Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

Abstract: A longitudinal magnetic recording medium comprising at least four magnetic layers including a substrate, a CoCrTa magnetic intermediate layer on the substrate, wherein the CoCrTa magnetic intermediate layer comprises Cr<16 at % and Ta<6 at %, a first CoCrPtB magnetic layer on the CoCrTa layer, wherein the first CoCrPtB magnetic layer comprises Cr: 10-14%, Pt: 4-8 at %, B: 6-10%, a second CoCrPtB magnetic layer on the first CoCrPtB magnetic layer, and a third CoCrPtB magnetic layer on the second CoCrPtB layer, wherein the third CoCrPtB magnetic layer comprises Cr: 12-16 at %, Pt: 12-16 at %, B: 10-14 at % and has <10 at % Cr than that in the second CoCrPtB magnetic layer is disclosed.

Abstract: A gas liquefaction plant includes a pre-cooling exchanger which pre-cools a feed gas by means of indirect heat exchange with a first refrigerant; a first refrigerant compressor which compresses the first refrigerant which has been used for refrigerating the feed gas in the pre-cooling exchanger; a cryogenic heat exchanger which refrigerates and liquefies the feed gas which has been pre-cooled by the pre-cooling exchanger by means of indirect heat exchange with a second refrigerant; a second refrigerant compressor which refrigerates the second refrigerant which has been used for cooling and liquefying the feed gas in the cryogenic heat exchanger; and a piping complex which receives piping used in the gas liquefaction plant, wherein the pre-cooling exchanger, the first refrigerant compressor, the cryogenic heat exchanger, and the second refrigerant compressor are installed at one side of the piping complex.

Abstract: A fully differential class D amplifier is provided. The class D amplifier includes an active amplifier in the feedback path of the modulator. In one embodiment, the class D amplifier includes a fully differential amplifier as an input buffer, in which a supply-independent reference voltage is used as the common mode voltage of the output of the fully differential amplifier. In one embodiment, the class D amplifier includes a pulse width modulation circuit that includes rail-to-rail comparators.

Abstract: A system includes a lift chair having sensors embedded therein to determine various factors such as the amount of pressure exerted in various portions of the chair, the activity level of the chair user, whether the chair is occupied and which user is currently occupying the chair, and how much assistance the chair provides the user. The information detected by the sensors can be transmitted via the Internet, for example, to a third party device, such as a doctor's personal computer, which is also hooked up to the internet and is capable of receiving periodic updates to monitor use of the chair or modify the rules that govern the use of the chair. The user can override the pre-set rules for use of the chair by using an override button on a user control device.

Abstract: A humidity sensor and a quartz crystal microbalance (QCM) therefor, for measuring low humidity levels, and a method for preparing same. The metallic surface of the QCM is electroplated with a hygroscopic layer. The hygroscopic layer preferably includes Manganese. A salt is formed on the hygroscopic layer. The salt is preferably salt of Manganese, and most preferably Manganese Chloride (MnCl2).

Abstract: A switch array circuit that enables voltage regulation by bucking a relatively larger input voltage as it declines over time with different fractional gains that are based on different gain phase arrangements for a plurality of capacitors. A common rest phase is provided during the switching between the different gain phases. The rest phase inherently enables power to be conserved during gain transitions. Increasingly larger fractional gain phases (less buck) is provided as the input voltage declines over time, e.g., from ? to ? to ½ to ? to unity, and the like. Also, the common rest phase for the plurality of capacitors is arranged to minimize fluctuation of the output voltage during switching between phases to generate a selected gain from the gain phase. Additionally, the common rest phase conserves/stores energy during switching transitions between multiple gain phases. The stored energy in the common rest phase can be subsequently reused in the gain phases.

Abstract: A method of filtering optical signals (300) utilizing an optical fiber (100A-100D). The method of filtering optical signals (300) includes the steps (304) selecting an optical fiber (100A-100D) coupled to a source of optical signals, (306) disposing a core (102) in the bore (103) of the optical fiber (100A-100D) formed of a core material (105), (308) selecting a core material (105) to provide a waveguide within the optical fiber (100A-100D), (310) disposing an optical grating (114-1) in a first optical cladding layer (104) disposed about the core (102), (312) propagating an optical signal within the optical fiber (100A-100D) guided substantially within the core (102), (314) modifying a propagation path of selected wavelengths comprising said optical signal with the optical grating (114-1), and (316) determining selected wavelengths for which the propagation path is modified by selectively varying an energetic stimulus to the core (102) thereby tuning the waveguide.

