Abstract: We describe a strategy for designing and implementing protein-fragment complementation assays (PCAs) to detect biomolecular interactions in vivo and in vitro. The design, implementation and broad applications of this strategy are illustrated with a large number of enzymes with particular detail provided for the example of murine dihydrofolate reductase (DHFR). Fusion peptides consisting of N- and C-terminal fragments of murine DHFR fused to-GCN4 leucine zipper sequences were coexpressed in Escherichia coli grown in minimal medium, where the endogenous DHFR activity was inhibited with trimethoprim. Coexpression of the complementary fusion products restored colony formation. Survival only occurred when both DHFR fragments were present and contained leucine-zipper forming sequences, demonstrating that reconstitution of enzyme activity requires assistance of leucine zipper formation. DHFR fragment-interface point mutants of increasing severity (lie to Val, Ala and Gly) resulted in a sequential increase in E.

Abstract: Liquid hydrocarbons are gelled by the introduction of a phosphate ester and a crosslinking agent. Gelling times and viscosities are improved through the use of an enhancer which is, preferably, an oxyalkylated amine or a blend of an oxyalkylated amine with another amine or a non-nitrogen-containing component. Preferred phosphate esters are ether phosphate esters.

Abstract: The invention relates to compositions and methods for gelling liquid hydrocarbons by the introduction of certain phosphate esters, a crosslinking agent, and an enhancer. The enhancer of the present invention is preferably an oxyalkylated amine or a blend of an oxyalkylated amine with another amine or a non-nitrogen-containing component. Mixed phosphate esters containing an oxyalkylene group are useful in the practice of the invention. Gel-forming compositions for addition to liquid hydrocarbons, as well as methods for forming fracturing fluids, are encompassed. The present compositions and methods are an improvement in the field of hydrocarbon gelling, e.g., in fracturing formations, by providing more rapid gellation and greater viscosities.

Abstract: Nutriceutical compositions useful as a dietary supplement which have antioxidant/free radical scavengers and also having a cytoprotective effect are disclosed. The compositions contain a cytoprotective and antioxidant/free radical scavenging amount of at least one of D-histidine, L-histidine, a racemic mixture thereof, a non-racemic mixture thereof, and nutriceutically acceptable salts thereof in combination with phytonutrients having antioxidant properties such as canthanaxin, vitamin A and limonene. The compositions can be prepared in capsule form, tablets, sustained release tablets, suspensions and oral rehydration solutions.

Abstract: The invention relates to compositions and methods for gelling liquid hydrocarbons by the introduction of certain phosphate esters, a crosslinking agent, and an enhancer. The enhancer of the present invention is preferably an oxyalkylated amine or a blend of an oxyalkylated amine with another amine or a non-nitrogen-containing component. Mixed phosphate esters containing an oxyalkylene group are useful in the practice of the invention. Gel-forming compositions for addition to liquid hydrocarbons, as well as methods for forming fracturing fluids, are encompassed. The present compositions and methods are an improvement in the field of hydrocarbon gelling, e.g., in fracturing formations, by providing more rapid gellation and greater viscosities.

Abstract: The invention provides a general protein-fragment complementation assays to detect biomolecular interactions in vivo and in vitro. The protein-complemetation assay/universal reporter system can be used to detect and screen an agonist and an antagonist of a membrane receptor system. The assay can be used to study protein-protein, protein-DNA, protein-RNA, protein-carbohydrate, and protein-small molecule interactions. The assay can be used to screen cDNA libraries for binding of a target protein with unknown proteins or libraries of small organic molecules for biological activity.

Abstract: A method for planting and stabilizing soilless sods is taught, beginning with planting pure stock of the desired cultivar in sterile media over plastic sheeting. The pure stock, once it has matured into a sod, it is harvested and milled into vegetative planting material. The milled material is suspended in a liquid slurry in an agitating tank and discharged into a trailing planter assembly that spreads the slurry evenly over unrolling plastic sheeting where it acts not only as planting material but, in sufficient quantities, also as a stabilizing protective mulch that maintains the integrity of the young sod crop in severe storms. Unlike sprigs or stolons the milled planting stock is produced from whole plant tissue and is comprised of many plantlets with intact root, stem, and leaf tissue. A bushel of milled turf plantlets is much more dense than a bushel of sprigs or stolons and comprises meristems from all parts of the plant including roots, crowns, and rhizomes.

Abstract: The invention relates to compositions and methods for gelling liquid hydrocarbons by the introduction of certain phosphate esters, a crosslinking agent, and an enhancer. The enhancer of the present invention is preferably an oxyalkylated amine or a blend of an oxyalkylated amine with another amine or a non-nitrogen-containing component. Mixed phosphate esters containing an oxyalkylene group are useful in the practice of the invention. Gel-forming compositions for addition to liquid hydrocarbons, as well as methods for forming fracturing fluids, are encompassed. The present compositions and methods are an improvement in the field of hydrocarbon gelling, e.g., in fracturing formations, by providing more rapid gellation and greater viscosities.

Abstract: We describe a strategy for designing and implementing protein-fragment complementation assays (PCAs) to detect biomolecular interactions in vivo and in vitro. The design, implementation and broad applications of this strategy are illustrated with a large number of enzymes with particular detail provided for the example of murine dihydrofolate reductase (DHFR). Fusion peptides consisting of N- and C-terminal fragments of murine DHFR fused to GCN4 leucine zipper sequences were coexpressed in Escherichia coli grown in minimal medium, where the endogenous DHFR activity was inhibited with trimethoprim. Coexpression of the complementary fusion products restored colony formation. Survival only occurred when both DHFR fragments were present and contained leucine-zipper forming sequences, demonstrating that reconstitution of enzyme activity requires assistance of leucine zipper formation. DHFR fragment-interface point mutants of increasing severity (lle to Val, Ala and Gly) resulted in a sequential increase in E.

