Alpha-amylase, Microbial Source Patents (Class 435/202)
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Publication number: 20130203124Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: November 21, 2012Publication date: August 8, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Publication number: 20130203126Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: April 5, 2013Publication date: August 8, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Patent number: 8486682Abstract: To provide novel ?-amylase derived from a microorganism and a gene thereof. ?-amylase derived from Bacillus flexus is provided.Type: GrantFiled: April 21, 2009Date of Patent: July 16, 2013Assignee: Amano Enzyme Inc.Inventors: Akiko Matsunaga, Hitoshi Amano, Shotaro Yamaguchi
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Patent number: 8486390Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.Type: GrantFiled: July 31, 2012Date of Patent: July 16, 2013Assignee: Curemark LLCInventor: Joan M. Fallon
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Patent number: 8486681Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: January 27, 2009Date of Patent: July 16, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20130177964Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Nesterenkonia and related spp. are described.Type: ApplicationFiled: January 20, 2011Publication date: July 11, 2013Applicant: DANISCO US INC.Inventors: Brian E. Jones, Marc Kolkman
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Publication number: 20130178408Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: March 12, 2013Publication date: July 11, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Patent number: 8465957Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: GrantFiled: May 23, 2012Date of Patent: June 18, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20130149770Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.Type: ApplicationFiled: October 25, 2012Publication date: June 13, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Patent number: 8460916Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.Type: GrantFiled: July 2, 2012Date of Patent: June 11, 2013Assignee: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, Claudine Chang, Clement Choy, James T. Kellis, Jr., Brian E. Jones, Melodie Estabrook, Marc Koklman, Chris Leeflang, Casper Vroeman, Walter Weyler
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Patent number: 8455234Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.Type: GrantFiled: June 16, 2011Date of Patent: June 4, 2013Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, David A. Estell, Ronaldus W. J. Hommes, Brian E. Jones, Marc Kolkman, Chris Leeflang, Hiroshi Oh, Ayrookaran J. Poulose, Andrew Shaw, Wilhelmus A. H. van der Kley, Leonardus P. M. van Marrewijk
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Publication number: 20130122548Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: January 22, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Patent number: 8440444Abstract: The present invention relates to hybrid polypeptides having a first amino acid sequence having endo-amylase activity and a second amino acid sequence having carbohydrate binding activity. The present invention also relates to the use of the hybrid polypeptides in starch processing and baking.Type: GrantFiled: November 4, 2011Date of Patent: May 14, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Tina Spendler, Anders Viksoe Nielsen, Henrik Oestdal
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Patent number: 8426182Abstract: This invention relates to an alpha-amylase, a process for its preparation and the use of the amylase. The invention relates to a newly identified polynucleotide sequence from Alicyclobacillus pohliae comprising a gene that encodes the alpha-amylase enzyme. The invention features the full-length coding sequence of the gene as well as the amino acid sequence of the full-length functional protein of the gene. The invention also relates to methods of using these proteins in industrial processes, for example in food industry, such as the baking industry. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins and cells.Type: GrantFiled: July 23, 2012Date of Patent: April 23, 2013Assignee: DSM IP Assets B.V.Inventor: Lucie Parenicova
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Patent number: 8420370Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: January 20, 2012Date of Patent: April 16, 2013Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Publication number: 20130059315Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.Type: ApplicationFiled: October 25, 2012Publication date: March 7, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Patent number: 8383384Abstract: The present invention provides novel glycosyltransferase proteins produced by ascomycetous filamentous fungi (preferably species belonging to the genus Trichoderma, more preferably Trichoderma viride), as well as genes encoding the same. Among novel enzyme proteins provided by the present invention, particularly preferred is an enzyme protein obtained from the culture supernatant of Trichoderma viride strain IAM5141. The novel enzymes of the present invention allow glycosylation of flavonoid compounds to thereby improve their water solubility.Type: GrantFiled: June 22, 2012Date of Patent: February 26, 2013Assignee: Suntory Holdings LimitedInventors: Misa Ochiai, Harukazu Fukami, Masahiro Nakao, Akio Noguchi