Abstract: A fiber-optical, wavelength selective switch, especially for channel blocking applications. The input signal is converted to light beams having predefined polarizations relative to the plane in which optical manipulation of the beam is performed. The beams are then preferably laterally expanded in this system plane only, and then spatially dispersed in the beam expansion plane, preferably by means of a diffraction grating. The light is directed through a polarization rotation device, preferably a liquid crystal cell, pixelated along the wavelength dispersive direction such that each pixel operates on a separate wavelength. When the appropriate control voltage is applied to a pixel, the polarization of the light signal passing through that pixel is rotated. The wavelength dispersed beams from all of the pixels are then recombined, and are passed towards a polarization selective device, aligned such that only selected polarization components are transmitted out of the switch.

Abstract: An optical micro-array reader system includes microchip, VCSEL, microlens and detector arrays. The microchip array includes multiple sample spots to be separately analyzed. The VCSEL array is disposed to simultaneously illuminate more than one of the multiple spots. The microlens array focuses fluorescences or other optical emissions from the sample spots onto the detector array.

Abstract: Apparatus is provided including a frame configured to be worn on a face, such that a portion of the frame is located in front of a first eye and not in front of a second eye. The apparatus also includes a first lens, including one or more first lenses, coupled to the portion of the frame, and a second lens system, including one or more second lenses, coupled to the portion. A transparency regulation element coupled to the first lens system has variable transparency, and is configured to enhance viewing through the second lens system. A power unit is configured to drive the transparency regulation element to darken the first lens system without substantially darkening at least a portion of the second lens system during a first period, and, during a second period after the first period, to not drive the transparency regulation element to darken the first lens system.

Abstract: An improved chromatographic method for measuring impurities in a gas sample that allows extraction of a peak of impurity masked by the sample background. An impurity peak is extracted from the sample background and put in a second sample loop and the second sample loop volume is injected into a second separation column. A “slice” is taken from the sample background to fill the second sample loop and the “slice”, whose width is preferably substantially equal to the impurities peak width, is injected into the second separation column. Another embodiment allows concentration of a predetermined impurity, thereby providing an improved precision on the results. The chromatographic method provides an improved measure of argon in oxygen, oxygen in argon and oxygen in hydrogen.

Abstract: A process for killing microorganisms and controlling biofouling in high chlorine demand waters comprises mixing two components, one of which is an oxidant and the other an ammonium salt, and adding the mixture immediately to the aqueous system to be treated.

Abstract: An integrated light emitting semiconductor device having integrated feedback for wavelength locking comprises a semiconductor substrate including a waveguide region having a gain section having a gain media therein, and an out-coupling perturbation integrated with the device disposed proximate to the waveguide. A total internal reflection (TIR) structure for providing optical feedback is integrated with the device and disposed in a spaced apart relation relative to the out-coupling perturbation. At least one of the out-coupling perturbation and the TIR structure include a grating or prism to provide dispersion to send different wavelengths of light in different directions. The out-coupling perturbation is preferably a grating that has a grating period that is small enough to allow for only the +1 diffracted order to propagate.

Abstract: A method of manufacturing electronic circuits including generating CAD data, a bill of materials and an approved component vendor list for an electronic circuit and employing the CAD data, the bill of materials and the approved component vendor list for automatically generating a pick & place machine-specific component loading specification, a pick & place machine-specific component placement sequence and pick & place machine-specific component data for governing the operation of at least one specific pick & place machine in a manufacturing line.

Abstract: An interactive Web book (“ibook”) system is provided that allows material to be contributed to the World Wide Web. An ibook is a self-extending, self-sustaining information-redistributing Web robot, which is resident on a data network such as the Internet or an intranet. Users may enroll with an ibook as viewers or contributors. Viewers may view ibook material, such as text or multimedia content. Contributors may contribute original material to the ibook or may create derivations of existing ibook material. Attribution information that identifies the source of material in a derivation is automatically generated. Information concerning the derivation of each work and its characteristics can be used to help the user navigate through ibook material. The ibook system keeps track of how often users access each work within an ibook. Contributors may be automatically rewarded (e.g., by a monetary distribution) based on the extent to which their contributed material is viewed by the users.

Abstract: A method and apparatus for fabrication of passivated microfluidic structures is disclosed. The method includes providing a substrate having a microfluidic structure formed therein. The microfluidic structure is embedded by an embedding layer. The method further includes passivating the embedded microfluidic structure by locally heating the microfluidic structure surface in a reactive atmosphere, wherein the passivated microfluidic structure is suitable for transporting a fluid. The structure optionally further includes metal pads to form an electrokinetic pump.