Lyase (4. ) Patents (Class 435/232)
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Publication number: 20130205440Abstract: This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.Type: ApplicationFiled: February 1, 2013Publication date: August 8, 2013Applicant: DOW AGROSCIENCES LLCInventor: Dow AgroSciences LLC
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Publication number: 20130205441Abstract: This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.Type: ApplicationFiled: February 1, 2013Publication date: August 8, 2013Applicant: DOW AGROSCIENCES LLCInventor: Dow Agrosciences LLC
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Patent number: 8501452Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.Type: GrantFiled: June 25, 2008Date of Patent: August 6, 2013Assignee: Firmenich, SAInventors: Fredi Brühlmann, Laurent Fourage, Denis Wahler
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Patent number: 8501455Abstract: Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.Type: GrantFiled: May 9, 2012Date of Patent: August 6, 2013Assignee: Cargill, IncorporatedInventors: Ravi R. Gokarn, Olga V. Selifonova, Holly Jean Jessen, Steven John Gort, Thorsten Selmer, Wolfgang Buckel
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Publication number: 20130196359Abstract: Systems, compounds and methods for the conversion of C1 carbon compounds to higher carbon compounds useful for the generation of commodity compounds.Type: ApplicationFiled: September 15, 2011Publication date: August 1, 2013Applicant: University of Washington through its Center for CommercializationInventors: Justin Siegel, David Baker, Amanda Lee Smith, Mary E. Lidstrom, Catherine Louw
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Patent number: 8497102Abstract: The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. The present invention concerns a mutant methylglyoxal synthase (MGS) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the methylglyoxal synthase activity of the parent enzyme and the methylglyoxal synthase activity of the mutant MGS is not inhibited by orthophosphate as compared to the parent enzyme.Type: GrantFiled: July 30, 2010Date of Patent: July 30, 2013Assignee: Metabolic ExplorerInventors: François Voelker, Laurence Dumon-Seignovert, Philippe Soucaille
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Publication number: 20130189197Abstract: [PROBLEMS TO BE SOLVED] It is an object of the present invention to provide a gelator containing a long chain oxyaminopolyol capable of forming a gel with a small amount thereof over a liquid property range from acidic to alkaline, and a gel having high environmental suitability, biocompatibility and biodegradability. [MEANS FOR SOLVING THE PROBLEMS] A gelator, characterized by containing a long chain oxyaminopolyol of Formula (I): (where R1 is a C12-16 saturated aliphatic group or a C12-16 unsaturated aliphatic group having one double bond; R2 is a substituent which an amino acid has; and X is an oxygen atom or NH) and a pharmaceutically acceptable salt thereof; a self-assembly formed by the self-assembly of the gelator; and a gel containing the gelator or the self-assembly, and water, an aqueous solution, a hydrophilic organic solvent or a hydrophilic organic solution, or a hydrophobic organic solvent or a hydrophobic organic solution.Type: ApplicationFiled: March 12, 2013Publication date: July 25, 2013Applicant: NISSAN CHEMICAL INDUSTRIES, LTD.Inventor: NISSAN CHEMICAL INDUSTRIES, LTD.