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Patent number: 8361526Abstract: Dough with a high sucrose content (such as cake dough) tends to inhibit the activity of an anti-staling amylase such as Novamyl®, making it less effective to prevent the staling of dough-based products with high sucrose content such as cakes. A good anti-staling effect in cakes can be achieved by using a carefully selected anti-staling amylase with certain properties. Analysis of a 3D structure of Novamyl® shows that sucrose may inhibit by binding in the active site. Sucrose docks into the active site of Novamyl® differently from the substrate or inhibitor in published models 1QHO and 1QHP. This finding is used to design sucrose-tolerant variants.Type: GrantFiled: June 5, 2008Date of Patent: January 29, 2013Assignee: Novozymes A/SInventors: Lars Beier, Esben Peter Friis, Henrik Lundqvist, Peter Kamp Hansen, Tina Spendler
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Patent number: 8354256Abstract: Described are compositions and methods relating to the use of a glucoamylase in combination with a phytase in starch processing to reduce the levels of phytic acid in end-products.Type: GrantFiled: March 9, 2009Date of Patent: January 15, 2013Assignee: Danisco US Inc.Inventors: Suzanne Breneman, Oreste J. Lantero, Jr., Bradley A. Paulson, Jayarama K. Shetty
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Publication number: 20130011882Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: ApplicationFiled: May 15, 2012Publication date: January 10, 2013Applicant: DANISCO US INC.Inventors: LUIS G. CASCÃO-PEREIRA, JAMES T. KELLIS, JR., BRADLEY A. PAULSON, SCOTT D. POWER, SANDRA W. RAMER, VIVEK SHARMA, ANDREW SHAW, JAYARAMA K. SHETTY, DONALD E. WARD
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Patent number: 8334118Abstract: The invention relates to alpha amylases and to polynucleotides and polypeptides encoding the alpha amylases. In addition, methods of using the alpha amylases are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and at increased temperatures.Type: GrantFiled: September 25, 2009Date of Patent: December 18, 2012Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Jay M. Short, Eric J. Mathur, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska
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Patent number: 8323945Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.Type: GrantFiled: June 5, 2009Date of Patent: December 4, 2012Assignee: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
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Publication number: 20120301927Abstract: Variants of a Pseudomonas saccharophila G4-forming amylase (PS4) advantageously can catalyze high temperature saccharification to produce maltotetraose syrup from a starch liquefact or granular starch, e.g., derived from cornstarch. The PS4 variants are useful in a process of saccharification of starch that advantageously produces significant amounts of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.Type: ApplicationFiled: April 8, 2010Publication date: November 29, 2012Applicant: DANISCO US INC.Inventors: Gang Duan, Sung Ho Lee, Ying Qian, Rafael F. Sala, Jayarama Shetty
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Patent number: 8318158Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.Type: GrantFiled: October 12, 2011Date of Patent: November 27, 2012Assignee: Curemark, LLCInventor: Joan M. Fallon
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Patent number: 8318451Abstract: Pseudomonas saccharophila G4-forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.Type: GrantFiled: December 30, 2008Date of Patent: November 27, 2012Assignee: Danisco US Inc.Inventors: Andrew Shaw, Regina Chin, Karsten M. Kragh
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Patent number: 8318157Abstract: A maltogenic ?-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.Type: GrantFiled: March 12, 2008Date of Patent: November 27, 2012Assignee: Danisco US Inc.Inventors: Gang Duan, Kathy Qian, Martijn Scheffers, Jayarama K. Shetty, Pieter Van Solingen
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Publication number: 20120282658Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.Type: ApplicationFiled: January 4, 2011Publication date: November 8, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Carsten Andersen, Randall Deinhammer, Thomas Agersten Poulsen, Miguel Duarte Toscano, Peter Kamp Hansen, Henrik Friis-Madsen, Anders Viksoe-Nielsen, Signe Eskildsen Larsen, Lars L.H. Christensen
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Publication number: 20120282672Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: July 25, 2012Publication date: November 8, 2012Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Publication number: 20120252095Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: ApplicationFiled: May 23, 2012Publication date: October 4, 2012Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Sóeren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20120252086Abstract: The present invention provides a composition comprising a polypeptide comprising or consisting of an X46 domain and further a starch degrading enzyme and uses of such composition. The polypeptide comprising or consisting of an X46 domain can boost the activity of a starch-degrading enzyme.Type: ApplicationFiled: December 22, 2010Publication date: October 4, 2012Applicant: NOVOZYMES A/SInventors: Martin Borchert, Peter Fischer Hallin, Ming Li
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Publication number: 20120237981Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising a desired ingredient coated by a latex polymer coating.Type: ApplicationFiled: May 29, 2012Publication date: September 20, 2012Applicant: VERENIUM CORPORATIONInventors: WALTER CALLEN, TOBY RICHARDSON, GERHARD FREY, KEVIN GRAY, JANNE S. KEROVUO, MALGORZATA SLUPSKA, NELSON BARTON, EILEEN O'DONOGHUE, CARL MILLER
