Lyase (4. ) Patents (Class 435/232)
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Publication number: 20110206685Abstract: Isolation of an iron regulated, nme-gene operon (designated sbn) from Staphylococcus aureus (RN6390), responsible for the biosynthesis of staphylobactm, a novel S. aureus siderophore Methods for treating or preventing a disease or condition caused by S. aureus infection, as well as methods for identifying agents that inhibit the biosynthesis of staphylobactm or inhibit the expression of genes in said sbn operon are further disclosed.Type: ApplicationFiled: September 8, 2005Publication date: August 25, 2011Inventors: David E. Heinrichs, Suzanne Dale
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Patent number: 8003356Abstract: Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.Type: GrantFiled: April 13, 2005Date of Patent: August 23, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Arie Ben-Bassat, Fateme Sima Sariaslani, Lisa L. Huang, Ranjan Patnaik, David J. Lowe
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Publication number: 20110201083Abstract: Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing higher alcohols including C5-C8 alcohol from a suitable substrate.Type: ApplicationFiled: April 18, 2011Publication date: August 18, 2011Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: James C. Liao, Kechun Zhang
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Publication number: 20110200576Abstract: Methods and composition related to the engineering of a novel protein with methionine-?-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.Type: ApplicationFiled: February 3, 2011Publication date: August 18, 2011Applicant: The Board of Regents of the University of Texas SystemInventors: George Georgiou, Everett Stone
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Publication number: 20110195478Abstract: The present invention discloses a bladder cancer biomarker and a test method using the same. The biomarker contains at least one of the mentioned 69 compounds, such as apolipoprotein A1 (APOA1), apolipoprotein A2 (APOA2), peroxiredoxin 2 (PRDX2), heparin cofactor 2 precursor (HCII), and serum amyloid A-4 protein (SAA4), which exist in the urine specimen of a testee. The expression intensity of the biomarker can facilitate diagnosis of bladder cancer and evaluation of aggressiveness and malignancy of bladder cancer. Thereby, the physician can arrange an optimized treatment to achieve the best therapeutic effect.Type: ApplicationFiled: August 4, 2010Publication date: August 11, 2011Inventors: Yi-Ting Chen, Jau-Song Yu, Chien-Lun Chen, Yu-Sun Chang
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Publication number: 20110189750Abstract: Disclosed is a formulation for the absorption of CO2, which comprises water, at least one CO2 absorption compound and a carbonic anhydrase as an activator to enhance the absorption capacity of the CO2 absorption compound. The invention also concerns the use of carbonic anhydrase, in a CO2 absorption solution to increase the CO2 absorption rate of such solution.Type: ApplicationFiled: March 31, 2011Publication date: August 4, 2011Applicant: CO2 SOLUTION INC.Inventors: Sylvie Fradette, Olivera Ceperkovic
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Patent number: 7985849Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.Type: GrantFiled: March 19, 2008Date of Patent: July 26, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
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Publication number: 20110177552Abstract: Methods and systems that include a method comprising: providing a system comprising one or more plastid proteins comprising a signal sequence and one or more chloroplast processing enzymes; and allowing at least one of the one or more chloroplast processing enzymes to cleave at least a portion of a signal sequence from at least one of the one or more plastid proteins.Type: ApplicationFiled: January 18, 2011Publication date: July 21, 2011Inventors: Caroline Virginia McNeil, Alyssa Baevich, Seiichi Paul Tillich Matsuda
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Patent number: 7977077Abstract: Enzymatic pathways for production of aminoshikimate, kanosamine, intermediates, and derivatives thereof; nucleic acid encoding and cells containing the enzymes; compositions containing aminoshikimate, kanosamine, an intermediate or derivative thereof; and use of the cells and pathways for biosynthetic production of aminoshikimate, kanosamine, intermediates, and derivatives thereof.Type: GrantFiled: January 30, 2007Date of Patent: July 12, 2011Assignee: Board of Trustees of Michigan State UniversityInventors: John W. Frost, Jiantao Guo
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Publication number: 20110159544Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: December 30, 2010Publication date: June 30, 2011Applicant: ROAL OyInventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