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Publication number: 20130183737Abstract: The invention is based on the surprising finding that proteins regulated by excessive EGFR signalling in the liver may be used as biomarkers in the diagnosis, prognosis and/or monitoring of treatment of diseases, including liver cell dysplasia or hepatocellular carcinoma (HCC), wherein the protein is selected from a first group consisting of Arginase type II, 4931406C07Rik (Ester hydrolase C11orf54 homolog), Akr1c12 protein, Alanyl-tRNA synthetase, Aldo-keto reductase family 1 member C14, Aldo-keto reductase family 1 member C6, Aldolase 3, Alpha glucosidase 2, Beta 5-tubulin, Cai protein (Pdia4), cDNA sequence BC021917 (dihydroxyacetone kinase 2 homolog), Farnesyl diphosphate synthetase, Fatty acid binding protein 5 epidermal, Inosine triphosphatase, Interleukin 25, Kininogen 1, LIM and SH3 protein 1, Major vault protein, Nucb1 protein, Poly(rC) binding protein 2; heterogeneous nuclear ribonucleoprotein X, Psmd11 protein, RIKEN cDNA 2410004H02, Rps12 protein, Sars1 protein, Sorcin, T43799 proteasome protein pType: ApplicationFiled: June 23, 2011Publication date: July 18, 2013Applicant: MODPRO ABInventor: Jurgen Borlak
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Publication number: 20130171660Abstract: The present invention provides a blood marker for renal cancer, more specifically, a blood marker that can be practically used for clinical diagnosis of renal cancer. The present invention also provides a blood marker that can be practically used for follow-up after treatment such as surgery and during treatment such as medication for renal cancer. A blood marker for renal cancer selected from the group consisting of Galectin-1, Galectin-3, and ?-enolase. Galectin-1 and/or Galectin-3 as a blood marker for renal cancer for use in an examination performed before diagnostic imaging. ?-Enolase as a blood marker for renal cancer for use in monitoring during and/or after treatment for renal cancer.Type: ApplicationFiled: December 27, 2012Publication date: July 4, 2013Applicants: SHIMADZU CORPORATION, HYOGO COLLEGE OF MEDICINE, EDUCATIONAL CORPORATION MUKOGAWA GAKUINInventors: SHIMADZU CORPORATION, EDUCATIONAL CORPORATION MUKOGAWA GAKUIN, HYOGO COLLEGE OF MEDICINE
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Publication number: 20130171677Abstract: Novel fluorescent phycobiliprotein fusion proteins and methods of use are described. The novel phycobiliproteins can be produced in a cell comprising two or more heterodimeric lyases, an apoprotein and a bilin reductase, which components react to form the phycobiliprotein fusion protein. Also described are phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous, fluorescent, phycobiliprotein domain even in anoxic conditions.Type: ApplicationFiled: September 8, 2011Publication date: July 4, 2013Applicants: BOARD OF SUPERVISORS OF THE UNIVERSITY OF LOUISIANA SYSTEM, THE PENN STATE RESEARCH FOUNDATIONInventors: Donald A. Bryant, Wendy M. Schluchter, Richard M. Alvey, Avijit Biswas
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Publication number: 20130171718Abstract: The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also provided are polynucleotides encoding the carbonic anhydrase polypeptides and host cells capable of expressing them.Type: ApplicationFiled: December 20, 2012Publication date: July 4, 2013Applicant: CODEXIS, INC.Inventor: Codexis, Inc.
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Publication number: 20130171701Abstract: The present application, among others, relates to novel polypeptides having diterpene synthase activity, nucleic acid molecules encoding same, as well as to a gene cluster from Clitopilus passeckerianus which is thought to be involved in the biosynthetic pathway for producing pleuromutilin.Type: ApplicationFiled: March 9, 2011Publication date: July 4, 2013Inventors: Rudolf Mitterbauer, Thomas Specht
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Publication number: 20130157342Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: ApplicationFiled: June 26, 2012Publication date: June 20, 2013Applicant: Ajinomoto Co., Inc.Inventors: Masakazu SUGIYAMA, Kunihiko Watanabe, Shigeru Kawahara
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Publication number: 20130149752Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.Type: ApplicationFiled: November 19, 2012Publication date: June 13, 2013Applicant: EVOCATAL GMBHInventor: EVOCATAL GMBH
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Publication number: 20130149771Abstract: The present invention relates to use of Persephonella carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas, biogas or ambient air. The Persephonella carbonic anhydrases are especially well suited for these purpose due to their extreme thermostability.Type: ApplicationFiled: August 24, 2011Publication date: June 13, 2013Applicant: NOVOZYMES A/SInventor: Martin Simon Borchert
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Publication number: 20130143291Abstract: The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.Type: ApplicationFiled: March 30, 2011Publication date: June 6, 2013Applicant: Codexis, Inc.Inventors: Robert McDaniel, Galit Meshulam-Simon, Oscar Alvizo, Xiyun Zhang
