Lyase (4. ) Patents (Class 435/232)
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Patent number: 8697416Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: GrantFiled: June 26, 2012Date of Patent: April 15, 2014Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Shigeru Kawahara
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Patent number: 8691536Abstract: To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity.Type: GrantFiled: July 15, 2010Date of Patent: April 8, 2014Assignees: Bio-I Co., Ltd.Inventors: Yoshihisa Nakano, Mitsuhiro Uenoyama, Erina Kobayashi
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Publication number: 20140093536Abstract: The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of a protein isolated from bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.Type: ApplicationFiled: July 30, 2013Publication date: April 3, 2014Inventors: Jiri Pillich, John C. Balcarek
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Patent number: 8679481Abstract: A method for modifying access of cells to extravascular spaces and regions comprising administering to a patient an enzyme that cleaves chondroitin sulfate proteoglycans is provided. It has been found that administration of an enzyme that cleaves chondroitin sulfate proteoglycans to a patient disrupts extravasation of cells from the blood stream into tissue. The present invention provides methods of reducing penetration of cells associated with inflammation into tissue of a patient. Several methods are also provided for the regulation and suppression of inflammation comprising administering enzymes that digest chondroitin sulfates. Also provided are methods of treating and preventing inflammation associated with infection, injury and disease.Type: GrantFiled: May 9, 2011Date of Patent: March 25, 2014Assignee: Acorda Therapeutics, Inc.Inventors: Elliott A. Gruskin, Jack L. Tseng, Anthony O. Caggiano
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Publication number: 20140082771Abstract: The present invention provides substantially purified or isolated fungi of Nodulisporium spp. or Ascocoryne spp., plants infected with said fungi, organic compounds produced by said fungi, and related nucleic acids, polypeptides and methods.Type: ApplicationFiled: May 22, 2012Publication date: March 20, 2014Inventors: German Carlos Spangenberg, Timothy Ivor Sawbridge, Simone Jane Rochfort, Scott W. Mattner, Ross C. Mann
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Publication number: 20140075600Abstract: The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.Type: ApplicationFiled: December 15, 2010Publication date: March 13, 2014Applicant: Isobionics B.V.Inventors: Jihane Achkar, Theodorus Sonke, Martinus Julius Beekwilder, Hendrik Jan Bouwmeester, Hendrik Jan Bosch
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Publication number: 20140073020Abstract: Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (?)-ambroxide.Type: ApplicationFiled: July 30, 2013Publication date: March 13, 2014Applicant: Allylix, Inc.Inventors: Grace Eunyoung Park, Bryan N. Julien, Richard Burlingame
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Patent number: 8669089Abstract: The present invention notably relates to ulvan lyases, to nucleic acid sequences coding for these ulvan lyases, to vectors comprising these coding sequences, to a method of manufacturing these ulvan lyases, as well as to a method of degrading ulvans using these ulvan lyases and applicable applications to the degradation products of the ulvans. The ulvan lyases of the present invention, or ulvanolytic protein, are notably defined as proteins of 30 or 46 kD comprising the following four sequences in their peptide sequence: PNDPNLK, LLEVGNTGTFGSTGS, DLANPDNV and WNLPE.Type: GrantFiled: June 16, 2011Date of Patent: March 11, 2014Assignees: Centre National de la Recherche Scientifique-CNRS, Centre d'Etude et de Valorisation des Algues-CEVA, Universite Pierre et Marie CurieInventors: William Helbert, Pi Nyvall-Collen, Yannick Lerat, Jean-François Sassi
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Publication number: 20140065686Abstract: Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.Type: ApplicationFiled: August 2, 2013Publication date: March 6, 2014Applicant: Scientist of Fortune, S.A.Inventor: Phillippe Marliere
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Publication number: 20140057251Abstract: Using the efficiency of next generation sequencing, a draft de novo reference sequence for the Cannabis (C.) Sativa and C. Indica genomes has been generated as well as four full length contiguous sequences with homology to THCA and CBDA synthases and 10 partially homologous contigs with truncated ORFs. In particular aspects the invention is directed to an (one or more) isolated sequence (e.g., nucleic acid sequence, DNA, RNA, genomic sequence, polypeptide) of a Cannabis genome and uses thereof.Type: ApplicationFiled: August 17, 2012Publication date: February 27, 2014Applicant: Medicinal Genomics CorporationInventor: Kevin McKernan
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Patent number: 8658393Abstract: Demethylation of a methylated DNA sequence in a eukaryotic cell is described, utilizing a molecule that includes at least a first domain that exhibits a cytidine deaminase activity and at least a second domain that confers either a specific or non-specific DNA binding activity. The molecules of the invention are useful in somatic cell nuclear transfer and also in cancer therapy.Type: GrantFiled: October 5, 2012Date of Patent: February 25, 2014Assignee: CellCentric LimitedInventors: Wolf Reik, Hugh Morgan, Chun-Fung Chan
