Patents Represented by Attorney Michael W. Krenicky
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Patent number: 8268583Abstract: A progeny cell derived from a parent cell, wherein the progeny cell comprises at least one gene encoding MrgA protein or a functional homologue thereof and/or a DNA segment operably linked with the encoding gene, wherein said gene and/or DNA segment is manipulated with respect to the parent cell; the progeny cell comprises two or more copies of a gene encoding MrgA protein or a functional homologue thereof; or the progeny cell is mutated with respect to the parent cell; whereby the progeny cell produces greater amounts of MrgA protein or a functional homologue thereof than the parent cell.Type: GrantFiled: December 10, 2004Date of Patent: September 18, 2012Assignee: Novozymes A/SInventors: Allan Kent Nielsen, Michael Dolberg Rasmussen
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Patent number: 8258102Abstract: The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X1-X2-X3-X4-X5- where X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is serine or threonine and X5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.Type: GrantFiled: July 23, 2003Date of Patent: September 4, 2012Assignee: Novozymes Biopharma DK A/SInventor: Darrell Sleep
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Patent number: 8252551Abstract: The present invention provides a 2 ?m-family plasmid comprising a polynucleotide sequence insertion, deletion and/or substitution between the first base after the last functional codon of at least one of either a REP2 gene or an FLP gene and the last base before the FRT site in an inverted repeat adjacent to said gene.Type: GrantFiled: December 23, 2004Date of Patent: August 28, 2012Assignee: Novozymes Biopharma DK A/SInventors: Darrell Sleep, Christopher John Arthur Finnis
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Patent number: 8252573Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: February 3, 2011Date of Patent: August 28, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Patent number: 8236528Abstract: A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.Type: GrantFiled: February 15, 2012Date of Patent: August 7, 2012Assignee: Novozymes A/SInventors: Shinobu Takagi, Noriko Tsutsumi, Yuji Terui, XiangYu Kong
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Patent number: 8227227Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: GrantFiled: November 30, 2007Date of Patent: July 24, 2012Assignee: Novozymes A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson
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Patent number: 8143023Abstract: A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.Type: GrantFiled: January 25, 2008Date of Patent: March 27, 2012Assignee: Novozymes A/SInventors: Shinobu Takagi, Noriko Tsutsumi, Yuji Terui, XiangYu Kong
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Patent number: 8124379Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: GrantFiled: March 31, 2009Date of Patent: February 28, 2012Assignee: Novozymes A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Patent number: 8119387Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.Type: GrantFiled: August 15, 2006Date of Patent: February 21, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Reinhard Wilting, Martin Borchert
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Patent number: 8088598Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.Type: GrantFiled: December 5, 2003Date of Patent: January 3, 2012Assignee: Novozymes A/SInventors: Carsten M. Hjort, C. A. M. J. J. Van Den Hondel, P. J. Punt, F. H. J. Schuren, Tove Christensen
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Patent number: 8071345Abstract: The present invention relates to a liquid detergent composition comprising a surfactant, a subtilisin and a protease stabilizer that is 3-chlorobenzoic acid, 4-chlorobenzoic acid, 3-chlorophenylacetic acid, 3,5-dichlorobenzoic acid, 3-(3-chlorophenyl)propionic acid or their corresponding salts. The composition can further comprise a another enzyme that is a lipase, an amylase, a cellulase or mixtures thereof. The stabilizer can be present at a concentration of 0.001 to 20% w/w. The concentration of the subtilisin can be at least 1.5 g/L.Type: GrantFiled: March 29, 2007Date of Patent: December 6, 2011Assignee: Novozymes A/SInventors: Lone Kierstein Nielsen, Ole Simonsen, Karl Werntoft, Niclas Ilestam