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Patent number: 7968089Abstract: The present disclosure relates to the preparation and deletion mutants of chondroitinase proteins and their use in methods for promoting the diffusion of therapeutic composition into tissues and their use for neurological functional recovery after central nervous system (“CNS”) injury or disease.Type: GrantFiled: July 3, 2008Date of Patent: June 28, 2011Assignee: Acorda Therapeutics, Inc.Inventors: Elliott A. Gruskin, Anthony O. Caggiano, Gargi Roy, Rohini D'Souza
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Patent number: 7959914Abstract: A method for modifying access of cells to extravascular spaces and regions comprising administering to a patient an enzyme that cleaves chondroitin sulfate proteoglycans is provided. It has been found that administration of an enzyme that cleaves chondroitin sulfate proteoglycans to a patient disrupts extravasation of cells from the blood stream into tissue. The present invention provides methods of reducing penetration of cells associated with inflammation into tissue of a patient. Several methods are also provided for the regulation and suppression of inflammation comprising administering enzymes that digest chondroitin sulfates. Also provided are methods of treating and preventing inflammation associated with infection, injury and disease.Type: GrantFiled: May 17, 2004Date of Patent: June 14, 2011Assignee: Acorda Therapeutics, Inc.Inventors: Elliott A. Gruskin, Jack L. Tseng, Anthony O. Caggiano
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Publication number: 20110124071Abstract: Provided are compositions and methods for producing hydrocarbons, including aldehydes, alkanes and alkenes. The hydrocarbons can be used in biofuels.Type: ApplicationFiled: November 17, 2010Publication date: May 26, 2011Applicant: LS9, IncInventors: Andreas Schirmer, Mathew Rude, Noah Helman
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Publication number: 20110118282Abstract: A compound having the structure useful for treatment or prevention of cardiovascular diseases, endothelial dysfunction, diastolic dysfunction, atherosclerosis, hypertension, angina pectoris, thromboses, restenoses, myocardial infarction, strokes, cardiac insufficiency, pulmonary hypertonia, erectile dysfunction, asthma bronchiale, chronic kidney insufficiency, diabetes, or cirrhosis of the liver in a human or animal patient.Type: ApplicationFiled: September 2, 2008Publication date: May 19, 2011Inventors: Amy R. Bittner, Christopher Joseph Sinz, Jiang Chang, Ronald M. Kim, J.W. Mirc, Emma R. Parmee, Qiang Tan
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Patent number: 7939301Abstract: The present invention is directed towards an asymmetric enzymic process for preparing optically active organic compounds. The process according to the invention is carried out in what are termed miniemulsions. The invention also relates to an enzymic reaction mixture which exhibits a miniemulsion.Type: GrantFiled: November 4, 2005Date of Patent: May 10, 2011Assignee: Evonik Degussa GmbHInventors: Harald Gröger, Karlheinz Drauz, Hendrik Hüsken, Katharina Landfester
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Patent number: 7939305Abstract: The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.Type: GrantFiled: April 8, 2005Date of Patent: May 10, 2011Assignee: Nutrinova Nutrition Specialties & Food Ingredients GmbHInventors: Markus Luy, Matthias Rüsing, Thomas Kiy
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Publication number: 20110104779Abstract: The present invention relates to use of heat-stable carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas or biogas. Furthermore, the invention relates to isolated polypeptides having carbonic anhydrase activity at elevated temperatures and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides.Type: ApplicationFiled: January 11, 2011Publication date: May 5, 2011Applicant: Novozymes A/SInventors: Martin Borchert, Paria Saunders
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Publication number: 20110092388Abstract: The invention relates to newly discovered nucleic acid molecules and proteins associated with breast cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human breast cancers are provided.Type: ApplicationFiled: November 23, 2010Publication date: April 21, 2011Applicants: MILLENNIUM PHARMACEUTICALS, INC., Board of Regents, The University of Texas systemInventors: JAMES LILLIE, Manjula Gannavarapu, Karen Glatt, Sebastian Hoersch, Shubhangi Kamatkar, Maureen Mertens Hattersley, John E. Monahan, Vickesh Myer, Youzhen Wang, Yong Yao Xu, Xumei Zhao, Rachel E. Meyers, Robert C. Bast, JR., Gabriel N. Hortobagyi, Lajos Pusztai
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Publication number: 20110085939Abstract: Engineered proteins are used in the assembly of two-dimensional and three-dimensional nanostructure assemblies, based on systematic design and production of protein node structures that can be interconnected, for example, with streptavidin or streptavidin-incorporating struts to produce structures with defined dimensions and geometry. Nanostructure assemblies having utility as functional devices or as resists for the patterning of substrates have architectures including polygons, polyhedra, two-dimensional lattices, and three-dimensional lattices.Type: ApplicationFiled: September 28, 2010Publication date: April 14, 2011Applicant: IMIPLEX LLCInventors: F. Raymond Salemme, Patricia C. Weber, Mark A. Rould
