Abstract: A system and method that predicts whether a given protein-ligand pair is active or inactive and outputs a pose score classifying the propriety of the pose. A 3D bioactivity platform comprising a 3D bioactivity module and data platform scrapes empirical lab-based data that a docking simulator uses to generate a dataset from which a 3D-CNN model is trained. The model then may receive new protein-ligand pairs and determine a classification for the bioactivity and pose propriety of that protein-ligand pair. Furthermore, gradients relating to the binding affinity in the 3D model of the molecule may be used to generate profiles from which new protein targets may be determined.

Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.

Abstract: The present invention relates to a compound of the following general formula (I); or a pharmaceutically acceptable salt and/or solvate thereof, for use as drug, particularly intended for inhibiting a programmed cell death route selected from the group consisting of ferroptosis, oxytosis and cellular necroptosis. The present invention also relates to a compound of general formula (I) for use as a drug for neuroprotection as well as for preventing and/or treating disorders associated with cellular necroptosis or ferroptosis. The present invention also relates to a pharmaceutical composition comprising a compound of general formula (I), or a pharmaceutically acceptable salt and/or solvate thereof. The present invention also encompasses the use of a compound of the general formula (I) for organs preservation.

Abstract: The present invention provides methods of determining cell sensitivity to a therapeutic agent.

Abstract: Embodiments of this invention include methods for detection of markers of bladder cancer and inflammatory conditions of the bladder. Particularly, methods include detection of expression of certain genetic markers for bladder cancer and markers for detection of inflammatory conditions of the bladder. These methods provide improved detection of the markers, and provide better detection of bladder cancer and inflammatory conditions of the bladder.

Abstract: The present invention relates to a microfluidic device for creating within a cell assembly a cell-free area, comprising at least one cell chamber, wherein the at least one cell chamber comprises: —a fluid inlet for introducing fluid into the cell chamber, —a first area, —a second area, —at least one mechanical excluding means for excluding cells from the first area of the chamber and being operable between an excluding position and a releasing position optionally via an actuation line, wherein the second area of the cell chamber is outside of the operation range of the mechanical excluding means.

Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.

Abstract: A system and method for using new biomarkers to assess individual diseases is provided. In one embodiment of the present invention, absolute quantification of annotated metabolites by mass spectrometry is used to identify certain biomarkers and derivatives thereof (i.e., signatures), which are then used to screen for, diagnose, predict, prognose, and treat various diseases, including, but not limited to, breast cancer, ovarian cancer, colorectal cancer, pancreatic cancer, and acute graft-versus-host disease.

Abstract: Provided is an external composition excellent in stability and impression from use. An external composition containing (A) at least one member selected from the group consisting of ascorbic acid and salts of ascorbic acid, (B) at least one member selected from the group consisting of 3-O-ethylascorbic acid, salts of 3-O-ethylascorbic acid, and hydroxylated lecithin, and (C) polyethylene glycol is prepared.

Abstract: Methods and products are provided for determining if a subject having a tumor is at risk of metastasis of the tumor. Specifically, the methods comprise detecting phosphorylated cofilin, and both phosphorylated and non-phosphorylated cofilin; quantifying the phosphorylated cofilin, and the total of phosphorylated and nonphosphorylated cofilin; and determining if a subject having the tumor is likely to experience metastasis of the tumor, based on the ratio of the amount of detected phosphorylated cofilin:total amount of phosphorylated and non-phosphorylated cofilin detected. Further disclosed are the types of tumor metastases that can be determined using the methods provided.

Abstract: An image analysis device may include a memory storing learning data for executing image analysis, and may obtain cell image data representing a cell image including a plurality of cell objects, sequentially identify plural pieces of partial image data from the cell image data, sequentially execute a center determination process on each of the plural pieces of partial image data, classify at least one cell corresponding to at least one cell object among a plurality of cell objects by using results of the center determination process on the plural pieces of partial image data and classification data included in learning data, and output a classification result.

