Abstract: The present invention provides a bipolar coagulator which can be passed through the internal lumen of a ventricular catheter previously implanted into a cranial ventricle of a living subject and engaged in-situ. The bipolar coagulator will provide bipolar electrical arc currents for coagulation cauterization of adherent brain tissues, such as the choroid plexus, which occludes fluid flow into the intake drainage holes in the implanted ventricular catheter and often becomes adherent to the catheter in-situ. The cautery current provided by the bipolar coagulator is direction oriented and spatially controlled; thereby providing a better distribution of electrical current and heat within the surrounding cranial tissues; and thereby avoiding major complications of damage to intracranial structures such as blood vessels as well as avoiding the severe subarachnoid hemorrhages which are typical using other kinds of coagulation instruments.

Abstract: The present invention provides a catheter apparatus, an improved introducer system, and a methodology for creating a bypass on-demand between an unobstructed blood vessel such as the aorta and an obstructed blood vessel such as an obstructed coronary artery in-vivo using a prepared shape-memory alloy cuff and a graft segment in tandem as a shunt. The invention allows the placement and creation of single or multiple bypass grafts without use of a heart-lung machine and without need for stopping the heart of the patient during the coronary artery bypass surgery.

Abstract: The present invention provides devices, systems, assemblies, and methods intended for the introduction and sutureless juncture of a prepared communication channel to the interior spatial volume of a blood vessel or a hollow organ within a living subject. The introducer assembly and system is functional and suitable as a complete substitute and replacement for conventionally used apparatus and methods for performing vascular bypass graft surgery in order to overcome an obstruction in a major artery or vein in-vivo. The introducer assembly and system is also most appropriate for use in providing penetration and juncture of a tubular conduit for use as an access duct in order to drain materials from or introduce fluids into the interior spatial volume of a hollow organ in-vivo.

Abstract: The present invention provides genetically altered mammalian embryonic stem cells, their living descendent progeny having an altered genomic DNA, and therapeutic methods using these cells for improving cardiac function in a living subject after myocardial infarction. The genetically altered embryonic stem and progenitor cells may be maintained in-vitro as a stable cell line; and transplanted as active, mitotic cells to an infarcted area of the myocardial using any surgical procedure. After transplantation at a chosen anatomic site within the heart of the subject, these genetically altered cells will differentiate in-site, cause a regeneration of myocardocytes, and will effect a marked improvement in cardiac function for the subject.

Abstract: The present invention provides a method for inhibiting tumor angiogenesis in a living subject. The method relies upon tumor angiogenesis mediated by vascular endothelial growth factor and specified induced integrin cell surface receptors expressed on the endothelial cells of tumor-included and tumor-associated blood vessels. The methodology also administers at least one antagonistic preparation effective against specified induced and expressed integrin heterodimers on the endothelial cell surface of the living subjects, the consequence of which results in an effective inhibition of tumor angiogenesis in-vivo.

Abstract: The present invention provides a method for making an infection-resistant fabricated textile article which is suitable for any in-vivo usage either as a topical bandage, on an implantable configured construct, or as part of a prosthetic mechanical appliance. The method of manufacture applies broadly to any and all fabrics, cloths, gauzes, and/or films comprised in whole or in part of fibers; and provides an infection-resistant textile of valued use in a wide range and variety of medical applications.

Abstract: The present invention is a method for markedly improving cardiac function and repairing heart tissue in a living mammalian subject after the occurrence of a myocardial infarction. The method is a surgical technique which introduces and implants mammalian embryonic stem cells into the infarcted area of the myocardium. After implantation, the embryonic stem cells form stable grafts and survive indefinitely within the infarcted area of the heart in the living host. The demonstrated beneficial effects of the method include a decreased infarcted area and improved cardiac function as assessed by hemodynamic and echocardiographic measurements.

Abstract: A system of diagnostic test methods are provided for diagnosing whether a primary breast tumor from an individual human subject is a clinically metastatic tumor. These in-vitro diagnostic methods detect and utilize the presence or absence of a 55 kilodalton protein for the DNA or the RNA coding for an expressing this protein as a marker and indicator of tumor metastasis. The test methods thus can detect either the presence of the protein itself, or its unique DNA, or its singular RNA individually or collectively. Each method of detection provides a reliable indicator and marker by which to clinically diagnose and determine if a primary tumor within the breast of a living human subject is now or will soon likely be a metastatic tumor.

Abstract: The invention relates to novel targeting drug agents that are targeted for entry into the mitochondria. More specifically, the agents are cisplatin derivatives called mitoplatins which are useful as anti-tumor agents. Mitoplatins are named for their targeting to the mitochondrial DNA via the carnitine-acylcarnitine translocase system. The invention also relates to methods of synthesizing mitoplatins, compositions of matter containing mitoplatins and methods of using the mitoplatins.

Abstract: A system of diagnostic test methods are provided for diagnosing whether a primary breast tumor from an individual human subject is a clinically metastatic tumor. These in-vitro diagnostic methods detect and utilize the presence or absence of a 55 kilodalton protein for the DNA or the RNA coding for an expressing this protein as a marker and indicator of tumor metastasis. The test methods thus can detect either the presence of the protein itself, or its unique DNA, or its singular RNA individually or collectively. Each method of detection provides a reliable indicator and marker by which to clinically diagnose and determine if a primary tumor within the breast of a living human subject is now or will soon likely be a metastatic tumor.

