Alpha-amylase, Microbial Source Patents (Class 435/202)
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Patent number: 8119384Abstract: The present invention relates to processes for producing a starch hydrolyzate and optionally a fermentation product, such as ethanol.Type: GrantFiled: June 15, 2007Date of Patent: February 21, 2012Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Anders Viksoe-Nielsen, Jim Liu, Carsten Andersen
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Publication number: 20120040412Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.Type: ApplicationFiled: October 27, 2011Publication date: February 16, 2012Applicant: VERENIUM CORPORATIONInventors: Kevin A. Gray, Nahla Aboushadi, James B. Garrett
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Patent number: 8110380Abstract: A starch-based biodegradable material composition includes: an enzyme-hydrolyzed starch; and a biodegradable polyester selected from at least one of an aliphatic polyester of polybutylene succinate and an aliphatic-aromatic copolyester. The enzyme-hydrolyzed starch is prepared by hydrolyzing a native starch using a starch-hydrolyzing enzyme. The starch-hydrolyzing enzyme has an activity unit ranging from 15000 to 40000.Type: GrantFiled: April 29, 2008Date of Patent: February 7, 2012Assignee: Grace Biotech CorporationInventors: C. Will Chen, Ching-Huang Wang, Chin-Wei Chen, Yun-Ping Wang
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Publication number: 20120021489Abstract: Provided in part herein are compositions that include isolated microbial enzymes, such as amylases, lipases, and proteases that are useful as detergent additives. Also provided are methods of isolating amylases, lipases, and proteases from microbes, as well as methods of using these enzymes as detergent additives, and for stain removal.Type: ApplicationFiled: July 24, 2010Publication date: January 26, 2012Inventor: Shaon Ray Chaudhuri
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Patent number: 8084240Abstract: Described are variants of a parent ?-amylase that exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.Type: GrantFiled: June 2, 2009Date of Patent: December 27, 2011Assignee: Danisco US Inc.Inventors: William A Cuevas, Sang-Kyu Lee, Sandra W Ramer, Andrew Shaw, Amr R Toppozada, David E Estell, Sura H Hadi
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Patent number: 8084025Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.Type: GrantFiled: April 20, 2009Date of Patent: December 27, 2011Assignee: Curemark LLCInventor: Joan M. Fallon
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Patent number: 8080401Abstract: The present invention relates to ?-amylase variants that are stabilized to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, by point mutagenesis of asparagine (N) or glutamine (Q) residues located on the surface of the molecule to give other amino acid residues. The invention further relates to methods of increasing the stability of an ?-amylase to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, whereby at least one asparagine (N) or glutamine (Q) residue on the surface of the molecule is replaced with a different amino acid residue. The ?-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.Type: GrantFiled: March 28, 2007Date of Patent: December 20, 2011Assignee: Henkel AG & Co. KGaAInventors: Cornelius Bessler, Susanne Wieland, Karl-Heinz Maurer
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Patent number: 8076109Abstract: The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.Type: GrantFiled: April 4, 2011Date of Patent: December 13, 2011Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Eric Allain, Kevin S. Wenger, Henrik Bisgard-Frantzen
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Publication number: 20110275136Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.Type: ApplicationFiled: July 20, 2011Publication date: November 10, 2011Applicant: Novozymes A/SInventors: Carsten Andersen, Torben Vedel Borchert, Bjarne Rønfeldt Nielsen
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Patent number: 8039241Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 1 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: GrantFiled: April 6, 2010Date of Patent: October 18, 2011Assignee: I Novozymes A/SInventors: Lan Tang, Wenping Wu, Junxin Duan, Pia Francke Johannesen
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Publication number: 20110251117Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.Type: ApplicationFiled: June 16, 2011Publication date: October 13, 2011Applicant: Novozymes A/SInventors: Allan Svendsen, Henrik Bisgard-Frantzen, Torben Vedel Borchert
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Publication number: 20110244526Abstract: The present invention relates to an alpha-amylase blend, including a B. stearothermophilus alpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and a B. licheniformis alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.Type: ApplicationFiled: September 11, 2009Publication date: October 6, 2011Applicant: Danisco US Inc.Inventors: Bradley A. Paulson, Vivek Sharma, Jayarama K. Shetty
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Patent number: 8030050Abstract: The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.Type: GrantFiled: September 22, 2006Date of Patent: October 4, 2011Assignee: Danisco A/SInventors: Casper Tune Berg, Patrick Maria Franciscus Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
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Patent number: 8017351Abstract: The pharmaceutical use of amylases related to Bacillus alpha-amylases of SEQ ID NOs: 1-3, optionally in combination with a lipase and/or a protease. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The amylases of SEQ ID NOs: 1-3 are variants of amylases from Bacillus stearothermophilus, Bacillus licheniformis and Bacillus sp. The amylases of the invention have an improved efficacy in vivo, an improved pH-profile, a high specific activity, and/or an improved starch degradation profile.Type: GrantFiled: June 16, 2006Date of Patent: September 13, 2011Assignees: Novozymes A/S, Solvay Pharmaceuticals GmbHInventors: Allan Svendsen, Peter Collin Gregory
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Patent number: 8008241Abstract: An automatic dishwashing detergent composition comprising: a. at least 0.1 mg of active protease per gram of composition, wherein the protease is a variant of a protease that has at least 70% identity with the amino acid sequence of SEQ ID NO:1. wherein said variant comprise variations in one or more of the following positions: 32, 33, 48-54, 58-62, 94-107, 116, 123-133, 150, 152-156, 158-161, 164, 169, 175-186, 197, 198, 203-216 as compared with the protease in SEQ ID NO:1; and b. at least 0.05 mg of an active low temperature amylase per gram of composition.Type: GrantFiled: March 4, 2009Date of Patent: August 30, 2011Assignee: The Procter & Gamble CompanyInventor: Philip Frank Souter
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Publication number: 20110207201Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: May 4, 2011Publication date: August 25, 2011Applicant: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Publication number: 20110201082Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 25, 2011Publication date: August 18, 2011Applicant: Novozymes A/SInventors: Katja Salomon Johansen, Keith Gibson, Preben Nielsen, Helle Outtrup
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Patent number: 7993689Abstract: Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.Type: GrantFiled: November 11, 2009Date of Patent: August 9, 2011Assignee: Danisco US Inc.Inventors: Gang Duan, Fengi Li, Jayarama K. Shetty, Julius Vadakoot, Sridevi Vadakoot, legal representative
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Publication number: 20110177990Abstract: The present invention relates to variants of a parent ?-amylase, which parent ?-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: February 8, 2011Publication date: July 21, 2011Applicant: Novozymes A/SInventors: Henrik Bisgârd-Frantzen, Allan Svendsen, Torben Vedel Borchert
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Publication number: 20110171694Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: March 21, 2011Publication date: July 14, 2011Applicant: Novozymes A/SInventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
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Publication number: 20110165619Abstract: Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus Streptomyces provided that the microorganism contains a nucleic acid sequence which is not naturally present in it and which comprises at least the following sequence sections: a) nucleic acid sequence coding for a protein containing a cofactor, and b) a nucleic acid sequence which is at least 20% identical to the sequence given in SEQ ID NO. 1, or at least 20% identical to the sequence given in SEQ ID NO. 3, or a nucleic acid sequence which is structurally homologous to at least one of these sequences, wherein the amino acid sequence encoded by nucleic acid sequence b) functionally cooperates with the amino acid sequence encoded by nucleic acid sequence a) so that at least the amino acid sequence encoded by nucleic acid sequence a) is secreted by the microorganism.Type: ApplicationFiled: November 29, 2010Publication date: July 7, 2011Inventors: JOHANNES BONGAERTS, STEFAN EVERS, KERSTIN FOH, KARL-HEINZ MAURER
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Publication number: 20110159570Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: ApplicationFiled: January 20, 2011Publication date: June 30, 2011Applicant: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Patent number: 7960161Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+dependency and specific activity.Type: GrantFiled: September 27, 2007Date of Patent: June 14, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen
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Patent number: 7919681Abstract: The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.Type: GrantFiled: October 18, 2007Date of Patent: April 5, 2011Assignee: Syngenta Participations AGInventors: Michael B. Lanahan, Shib Sankar Basu, Christopher J. Batie, Wen Chen, Joyce Craig, Mark Kinkema
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Publication number: 20110076741Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: November 23, 2010Publication date: March 31, 2011Applicant: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Patent number: 7914993Abstract: The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.Type: GrantFiled: May 29, 2008Date of Patent: March 29, 2011Assignee: Syngenta Participations AG.Inventors: Christopher J. Batie, Greg Crabb, George W. Aux, Edward S. Cates, Jay Austin Dinwiddie, Aron Louis Silverstone, Rene Quadt, Carl A. Miller
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Patent number: 7915020Abstract: The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.Type: GrantFiled: August 29, 2008Date of Patent: March 29, 2011Assignee: Syngenta Participations AGInventors: Edward S. Cates, Jay Austin Dinwiddie, George Aux, Christopher Batie, Gregg Crabb
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Patent number: 7908094Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: March 30, 2009Date of Patent: March 15, 2011Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Publication number: 20110059491Abstract: To provide novel ?-amylase derived from a microorganism and a gene thereof. ?-amylase derived from Bacillus flexus is provided.Type: ApplicationFiled: April 21, 2009Publication date: March 10, 2011Applicant: AMANO ENZYME INC.Inventors: Akiko Matsunaga, Hitoshi Amano, Shotaro Yamaguchi
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Patent number: 7892806Abstract: The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.Type: GrantFiled: February 2, 2010Date of Patent: February 22, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Lars Beier, Jesper Vind, Tina Spendler, Morten Tovborg Jensen
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Publication number: 20110033575Abstract: Pseudomonas saccharophila G4-forming amylase (PS4) variants, and nucleic acids encoding these, and their uses in producing food products and in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch are provided.Type: ApplicationFiled: July 2, 2010Publication date: February 10, 2011Inventors: Karsten Matthias Kragh, Anja Hemmingsen Kellet-Smith, Andrew Shaw, Regina Chin
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Publication number: 20110033882Abstract: Variants of B. licheniformis alpha-amylase advantageously exhibit improved enzymatic performance. Suitable variants include those with an altered charge distribution on the surface of the enzyme or with altered active site residues. Structural modeling can inform the choice of amino acid modifications so that modified amino acids correspond to residues found in more active alpha amylases, for example. Compositions comprising the variants are useful in methods of cleaning surfaces, laundering textiles, desizing, treating starch, e.g., liquefaction and saccharification, and hydrolyzing biofilms off various substrates.Type: ApplicationFiled: May 28, 2008Publication date: February 10, 2011Inventors: Wolfgang Aehle, Neelam S. Amin
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Patent number: 7867746Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: November 2, 2007Date of Patent: January 11, 2011Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Patent number: 7858352Abstract: We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.Type: GrantFiled: December 8, 2006Date of Patent: December 28, 2010Assignee: Danisco A/SInventors: Karsten Matthias Kragh, Harm Mulder, Steffen Petersen, Helle Fomsgaard, Oene Robert Veltman
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Patent number: 7855322Abstract: The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.Type: GrantFiled: May 26, 2009Date of Patent: December 21, 2010Assignee: Syngenta Participations AGInventors: Michael B. Lanahan, Shib Sankar Basu, Christopher J Batie, Wen Chen, Joyce Craig, Mark Kinkema
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Patent number: 7833771Abstract: The present invention relates to polypeptides having alpha-amylase activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to a polypeptide having carbohydrate-binding affinity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: GrantFiled: December 5, 2007Date of Patent: November 16, 2010Assignees: Novozymes A/S, Novozymes North America IncInventors: Tine Hoff, Carsten Andersen, Tina Spendler, Sven Pedersen, Anders Vikso-Nielsen, Thomas Schafer, Jiyin Liu
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Patent number: 7833770Abstract: This is invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.Type: GrantFiled: September 27, 2006Date of Patent: November 16, 2010Assignee: Danisco A/SInventors: Karsten Matthias Kragh, Casper Tune Berg, Patrick M. F. Derkx, Anja H. Kellett-Smith, Charlotte Refdahl Thoudahl, Oene R. Veltman, Carol Fioresi, Gijsbert Gerritse, Wei Liu, Andrew Shaw
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Patent number: 7816108Abstract: The invention relates to uses of polypeptides having amylase activity, nucleic acids encoding them, and methods for making and using them. For example, in one aspect, the invention provides methods for making alcohol using polypeptides of the invention. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, for hydrolyzing a starch, comprising use of polypeptides of the invention, or polypeptides encoded by the nucleic acids of this invention.Type: GrantFiled: January 9, 2007Date of Patent: October 19, 2010Assignee: Syngenta Participations AGInventors: Walter Callen, Toby Richardson, Gerhard Frey, Jay M. Short, Eric J. Mathur, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska
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Publication number: 20100261232Abstract: Novel variant ?-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.2) are deleted. The disclosed variant ?-amylase enzymes show altered or improved stability and/or activity profiles.Type: ApplicationFiled: March 9, 2009Publication date: October 14, 2010Applicant: GENENCOR INTERNATIONAL, INC.Inventors: Eugenio Ferrari, Marc Kolkman, Craig E. Pilgrim
