Abstract: The present invention relates to a process for enzymatic hydrolysis of cyclic oligomers of poly(ethylene terephthalate), which process comprises subjecting the cyclic oligomer to the action of one or more lipolytic and/or biopolyester hydrolytic enzyme(s).
Type:
Grant
Filed:
October 2, 2000
Date of Patent:
April 15, 2003
Assignee:
Novozymes A/S
Inventors:
Martin Riegels, Rainhard Koch, Lars Saaby Pedersen, Henrik Lund
Abstract: The present invention provides methods for single-bath biopreparation and dyeing of cellulosic fibers, which are carried out by contacting the fibers simultaneously or sequentially with a bioscouring enzyme, preferably pectinase, protease, and/or lipase, and a dyeing system, under conditions that do not require emptying the bath or rinsing the fabric between biopreparation and dyeing steps.
Type:
Grant
Filed:
May 24, 2000
Date of Patent:
April 8, 2003
Assignee:
Novozymes, A/S
Inventors:
Jiyin Liu, Brian Condon, Harry Lee Showmaker, III
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
Abstract: A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby
A. extended primers are synthesized by
a) denaturing the DNA templates
b) annealing primers to the templates,
c) extending the said primers by use of a polymerase,
d) stop the synthesis, and
e) separate the extended primers from the templates,
B. a template shift is induced by
a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or
b) repeating steps A.b) to A.e),
C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof.
Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.
Abstract: The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
Type:
Grant
Filed:
April 7, 2000
Date of Patent:
March 4, 2003
Assignee:
Novozymes, A/S
Inventors:
Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.
Abstract: Phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
Type:
Grant
Filed:
March 22, 1999
Date of Patent:
February 4, 2003
Assignee:
Novozymes A/S
Inventors:
Allan Svendsen, Søren Flensted Lassen, Dirk Kostrewa, Luis Pasamontes, Martin Lehmann, Andrea Tomschy, Adolphus Van Loon, Kurt Vogel, Markus Wyss
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having proteolytic activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
Type:
Grant
Filed:
September 28, 2001
Date of Patent:
January 28, 2003
Assignee:
Novozymes, A/S
Inventors:
Helle Outtrup, Søren Flensted Lassen, Poul Erik Pedersen
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.
Abstract: The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides.
Abstract: The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
January 14, 2003
Assignee:
Novozymes, A/S
Inventors:
Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjørn Petersen, Leif Nørskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
Abstract: The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides.
Abstract: An isolated or purified polypeptide having xyloglucanase activity which is obtained from a strain of the genus Malbranchea and has xyloglucanase activity in the pH range 4-11, measured at 50° C.; and/or a molecular mass of 25±10 kDa, as determined by SDS-PAGE; and/or an isoelectric point (pI) in the range 3-5; and/or an N-terminal sequence Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu is useful in industrial applications such as in laundry detergent compositions and for treatment of textiles.
Type:
Grant
Filed:
September 1, 2000
Date of Patent:
December 31, 2002
Assignee:
Novozymes, A/S
Inventors:
Wenping Wu, Martin Schulein, Markus Sakari Kauppinen, Mary Ann Stringer
Abstract: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
Type:
Grant
Filed:
January 14, 1998
Date of Patent:
December 17, 2002
Assignee:
Novozyme A/S
Inventors:
Claus Crone Fuglsang, Jens Sigurd Okkels, Dorte Aaby Petersen, Shamkant Anant Patkar, Marianne Thellersen, Allan Svendsen, Kim Borch, John C. Royer, Titus Kretzschmar, Torben Halkier, Jesper Vind, Steen Troels Jorgensen
Abstract: The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.
Type:
Grant
Filed:
October 13, 1999
Date of Patent:
October 8, 2002
Assignee:
Novozymes, A/S
Inventors:
Arne Agerlin Olsen, Claus von der Osten, Kim Vilbour Andersen, Steffen Ernst, Erwin Ludo Roggen
Abstract: The present invention relates to a method for the separation of flour into one gluten fraction and at least one other fraction, comprising the steps of: mixing the flour and a liquid to obtain a dough, separating the dough into a fraction comprising gluten and at least one other fraction, recovering at least the gluten fraction, wherein an oxidoreductase is added at any of steps a), b) or c).
Abstract: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
Type:
Grant
Filed:
June 24, 1997
Date of Patent:
August 6, 2002
Assignee:
Novozymes A/S
Inventors:
Lisbeth Bech, Grethe Rasmussen, Torben Halkier, Mariko Okada, Lene Nonboe Andersen, Markus Sakari Kauppinen, Thomas Sandal
Abstract: The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.
Type:
Grant
Filed:
November 10, 1998
Date of Patent:
July 23, 2002
Assignee:
Novo Nordisk A/S
Inventors:
Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
Abstract: Modified enzymes are prepared for use in skin care products by covalently coupling to the enzymes from 4 to 70 polymeric molecules with or without a linker such as a triazine ring. Molecular weight of the polymeric molecules may be from 1 to 35 kDa and of the enzymes from 15 to 100 kDa. The number and weight of polymeric molecules coupled is balanced with the weight and/or surface area of the enzymes. Enzymes include proteases such as subtilisins, lipases and oxidoreductases such as laccases and superoxide dismutase, and polymeric molecules include polysaccharides such as dextran or pullulan and polyalkylene oxides such as polyethylene glycol. The polymeric molecules may be coupled to the enzymes at the N-terminal amino group and/or lysine residues, and preferably at a position more than 5 Å from the active site of the enzymes. A preferred modified enzyme is a protease having from 5 to 13 coupled polymeric molecules.