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Patent number: 8263382Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: GrantFiled: May 4, 2011Date of Patent: September 11, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Patent number: 8263368Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: June 29, 2009Date of Patent: September 11, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 8252573Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: February 3, 2011Date of Patent: August 28, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20120214196Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: August 23, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20120202265Abstract: The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variant thereof. AmyE or its variants thereof may be used to eliminate or reduce the iodine-positive starch presented in saccharide liquor. Also disclosed are a composition comprising an AmyE or variant thereof and a method utilizing an AmyE or variant thereof to eliminate or reduce the iodine-positive starch.Type: ApplicationFiled: October 19, 2010Publication date: August 9, 2012Inventors: Vivek Sharma, Jayarama Shetty, Bruce Strohm
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Patent number: 8236545Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.Type: GrantFiled: February 4, 2009Date of Patent: August 7, 2012Assignee: Danisco US Inc., Genencor DivisionInventors: Luis G. Cascao-Pereira, Claudine Chang, Clement Choy, James T. Kellis, Jr., Brian E Jones, Melodie Estabrook, Marc Kolkman, Chris Leeflang, Casper Vroemen, Walter Weyler
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Patent number: 8232086Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: January 20, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Patent number: 8222035Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.Type: GrantFiled: June 24, 2010Date of Patent: July 17, 2012Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska, Nelson R. Barton, Eileen O'Donoghue, Carl Miller
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Publication number: 20120172275Abstract: Compositions and methods relating to ?-amylase enzymes obtained from Bacillus megaterium strain DSM90, and to structurally related amylases, are described.Type: ApplicationFiled: February 24, 2010Publication date: July 5, 2012Applicant: Danisco US Inc.Inventors: Brian E. Jones, Marc Kolkman
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Publication number: 20120171731Abstract: The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.Type: ApplicationFiled: July 27, 2010Publication date: July 5, 2012Applicant: Danisco US Inc.Inventors: Glenn E. Nedwin, Vivek Sharma, Jayarama K. Shetty
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Patent number: 8206966Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: GrantFiled: November 3, 2008Date of Patent: June 26, 2012Assignee: Danisco US Inc.Inventors: Luis G. Cascāo-Pereira, James T. Kellis, Jr., Bradley A. Paulson, Scott D. Power, Sandra W. Ramer, Vivek Sharma, Andrew Shaw, Jayarama K. Shetty, Donald E. Ward
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Publication number: 20120142074Abstract: Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from Bacillus sp. no. 195, and methods of using the enzyme to clean surfaces and textiles. Also disclosed are variants of the enzyme with different signal sequences.Type: ApplicationFiled: December 6, 2011Publication date: June 7, 2012Applicant: Danisco US Inc.Inventors: Neelam S. Amin, Melodie Estabrook, Brian E. Jones, Marc Kolkman, Casper Vroemen, Walter Weyler
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Publication number: 20120122754Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: January 20, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Christel Thea Jørgensen, Henrik Bisgård-Frantzen, Allan Svendsen, Søren Kjærulff
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Patent number: 8178336Abstract: We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).Type: GrantFiled: December 19, 2008Date of Patent: May 15, 2012Assignee: Danisco A/SInventors: Patrick Maria Franciscus Derkx, Anja Kellet-Smith Hemmingen, Rie Mejldal, Bo Spange Sørensen, Karsten Matthias Kragh
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Patent number: 8153412Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.Type: GrantFiled: November 3, 2008Date of Patent: April 10, 2012Assignee: Danisco US Inc.Inventors: Claudine Y. Chang, Clement Choy, Melodie Estabrook, Mansi Goyal, Thomas P. Graycar, Victoria E. Huang, Brian E. Jones, Marc Kolkman, Karsten M. Kragh, Chris Leeflang, Scott D. Power, Sandra W. Ramer, Andrew Shaw, Casper Vroemen, Walter Weyler
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Patent number: 8143048Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.Type: GrantFiled: July 7, 2004Date of Patent: March 27, 2012Assignee: Danisco A/SInventors: Karsten Matthias Kragh, Kirsten Bojsen, Casper Tune Berg, Patrick M. F. Derkx, Anja H. Kellett-Smith, Charlotte Refdahl Thoudahl, Carol Fioresi, Wei Liu, Andrew Shaw, Gijsbert Gerritse
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Patent number: 8137944Abstract: Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.Type: GrantFiled: July 7, 2005Date of Patent: March 20, 2012Assignee: Danisco A/SInventors: Casper Tune Berg, Patrick M. F. Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
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Patent number: 8124395Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: November 23, 2010Date of Patent: February 28, 2012Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff