Alpha-amylase, Microbial Source Patents (Class 435/202)
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Publication number: 20100144575Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like ?-amylase, which variant has ?-amylase activity and at least one altered property as compared to the parent ?-amylase, comprises i) analysing the structure of the parent Termamyl-like ?-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like ?-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like ?-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like ?-amylase variant, which as compared to the parent Termamyl-like ?-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like ?-amylase variant with respect to the property in question.Type: ApplicationFiled: April 10, 2008Publication date: June 10, 2010Applicant: Novozymes A/SInventors: Allan Svendsen, Henrik Bisgard-Frantzen, Torben Vedel Borchert
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Patent number: 7727726Abstract: The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.Type: GrantFiled: August 29, 2008Date of Patent: June 1, 2010Assignee: Syngenta Participations AGInventors: Edward S. Cates, Jay Austin Dinwiddie, George Aux, Christopher Batie, Gregg Crabb
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Patent number: 7723079Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: GrantFiled: July 21, 2008Date of Patent: May 25, 2010Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward, III
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Patent number: 7713723Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: GrantFiled: December 22, 2009Date of Patent: May 11, 2010Assignee: Novozymes A/SInventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
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Publication number: 20100112637Abstract: The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: January 31, 2008Publication date: May 6, 2010Applicant: Novozymes A/SInventors: Martin Borchert, Anders Viksoe-Nielsen
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Publication number: 20100099597Abstract: The present invention relates to variants of a parent ?-amylase, which parent ?-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: July 28, 2008Publication date: April 22, 2010Applicant: NOVOZYMES A/SInventors: HENRIK BISGARD-FRANTZEN, ALLAN SVENDSEN, TORBEN VEDEL BORCHERT
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Publication number: 20100099161Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: December 22, 2009Publication date: April 22, 2010Applicant: Novozymes A/SInventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
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Publication number: 20100099598Abstract: The present invention relates to variants of a parent ?-amylase, which parent ?-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: July 28, 2008Publication date: April 22, 2010Applicant: NOVOZYMES A/SInventors: HENRIK BISGARD-FRANTZEN, ALLAN SVENDSEN, TORBEN VEDEL BORCHERT
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Publication number: 20100093034Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: ApplicationFiled: December 10, 2009Publication date: April 15, 2010Applicant: NOVOZYMES A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Publication number: 20100048446Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.Type: ApplicationFiled: February 4, 2009Publication date: February 25, 2010Applicant: DANISCO US INC., GENENCOR DIVISIONInventors: Luis G. Cascao-Pereira, Claudine Chang, Clement Choy, James T. Kellis, JR., Brian E. Jones, Melodie Estabrook, Marc Kolkman, Chris Leeflang, Casper Vroemen, Walter Weyler
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Patent number: 7666633Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.Type: GrantFiled: April 6, 2006Date of Patent: February 23, 2010Inventors: Walter Callen, Toby Richardson, Gerhard Frey, Carl Miller, Martin Kazaoka, Jay M. Short, Eric J. Mathur
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Patent number: 7659101Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: GrantFiled: December 15, 2005Date of Patent: February 9, 2010Assignee: Novozymes A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Publication number: 20100021587Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.Type: ApplicationFiled: November 3, 2008Publication date: January 28, 2010Applicant: Danisco US Inc., Genencor DivisionInventors: CLAUDINE Y. CHANG, CLEMENT CHOY, MELODIE ESTABROOK, MANSI GOYAL, THOMAS P. GRAYCAR, VICTORIA E. HUANG, BRIAN E. JONES, MARC KOLKMAN, KARSTEN M. KRAGH, CHRIS LEEFLANG, SCOTT D. POWER, SANDRA W. RAMER, ANDREW SHAW, CASPER VROEMEN, WALTER WEYLER
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Publication number: 20100015686Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.Type: ApplicationFiled: June 5, 2009Publication date: January 21, 2010Applicant: Danisco US Inc., Genencor DivisionInventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
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Patent number: 7638151Abstract: Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.Type: GrantFiled: March 10, 2004Date of Patent: December 29, 2009Assignee: Danisco US Inc.Inventors: Gang Duan, Feng Li, Jayarama K. Shetty, Julius Vadakoot, Sridevi Vadakoot, legal representative
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Publication number: 20090317864Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: ApplicationFiled: June 29, 2009Publication date: December 24, 2009Applicant: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Publication number: 20090314286Abstract: Described are variants of a parent ?-amylase that exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.Type: ApplicationFiled: June 2, 2009Publication date: December 24, 2009Applicant: Danisco US Inc., Genencor DivisionInventors: William A. Cuevas, Sang-Kyu Lee, Sandra W. Ramer, Andrew Shaw, Amr R. Toppozada, David E. Estell, Sura H. Hadi, Walter Weyler
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Patent number: 7635471Abstract: A xylanase gene, denoted xynR8, encoding a xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermostable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the ?-1,4-glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.Type: GrantFiled: August 21, 2006Date of Patent: December 22, 2009Assignee: Genozyme Biotech Inc.Inventors: Kou-Joan Cheng, Yo-Chia Chen, Hsueh-Ling Cheng, Shiou Hua Lin, Je Ruei Liu, Bi Yu