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Patent number: 7803598Abstract: An enzyme is described which enzyme is derived from family 13 of ?-amylases. The enzyme variant is obtainable by modifying a CGTase or a maltogenic ?-amylase. The enzyme is useful in preparing a food or a food product such as bakery products.Type: GrantFiled: September 12, 2005Date of Patent: September 28, 2010Assignee: Danisco A/SInventors: Karsten M. Kragh, Hans Leemhuis, Lubbert Dijkhuizen, Bauke W. Dijkstra
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Patent number: 7794997Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: GrantFiled: December 10, 2009Date of Patent: September 14, 2010Assignee: Novozymes A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Patent number: 7785855Abstract: The invention relates to nucleic acids encoding polypeptides having amylase activity, and methods for making and using them. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides encoded by the nucleic acids of this invention.Type: GrantFiled: January 9, 2007Date of Patent: August 31, 2010Assignee: Syngenta Participations AGInventors: Walter Callen, Toby Richardson, Gerhard Frey, Jay M. Short, Eric J. Mathur, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska
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Publication number: 20100215802Abstract: Dough with a high sucrose content (such as cake dough) tends to inhibit the activity of an anti-staling amylase such as Novamyl®, making it less effective to prevent the staling of dough-based products with high sucrose content such as cakes. A good anti-staling effect in cakes can be achieved by using a carefully selected anti-staling amylase with certain properties. Analysis of a 3D structure of Novamyl® shows that sucrose may inhibit by binding in the active site. Sucrose docks into the active site of Novamyl® differently from the substrate or inhibitor in published models 1QHO and 1QHP. This finding is used to design sucrose-tolerant variants.Type: ApplicationFiled: June 5, 2008Publication date: August 26, 2010Applicant: Novozymes A/SInventors: Lars Beier, Peter Esben Friis, Henrik Lundqvist, Peter Kamp Hansen, Tina Spendler
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Patent number: 7781201Abstract: This invention relates generally to polypeptides and peptides having an amylase activity, e.g., enzymes, polynucleotides encoding the enzymes, the use of such polypeptides and peptides. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides having amylase activity of the invention.Type: GrantFiled: January 9, 2007Date of Patent: August 24, 2010Assignee: Syngenta Participations AGInventors: Walter Callen, Toby Richardson, Gerhard Frey, Jay M. Short, Eric J. Mathur, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska
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Patent number: 7776576Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.Type: GrantFiled: July 7, 2004Date of Patent: August 17, 2010Assignee: Danisco A/SInventors: Karsten Matthias Kragh, Casper Tune Berg, Patrick M. F. Derkx, Anja H. Kellett-Smith, Charlotte Refdahl Thoudahl, Oene R. Veltman, Carol Fioresi, Wei Liu, Andrew Shaw, Gijsbert Gerritse
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Publication number: 20100190681Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: April 12, 2010Publication date: July 29, 2010Applicant: NOVOZYMES A/SInventors: THOMAS THISTED, SOREN KJAERULFF, CARSTEN ANDERSEN, CLAUS CRONE FUGLSANG
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Publication number: 20100190208Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.Type: ApplicationFiled: October 20, 2009Publication date: July 29, 2010Inventors: Kathleen A. CLARKSON, Nigel DUNN-COLEMAN, Suzanne E. LANTZ, Craig E. PILGRIM, Piet VAN SOLINGEN, Michael Ward
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Patent number: 7759093Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.Type: GrantFiled: March 8, 2004Date of Patent: July 20, 2010Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Kevin Gray, Janne S. Kerovuo, Malgorzata Slupska, Nelson Barton, Eileen O'Donoghue, Carl Miller
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Publication number: 20100143316Abstract: An isolated Bacillus amyloliquefaciens Ba-BPD1 having an Accession No. of DSM 21836 is provided. This novel strain has unique 16S ribosomal RNA sequenced as SEQ ID NO:1 and produces amylase, protease, cellulase and lipase, fibrinolytic enzyme to show their biodegradation capacities. Further, B. amyloliquefaciens Ba-BPD1 produces the antibiotic substances, such as iturin, fengycin and surfactin, and has antimicrobial capacity for inhibiting the fungal or bacterial growth. In conclusion, the novel strain of Bacillus amyloliquefaciens Ba-BPD1 and its products can be applied in agriculture, wastewater treatment, food industry and chemical industry.Type: ApplicationFiled: December 5, 2008Publication date: June 10, 2010Applicants: Council of Agriculture, Executive YuanInventors: Feng-Chia Hsieh, Suey-Sheng Kao
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Patent number: RE42046Abstract: The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25-40° C. to obtain a fermentation broth having 5 to 20% ethanol.Type: GrantFiled: October 23, 2008Date of Patent: January 18, 2011Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Oreste J. Lantero, Jr., Suzanne E. Lantz, Michael J. Pepsin, Jayarama K. Shetty