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Publication number: 20110088123Abstract: The invention provides isolated nucleic acid molecules which encode novel fatty acid dehydratase family members. The invention also provides recombinant expression vectors containing dehydratase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., SDA, EPA and DHA.Type: ApplicationFiled: June 2, 2009Publication date: April 14, 2011Applicants: BASF Plant Science GmbH, Bioriginal Food & Science Corp.Inventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20110086395Abstract: An isolated microorganism is provided which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate.Type: ApplicationFiled: September 2, 2010Publication date: April 14, 2011Inventors: Frank Wouter KOOPMAN, Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Johannes Hendrik De Winde
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Publication number: 20110086407Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.Type: ApplicationFiled: December 17, 2010Publication date: April 14, 2011Applicants: Novozymes A/S, Novozymes, Inc.Inventors: Randy Berka, Michael Rey, Preethi Ramaiya, Jens Tønne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
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Publication number: 20110081348Abstract: Method of identifying an anti-fungal agent which targets as an essential protein or gene of a fungus comprising contacting a candidate substance with (i) a protein which comprises the sequence shown by SEQ ID NOS: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 50, 53, 56, 59, 61 or 63, or (ii) a protein which has 60% identity with (i), or (iii) a protein comprising a fragment of (i) or (ii) which fragment has a length of at least 50 amino acids, or (iv) a polynucleotide that comprises a sequence which encodes (i), (ii) or (iii), or (v) a polynucleotide comprising a sequence which has at least 70% identity with the coding sequence of (iv), and determining whether the candidate substance binds or modulates (i), (ii), (iii), (iv), or (v), wherein binding or modulation of (i), (ii), (iii), (iv), or (v) indicates that the candidate substance is an anti-fungal agent.Type: ApplicationFiled: December 1, 2005Publication date: April 7, 2011Inventors: Nina Brogden, Michael John Bromley, Paul David Carr, Sarah Jane Kaye, Jason David Oliver, Daniel Scott Tuckwell
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Publication number: 20110076743Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: ApplicationFiled: April 22, 2010Publication date: March 31, 2011Inventors: Zachary Quinn Beck, Richard R. Bott, Christopher Lee Rife, Derek H. Wells, Jeffrey V. Miller
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Publication number: 20110076730Abstract: Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.Type: ApplicationFiled: July 19, 2007Publication date: March 31, 2011Applicant: Board of Trustees of Michigan State UniversityInventors: John W. Frost, Wei Niu
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Patent number: 7915026Abstract: Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.Type: GrantFiled: October 8, 2009Date of Patent: March 29, 2011Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Vincent J. J. Martin, Douglas J. Pitera, Seon-Won Kim, Sydnor T. Withers, III, Yasuo Yoshikuni, Jack Newman, Artem Valentinovich Khlebnikov
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Publication number: 20110059869Abstract: An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.Type: ApplicationFiled: February 27, 2009Publication date: March 10, 2011Inventors: Miki Kojima, Piero Carninci, Yoshihide Hayashizaki, Matthias Harbers
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Publication number: 20110052638Abstract: The invention relates to seven allergens of Aspergillus versicolor which are suitable for specific diagnostics of this mold which predominantly pollutes the interior. The invention is also directed to an immunological method of detecting a sensitization to A. versicolor, especially an allergy, wherein one or more of the above-mentioned seven allergens found are employed as diagnostic markers. The invention further relates to a pharmaceutical composition which includes one or more of these seven allergens as active substance, and to the use of one or more of these seven allergens for the desensitization to A. versicolor.Type: ApplicationFiled: July 3, 2008Publication date: March 3, 2011Applicant: HELMHOLTZ-ZENTRUM FÜR UMWELT-FORSCHUNG GMBHInventors: Andrea Müller, Dirk Benndorf, Katharina Bock, Martin Von Bergen, Olaf Herbarth
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Publication number: 20110053245Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-, arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.Type: ApplicationFiled: December 22, 2008Publication date: March 3, 2011Applicant: VERENIUM CORPORATIONInventors: David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
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Publication number: 20110045549Abstract: The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine in Corynebacterium glutamicum by way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of Corynebacterium glutamicum.Type: ApplicationFiled: May 3, 2010Publication date: February 24, 2011Inventors: Paul D. Hanke, Lhing-Yew Li-D'Elia, Holly J. Walsh, Corey M. Crafton, P. John Rayapati
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Publication number: 20110039314Abstract: A catalytic composition for the enzymatic conversion of nitriles to amides is disclosed. The composition contains a polymer gel and a nitrile hydratase (NHase). Also disclosed are methods of producing an amide from a nitrile using the catalytic composition.Type: ApplicationFiled: August 13, 2010Publication date: February 17, 2011Applicants: LOYOLA UNIVERSITY OF CHICAGO, The Trustees of Hamilton CollegeInventors: Richard C. Holz, Timothy Elgren
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Patent number: 7888081Abstract: Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.Type: GrantFiled: May 23, 2007Date of Patent: February 15, 2011Assignee: Cargill, IncorporatedInventors: Anil Bhagwan Khare, Brent H. Hilbert, Christopher Solheid, Sara C. McFarlan, Fernando A. Sanchez-Riera, Kent Floy, Paula M. Hicks
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Patent number: 7888072Abstract: The invention provides recombinant B. thetaiotaomicron GAG lyase polypeptides. The invention also provides nucleic acid molecules encoding such polypeptides, recombinant expression vectors containing B. thetaiotaomicron GAG lyase nucleic acid molecules, and host cells into which the expression vectors have been introduced. Characterization, diagnostic and therapeutic methods utilizing compositions of the invention are also provided.Type: GrantFiled: May 6, 2009Date of Patent: February 15, 2011Assignee: Momenta Pharmaceuticals, Inc.Inventor: James Myette
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Patent number: 7888095Abstract: The present invention provides methods of designing and generating polypeptide variants that have altered function compared to a parent polypeptide. The present invention further provides a computer program product for carrying out the design of a variant polypeptide. The present invention further provides nucleic acids encoding terpene cyclase variants, as well as vectors and host cells comprising the nucleic acids. The present invention further provides variant terpene cyclases; methods of producing the variant terpene cyclases; and methods of producing isoprenoid compounds.Type: GrantFiled: June 1, 2006Date of Patent: February 15, 2011Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Yasuo Yoshikuni
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Publication number: 20110033873Abstract: The present invention relates to proteomic markers for early detection of hepatocellular carcinoma, compositions for detecting changes of these proteomic markers, kits for detection of hepatocellular carcinoma, methods for detecting proteomic markers including these compositions, methods for screening drugs for hepatocellular carcinoma using these proteomic markers, and antibodies specific for these proteomic markers.Type: ApplicationFiled: March 12, 2009Publication date: February 10, 2011Inventors: Jin Young Park, Seok Joo Hong, Jongmin Kim, Youngtack Shim
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Publication number: 20110027860Abstract: Nucleic acid sequences coding for the chondroitinase ABC gene and isolated chondroitinase ABC protein produced in a host cell transformed with a nucleic acid vector directing the expression of a nucleotide sequence coding for chondroitinase ABC protein are described. Chondroitinase ABC prepared by chemical synthesis is also described. Monoclonal and polyclonal antibodies which are specifically reactive with chondroitinase ABC protein are disclosed. The isolated chondroitinase ABC can be used in methods of treating intervertebral disc displacement, promoting neurite regeneration, and detecting galactosaminoglycans.Type: ApplicationFiled: February 25, 2010Publication date: February 3, 2011Applicant: MARUHA NICHIRO SEAFOODS, INC.Inventors: Nobuyuki SATO, Masahiko SHIMADA, Hiroshi ODA
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Publication number: 20110027346Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: May 29, 2007Publication date: February 3, 2011Applicant: VERENIUM CORPORATIONInventors: David Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
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Publication number: 20110020938Abstract: An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an ?-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.Type: ApplicationFiled: November 26, 2008Publication date: January 27, 2011Applicant: AMANO ENZYME INC.Inventors: Takahumi Koyama, Yuzo Kojima, Kenji Kojima, Masashi Minoda