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Publication number: 20140053287Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: August 14, 2013Publication date: February 20, 2014Applicant: VERENIUM CORPORATIONInventors: David P. WEINER, Lishan ZHAO, Peter LUGINBUHL, Ellen BURKE, Toby H. RICHARDSON, Paula M. HICKS
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Publication number: 20140045243Abstract: The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of ?-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.Type: ApplicationFiled: March 15, 2012Publication date: February 13, 2014Applicant: The Regents of the Uniersity of CaliforniaInventors: Elizabeth A. Znameroski, James H. Doudna Cate, N. Louise Glass
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Publication number: 20140038263Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.Type: ApplicationFiled: March 15, 2013Publication date: February 6, 2014Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventor: Butamax(TM) Advanced Biofuels LLC
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Patent number: 8637277Abstract: The present invention provides is a method for producing 3-mercaptopropionic acid from 3-mercaptopropionamide or a salt thereof with the use of an amidase. The method enables the production of 3-mercaptopropionic acid on an industrial scale through an enzymatic reaction.Type: GrantFiled: April 30, 2010Date of Patent: January 28, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Tadashi Araki, Masayuki Furuya, Hidetoshi Hayashi
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Patent number: 8637272Abstract: The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.Type: GrantFiled: March 7, 2008Date of Patent: January 28, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Yu Fujio, Fumiaki Watanabe, Yuya Takikawa, Eiji Sato
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Publication number: 20140023630Abstract: Human cystathionine ?-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine ?-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine ?-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.Type: ApplicationFiled: March 14, 2013Publication date: January 23, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADOInventor: THE REGENTS OF THE UNIVERSITY OF COLORADO
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Publication number: 20140024092Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.Type: ApplicationFiled: July 26, 2013Publication date: January 23, 2014Inventors: Fredi Bruhlmann, Laurent Fourage, Denis Wahler
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Publication number: 20140005093Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: ApplicationFiled: September 13, 2013Publication date: January 2, 2014Applicant: Novozymes A/SInventors: Sanne Schoeder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Mads Eskelund Bjornvad, Thomas Thisted
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IDENTIFICATION OF ISOPRENE SYNTHASE VARIANTS WITH IMPROVED PROPERTIES FOR THE PRODUCTION OF ISOPRENE
Publication number: 20130330796Abstract: The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.Type: ApplicationFiled: March 13, 2013Publication date: December 12, 2013Applicants: The Goodyear Tire & Rubber Company, DANISCO US INC.Inventors: Zachary Q. BECK, David A. ESTELL, Jeffrey V. MILLER, James NGAI, Christopher L. RIFE, Derek H. WELLS -
Publication number: 20130330709Abstract: The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.Type: ApplicationFiled: March 13, 2013Publication date: December 12, 2013Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Mary K. BEATTY, Kevin HAYES, Zhenglin HOU, David J. MEYER, Kishore NANNAPANENI, Christopher L. RIFE, Derek H. WELLS, Gina M. ZASTROW-HAYES
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Publication number: 20130323803Abstract: The present invention relates to compositions and methods for stable transformation of green microalgae and for production of transgenic green microalgae and/or cyanobacteria that produce extremophile enzymes as co-products during the growth of the green microalgae and/or cyanobacteria for lipid biofuel production. Thus, the present invention provides nucleic acid constructs and methods of transformation useful in the production of stably transformed green microalgae and/or cyanobacteria expressing extremophile enzymes in combination with lipid production for biofuel.Type: ApplicationFiled: February 3, 2012Publication date: December 5, 2013Applicant: North Carolina State UniversityInventors: Amy Michele Grunden, Heike Inge Ada Sederoff
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Publication number: 20130323751Abstract: This invention is related to the field of the prevention and treatment of kidney disease. The treatment of kidney disease may be tailored depending upon the need for, or expectation of, renal recovery. For example, renal recovery can be determined by monitoring urine biomarkers related to the development of chronic kidney disease. For example, a normalized time course of approximately fourteen Days measuring urinary proteins can be used to establish the risk of recovery versus non-recovery in patient's having suffered an acute kidney injury. Alternatively, the invention describes signature protein expression profiles to establish the probability of renal to recovery and/or renal non-recovery.Type: ApplicationFiled: January 20, 2012Publication date: December 5, 2013Applicant: University of Pittsburgh - of the Common-Wealth System of Higher EducationInventors: Kai Singbartl, John A. Kellum, JR.
