Patents Represented by Attorney, Agent or Law Firm Elias J Lambiris
  • Enzymes with aminopeptidase activity
    Patent number: 6413559
    Abstract: The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.
    Type: Grant
    Filed: November 22, 2000
    Date of Patent: July 2, 2002
    Assignee: Novozymes A/S
    Inventors: Markus Sakari Kauppinen, Joan Qi Si, Tina Spendler, Claus Dambmann, Torben Halkier, Peter Rahbek Østergaard, Shamkant Anant Patkar, Kim Hansen
  • Process for removal of excess dye from printed or dyed fabric or yarn
    Patent number: 6409771
    Abstract: The present invention provides a process for removal of excess dye from newly manufactured printed or dyed fabric or yarn, comprising treatment with a rinse liquor comprising at least one enzyme selected from the group consisting of enzymes exhibiting peroxidase activity or laccase activity, an oxidation agent, and at least one mediator comprising N-hydroxyacetanilide.
    Type: Grant
    Filed: May 11, 2001
    Date of Patent: June 25, 2002
    Assignee: Novozymes A/S
    Inventors: Ture Damhus, Uwe Vogt, Palle Schneider
  • Endoglucanases
    Patent number: 6387690
    Abstract: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequence Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequence Trp Cys Cys Xaa Cys 1   2   3   4   5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in
    Type: Grant
    Filed: January 13, 1999
    Date of Patent: May 14, 2002
    Assignee: Novozymes A/S
    Inventors: Martin Schülein, Lene Nonboe Andersen, Søren Flensted Lassen, Markus Sakari Kauppinen, Lene Lange, Ruby Ilum Nielsen, Michiko Ihara, Shinobu Takagi
  • Acidic cleaning composition comprising an acidic protease I
    Patent number: 6376449
    Abstract: Acidic detergent composition comprising (a) protease I obtainable from Aspergillus aculeatus and having a pH optimum of 3 to 6 and (b) at least one nonionic surfactant and methods for using the composition for cleaning or washing a hard surface or laundry.
    Type: Grant
    Filed: March 23, 1999
    Date of Patent: April 23, 2002
    Assignee: Novozymes A/S
    Inventor: Hans Sejr Olsen
  • Haloperoxidases with altered pH profiles
    Patent number: 6372465
    Abstract: The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.
    Type: Grant
    Filed: February 20, 2001
    Date of Patent: April 16, 2002
    Assignee: Novozymes A/S
    Inventors: Allan Svendsen, Louise Jørgensen
  • Methods of producing polynucleotide variants
    Patent number: 6368805
    Abstract: A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.
    Type: Grant
    Filed: August 23, 2000
    Date of Patent: April 9, 2002
    Assignee: Novozymes A/S
    Inventors: Torben Vedel Borchert, Titus Kretzschmar, Joel R. Cherry, Vind Jesper
  • Preparation of dough and baked products
    Patent number: 6365204
    Abstract: Disclosed is a process for preparing a dough or a baked product from the dough which process involves incorporating into the dough an anti-staling amylase and a phospholipase. The bread made by the combined use of an anti-staling amylase and a phospholipase has improved softness, both when eaten on the same day and when stored for several days after baking. There is no significant change in the taste or smell of the baked product.
    Type: Grant
    Filed: April 5, 1999
    Date of Patent: April 2, 2002
    Assignee: Novozymes
    Inventors: Tina Spendler, Lone Nilsson, Claus Crone Fuglsang
  • Oral care product comprising a mutan binding domain
    Patent number: 6355228
    Abstract: Disclosed is a polypeptide hybrid containing an amino acid sequence with binding affinity for mutan, the amino acid sequence being bound to an active component useful for oral care purposes; an oral care composition comprising a mutan binding domain; an oral care product comprising such an oral care composition of the invention; and finally the use of a mutan binding polypeptide hybrid or a single unit MBD for oral care purposes, including preventing dental plaque formation and/or removal of existing dental plaque.
    Type: Grant
    Filed: April 20, 1999
    Date of Patent: March 12, 2002
    Assignee: Novozymes A/S
    Inventor: Claus Crone Fuglsang
  • Host cells and methods of producing proteins
    Patent number: 6352841
    Abstract: The present invention relates to novel host cells and to methods of producing proteins. More specifically the invention relates to a host cell useful for the expression of heterologous proteins, in which the host cell has been genetically modified in order to express significantly reduced levels of a metalloprotease and an alkaline protease. Moreover the invention relates to a method of producing a heterologous protein, which method comprises cultivating the host cell in a suitable growth medium, followed by recovery of the desired protein.
    Type: Grant
    Filed: February 18, 1999
    Date of Patent: March 5, 2002
    Assignee: Novozymes A/S
    Inventor: Jan Lehmbeck
  • Alkaline lipolytic enzyme
    Patent number: 6350604
    Abstract: Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.
    Type: Grant
    Filed: October 22, 1998
    Date of Patent: February 26, 2002
    Assignee: Novozymes A/S
    Inventors: Satoshi Hirayama, Rikako Taira, Kim Borch, Thomas Sandal, Torben Halkier, Karen Margrethe Oxenbøll, Bjarne Rønfeldt Nielsen
  • Enzyme containing granule
    Patent number: 6348442
    Abstract: This invention relates to an enzyme containing granular composition comprising: a) an enzyme containing core and b) a protective substantially continuous layer or coating encapsulating the core comprising at least 60% of a water soluble compound, having a molecular weight below 500 grams per mole, a pH below 11 and a constant humidity at 20° C. of more than 81%. The invention provides an improved stability of enzymes upon storage.
    Type: Grant
    Filed: June 1, 2001
    Date of Patent: February 19, 2002
    Assignee: Novozymes A/S
    Inventor: Erik Kjær Markussen
  • Modification of polysaccharides by means of a phenol oxidizing enzyme
    Patent number: 6346401
    Abstract: The present invention deals with a process for oxidation of a hydroxy group of C1 and/or C2 and/or C3 and/or C4 and/or C5 and/or C6 of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.
    Type: Grant
    Filed: May 26, 1999
    Date of Patent: February 12, 2002
    Assignee: Novozymes A/S
    Inventor: Jesper Vallentin Kierulff
  • Fungal protein disulfide isomerase
    Patent number: 6346244
    Abstract: The present invention relates to protein disulfide isomerases which are encoded by a nucleic acid sequence which hybridizes with (i) the DNA sequence of SEQ ID NO:1 or (ii) the DNA sequence of SEQ ID NO:2, under the following conditions: presoaking in 5×SSC and prehybridizing for 1 h at ˜40° C. in a solution of 5×SSC, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 &mgr;g of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 &mgr;Ci 32-P-dCTP labelled probe for 18 h at ˜40° C. followed by washing three times in 2×SSC, 0.2% SDS at 40° C. for 30 minutes; and fragments thereof. The present invention also relates to DNA sequences encoding the protein disulfide isomerases, compositions comprising said protein disulfide isomerases and methods of use thereof.
    Type: Grant
    Filed: March 4, 1999
    Date of Patent: February 12, 2002
    Assignee: Novozymes A/S
    Inventor: Carsten Mailand Hjort
  • Bacterial galactanases and use thereof
    Patent number: 6331426
    Abstract: The invention relates to a method for modifying animal feed, in particular animal feed containing plant material such as soybean, by adding to the animal feed at least one galactanase enzym, to a method for obtaining a DNA sequence encoding a galactanase enzyme or a portion thereof, and to isolated polynucleotide molecules encoding polypeptides having galactanase activity.
    Type: Grant
    Filed: February 11, 2000
    Date of Patent: December 18, 2001
    Assignee: Novozymes A/S
    Inventors: Mads Eskelund Bjørnvad, Ib Groth Clausen, Martin Schülein, Lisbeth Bech, Peter Rahbek Østergaard, Carsten Sjøholm
  • Enzyme with galactanase activity
    Patent number: 6329185
    Abstract: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme composition comprising the enzyme, and the use of the enzyme and enzyme composition for a number of industrial applications.
    Type: Grant
    Filed: August 4, 1998
    Date of Patent: December 11, 2001
    Assignee: Novozymes A/S
    Inventors: Lene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen
  • In vivo recombination
    Patent number: 6326206
    Abstract: The present invention relates to a method for in vivo recombination of homologous DNA sequences. The method is a forced artificial evolution resulting in a DNA sequence encoding a polypeptide having an advantageous property.
    Type: Grant
    Filed: June 10, 1999
    Date of Patent: December 4, 2001
    Assignee: Novozymes A/S
    Inventors: Mads Eskelund Bjornvad, Michael Dolberg Rasmussen, Per Lina Jorgensen, Torben Vedel Borchert, Stanislas Dusko Ehrlich
  • Method for reducing amino acid biosynthesis inhibiting effects of a sulfonyl-urea based compound
    Patent number: 6326186
    Abstract: A method has been found for reducing amino acid biosynthesis inhibiting effect of a sulfonyl-urea based compound of the general formula: comprising contacting in an aqueous solution said sulfonyl-urea based compound with an enzyme capable of reducing said amino acid biosynthesis inhibiting effect.
    Type: Grant
    Filed: October 13, 1999
    Date of Patent: December 4, 2001
    Assignee: Novozymes A/S
    Inventors: Ole Kirk, Anders Ohmann
  • 2,6-&bgr;-D-fructan hydrolase enzyme and processes for using the enzyme
    Patent number: 6323007
    Abstract: The present invention relates to isolated polypeptides having polypeptide having 2,6-&bgr;-D-fructan hydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: September 17, 1999
    Date of Patent: November 27, 2001
    Assignee: Novozymes A/S
    Inventors: Soren Moller, Charlotte Johansen, Thomas Schafer, Peter Rahbek Ostergaard, Lisbeth Hedegaard Hoeck
  • DNA encoding a transcription factor for an &agr;-amylase promoter in filamentous fungi
    Patent number: 6316220
    Abstract: A transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.
    Type: Grant
    Filed: November 23, 1998
    Date of Patent: November 13, 2001
    Assignee: Novozymes A/S
    Inventor: Tove Christensen
  • Recovery of a glycosidase or peptidase from a culture broth at high pH
    Patent number: 6316240
    Abstract: The present invention relates to a method for recovering a glycosidase or a peptidase from a culture solution from an unsolubilized state within said solution to a solubilized state within said solution.
    Type: Grant
    Filed: January 24, 2000
    Date of Patent: November 13, 2001
    Assignee: Novozymes A/S
    Inventors: Mads Aage Laustsen, Curran Matthew Simpson, Michael John O'Reilly