publication date:
August 28, 2014
Applicants:
MARUHA NICHIRO FOODS, INC., KYOTO UNIVERSITY
Inventors:
KOUSAKU MURATA, SHIGEYUKI KAWAI, NOBUYUKI SATO
Abstract: The invention relates to a method for preserving and/or storing microorganisms which exhibit at least one nitrile hydratase or nitrilase enzyme activity, with the preservation and/or storage being effected in an aqueous medium which comprises at least one aldehyde, with the total aldehyde concentration being in a range from 0.1 to 100 mM/l.
Type:
Grant
Filed:
December 24, 2003
Date of Patent:
August 26, 2014
Assignee:
BASF SE
Inventors:
Thomas Zelinski, Maria Keβeler, Bernhard Hauer
Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.
Type:
Grant
Filed:
November 13, 2013
Date of Patent:
August 26, 2014
Assignee:
E I du Pont de Nemours and Company
Inventors:
Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
Abstract: The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.
Type:
Application
Filed:
April 11, 2012
Publication date:
August 21, 2014
Applicant:
TEKNOLOGIAN TUTKIMUSKESKUS VTT
Inventors:
Antti Nyyssola, Hanna Kontkanen, Mari Hakkinen, Ville Pihlajaniemi, Markku Saloheimo, Johanna Buchert, Tiina Nakari-Setala
Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
August 10, 2012
Publication date:
August 21, 2014
Applicant:
Novozymes A/S
Inventors:
Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.
Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of two ends of a substituted or unsubstituted, linear or branched aliphatic hydrocarbons.
Type:
Application
Filed:
June 29, 2012
Publication date:
August 21, 2014
Applicant:
Novozymes A/S
Inventors:
Henrik Lund, Jesper Brask, Lisbeth Kalum, Ana Gutierrez Suarez, Esteban Daniel Babot, Rene Ullrich, Martin Hofrichter, Angel Tomas Martinez Ferrer, Jose Carlos Del Rio Andrade
Abstract: The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Grant
Filed:
October 22, 2012
Date of Patent:
August 19, 2014
Assignee:
E. I. du Pont de Nemours and Company
Inventors:
Robert Dicosimo, Mark Scott Payne, John Edward Gavagan
Abstract: Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved. In various embodiments, comprising other genetic modifications, including selected gene disruptions, 3-HP production is greater than in a control microorganism lacking such combinations of genetic modifications.
Type:
Grant
Filed:
September 27, 2010
Date of Patent:
August 19, 2014
Assignee:
OPX Biotechnologies, Inc.
Inventors:
Michael D Lynch, Christopher P. Mercogliano
Abstract: The present invention concerns a method for enhancing the growth rate, the biomass production and/or the fermentative potential of an anaerobic microorganism, comprising the steps consisting in: (a) modifying said anaerobic microorganism by inactivating the ohrR gene, the ohrR gene product or homolog thereof; (b) culturing the modified anaerobic microorganism obtained in step (a) under low-ORP anaerobic conditions.
Type:
Application
Filed:
June 5, 2012
Publication date:
August 14, 2014
Applicants:
Universite d'Avignon et des Pays de Vaucluse, Commissariat a l'energie atomique et aux ene alt
Inventors:
Jean Armengaud, Geremy Clair, Catherine Duport
Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.
Type:
Application
Filed:
July 9, 2012
Publication date:
July 31, 2014
Applicant:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
Inventors:
Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.
Abstract: Enzyme compositions including at least a) a cocktail of cellulases from Trichoderma reesei, and b) an enzymatic cocktail from Pycnoporus cinnabarinus including a cellobiose dehydrogenase (CDH) or c) a recombinant cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus, preferably also including a Family 61 glycoside hydrolase. Also, methods for degrading a lignocellulosic biomass, for producing a fermentation product, for producing gluconic acid, xylonic acid and/or xylobionic acid, for enhancing the production of gluconic acid, xylonic acid and/or xylobionic acid or for increasing the yield of sugars from a lignocellulosic biomass.
Type:
Application
Filed:
July 4, 2012
Publication date:
July 31, 2014
Applicants:
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, ARGO INDUSTRIES RECHERCHES ET DEVELOPPEMENTS, INSTITUT FRANCAIS DU PETROLE ENERGIE NOUVELLE
Inventors:
Jean-Claude Sigoillot, Jean-Guy Berrin, Mathieu Bey
Abstract: Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite.
Type:
Application
Filed:
September 7, 2012
Publication date:
July 24, 2014
Inventors:
Hui Xu, Ye Chen, Xin Li, Mark Stevens, Zhengfang Kang
Abstract: The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing.
Type:
Application
Filed:
January 22, 2014
Publication date:
July 24, 2014
Applicant:
Glycos Biotechnologies, Inc.