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Patent number: 8455239Abstract: The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: June 22, 2010Date of Patent: June 4, 2013Assignee: Gevo, Inc.Inventors: Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano, Peter Meinhold, Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith
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Publication number: 20130137142Abstract: Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. In other exemplary embodiments, methods and materials are disclosed for degrading mannans.Type: ApplicationFiled: October 24, 2012Publication date: May 30, 2013Applicant: University of Central Florida Research Foundation, Inc.Inventor: University of Central Florida Research Foundation
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Publication number: 20130133102Abstract: A conserved region of sequence in bacterial microcompartment (BMC) enzymes and proteins was identified. Peptide sequences derived from this conserved region of native BMC proteins and enzymes appear to target the hexameric facets of BMC shell proteins. These peptides were predicted to share general properties of a predicted alpha helical conformation, flanked by poorly conserved segment(s) of primary structure); for each type of encapsulated protein, and for each functionally distinct BMC. These peptides can be used as targeting signals for integrating biomolecules and molecules into bacterial microcompartments or for attaching molecules or biomolecules to native or non-native bacterial microcompartment shell proteins.Type: ApplicationFiled: August 1, 2012Publication date: May 23, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Cheryl A. Kerfeld, James N. Kinney
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Patent number: 8445659Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.Type: GrantFiled: August 16, 2010Date of Patent: May 21, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
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Publication number: 20130122548Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: January 22, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Patent number: 8440436Abstract: The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.Type: GrantFiled: July 21, 2008Date of Patent: May 14, 2013Assignee: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNOInventors: Maria Johanna Van Der Werf, Martinus Petrus Maria Caspers, Nicole Van Luijk, Peter Jan Punt
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Publication number: 20130108607Abstract: The subject invention relates in one aspect to an oxalate degrading composition, which includes at least one oxalate degrading enzyme. The composition includes an enriched insoluble component of fungal biosample, and the composition is effective to degrade oxalate at a pH of 1.9 or higher. The composition is protected from protease degradation such as pepsin, trypsin and chymotrypsin. The composition is capable of withstanding the conditions of the stomach, small intestines, and/or large intestines of a subject.Type: ApplicationFiled: November 23, 2010Publication date: May 2, 2013Inventors: Aaron Cowley, Qing-Shan Li
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Patent number: 8431122Abstract: The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.Type: GrantFiled: July 2, 2009Date of Patent: April 30, 2013Assignee: Oxthera Intellectual Property ABInventors: Harmeet Sidhu, Qingshan Li, Aaron Blake Cowley, Carl-Gustaf Golander
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Patent number: 8431381Abstract: The present invention provides novel genes for ATP:citrate lyase. A nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 5, 6, 9 or 10 or a fragment thereof.Type: GrantFiled: October 24, 2008Date of Patent: April 30, 2013Assignee: Suntory Holdings LimitedInventor: Misa Ochiai
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Publication number: 20130101565Abstract: The present invention falls within the field of biomedicine. Specifically, the present invention relates to the use of carbonic anhydrase II for the manufacture of a medicament for the prevention and/or treatment of the damage caused by ischemia, ischemia followed by reperfusion or toxins, acute failure or rejection of a transplanted organ, preferably the kidney. In a preferred embodiment, the toxin is cisplatin.Type: ApplicationFiled: July 12, 2010Publication date: April 25, 2013Applicant: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)Inventors: Georgina Hotter Corripio, José Luis Viñas, Anna Sola Martínez
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Patent number: 8420371Abstract: A crystal comprising an isoform of an N-terminally truncated glutamic acid decarboxylase (GAD) chosen from the group consisting of a monoclinic P21 space group with unit cell dimensions of a =84.05±2.3 ?, b=62.74±2.3 ?, c=101.35±2.3 ? and ? =106.69° (GAD67) or an orthorhombic C2221 space group with unit cell dimensions of a=78.25±2.3 ?, b=99.05±2.3 ? and c=120.01±2.3 ?(GAD65).Type: GrantFiled: September 13, 2007Date of Patent: April 16, 2013Assignee: Monash UniversityInventors: James Charles Whisstock, Ashley Maurice Buckle, Gustavo Fenalti, Ruby Hong Ping Law, Merrill Joy Rowley
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Patent number: 8420364Abstract: The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also provided are polynucleotides encoding the carbonic anhydrase polypeptides and host cells capable of expressing them.Type: GrantFiled: June 30, 2011Date of Patent: April 16, 2013Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Mike Benoit, Scott Novick