Abstract: The present invention relates to recombinant virulence attenuated Gram-negative bacterial strains for use in a method of treating a malignant solid tumor in a subject.

Abstract: A biosensor test strip includes a substrate having a read section and a detection section. A surface of the detection section is formed with a protrusion seat and detection channels. The protrusion seat is provided with a notch and grooves located higher than the detection channels, and the grooves are connected to the detection channels correspondingly; in addition, detection reagents are arranged in the detection channels. A lid covers the detection channels and exposes the notch of the protrusion seat to ensure the detection results of each detection channel.

Abstract: The present invention relates to the preparation of an antibody analogue capable of being activated reversibly, and uses thereof, and provides a fusion protein comprising an inactive first fragment of an antibody analogue is fused to a stimulus-induced dimerization protein.

Abstract: A protein memory cell and a protein memory system are provided. The protein memory cell includes: first and second electrodes disposed to be spaced apart from each other on a micro channel; a gap region defined between the first and second electrodes on the micro channel; an outer region defined as an opposite side to the gap region based on the first or second electrode on the micro channel; and a photosensitive protein changing conductivity between the first and second electrodes while moving between the gap region and the outer region depending on structural conversion of a chromophore.

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

Abstract: [Problem to be Solved] The object of the present invention is to develop a method of regulating a target RNA. [Solution] There is provided a fusion protein comprising a functional domain which improves the protein expression level from mRNA and a PPR protein which can bind to a target mRNA in an RNA base-selective or RNA base sequence-specific manner.

Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.

Abstract: An object of the present invention is to provide a novel preservative solution for a heme protein and a method for stabilizing a heme protein, which are effective against denaturation or degradation of a heme protein, and the present invention specifically relates to a preservative solution for a heme protein comprising a disulfonic acid or a salt thereof, and a method for stabilizing a heme protein, which involves bringing a disulfonic acid or a salt thereof into coexistence in a sample comprising a heme protein.

Abstract: In some embodiments, a biosensor is provided. The biosensor includes a sensing element and a cover disposed on the sensing element. The sensing element includes: a first substrate embedded with a photodiode; and a filter layer disposed on the substrate. The cover includes: a second substrate; an upper optical element disposed in the second substrate; and an upper reaction well disposed on the upper optical element.

Abstract: A microfluidic device includes: a base plate allowing an electromagnetic wave to pass therethrough and having no autofluorescence; a microwell array formed on the base plate and including a wall layer in which a plurality of through-holes are formed in a thickness direction; and a lid member disposed opposite to the base plate in a state of being separated from the wall layer, wherein microwells are formed by the base plate and the through-holes formed in the wall layer, and wherein the wall layer is formed of a material containing a colored component that absorbs an electromagnetic wave of a predetermined wavelength.

Abstract: An image processing device includes a processor to detect motion of objects in an observation area on the basis of a captured image. The processor also calculates amounts of each detected motion, and calculates a propagation speed, a propagation direction, or both, of the motion for pulsations of the objects.

Abstract: The present disclosure is directed to the properties of certain glycosyltransferase variants having N-terminal truncation deletions or internal deletions. Any of the mutants disclosed in here exhibit alpha-2,6-sialyltransferase enzymatic activity in the presence of CMP-activated sialic acid as co-substrate, and in the presence of a suitable acceptor site. A fundamental finding documented in the present disclosure is that these enzymes are not only capable of catalyzing transfer of a sialidyl moiety but they are also capable of catalyzing hydrolytic cleavage of terminally bound sialic acid from a glycan.

Abstract: The present invention relates to the field of cardiology and, more specifically, to a novel algorithm that can be used, in particular, in a method for determining if a drug is likely to induce a cardiac ventricular repolarisation disturbance, based on variations in electrocardiogram data.

Abstract: Methods and systems for pruning process execution logs include learning a predictive model from a set of execution traces that characterize a process, where the predictive model determines a likelihood of a given instance reaching a specified outcome; identifying attributes in the predictive model that fall below a threshold measure of relevance to the specified outcome using a processor; and removing the identified attributes from the set of execution traces.

