Abstract: The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.

Abstract: This invention is directed to methods for determining a nucleotide sequence of a nucleic acid using positional sequencing by hybridization, and to the creation of nucleic acids probes which may be used with these methods. This invention is also directed to diagnostic aids for analyzing the nucleic acid composition and content of biological samples, including samples derived from medical and agricultural sources.

Abstract: A composite nucleic acid primer for a reaction of an enzymatic extension of primer on a template strand; which composite primer comprises two or more covalently unconnected oligonucleotides. Each of the oligonucleotides comprises a binding segment complementary to a binding site in the template strand. The binding segment and the binding site are selected to bind the oligonucleotide to the template strand by means of annealing between the binding segment and the binding site. The binding sites for different oligonucleotides are selected close enough in the template strand to enhance the sequence specificity of priming by the composite primer, as compared to the sequence specificity of priming by one of the oligonucleotides alone. Each of the two oligonucleotides has a segment, preferably 5 to 6 bases long, binding to the template strand. The total primer-to-template annealing length being 10 to 12 bases, the priming site is sufficiently unique in a plasmid size template.

Abstract: An improved crimping machine for crimping fittings on high pressure flexible hose of the type having a pair of opposed anvils moveable between an uncrimp position and a crimp position. A split crimp cage with each half thereof attached to respective ones of the anvils is provided. The crimp cage retains a plurality of crimping dies axially slideable within corresponding cage slots that are arranged in a uniform circular pattern about the cage. The cage slots extend radially from a common central transverse axis. The improvement comprises the use of crimping dies having substantially the same radial length, and substantially flat anvil cam surfaces that actuate the crimping dies to move at a uniform rate during a crimping operation. To achieve the uniform rate of travel, the flat anvil cam surfaces are oriented at a predetermined angle relative to the corresponding crimping die radial axis.

Abstract: A biflow filter drier has a pair of flapper-type check valve assemblies for directing fluid flow unidirectionally through a centrally supported molded desiccant core. Each check valve assembly is a three part structure spot welded into a unitary assembly comprising a centering support plate, a core support cup and an intermediate flapper valve plate. Two flapper valves are formed from the valve plate and comprise the check valves covering valve openings in the suport plate and support cup. The material which formed the central opening in the support plate is bent at an angle to the support plate and provides an overtravel stop for the check valve. An angled surface of the core support cup provides an overtravel stop for the second check valve.

Abstract: A hydraulic system includes a pump, a reservoir, and an actuator system. A hydraulic circuit breaker is arranged to compare fluid flow to and from the actuator system and to shut off this flow in the event the flow to the actuator system is greater than the flow returning from the actuator system by more than a predetermined differential, thereby indicating a leakage condition. A hydraulic circuit breaker reset device is hydraulically connected to the actuator system and to the circuit breaker. When the circuit breaker is in a shut-off condition, the reset device continuously pressure tests the actuator system. If the pressure in the actuator system increases to indicate the absence of fluid leakage, the reset device responds to the pressure increase in the actuator system to provide a reset signal to the circuit breaker. After the circuit breaker is reset to its normal operating position, a timing piston returns the reset device to its normal operating condition.

Abstract: An extracorporeal medication infusion device provides a precise infusion rate of a liquid medication into a human or animal body. The infusion device includes a permanent portion which has a case, a battery, a programmable electronic controller, and an electromagnetic solenoid core and solenoid coil for providing an actuation force. The infusion device also includes a disposable portion which includes a reservoir filled with a liquid medication that is to be dispensed and a pump for pumping the medication from the reservoir into the user's body. The pump includes a pump chamber and a piston connected to an electromagnetic armature for altering the volume of the pump chamber in response to the actuation force.

Abstract: An extracorporeal medication infusion device provides a precise infusion rate of a liquid medication into a human or animal body. The infusion device includes a permanent portion which has a case, a battery, a programmable electronic controller, and an electromagnetic solenoid core and solenoid coil for providing an actuation force. The infusion device also includes a disposable portion which includes a reservoir filled with a liquid medication that is to be dispensed and a pump for pumping the medication from the reservoir into the user's body. The pump includes a pump chamber and a piston connected to an electromagnetic armature for altering the volume of the pump chamber in response to the actuation force.

Abstract: An extracorporeal medication infusion device provides a precise infusion rate of a liquid medication into a human or animal body. The infusion device includes a permanent portion which has a case, a battery, a programmable electronic controller, and an electromagnetic solenoid core and solenoid coil for providing an actuation force. The infusion device also includes a disposable portion which includes a reservoir filled with a liquid medication that is to be dispensed and a pump for pumping the medication from the reservoir into the user's body. The pump includes a pump chamber and a piston connected to an electromagnetic armature for altering the volume of the pump chamber in response to the actuation force.

Abstract: An extracorporeal medication infusion device provides a precise infusion rate of a liquid medication into a human or animal body. The infusion device includes a permanent portion which has a case, a battery, a programmable electronic controller, and an electromagnetic solenoid core and solenoid coil for providing an actuation force. The infusion device also includes a disposable portion which includes a reservoir filled with a liquid medication that is to be dispensed and a pump for pumping the medication from the reservoir into the user's body. The pump includes a pump chamber and a piston connected to an electromagnetic armature for altering the volume of the pump chamber in response to the actuation force.

Abstract: An electro-hydraulic proportional control valve includes an electro-hydraulic pilot valve, an inlet pressure reducing valve, and a directional control valve. The electro-hydraulic pilot valve receives an electrical input signal and provides a hydraulic signal to the directional control valve in response to the electrical signal. The inlet pressure reducing valve modulates fluid flow from a pump to the directional control valve to maintain a constant pressure drop across the directional control valve under all conditions. The directional control valve includes a directional control valve spool which shifts between a neutral position, a leftward displaced position, and a rightward displaced position in response to the hydraulic signal from the electro-hydraulic pilot valve. The directional control valve spool includes a range adjustment device for adjusting and setting the flow rate through the directional control valve.