Inventors:
Daniel J. Monticello, Ryan Black, Paul Campbell
Abstract: The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.
Type:
Application
Filed:
January 31, 2013
Publication date:
July 24, 2014
Inventors:
Michael Koepke, Shilpa Nagaraju, Wendy Yiting Chen
Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate.
Type:
Application
Filed:
December 23, 2013
Publication date:
July 10, 2014
Applicant:
INVISTA North America S.á r.l.
Inventors:
Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
Abstract: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
Abstract: Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor.
Type:
Application
Filed:
March 4, 2014
Publication date:
July 10, 2014
Applicant:
Xyleco, Inc.
Inventors:
Marshall Medoff, Thomas Craig Masterman
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: In some variations, the invention provides a process for producing furfural, 5-hydroxymethylfurfural, and/or levulinic acid from cellulosic biomass, comprising: fractionating the feedstock in the presence of a solvent for lignin, sulfur dioxide, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; hydrolyzing the hemicellulose contained in the liquor, to produce hemicellulosic monomers; dehydrating the hemicellulose to convert at least a portion of C5 hemicelluloses to furfural and to convert at least a portion of C6 hemicelluloses to 5-hydroxymethylfurfural; converting at least some of the 5-hydroxymethylfurfural to levulinic acid and formic acid; and recovering at least one of the furfural, the 5-hydroxymethylfurfural, or the levulinic acid. Other embodiments provide a process for dehydrating hemicellulose to convert oligomeric C5 hemicelluloses to furfural and to convert oligomeric C6 hemicelluloses to 5-hydroxymethylfurfural.
Type:
Application
Filed:
November 18, 2013
Publication date:
July 3, 2014
Applicant:
API Intellectual Property Holdings, LLC
Inventors:
Theodora RETSINA, Vesa PYLKKANEN, Kimberly NELSON, Ryan O'CONNOR
Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
Type:
Application
Filed:
July 23, 2013
Publication date:
July 3, 2014
Applicant:
BP Corporation North Americca Inc.
Inventors:
David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
Abstract: A method of producing a sugar liquid with a cellulose-containing biomass as a raw material includes (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution, and (2) filtering the aqueous sugar solution obtain in (1) through an ultrafiltration membrane having a molecular weight cutoff of 600 to 2,000 to remove a fermentation inhibitor(s) into the permeate side and collect a sugar liquid from the feed side.
Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.
Type:
Application
Filed:
August 2, 2012
Publication date:
June 12, 2014
Inventors:
Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Rene Marcel De Jong
Abstract: A process for increasing the production of a glycolytic intermediate and/or an organic compound as defined herein by a cell that is able to express a nucleic acid molecule, wherein the expression of the nucleic acid molecule gives the cell the ability to increase the production of a glycolytic intermediate and/or the organic compound and wherein the carbon substrate is sugar and/or protein.
Abstract: A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.
Type:
Grant
Filed:
December 10, 2010
Date of Patent:
June 10, 2014
Assignee:
Veolia Water Solutions & Technologies Support
Inventors:
Alan Gideon Werker, Simon Olof Harald Bengtsson, Carl Anton Börje Karlsson
Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
Abstract: A method of biologically producing an aromatic carboxylic acid by contacting a substrate containing an aromatic carboxylic acid having a para-hydroxy group with a biocatalyst that removes the para-hydroxy group.
Type:
Application
Filed:
November 27, 2013
Publication date:
June 5, 2014
Applicant:
Samsung Electronics Co., Ltd.
Inventors:
Won Jae CHOI, Jin Ho AHN, Jong Won BYUN, Young Wan HA
Abstract: The present invention relates to a method of enzymatic hydrolysis of a lignocellulosic material, comprising the steps of: a) pretreating the lignocellulosic material to obtain a slurry having a pH of less than 6; b) adding NaOH, Ca(OH)2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60° C.; c) reducing the pH of the slurry to below 7; and optionally cooling the slurry from step b) to a temperature below 60° C.
Type:
Application
Filed:
June 11, 2012
Publication date:
June 5, 2014
Applicant:
Sekab E-Technology AB
Inventors:
Leif Jönsson, Björn Alriksson, Venkata Prabhakar Soudhama
Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproduc
Type:
Grant
Filed:
November 27, 2006
Date of Patent:
June 3, 2014
Assignee:
BASF SE
Inventors:
Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
Type:
Grant
Filed:
November 15, 2011
Date of Patent:
May 27, 2014
Assignee:
E I du Pont de Nemours and Company
Inventors:
Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
Type:
Application
Filed:
April 20, 2012
Publication date:
May 22, 2014
Applicant:
DSM IP ASSETS B.V.
Inventors:
Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve