Abstract: In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virus (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.
Type:
Grant
Filed:
October 28, 1993
Date of Patent:
October 4, 1994
Assignee:
Monsanto Company
Inventors:
Robert T. Fraley, Robert B. Horsch, Stephen G. Rogers
Abstract: A class of heterologous dominant conditional lethal genes is disclosed. The gene is useful in genetically modifying plant cells and plants to selectively induce cellular lethality for purposes such as inducing male sterility for hybrid seed production, cell ablation and counter-negative selection.
Abstract: A method for potentiating the insecticidal activity of a protein toxin of Bacillus thuringiensis bacteria is disclosed. A potentiating amount of trypsin inhibitor is co-administered to the insect along with the toxin. Improved insecticidal compositions are also disclosed which contain an insecticidal amount of a protein toxin of Bacillus thuringiensis and a potentiating amount of a trypsin inhibitor.
Type:
Grant
Filed:
December 20, 1991
Date of Patent:
October 5, 1993
Assignee:
Monsanto Company
Inventors:
Roy L. Fuchs, Ganesh M. Kishore, Susan C. MacIntosh
Abstract: A transacting DNA binding factor is disclosed. The ASF 1 protein factor specifically binds to the sequence motif TGACG found upstream of the promoter in many plant genes. Coexpression of this protein factor augments the level of expression of the up-regulated promoter containing the TGACG motif.
Type:
Grant
Filed:
February 1, 1991
Date of Patent:
June 29, 1993
Assignee:
The Rockefeller University
Inventors:
Fumiaki Katagiri, Eric Lam, Nam-Hai Chua
Abstract: Novel N-[2-(cyclic alkyl)phenyl]pyrazole-4-carboxamides useful as fungicides, methods of using said compounds, and fungicidal compositions containing them.
Abstract: Disclosed herein are herbicidal pyridine compounds substituted at the 3- and/or 5-position with a carboxylic acid-derived heterocyclic moiety, as well as herbicidal compositions and use of these compounds.
Abstract: An improved process for dehydrating dialkyl 2,6-bis(trifluoromethyl)-2,6-dihydroxy-4-(alkyl)-3,5-piperidinedicarboxyla tes using concentrated sulfuric acid essentially in the absence of a solvent to prepare the corresponding dialkyl 2,6-bis(trifluoromethyl)dihydro-4-(alkyl)-3,5-pyridinedicarboxylates.
Abstract: A compound having the structural formula ##STR1## wherein: Y is selected from O and S;R.sub.1 and R.sub.4 are independently selected from fluorinated methyl, chlorofluorinated methyl, and C.sub.1 -C.sub.4 alkyl, provided that not both R.sub.1 and R.sub.4 may be C.sub.1 -C.sub.4 alkyl;R.sub.2 is selected from hydrogen, lower alkyl, haloalkyl, alkenyl, alkynyl, haloalkenyl, and a cation;R.sub.3 is selected from C.sub.1 -C.sub.5 alkyl, cycloalkyl, cycloalkylalkyl, hydroxy, and alkoxy; andX is selected from ##STR2## in which R.sub.8 is selected from H, alkyl and CF.sub.3 and R.sub.10 is selected from F, Cl, --OR.sup.1, --SR.sup.1, --NHR.sup.1, --N(R.sup.1).sub.2, phenyl, substituted phenyl, and --P(OR.sup.1).sub.2where R.sup.1 is as defined above; ##STR3## wherein R.sup.1 is as defined above; ##STR4## wherein R.sub.14 and R.sub.15 are independently selected from hydrogen and alkyl; and --N.dbd.C.dbd.O and their use as herbicides.
Abstract: This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
Type:
Grant
Filed:
February 12, 1990
Date of Patent:
February 23, 1993
Assignee:
Monsanto Company
Inventors:
Dilip M. Shah, Stephen G. Rogers, Robert B. Horsch, Robert T. Fraley
Abstract: Transgenic plants are disclosed which are resistant to virus infection by Potato Virus X and Potato Virus Y. Plant genes and transformation vectors are also disclosed. Potato plants, for example, Russet Burbank variety, are made resistant to dual infection by Potato Virus X and Potato Virus Y by transforming the plant to express the coat proteins of the two viruses.
Abstract: A method of inducing male sterility in a dicotyledonous plant which comprises treating the plant prior to, during or post meiosis with an amount of a substituted 4-oxo-1-phenyl-1,4-dihydropyridazine, which is effective to produce male sterility in the plant.
Abstract: Substituted pyridines having at the 3 or 5 position a carbonyl oxy(thio)ester or a heterocyclocarbonyl and at the other a substituted alkyl group, herbicidal compositions and methods of use thereof.
Type:
Grant
Filed:
May 23, 1991
Date of Patent:
December 8, 1992
Assignee:
Monsanto Company
Inventors:
Susan M. Auinbauh, Len F. Lee, Karey A. Van Sant
Abstract: Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.
Abstract: Processes for production of 2,6-substituted-3,5-pyridinedicarboxylic acids, esters, salts, amides, halides, and cyano compounds, useful as herbicides and as intermediates which provide herbicides.
Abstract: Disclosed herein are 2- or 6-fluoromethyl-3-pyridinecarboxylate derivatives with 5-[(heterocyclic)ylidene]amino substitution useful as herbicides and herbicide intermediates.
Type:
Grant
Filed:
May 20, 1991
Date of Patent:
July 14, 1992
Assignee:
Monsanto Company
Inventors:
Shridhar G. Hegde, Len F. Lee, Robert D. Bryant
Abstract: Substituted pyridines characterized by having been substituted in the 3-position with an isoxazolyl or isothiazolyl radical. A method of using such pyridines as herbicides and herbicidal compositions containing such pyridines are described. Methods of preparing such substituted pyridines are also disclosed.
Type:
Grant
Filed:
August 12, 1991
Date of Patent:
June 30, 1992
Assignee:
Monsanto Company
Inventors:
Susan M. Auinbauh, Len F. Lee, Lisa L. McDermott
Abstract: Disclosed herein are herbicidal pyridine compounds substituted at the 2- or 6-position with an OR.sub.2 moiety where R.sub.2 is hydrogen, lower alkyl, halomethyl, lower cyanoalkyl, alkenyl or alkynyl, as well as herbicidal compositions and herbicidal use of these compounds.