Abstract: The present invention provides catheter apparatus and catheterization methodology for generating an arteriovenous fistula or a veno-venous fistula on-demand between closely associated blood vessels and at a chosen anatomic site in-vivo. The catheter apparatus is preferably employed in pairs, each catheter of the pair being suitable for percutaneous introduction into and extension through a blood vessel. The catheterization methodology employs the catheter apparatus preferably in conjunction with conventional radiological techniques in order to place, verify, and confirm a proper alignment, orientation, and positioning for the catheters in-vivo prior to activating the perforation means for generating a fistula.

Abstract: The present invention is a spatially aligned conjugated composition which comprises at least one chemically modified substance which is immunologically representative of a prechosen infectious agent and provides a chemical constituent for entering into and forming a thioether bond; a plurality of chemically substituted metallic oxide particles which range from about 10-10,000 nanometers and are able to enter into a thioether bond and covalent linkage; and at least one thioether bond and linkage joining the metallic oxide particles in a controlled and spatially aligned manner to the antigen or hapten. The conjugated composition may be alternatively employed as an immunogen; as a vaccine; as a diagnostic tool and reactant; and as an analytical material suitable for testing the pharmacological activity of new compounds.

Abstract: The present invention provides a unique DNA segment and a previously unknown PDZK1 protein as a marker system indicative of cells and tissues in an abnormal cellular state and which are undergoing a progressive loss of cell regulation and control. The isolated DNA sequence encodes the PDZK1 protein; and the expressed PDZK1 protein, particularly when present in large or excessive quantitative amounts, is a reliable and reproducible biochemical marker that an ongoing neoplastic development is occurring within cell and tissues of epithelial cell origin.

Abstract: The present invention provides an immunological preparation comprising not less than two types of conjugate molecules in admixture for concurrent specific binding to a spatially exposed region of vascular permeability factor (VPF) bound in-vivo to a tumor-associated blood vessel. Each conjugate molecule type comprises at least a binding portion of an antibody specific for an epitope present within a spatially exposed region of bound VPF; and an effector moiety covalently bound to the specific binding portion. The immunological preparation has wide uses and applications including analytical studies, in-vivo diagnostic testing, and in-vivo therapeutic treatments.

Abstract: The present invention provides a catheter apparatus, an improved introducer system, and a methodology for creating a bypass on-demand between an unobstructed blood vessel such as the aorta and an obstructed blood vessel such as an obstructed coronary artery in-vivo using a prepared shape-memory alloy cuff and a graft segment in tandem as a shunt. The invention allows the placement and creation of single or multiple bypass grafts without use of a heart-lung machine and without need for stopping the heart of the patient during the coronary artery bypass surgery.

Abstract: The present invention provides stabilizing formulations for maintaining and preserving the integrity of proteins and polypeptides present in a body fluid sample obtained ex-vivo and to be evaluated as a test specimen for either clinical, therapeutic, or research purposes. The stabilizing formulations may be prepared alternatively either as a dry, anhydrous mixture of powders or as an aqueous based liquid containing the dissolved ingredients in admixture. The invention also provides minimalist stabilizing formulations as well as fortified stabilizing formulations which meet specific uses and applications and may be advantageously employed over a wide variety of different time, temperature, and severity of conditions.

Abstract: Stable clones of neural stem cells (NSCs) have been isolated from the human fetal telencephalon. In vitro, these self-renewing clones (affirmed by retroviral insertion site) can spontaneously give rise to all 3 fundamental neural cell types (neurons, oligodendrocytes, astrocytes). Following transplantation into germinal zones of the developing newborn mouse brain, they, like their rodent counterparts, can participate in aspects of normal development, including migration along well-established migratory pathways to disseminated CNS regions, differentiation into multiple developmentally- and regionally-appropriate cell types in response to microenvironmental cues, and non-disruptive, non-tumorigenic interspersion with host progenitors and their progeny. Readily genetically engineered prior to transplantation, human NSCs are capable of expressing foreign transgenes in vivo in these disseminated locations.

Abstract: New compounds that bind specifically to vascular permeability factor (VPF) are used in methods of targeting these compounds, which include effector molecules that are, e.g., toxic, radioactive, or serve as marker labels, for tumor cells and the associated blood vessel endothelial cells, based on the discovery that VPF concentrates selectively in the endothelium and basement membrane lining tumor-associated blood vessels to a far greater degree than in normal vessels. By targeting VPF rather than the tumor cells themselves, the invention avoids the problems of tumor heterogeneity and diffusion distance.

Abstract: The present invention provides catheter apparatus and catheterization methodology for generating an arteriovenous fistula or a veno-venous fistula on-demand between closely associated blood vessels and at a chosen anatomic site in-vivo. The catheter apparatus is preferably employed in pairs, each catheter of the pair being suitable for percutaneous introduction into and extension through a blood vessel. The catheterization methodology employs the catheter apparatus preferably in conjunction with conventional radiological techniques in order to place, verify, and confirm a proper alignment, orientation, and positioning for the catheters in-vivo prior to activating the perforation means for generating a fistula.

Abstract: The present invention provides unique apparatus, systems and methods for an aligned closing and stabilized sealing of a vascular perforation in a chosen blood vessel after percutaneous puncture and completion of a catheterization procedure. The invention is an adaptation of the conventionally known modified Seldinger technique in which the means for guidance, closing and stabilized sealing are deployed and positioned during the manipulations performed during the percutaneous puncture of the blood vessel itself.