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Patent number: 7625737Abstract: The present invention relates to alpha-amylase variants having mutations corresponding to one or more of the following mutations A181E,D,Q,N,V; I201W,F,L; Q264R,D,K,E; Y290R,E,K; and S334A,V,T,L,I,D (using SEQ ID No. 2 for numbering).Type: GrantFiled: November 3, 2004Date of Patent: December 1, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Publication number: 20090286302Abstract: The present invention relates to processes for combined desizing and scouring of a sized fabric containing starch or starch derivatives during manufacture of fabric, which process comprises incubating said sized fabric in an aqueous treating solution having a pH in the range between 1 and 7, which aqueous treating solution comprises an acid amylase and at least one other acid enzyme facilitating said other fabric treatment steps. The present invention further relates to compositions used in said processes and the use of said compositions.Type: ApplicationFiled: June 6, 2007Publication date: November 19, 2009Applicant: Novosymes A/SInventors: Jiyin Liu, Sonja Salmon, Guifang Wu
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Patent number: 7601527Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an ?-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an ?-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an ?-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an ?-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like ?-amylase, which variant exhibits increased.Type: GrantFiled: January 19, 2005Date of Patent: October 13, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Sóeren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20090238923Abstract: Variants of B. licheniformis alpha-amylase exhibit improved enzymatic performance, including increased themostability and reduced calcium dependence. Compositions comprising the variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal. The nucleic acids encoding the variants are also disclosed.Type: ApplicationFiled: November 3, 2008Publication date: September 24, 2009Applicant: Danisco US Inc., Genencor DivisionInventors: Andrew SHAW, Sandra W. Ramer, Scott D. Power, Jayarama K. Shetty, Bradley A. Paulson, Vivek Sharma, Donald Ward
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Publication number: 20090239284Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: ApplicationFiled: December 15, 2005Publication date: September 24, 2009Applicant: Novozymes A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Publication number: 20090226564Abstract: The present invention relates variants of parent Bacillus amyloliquefaciens alpha-amylases, notably variants exhibiting altered pH-profile, which are advantageous with respect to applications of the variants in baking.Type: ApplicationFiled: June 29, 2007Publication date: September 10, 2009Applicant: Novozymes A/SInventors: Steffen Danielsen, Henrik Lundqvist
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Publication number: 20090226569Abstract: Described are variants of alpha (?)-amylases having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.Type: ApplicationFiled: February 4, 2009Publication date: September 10, 2009Applicant: DANISCO US INC., GENENCOR DIVISIONInventors: Sandra W. Ramer, Michael J. Pepsin, Andrew Shaw
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Publication number: 20090214706Abstract: We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.Type: ApplicationFiled: July 7, 2005Publication date: August 27, 2009Inventors: Casper Tune Berg, Patrick M.F. Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
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Patent number: 7579177Abstract: The present invention relates to processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of said granular starch followed by simultaneous saccharification and fermentation.Type: GrantFiled: May 28, 2004Date of Patent: August 25, 2009Assignee: Novozymes A/SInventors: Hans Sejr Olsen, Barrie Edmund Norman, Mogens Wumpelmann, Jeppe Wegener Tams
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Publication number: 20090203107Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: January 29, 2009Publication date: August 13, 2009Applicant: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
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Publication number: 20090203108Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: ApplicationFiled: March 30, 2009Publication date: August 13, 2009Applicant: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Publication number: 20090203109Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an ?-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an ?-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an ?-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an ?-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like ?-amylase, which variant exhibits increased.Type: ApplicationFiled: April 2, 2009Publication date: August 13, 2009Applicant: Novozymes A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20090202675Abstract: We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).Type: ApplicationFiled: December 19, 2008Publication date: August 13, 2009Inventors: Patrick Maria Franciscus Derkx, Anja Kellet-Smith Hemmingen, Rie Mejldal, Bo Spange Sorensen, Karsten Matthias Kragh
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Patent number: 7566561Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: December 23, 2002Date of Patent: July 28, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 7550281Abstract: Disclosed is a novel recombinant Bacillus bacterial strain constructed by genetic engineering which has high productivity of aipha-amylase. The Bacillus strain comprises an alpha-amylase gene inserted in the Bacillus chromosome under transcriptional control of a phage promoter. An efficient process for the rapid production of large amounts of alpha-amylase is also disclosed.Type: GrantFiled: August 7, 2002Date of Patent: June 23, 2009Assignee: The Hong Kong Polytechnic UniversityInventors: Yun Chung Leung, Wai Hung Lo, Jeffery Errington