Abstract: An improved process for the treatment of water, selected from livestock wastewater and a livestock water stream, which process comprises adding to said water a composition comprising a sufficient amount of an acid to lower the water pH to less than 3.5 to 2.0; and wherein said acid is selected from the group of 1,3,5-Triazine-2,4,6-(1H,3H,5H)-hydroxyalkyltriglyoxilic acid (PR-1409SA); 2,2-oxy-diacetic acid; 2,2-ether diglycolic acid; sulfamic acid; and sulfuric acid.

Abstract: The present invention relates to methods for alleviating disorders or chronic conditions of the female reproductive system, such as dysmenorrhea, endometrial pain, and pre-term labor, through dietary supplementation with histidine. The invention relates further to novel combination supplements of histidine in conjunction with other nutritional supplement materials which are preferably also useful in alleviating the above-mentioned disorders or conditions. A method for administering a dietary histidine supplement in conjunction with one or more separately formulated therapeutic drugs also known to be useful in treating these female reproductive conditions is also disclosed.

Abstract: The present invention relates to methods and compositions for preventing or treating conditions or disorders of the female reproductive system by administering an effective dosage of histidine alone or in combination with other therapeutic agents. The invention relates also to novel physical compositions and delivery devices for administering histidine effectively to a female subject in need of either prophylaxis or treatment of certain disorders of the reproductive system.

Abstract: Water-soluble or water-dispersible and curable polyester resins are suitable as binders in various applications, The resins are reaction products of polyterephthalate, polyols, polyacids, end acids and crosslinking agents. The waste terephthalates can be utilized when making the binders of the present invention. The preferred polyol is pentaerythritol. The resins can be used as binder for glass fiber insulation products.

Abstract: Liquid hydrocarbons are gelled by the introduction of a phosphate ester and a crosslinking agent. Gelling times and viscosities are improved through the use of an enhancer which is, preferably, an oxyalkylated amine or a blend of an oxyalkylated amine with another amine or a non-nitrogen-containing component. Preferred phosphate esters are ether phosphate esters.

Abstract: The administration of histidine is able to prevent and ameliorate tissue and cellular damage which is caused by damaging levels of cytokines and growth factors. It is shown that histidine, when administered in therapeutic quantities is able to inhibit cytokines and growth factors involved in cell and tissue damage. In addition, the method of adminstering histidine to inhibit these molecules can prevent and ameliorate tissue, vessel and cell damage from restenosis, burns, surgical procedures and other disorders which cause and result from damaged tissues, vessels and cells.

Abstract: A method is provided for the treatment of neoplastic diseases in a mammal which comprises administering to said mammal a therapeutically effective amount of thalidomide. The method also uses a combination of thalidomide with other anti-neoplastic agents. Additionally, pharmaceutical compositions containing thalidomide and other anti-cancer agents are also provided.

Abstract: A composition for the detackification and clarification of acid and alkaline paint and lacquer waste waters and paint spray booth wastes, comprising:an inorganic-organic and/or organic adduct alloy polymer composition having the formula:A.multidot.B.sup.+ .multidot.D.sup.+wherein: A=[(SiO.sub.2 /Me.sup.I.sub.2 O).sub.u Me.sup.II.sub.m Me.sub.m.sup.III(OH).sbsp.p.sup.(SO.sbsp.4.sup.).sbsp.y.sup.(Aci) (2m+3n)-p-2y].sub.rwhere r=1 to 98% bw; u=0 to 10% bw; ##STR1## where: x=0 to 98% bw; Z is a divalent substituted or unsubstituted aliphatic, cycloaliphatic, heterocyclic or aromatic radicalD.sup.+ =(PQAM).sub.wwherew=2 to 98% b.w. of polyquaternized polymer (PQAM)Me.sub.m.sup.II is selected from the divalent cationic group comprising: Mg, Zn, Ca, and Fe.sup.2+m=0 to 5Me.sub.n.sup.III is a tri-or more valent metal selected from the group comprising: Fe, Al, and Al--Zn complexes;n=1 to 20Aci is selected from the monovalent anionic group comprising Cl.sup.-, Br.sup.-, I.sup.-, NO.sub.3 --, H.sub.2 PO.sub.4 --, CH.

Abstract: An innovative liquid mulch method for the production of thin, lightweight and fastly growing sod over an impervious surface such as plastic sheeting are provided. The apparatus of the invention includes, among other components, a transportable agitating tank and a trailing planter assembly that facilitates the liquid mulch method of manufacturing sods.

Abstract: A process for preventing dye redeposition during the washing of a dyed fabric or garment made from natural fibers, which process incorporates as a dye redeposition inhibiting agent, a water-soluble or water-dispersible polyester resin composition comprising a reaction product of 20%-50% by weight of terephthalate polymer or waste terephthalate polymer, 10-40% by weight of at least one glycol and 5-25% by weight of at least one oxyalkylated polyol.

Abstract: The present invention describes site-specific biomolecular complexes comprising a therapeutic, prophylactic and diagnostic agent, called also biologically active molecule and an omega-3 fatty acid and derivatives thereof. The complexes are further covalently bonded with cationic carriers and permeabilizer peptides for delivery across the blood-brain barrier and with targeting moieties for uptake by target brain cells. Said complexes are particularly useful for delivery of a biologically active agent to the glial tissue of the brain as well as to the cortical, cholinergic and adrenergic neurons. The preferred therapeutic complexes or conjugates comprise an omega-3 fatty acid such as alpha-linolenic acid, eicosapentaenoic acid or docosahexaenoic acid and derivatives thereof.