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Publication number: 20130089535Abstract: Disclosed herein is a novel enzymatic agent effective in reducing acetaldehyde in the oral cavity. It has been found that an aldehyde dehydrogenase derived from a microorganism belonging to the genus Saccharomyces and a threonine aldolase derived from Escherichia coli are effective in reducing low concentrations of acetaldehyde. Therefore, an agent for reducing acetaldehyde in the oral cavity is provided, which contains these enzymes as active ingredients.Type: ApplicationFiled: June 7, 2011Publication date: April 11, 2013Applicant: AMANO ENZYME INC.Inventors: Kan Yamashiro, Takahumi Koyama
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Patent number: 8409841Abstract: An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.Type: GrantFiled: August 16, 2011Date of Patent: April 2, 2013Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Yasuhisa Asano, Takanori Akiyama, Fujio Yu, Eiji Sato
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Patent number: 8404232Abstract: The present invention relates to protein and nucleic acid mutants of chondroitinase ABCI. Such chondroitinase ABCI mutant enzymes exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: GrantFiled: May 17, 2010Date of Patent: March 26, 2013Assignee: Acorda Therapeutics, Inc.Inventors: Anthony O. Caggiano, Andrea Vecchione, Jennifer Iaci
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Publication number: 20130071877Abstract: Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.Type: ApplicationFiled: October 26, 2012Publication date: March 21, 2013Inventors: Joseph Chappell, Kyoungwhan Back
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Publication number: 20130071898Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.Type: ApplicationFiled: March 23, 2012Publication date: March 21, 2013Applicant: Butamax(TM) Advanced Biofuels LLCInventors: LARRY CAMERON ANTHONY, Hongxian He, Lixuan Lisa Huang, Daniel P. Okeefe, Arthur Leo Kruckeberg, Yougen Li, Lori Ann Maggio-Hall, Jessica Mcelvain, Mark J. Nelson, Ranjan Patnaik, Steven Cary Rothman
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Patent number: 8398989Abstract: Human cystathionine ?-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine ?-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine ?-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.Type: GrantFiled: August 30, 2011Date of Patent: March 19, 2013Assignee: The Regents of the University of ColoradoInventors: Jan P. Kraus, Jana Oliveriusova
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Patent number: 8399227Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.Type: GrantFiled: September 23, 2011Date of Patent: March 19, 2013Assignee: Joule Unlimited Technologies, Inc.Inventors: David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Christian Perry Ridley, Dan Eric Robertson, Regina Wilpiszeski, Martha Sholl
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Publication number: 20130067619Abstract: Nucleic acid molecules encoding polypeptides having polyketide synthase activity have been identified and characterized. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.Type: ApplicationFiled: April 15, 2011Publication date: March 14, 2013Inventors: Jonathan E. Page, Steve Gagne
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Publication number: 20130059331Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.Type: ApplicationFiled: April 19, 2011Publication date: March 7, 2013Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
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Patent number: 8383389Abstract: An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.Type: GrantFiled: August 16, 2011Date of Patent: February 26, 2013Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Yasuhisa Asano, Takanori Akiyama, Fujio Yu, Eiji Sato
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Publication number: 20130045891Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.Type: ApplicationFiled: October 27, 2011Publication date: February 21, 2013Inventors: ZACHARY Q. BECK, DAVID A. ESTELL, JEFFREY V. MILLER, CHRISTOPHER L. RIFE, DEREK H. WELLS
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Publication number: 20130045506Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 13, 2012Publication date: February 21, 2013Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Publication number: 20130039898Abstract: Provided herein are phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and/or a reduced immunogenicity as compared to a wild-type PAL. Further provided are compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, formulation, and use of such compositions for industrial and therapeutic purposes, e.g., treating hyperphenylalaninemia, including phenylketonuria, and other disorders, including cancer.Type: ApplicationFiled: February 3, 2011Publication date: February 14, 2013Applicant: BIOMARIN PHARMACEUTICAL INC.Inventors: Augustus O. Okhamafe, Sean M. Bell, G. Nick Zecherle, Kris Antonsen, Yanhong Zhang, Kieu Ly Tran, Paul A. Fitzpatrick, Emil D. Kakkis, Michel Claude Vellard, Daniel J. Wendt, Mubarack Muthalif