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Publication number: 20090155882Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: November 2, 2007Publication date: June 18, 2009Applicant: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Publication number: 20090143270Abstract: The present invention relates to variants (mutants) of parent Termamyl-tike alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: January 27, 2009Publication date: June 4, 2009Applicant: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus von der Osten
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Patent number: 7541026Abstract: Disclosed are compositions comprising variants of a parent G. stearothermophilus alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: GrantFiled: November 3, 2008Date of Patent: June 2, 2009Assignee: Danisco US Inc., Genencor DivisionInventors: Scott D. Power, Sandra W. Ramer, Andrew Shaw
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Publication number: 20090117630Abstract: The present invention relates to processes for production of an ethanol product from granular starchType: ApplicationFiled: December 16, 2005Publication date: May 7, 2009Applicant: NOVOZYMES A/SInventors: Hans Sejr Olsen, Sven Pedersen
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Publication number: 20090117642Abstract: Disclosed are compositions comprising variants of a parent G. stearothermophilus alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: ApplicationFiled: November 3, 2008Publication date: May 7, 2009Inventors: Scott D. Power, Sandra W. Ramer, Andrew Shaw
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Publication number: 20090104681Abstract: The present invention relates to a polypeptide having alpha-amylase activity obtained from a strain of Aspergillus niger.Type: ApplicationFiled: February 17, 2006Publication date: April 23, 2009Inventors: Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Cherry Lin, Michael Ward
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Publication number: 20090082246Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.Type: ApplicationFiled: September 4, 2008Publication date: March 26, 2009Applicant: Novozymes A/SInventors: Carsten Andersen, Torben Vedel Borchert, Bjarne Ronfeldt Nielsen
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Patent number: 7498158Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: May 15, 2002Date of Patent: March 3, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20080299619Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: ApplicationFiled: July 21, 2008Publication date: December 4, 2008Applicant: GENENCOR INTERNATIONAL, INC.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward
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Publication number: 20080293607Abstract: Disclosed are variants of the ?-amylase derived from Bacillus sp. no. 707, compositions comprising said variants, compositions comprising the variants, and methods of using the variants. The methods of use include methods of cleaning surfaces, laundering textiles, desizing, hydrolyzing biofilms off various substrates, and treating starch (e.g., liquefaction and saccharification).Type: ApplicationFiled: March 4, 2008Publication date: November 27, 2008Inventors: Brian E. Jones, Casper Vroemen, Claudine Chang, Corey Naab, Hans De Nobel, Marc Kolkman, Walter Weyler
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Publication number: 20080292747Abstract: Described is a PS4 variant polypeptide derivable from a polypeptide having amylase activity selected from: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; referring to the numbering of a Pseudomonas saccharophilia exoamylase shown as SEQ ID NO: 1.Type: ApplicationFiled: January 7, 2008Publication date: November 27, 2008Inventors: Casper Tune Berg, Patrick Maria Franciscus Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sorensen, Charlotte Refdahl Thoudahl
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Publication number: 20080274531Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically the amylases of the current invention show an altered exospecifity.Type: ApplicationFiled: February 1, 2008Publication date: November 6, 2008Inventors: Casper Tune Berg, Kirsten Bojsen, Patrick M.F. Derkx, Carol Fioresi, Gijsbert Gerritse, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Charlotte Refdahl Thoudahl, Anja Hemmingsen Kellett-Smith
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Patent number: 7432099Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.Type: GrantFiled: August 9, 2001Date of Patent: October 7, 2008Assignee: Novozymes A/SInventors: Carsten Andersen, Torben Vedel Borchert, Bjarne Rønfeldt Nielsen
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Publication number: 20080227173Abstract: The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.Type: ApplicationFiled: September 22, 2006Publication date: September 18, 2008Inventors: Casper Tune BERG, Patrick Maria Franciscus Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sorensen, Charlotte Refdahl Thoudahl
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Patent number: 7407677Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and increased temperature.Type: GrantFiled: February 21, 2002Date of Patent: August 5, 2008Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Jay M. Short, Eric J. Mathur, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska
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Publication number: 20080171035Abstract: The purification and isolation of various genes which encode mammalian cell surface polypeptides. Nucleic acids, proteins, antibodies, and other reagents useful in modulating development of cells, e.g., lymphoid and myeloid, are provided, along with methods for their use.Type: ApplicationFiled: January 25, 2008Publication date: July 17, 2008Applicant: SCHERING CORPORATIONInventors: Alexander B.H. Bakker, Joseph H. Phillips, Lewis L. Lanier
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Publication number: 20080160573Abstract: Novel variant ?-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.2) are deleted. The disclosed variant ?-amylase enzymes show altered or improved stability and/or activity profiles.Type: ApplicationFiled: April 6, 2007Publication date: July 3, 2008Inventors: Eugenio Ferrari, Marc Kolkman, Craig E. Pilgrim