Abstract: Nanoparticles as described herein are configured to bind to bacterial contaminants, such as Gram positive bacteria, Gram negative bacteria, and endotoxins. The nanoparticles include a core comprising a magnetic material; and a plurality of ligands attached to the core. The ligands include, for example, bis(dipicolylamine) (“DPA”) coordinated with a metal ion, e.g., Zn2+ or Cu2+, to form, e.g., bis-Zn-DPA or bis-Cu-DPA, which can bind to the bacterial contaminants. The nanoparticles can be included in compositions for use in methods and systems to separate bacterial contaminants from liquids, such as liquids, such as blood, e.g., whole or diluted blood, buffer solutions, albumin solutions, beverages for human and/or animal consumption, e.g., drinking water, liquid medications for humans and/or animals, or other liquids.

Abstract: A method for preparation of a dried blood sample for multiplexing of analytes includes the steps of mixing an Internal Standard solution with a first diluent in a vessel, the Internal Standard solution including a plurality of different Internal Standards, adding the dried blood sample to the vessel, sonicating the vessel containing the Internal Standard solution, the first diluent and the dried blood sample, and removing the dried blood sample from the vessel. The Internal Standard solution can include a plurality of Internal Standards. The Internal Standard solution can include at least 15 Internal Standards. The dried blood sample can be generated using less than 50 ?L of blood. The dried blood sample can be generated using less than 10 ?L of blood. The first diluent can include methanol or a mixture of water and methanol.

Abstract: Methods of reducing background noise in signal-generating digital assays, such as fluorescent digital immunoassays, using a colorant are disclosed. The methods utilize a binding member that specifically binds to an analyte in a biological sample. The binding member is conjugated to a signal generating compound, e.g., an enzyme, which is dissociated from the binding member. A colorant is added with a signal generating substrate, e.g., a fluorogenic or chromogenic substrate for the enzyme, or after a signal generating substrate to reduce background noise in a fluorescent digital immunoassay. The signal generated between the signal generating compound and the signal generating substrate is detected and correlated to the presence and/or concentration of the analyte.

Abstract: The invention provides a method of preparing a nucleic acid population suitable for RNA sequencing. The method involves amplifying a double-stranded DNA and a poly T sequence by using the DNA constituted of any additional nucleic acid sequence X, poly T sequence, mRNA sequence isolated from a biological sample, poly A sequence and any additional nucleic acid sequence Y in this order as a template, a first primer containing any additional nucleic acid sequence X having amine added to the 5?-terminal (and a poly T sequence), and a second primer containing any additional nucleic acid sequence Y (and a poly T sequence), followed by fractionalizing the DNA, phosphorylating the DNA, preparing cDNA by using the DNA as a template and a third primer, adding adenine (A) to the cDNA, linking a DNA, and amplifying the DNA by using the DNA as a template, a fourth primer, and a fifth primer.

Abstract: The present invention is based on the discovery of a unique 14-gene molecular prognostic signature that is useful for predicting breast cancer metastasis. In particular, the present invention relates to methods and reagents for detecting and profiling the expression levels of these genes, and methods of using the expression level information in predicting risk of breast cancer metastasis.

Abstract: A computer-implemented method includes controlling an instrument to measure a fluorescence emission spectrum of a sample including a first peak emission wavelength and at least a second peak emission wavelength, emitted in response to an excitation wavelength and controlling the instrument to measure an absorbance obtained at the excitation wavelength of the sample. The method may include determining, using the computer, a ratio of the measurements at either the second peak emission wavelength, or a sum of measurements at a plurality of peak emission wavelengths including at least the first peak emission wavelength and the second peak emission wavelength, to the first peak emission wavelength, and calculating, using the computer, a value for a quality parameter based on a combination of at least the ratio and the absorbance measurement. The method may include controlling an associated process based on the quality parameter.

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

Abstract: A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.