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Patent number: 8017351Abstract: The pharmaceutical use of amylases related to Bacillus alpha-amylases of SEQ ID NOs: 1-3, optionally in combination with a lipase and/or a protease. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The amylases of SEQ ID NOs: 1-3 are variants of amylases from Bacillus stearothermophilus, Bacillus licheniformis and Bacillus sp. The amylases of the invention have an improved efficacy in vivo, an improved pH-profile, a high specific activity, and/or an improved starch degradation profile.Type: GrantFiled: June 16, 2006Date of Patent: September 13, 2011Assignees: Novozymes A/S, Solvay Pharmaceuticals GmbHInventors: Allan Svendsen, Peter Collin Gregory
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Patent number: 7960332Abstract: A coated granule including a core. The core includes a uniform mixture of a detergent enzyme having an alkaline pH activity optimum, and an acidic buffer component. The acidic buffer component has a pH of 1 to below 7 when measured as a 10% aqueous solution with a pKa in the range of 4 to 9.Type: GrantFiled: January 26, 2004Date of Patent: June 14, 2011Assignee: Novozymes A/SInventors: Ole Simonsen, Erik Kjaer Markussen, Hanne Philbert Nielsen, Lone Aslaug Hansen
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Patent number: 7951556Abstract: A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides.Type: GrantFiled: April 7, 2005Date of Patent: May 31, 2011Assignee: Novozymes A/SInventors: Allan Kent Nielsen, Michael Dolberg Rasmussen
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Patent number: 7951267Abstract: The invention relates to the use of certain lipolytic enzymes such as cutinases and lipases in the manufacture of paper and paper products from recycled paper. Examples of such enzymes are derived from strains of Humicola, Candida, Fusarium and Pseudomonas. By use of these enzymes, the problems relating to the so-called “stickies” derived from waste paper are reduced.Type: GrantFiled: January 23, 2007Date of Patent: May 31, 2011Inventors: Kim Borch, Henrik Lund, Masaki Sharyo, Hiromichi Sakaguchi, Hanne Host Pedersen, James William Fitzhenry
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Patent number: 7919275Abstract: An isolated mutant host cell produced by disrupting or deleting, in a parent cell, a nucleic acid sequence encoding an oxaloacetate hydrolase, or a promoter thereof, which results in the mutant cell producing less of the oxaloacetate hydrolase than the parent cell is disclosed. The disclosure also relates to recombinant methods for producing the products such as polypeptides.Type: GrantFiled: March 8, 2010Date of Patent: April 5, 2011Assignee: Novozymes A/SInventors: Carsten Mailand Hjort, Henrik Pedersen, Jens Nielsen
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Patent number: 7892814Abstract: The present invention relates to use of heat-stable carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas or biogas. Furthermore, the invention relates to isolated polypeptides having carbonic anhydrase activity at elevated temperatures and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides.Type: GrantFiled: August 3, 2010Date of Patent: February 22, 2011Assignee: Novozymes A/SInventors: Martin Borchert, Paria Saunders
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Patent number: 7858360Abstract: The disclosure relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity, an endogenous serine protease activity, and an endogenous kexin maturase activity have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous.neutral metalloprotease activity, the endogenous serine protease activity, and the kexin maturase activity are encoded by the alp, npI, pepC and kexB genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies.Type: GrantFiled: October 17, 2006Date of Patent: December 28, 2010Assignee: Novozymes A/SInventors: Mogens Trier Hansen, Christian Isak Jorgensen, Jan Lehmbeck
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Patent number: 7855059Abstract: A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.Type: GrantFiled: June 4, 2002Date of Patent: December 21, 2010Assignee: Novozymes A/SInventors: Kevin S. Wenger, Maria Antonieta Caicedo, Stuart Michael Stocks, Swapnil Bhargava, Mark R. Marten
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Patent number: 7803575Abstract: The present invention relates to use of heat-stable carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas or biogas. Furthermore, the invention relates to isolated polypeptides having carbonic anhydrase activity at elevated temperatures and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides.Type: GrantFiled: January 31, 2008Date of Patent: September 28, 2010Assignee: Novozymes A/SInventors: Martin Borchert, Paria Saunders