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Patent number: 8597922Abstract: Compositions and methods for producing olefins are described herein. The olefins can be used to produced biofuels.Type: GrantFiled: April 20, 2012Date of Patent: December 3, 2013Assignee: LS9, Inc.Inventors: Murtaza F. Alibhai, Mathew A. Rude, Andreas W. Schirmer
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Publication number: 20130316401Abstract: A method for improving the thermostability of a protein includes introducing, into the protein, two or more amino acid substitutions selected from the group consisting of: (i) substitution of an arginine residue for a lysine residue, (ii) substitution of a threonine residue for a serine residue, and (iii) substitution of an alanine residue for a serine residue.Type: ApplicationFiled: July 11, 2013Publication date: November 28, 2013Applicant: AJINOMOTO CO., INC.Inventors: Yosuke Nishio, Eiichiro Kimura, Yoshihiro Usuda, Kazuho Ikeo, Yoji Nakamura, Takashi Gojobori, Yutaka Kawarabayashi, Yumi Hino, Eiichi Hori, Jun Yamazaki
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Patent number: 8580555Abstract: The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.Type: GrantFiled: April 20, 2012Date of Patent: November 12, 2013Assignee: Codexis, Inc.Inventors: Erik De Vries, Louis Clark, Scott McVicar, Erika Segraves, Shiwei Song, Kheng Lin Tan
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Publication number: 20130295632Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.Type: ApplicationFiled: October 26, 2012Publication date: November 7, 2013Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Christopher L. RIFE, Derek H. WELLS
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Patent number: 8569036Abstract: TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.Type: GrantFiled: July 12, 2006Date of Patent: October 29, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Robert Dicosimo, Sharon L. Haynie, Lixuan Lisa Huang, Fateme Sima Sariaslani, Tina K. Van Dyk, Robert J. Trotman
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Patent number: 8569469Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.Type: GrantFiled: August 16, 2010Date of Patent: October 29, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
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Patent number: 8569012Abstract: The present invention provides a method for conveniently stabilizing an aqueous acrylamide solution using a stabilizer which can be separated and removed easily. In the method for stabilizing an aqueous acrylamide solution according to the present invention, a microbial cell is added to the aqueous acrylamide solution at a dried cell weight concentration of 1 to 14000 mg/L.Type: GrantFiled: March 12, 2009Date of Patent: October 29, 2013Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Masahito Oda, Katsuo Ishii
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Patent number: 8569031Abstract: The present disclosure relates to chemically modified carbonic anhydrase polypeptides and soluble compositions, homogenous liquid formulations comprising them. The chemically modified carbonic anhydrase polypeptides have improved properties relative to the same carbonic anhydrase polypeptide that is not chemically modified including the improved properties of increased activity and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides methods of preparing the chemically modified polypeptides and methods of using the chemically modified polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering.Type: GrantFiled: December 20, 2012Date of Patent: October 29, 2013Assignee: Codexis, Inc.Inventors: Scott J. Novick, Oscar Alvizo
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Patent number: 8563276Abstract: The invention relates to a method for enzyme cleavage of polysaccharides comprising a first sequence [?4)-?-D-GlcpA-(1?4)-?-L-Rhap3 sulphate-(1?]n and a second sequence [?4)-?-L-IdopA-(1?4)-?-L-Rhap3 sulphate-(1?]m, the first and second sequences respectively comprising two monosaccharide units connected by an osidic bond, wherein said method is such that: said polysaccharide sequences are provided; a microorganism capable of producing an enzyme substance of the lyase class is provided; and said enzyme substance is brought into contact with said polysaccharide sequences in such a way as to bring about cleavage of the osidic bond according to a ?-elimination reaction. The invention is characterized in that a microorganism belonging to the bacteria of the Ochrobactrum genus is chosen for producing said enzyme substance.Type: GrantFiled: June 23, 2008Date of Patent: October 22, 2013Assignee: Universite de Picardie Jules VerneInventors: Redouan El Boutachfaiti, Patrice Pheulpin, Bernard Courtois, Josiane Courtois-Sambourg