Abstract: A multi-well plate allows for improvement in accuracy of experiments or tests. The present invention relates to a multi-well plate comprising: a top portion placed in a horizontal direction; a bottom portion opposing the top portion in a direction crossing the horizontal direction; and wells each recessed from the top portion toward the bottom portion, wherein the wells are horizontally arranged in a matrix with rows and columns, and each well has an opening formed at the top portion, and a peripheral wall extending from a periphery of the opening to the bottom portion. In the multi-well plate, at least two wells respectively include marks each containing at least one symbol, and each mark is placed at an opening peripheral wall end portion close to the corresponding opening.

Abstract: A deterministic lateral displacement array that includes a channel, within a substrate, having a first sidewall, a second sidewall, and a channel length. A condenser portion that includes an entry port and an exit port. A first array of pillars is disposed between the entry port and the exit port of the condenser portion along the channel length, the first array of pillars operative to drive a first material particle and a second material particle towards the first sidewall of the channel. A separator portion that includes an entry port and an exit port, and a second array of pillars disposed between the entry port and the exit port of the separator portion along the channel length, the pillars operative to drive the first material particle towards the second sidewall of the channel.

Abstract: Genetically modified mice and methods for making an using them are provided, wherein the mice comprise a replacement of all or substantially all immunoglobulin heavy chain V gene segments, D gene segments, and J gene segments with at least one light chain V gene segment and at least one light chain J gene segment. Mice that make binding proteins that comprise a light chain variable domain operably linked to a heavy chain constant region are provided. Binding proteins that contain an immunoglobulin light chain variable domain, including a somatically hypermutated light chain variable domain, fused with a heavy chain constant region, are provided. Modified cells, embryos, and mice that encode sequences for making the binding proteins are provided.

Abstract: A process for recovering one or more blastocysts from a uterus of a human includes a step of placing a device transvaginally into a cervical canal of the patient; a step of delivering fluid through the device to the uterus and applying a vacuum to a uterus of the patient to aspirate fluid and entrained one or more blastocysts from the uterus; and a step of performing a biopsy procedure on the one or more recovered blastocysts to remove one or more of trophectoderm cells or inner cell mass from the recovered blastocysts. The process may further include a step of storing one or more of the biopsied trophectoderm cells or inner cell mass following the biopsy procedure and a step of performing at least one molecular diagnostic assay test on the one or more of the biopsied trophectoderm cells or inner cell mass.

Abstract: Methods of inducing tumor regression, inhibiting tumor growth, and inducing apoptosis with selective peptidomimetic inhibitors of geranylgeranyltransferase I (GGTase I), are provided. In one aspect, GGTI-2418 and its methylester GGTI-2417, increase levels of the cyclin-dependent kinase (Cdk) inhibitor p27Kip1 and induce breast tumor regression in vivo. In another aspect, GGTI-2417 inhibits the Cdk2-mediated phosphorylation of p27Kip1 at Thr187 and accumulates p27Kip1 in the nucleus.

Abstract: The invention relates to a method for identifying marker proteins for the purpose of diagnosis and risk stratification of blood coagulation disorders.

Abstract: Systems and methods for purification and concentration of autologous alpha-2-macroglobulin (A2M) from whole blood are provided. Also provided are diagnostic methods for identifying sites in the synovial joints, spine, tendons or ligaments for treatment of pain, degeneration, or inflammation with autologous A2M. Methods for utilizing autologous A2M in combination with other autologous treatments (e.g. platelets and other growth factors) are provided in addition to combinations with exogenous drugs or carriers. Also provided is a method of producing recombinant A2M wild type or variants thereof where the bait region was modified to enhance the inhibition characteristics of A2M and/or to prolong the half life of the protein in joints and spine disc or epidural space.

Abstract: The present invention relates to a method for preparing a medical solution from at least one first liquid component and at least one second liquid component, wherein the first component and the second component are conveyed with a respective conveying means to obtain a mixed solution, wherein the conveying means are operated such that a modulation of the concentration of the first and second components takes place and the conductivity or a parameter of the mixed solution correlating with the conductivity is measured at a measurement point, wherein the modulation of the concentrations takes place in a desired state such that no modulation or a specific desired modulation of the measured conductivity or of the parameter correlated with the conductivity occurs. The present invention furthermore relates to an apparatus for preparing a medical solution as well as to a blood treatment device having such an apparatus.

Abstract: The invention proposes a device for analyzing nucleic acid molecules (M), comprising: —a bead (20), on which one molecule can be anchored at one end, —a surface (520), on which the molecule can be anchored at the other end, —an actuator (30), adapted to cause the bead to move relative to said surface in one direction, —a sensor (50), adapted to measure a distance between the bead and the surface, the device further comprising a well (11), having an axis (X-X) extending along the direction of motion of the bead and a bottom (110) formed by said surface, said well being filled with electrically conductive solution (40), and receiving the bead, the sensor being adapted to measure an impedance of the well, depending on a distance between the bead and the surface, to determine, the distance between the bead and the surface.

Abstract: A method for controlling electrical power consumption for a first group of functional which can be used for operational management during operation of the bioreactor components during operation comprises, inter alia, adjusting a present power control signal for one or more of the functional components from the first group in order to optimise power consumption, when a comparison shows for the first group of functional components that the currently required total electrical power consumption is greater than a predefined total electrical power consumption, such that, for the first group of functional components, an adjusted total electrical power consumption is not greater than the predefined total electrical power consumption. A biotechnological apparatus comprises a bioreactor, a reactor vessel formed in the bioreactor and having a cultivation chamber, and a temperature control device provided with a heat pump and configured to control the temperature of the cultivation chamber.

Abstract: A method of managing an allocation of medical examinations. The method comprises documenting, in at least one dataset, for each one of a plurality of individuals of a certain population, a plurality of historical medical test results, calculating, using a computerized processor, a plurality of relative scores each indicative of a risk of having a medical condition for one of the plurality of individuals based on respective the plurality of historical medical test results, each one of the plurality of relative scores is relative to the other of the plurality of relative scores, providing a population examination framework defining at least one criterion for selecting an individual, from the plurality of individuals, for a diagnosis of the medical condition, selecting a subgroup of the plurality of individuals according to the population examination framework, and designating members of the subgroup to perform the medical examinations.

Abstract: An apparatus for amplifying a nucleic acid sequence in a polymerase chain reaction (PCR) is provided. The apparatus comprises a microprocessor configured to communicatively couple with a user input device and receive instructions from the user input device and implement the received instructions; an electromagnetic radiation (EMR) generator configured to generated EMR at frequencies ranging from 300 THz to 400 THz and communicatively coupled with the microprocessor; a reaction vessel, communicatively coupled with the microprocessor, to house the nucleic acid sequence, necessary reagents to the PCR, and particles comprising a transition metal material, and to receive the EMR generated by the EMR generator; and a temperature sensor communicatively coupled with the reaction vessel and the microprocessor.

Abstract: An observation system includes a microscope optical system, an image pickup unit, an image pickup control unit, a detection unit, and an observation control unit. The image pickup unit is configured to take an image of a field-of-view range of the microscope optical system. The image pickup control unit is configured to cause the image pickup unit to take images of an observation sample in the field-of-view range at a plurality of focal positions and generate detection images. The detection unit is configured to detect a three-dimensional position of an observation target object in the observation sample from the detection images. The observation control unit is configured to fit the field-of-view range of the microscope optical system to the three-dimensional position.

Abstract: Methods of determining whether specific drugs or patients carry an increased risk of causing or developing, respectively, long QT syndrome or Torsades de Pointes and methods of treating such patients.

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Abstract: The present invention provides methods to determine whether a patient with a lysosomal storage disorder will benefit from treatment with a specific pharmacological chaperone. The present invention exemplifies an in vitro method for determining ?-galactosidase A responsiveness to a pharmacological chaperone such as 1-deoxygalactonojirimycin in a cell line expressing a mutant from of ?-galactosidase A. The invention also provides a method for diagnosing Fabry disease in patients suspected of having Fabry disease.