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Publication number: 20130040322Abstract: Provided are methods and compositions for determining whether an individual has Sjögren's disease (SD). The method entails determining in a biological sample from the individual the presence of antibodies directed to salivary gland protein 1 (SP-I), parotid secretory protein (PSP), carbonic anhydrase 6 (C A6), or determining a combination of the antibodies. Determining that the individual has SD is based on the presence of the antibodies. The method provides for detection of early SD. Kits for antibody detection containing the antigens to which the antibodies of SD patients are directed are also provided.Type: ApplicationFiled: January 21, 2011Publication date: February 14, 2013Inventors: Julian L. Ambrus, Long Shen
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Publication number: 20130040297Abstract: The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a) Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b) Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c) Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d) Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e) Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f) Using the information of the SNP's in rational design of a cell capable of converting arabinose; g) Construction of the cell capable of converting arabinose designed in step f).Type: ApplicationFiled: April 19, 2011Publication date: February 14, 2013Applicant: DSM IP ASSETSInventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Wilbert Herman Marie Heijne, Gijsberdina Pieternella Van Suylekom
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Patent number: 8372621Abstract: Novel enzymes and novel enzymatic pathways for the pyruvate-based synthesis of shikimate or at least one intermediate thereto or derivative thereof, nucleic acids encoding the enzymes, cells transformed therewith, and kits containing said enzymes, cells, or nucleic acid. A KDPGal aldolase is used to perform condensation of pyruvate with D-erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP); a 3-dehydroquinate synthase is used to convert the DAHP to 3-dehydroquinate (DHQ); DHQ dehydratase can then convert DHQ to the key shikimate intermediate, 3-dehydroshikimate.Type: GrantFiled: July 20, 2010Date of Patent: February 12, 2013Assignee: Board of Trustees Operating Michigan State UniversityInventor: John W. Frost
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Publication number: 20130035232Abstract: Provided herein are methods for inducing and stabilizing an enzyme activity. Optionally, the enzyme is in a microorganism capable of producing the enzyme. In some embodiments, the enzyme can be nitrile hydratase, amidase, or asparaginase I. Provided are compositions comprising enzymes or microorganisms having induced and/or stabilized activity. Also provided are methods of delaying a plant development process by exposing a plant or plant part to the enzymes or microorganisms having induced and/or stabilized activity.Type: ApplicationFiled: January 24, 2011Publication date: February 7, 2013Applicant: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.Inventors: George E. Pierce, Trudy Ann Tucker
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Publication number: 20130017583Abstract: The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression.Type: ApplicationFiled: November 10, 2010Publication date: January 17, 2013Applicant: Massachusetts Institute of TechnologyInventors: Charles Forrester Budde, Chokyun Rha
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Publication number: 20130017204Abstract: The invention relates to the polynucleotide sequence of a nontypeable stain of Haemophilus influenzae (NTHi) and polypeptides encoded by the polynucleotides and uses thereof. The invention also relates to NTHi genes which are upregulated during or in response to NTHi infection of the middle ear and/or the nasopharynx.Type: ApplicationFiled: July 19, 2012Publication date: January 17, 2013Applicants: THE BOARD OF REGENTS OF UNIVERSITY OF OKLAHOMA, NATIONWIDE CHILDREN'S HOSPITAL, INC.Inventors: Lauren O. Bakaletz, Robert S. Munson, JR., David W. Dyer
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Publication number: 20130019344Abstract: The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.Type: ApplicationFiled: September 18, 2012Publication date: January 17, 2013Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: Pioneer Hi-Bred International, Inc.
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Publication number: 20130017608Abstract: This invention relates to a novel Pleuromutilin gene cluster and methods of increasing yields of Pleuromutilin produced by Clitopilus and related basidiomycete species.Type: ApplicationFiled: October 28, 2010Publication date: January 17, 2013Applicant: Glaxo Wellcome HouseInventors: Andrew Mark Bailey, Gatherine Collins, Amanda Crawford, Gary Foster, Alison Michelle Griffin, Sreedhar Kilaru, Angela Scrogham, David Spence
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Patent number: 8354261Abstract: The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also provided are polynucleotides encoding the carbonic anhydrase polypeptides and host cells capable of expressing them.Type: GrantFiled: June 30, 2011Date of Patent: January 15, 2013Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Mike Benoit, Irene Fusman, Jack Liang, Luan Nguyen, Scott Novick