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Patent number: 8563290Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: GrantFiled: September 6, 2012Date of Patent: October 22, 2013Assignee: Novozymes A/SInventors: Sanne Schroder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Mads Eskelund Bjornvad, Thomas Thisted
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Publication number: 20130273601Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.Type: ApplicationFiled: October 11, 2011Publication date: October 17, 2013Applicant: DSM IP ASSETS B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Publication number: 20130276166Abstract: A method of enhancement of the 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity of plants or bacteria to increase terpenes production in cells, an enhanced DXS sequence likely to be obtained by this method, a method of enhancement of production of terpenes in a host cell containing the enhanced DXS enzyme, and transgenic bacterium or plants that express this polypeptide are described.Type: ApplicationFiled: October 20, 2011Publication date: October 17, 2013Applicant: Genoplante-ValorInventors: Phlippe Hugueney, Eric Duchene, Didier Merdinoglu
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Publication number: 20130266555Abstract: The present invention provides a therapeutic agent for disc herniation, which has extremely few adverse side effects, can achieve a prolonged pain-ameliorating effect when administered in only a single dose, and can exhibit a high therapeutic effect and high safety in clinical applications. The present invention relates to a therapeutic agent for disc herniation, which is characterized by containing chondroitinase ABC as an active ingredient and being administered in such a manner that the ingredient can be administered into a human disk in an amount of 1-8 units per disk.Type: ApplicationFiled: December 13, 2011Publication date: October 10, 2013Applicant: SEIKAGAKU CORPORATIONInventors: Taiichi Sirogane, Takao Murayama, Masafumi Yaguchi
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Publication number: 20130259851Abstract: The present invention relates to the use of prokaryotic sphingosine-1-phosphate lyases (S1PL) and S1PLs that lack a transmembrane domain or of a nucleic acid encoding such an S1PL in the prevention or treatment of a disease condition associated with elevated levels of sphingosine-1-phosphate (S1P), and for which S1P elevation is directly or indirectly causative. In addition, the invention relates to a new product in the form of S1PL lacking the N-terminal loop domain.Type: ApplicationFiled: November 30, 2011Publication date: October 3, 2013Applicants: UNIVERSITAT ZURICH, UNIVERSITAT BERNInventors: Uwe Zangemeister-Wittke, Andrea Huwiler, Markus G. Grutter, Florence Bourquin
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Publication number: 20130260432Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: ApplicationFiled: March 30, 2012Publication date: October 3, 2013Applicant: Danisco US Inc.Inventors: Richard R. BOTT, Marguerite A. Cervin, James T. Kellis, JR., Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Christopher Lee Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
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Publication number: 20130263329Abstract: The invention relates to nucleic acids encoding a zingiberene synthase that enables host cells and plants to make zingiberene that is useful in fragrances and for repelling or killing insects. The invention also relates to isolated zingiberene synthases and to methods for making zingiberenes.Type: ApplicationFiled: March 6, 2013Publication date: October 3, 2013Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITYInventors: CORNELIUS BARRY, ELIANA GONZALES-VIGIL
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Publication number: 20130252310Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: ApplicationFiled: September 14, 2012Publication date: September 26, 2013Applicant: AJINOMOTO, CO., INC.Inventors: Masakazu SUGIYAMA, Kunihiko Watanabe, Shigeru Kawahara
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Publication number: 20130251700Abstract: This invention provides chromatographic methods for the purification of a cystathionine ?-Synthase (CBS) protein, particularly truncated variants thereof and compositions and pharmaceutical compositions prepared therefrom.Type: ApplicationFiled: March 14, 2013Publication date: September 26, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADOInventor: THE REGENTS OF THE UNIVERSITY OF COLORADO