Abstract: A sealing sleeve for a rotary type valve is disclosed. The sealing sleeve is formed into a generally circular configuration and is disposed between the housing and the rotor of the valve to provide a firm seal therebetween. Apertures are provided in the periphery of the sleeve and are positioned therein to align with the rotor bores. The inner surface of the sleeve has a thin layer of elastomeric material chemically bonded thereto which terminates in a bead-like configuration about each aperture and on the interior thereof. This bead-like configuration provides a firm seal between the rotor bore and the housing ports eliminating the necessity of O-rings therebetween.

Abstract: A sectional gasket 11 includes corner junction members 12, straight junction members 13, and extension members 14. The junction members 12 and 13 each include a recess 19, and the recesses 19 each include axially extending laterally inwardly facing sealing surfaces 20 and 21. The extension members 14 each have a uniform lateral cross-sectional configuration along their entire axial extent. Each extension member 14 includes axially extending, parallel, laterally outwardly facing sealing surfaces 25 and 26. The sealing surfaces 25 and 26 extend continuously and without interruption along the entire axial extent of the extension member 14. The extension members 14 are characterized by the absence of any special tongue and recess at their opposite ends, and the extension members 14 are manufactured and stored in long continuous strips which are cut to the desired length and used in combination with the junction members 12 and 13 by the end user of the gasket 11.

Abstract: A fluid pressure system includes a variable displacement pump and a control spool. The control spool has one end face exposed to pressure in a control chamber and another end face exposed to pump outlet pressure to increase or decrease the displacement of the pump. The control chamber includes a pilot valve which regulates the maximum pressure in the control chamber. A resistance means and a capacitance means is connected to the control chamber in parallel with the pilot valve to make the control spool responsive to the rate of change of pump outlet pressure.

Abstract: A gas turbine fuel injection nozzle is disclosed in which the secondary fuel is spread into a very thin film entirely within a region of low air momentum. The fuel is therefore not affected by turbulence, and this results in an evenly circumferentially distributed fuel film at the discharge orifice of the nozzle resulting in an even and extremely fine spray of fuel to enhance proper engine performance.

Abstract: A pneumatic circuit includes a source of fluid pressure 11, a selector valve 12, a control valve 14, and a power cylinder 15. The control valve 14 includes main valves 35 and 45 and a pilot interlock valve 27. The main valves 35 and 45 include a main inlet port 36, a main exhaust port 38, and cylinder ports 37 and 47. The pilot interlock valve 27 includes a pilot inlet port 28, an interlock port 30, and a signal port 29 which communicates with signal chambers 39 and 49. The control valve 14 is mounted on a motor vehicle transmission, and a mechanical actuator 16 moves between an extended position when the transmission is out of the neutral position and a retracted position when the transmission is in the neutral position. When the actuator 16 is in the extended position, the pilot interlock valve 27 is retained in an interlocked position to maintain the pressure condition existing in the signal chambers 39 and 49.

Abstract: A fluid control system includes a fluid motor 11, a control valve 12 for directing fluid flow to and from the motor 11, and a control valve actuator 14 for operating the control valve 12. The control valve actuator 14 includes an actuator member 32, a feedback member 44, and a spring 46 which exerts a force on the actuator member 32 whose magnitude varies with the relative positions of the actuator member 32 and the feedback member 44. A command pressure signal moves the actuator member 32 to operate the control valve 12 in order to direct fluid flow to and from the fluid motor 11. A feedback link 41 connects the feedback member 44 to the fluid motor 11. The actuator piston 32 is slidably carried on the feedback member 44, so that any drifting of the fluid motor 11 results in immediate movement of the actuator member 32 and control valve 12 to correct the drifting.

Abstract: A device for adding a second fluid to a first flowing fluid in a fixed ratio regardless of the flow rate of the first fluid. The device comprises a venturi section having an inlet, an outlet and a throat therebetween through which the first fluid flows, and a regulating section that modulates the pressure of the second fluid such that the amount of second fluid delivered to the venturi throat is a fixed proportion of the first fluid flowing through the venturi section regardless of the flow rate of the first fluid. The device has particular application for adding anti-icing fluid to liquid fuel during filling of aircraft fuel tanks.

Abstract: A fuel injection nozzle assembly includes a nozzle housing 11, an inlet connector 12 welded to the housing 11 at a weld joint 14, and a nozzle 15 welded to the housing 11 at a weld joint 16. The weld joint 14, prior to welding, includes a cap 25 having an annular radially outermost crest 26 radially aligned with the joint to be welded. Cap 25 also includes peripheral surfaces 27 and 28 extending axially from the crest 26 in opposite directions and extending radially inwardly from the crest 26 at a slope. The slope is sufficiently steep that an electrical arc 32 between an electrode 31 and the cap 25 will remain on the crest 26 and will not move down the peripheral surfaces 27 and 28 even if the electrode 31 is not precisely aligned with the crest 26.

Abstract: A variable displacement pump 1 includes a housing 2 having an inlet port 3 and an outlet port 4. The pump 1 is an axial piston pump having a swash plate 5 which is actuated to different angular positions by an actuating member 7. The torque limit of the pump 1 is controlled by a horsepower control valve 19 and a speed responsive control valve 35, which change the torque limit of the pump 1 in response to changes in the position of the actuating member 7 and in response to changes in the speed of the engine 33. In this manner, the torque limit of the pump 1 for any position of the actuating member 7 closely approximates the maximum available output torque of the engine 33.