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Publication number: 20110014677Abstract: A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.Type: ApplicationFiled: July 9, 2008Publication date: January 20, 2011Inventors: Yuji Hatada, Yukari Ohta, Yuko Hidaka, Nobuyuki Nakamura
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Publication number: 20110014666Abstract: The present invention relates to a novel polypeptide having the enzymatic activity of conversion of methylglyoxal to lactic acid in a single step (known as glyoxalase III activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the glyoxalase III activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially 1,2-propanediol, acetol, and lactic acid by fermenting microorganisms wherein their glyoxalase III activity is modulated.Type: ApplicationFiled: March 16, 2009Publication date: January 20, 2011Applicant: Metabolic ExplorerInventors: Francois Voelker, Philippe Soucaille
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Publication number: 20110014158Abstract: The present invention relates to protein and nucleic acid mutants of chondroitinase ABCI. Such chondroitinase ABCI mutant enzymes exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: ApplicationFiled: May 17, 2010Publication date: January 20, 2011Inventors: Anthony O. CAGGIANO, Andrea VECCHIONE, Jennifer IACI
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Publication number: 20110009655Abstract: The present invention provides novel genes for ATP:citrate lyase. A nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 5, 6, 9 or 10 or a fragment thereof.Type: ApplicationFiled: October 24, 2008Publication date: January 13, 2011Applicant: SUNTORY HOLDINGS LIMITEDInventor: Misa Ochiai
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Publication number: 20110008867Abstract: The invention features compositions and methods that are useful for the production of a compound.Type: ApplicationFiled: December 22, 2009Publication date: January 13, 2011Applicant: GREENLIGHT BIOSCIENCESInventors: Andrey J. Zarur, James Swartz
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Publication number: 20110002906Abstract: The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.Type: ApplicationFiled: July 2, 2009Publication date: January 6, 2011Inventors: Harmeet Sidhu, Qingshan Li, Aaron Blake Cowley, Carl-Gustaf Golander
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Patent number: 7863029Abstract: A composition having an agent adapted to affect a multimeric protein by binding to a binding site of the multimeric protein and thereby affecting an equilibrium of units, wherein the multimeric protein has an assembly having a plurality of said units, wherein each of the units has a first complementary surface and a second complementary surface and wherein the first complementary surface of one unit is associated with the second complementary surface of another unit, provided that the assembly is at least one of different quaternary isoforms on a condition that in the multimeric protein (1) a structure of each of the units determines a structure of the different quaternary isoforms, (2) the units are in the equilibrium and (3) the structure of the different quaternary isoforms influences a function of the multimeric protein.Type: GrantFiled: April 21, 2008Date of Patent: January 4, 2011Assignee: Fox Chase Cancer CenterInventor: Eileen K. Jaffe
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Patent number: 7858355Abstract: The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.Type: GrantFiled: January 4, 2010Date of Patent: December 28, 2010Assignees: Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées de Toulouse, Centre National de la Recherche ScientifiqueInventors: Patricia Sarcabal, Christian Croux, Philippe Soucaille
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Publication number: 20100324258Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant micro-organism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: ApplicationFiled: February 20, 2009Publication date: December 23, 2010Applicant: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20100311132Abstract: The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.Type: ApplicationFiled: July 21, 2008Publication date: December 9, 2010Inventors: Maria Johanna Van Der Werf, Martinus Petrus Maria Caspers, Nicole Van Luijk, Peter Jan Punt
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Publication number: 20100311037Abstract: The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.Type: ApplicationFiled: November 28, 2008Publication date: December 9, 2010Applicant: Basf SEInventors: Bernhard Hauer, Nina Schneider, Krzysztof Okrasa, Jason Micklefield, David Leys, Colin Levy
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Patent number: 7842491Abstract: The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.Type: GrantFiled: November 8, 2004Date of Patent: November 30, 2010Inventors: Richard B. Thompson, Daniel Elbaum, Vincent L. Feliccia, David Christianson, Marcia W. Patchan, Zhengfang Ge, Badri P. Maliwal