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Patent number: 8541220Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.Type: GrantFiled: December 22, 2008Date of Patent: September 24, 2013Assignee: Verenium CorporationInventors: David P. Weiner, Ellen G. Burke, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
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Publication number: 20130244305Abstract: The present disclosure describes the efficient use of a catalyst, an enzyme for example, to provide suitable real cyclic capacity to a solvent otherwise limited by its ability to absorb and maintain a high concentration of CO2 captured from flue gas. This invention can apply to non-promoted as well as promoted solvents and to solvents with a broad range of enthalpy of reaction.Type: ApplicationFiled: April 23, 2013Publication date: September 19, 2013Inventors: Geert F. VERSTEEG, Sylvie FRADETTE, Olivera CEPERKOVIC, Frederic VITSE, Stephen A. BEDELL, Barath BABURAO
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Publication number: 20130230889Abstract: The present invention notably relates to ulvan lyases, to nucleic acid sequences coding for these ulvan lyases, to vectors comprising these coding sequences, to a method of manufacturing these ulvan lyases, as well as to a method of degrading ulvans using these ulvan lyases and applicable applications to the degradation products of the ulvans. The ulvan lyases of the present invention, or ulvanolytic protein, are notably defined as proteins of 30 or 46 kD comprising the following four sequences in their peptide sequence: PNDPNLK, LLEVGNTGTFGSTGS, DLANPDNV and WNLPE.Type: ApplicationFiled: June 16, 2011Publication date: September 5, 2013Applicant: UNIVERSITE PIERRE ET MARIE CURIEInventors: William Helbert, Pi Nyvall-Collen, Yannick Lerat, Jean-François Sassi
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Publication number: 20130224809Abstract: Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.Type: ApplicationFiled: November 21, 2012Publication date: August 29, 2013Inventors: Joerg Bohlmann, Philipp Zerbe
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Patent number: 8518669Abstract: An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an ?-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.Type: GrantFiled: November 26, 2008Date of Patent: August 27, 2013Assignee: Amano Enzyme Inc.Inventors: Takahumi Koyama, Yuzo Kojima, Kenji Kojima, Masashi Minoda
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Patent number: 8518686Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: GrantFiled: April 22, 2010Date of Patent: August 27, 2013Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Zachary Quinn Beck, Richard R. Bott, Christopher Lee Rife, Derek H. Wells, Jeffrey V. Miller
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Publication number: 20130216515Abstract: The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.Type: ApplicationFiled: March 27, 2013Publication date: August 22, 2013Applicant: OXTHERA INTELLECTUAL PROPERTY ABInventor: OXTHERA INTELLECTUAL PROPERTY AB
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Patent number: 8512989Abstract: The present disclosure relates to ?-class carbonic anhydrase polypeptides having improved properties including increased thermostability and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides formulations and uses of the polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering. Also provided are polynucleotides encoding the carbonic anhydrase polypeptides and host cells capable of expressing them.Type: GrantFiled: December 20, 2012Date of Patent: August 20, 2013Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Michael R. Benoit, Scott J. Novick
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Publication number: 20130204034Abstract: The present invention relates to an isolated, recombinant or synthetic polynucleotide encoding a polypeptide with protoilludene synthase activity and comprising a sequence selected from the group consisting of a) SEQ ID Nos. 1 or 14 of the attached sequence listing; b) a nucleic acid sequence complementary to SEQ ID Nos. 1 or 14; c) nucleic acid sequences which hybridize under stringent conditions to the nucleic acid sequences defined in a) and b) or their complementary strands, as well as to the polypeptide encoded by the isolated polynucleotide, as well as a method for the production of melleolides employing the polynucleotide or polypeptide of the invention.Type: ApplicationFiled: January 31, 2013Publication date: August 8, 2013Applicant: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.Inventor: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.