Patents Represented by Attorney Michael W. Krenicky
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Patent number: 7794997Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: GrantFiled: December 10, 2009Date of Patent: September 14, 2010Assignee: Novozymes A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Patent number: 7700338Abstract: The invention relates to isolated CGTases and isolated nucleic acid sequences encoding the CGTases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the CGTases, in particular to cleaning and detergent composition comprising such CGTases.Type: GrantFiled: July 13, 2001Date of Patent: April 20, 2010Assignee: Novozymes A/SInventors: Per Lina Jorgensen, Claus Crone Fuglsang
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Patent number: 7700322Abstract: A bacterial host cell comprising at least two copies of an amplification unit in its genome, said amplification unit comprising: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of said conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: GrantFiled: October 29, 2004Date of Patent: April 20, 2010Assignee: Novozymes A/SInventors: Carsten Olsen, Michael Dolberg Rasmussen
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Patent number: 7659101Abstract: This invention relates to an amylase derived from Bacillus horikoshii. The amylase has a preference for soluble substrates, shows substantial exo-amylase activity and has a pH optimum of about 9.8.Type: GrantFiled: December 15, 2005Date of Patent: February 9, 2010Assignee: Novozymes A/SInventors: Tine Hoff, Shamkant Anant Patkar, Jeppe Wegener Tams
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Patent number: 7632799Abstract: The present invention relates to a process for preparing coated granules comprising the steps of: (a) providing a core unit comprising an active component (b) contacting the core unit with a liquid dispersion comprising a solvent, a dissolved salt and solid dispersed particles wherein the solid particles constitute at least 10% w/w of the total dry matter of the dispersion (c) evaporating the solvent of the liquid dispersion to leave salt and solid particles coated onto the core unit.Type: GrantFiled: May 26, 2006Date of Patent: December 15, 2009Assignee: Novozymes A/SInventors: Poul Bach, Ole Simonsen
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Patent number: 7625737Abstract: The present invention relates to alpha-amylase variants having mutations corresponding to one or more of the following mutations A181E,D,Q,N,V; I201W,F,L; Q264R,D,K,E; Y290R,E,K; and S334A,V,T,L,I,D (using SEQ ID No. 2 for numbering).Type: GrantFiled: November 3, 2004Date of Patent: December 1, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Patent number: 7601527Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an ?-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an ?-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an ?-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an ?-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like ?-amylase, which variant exhibits increased.Type: GrantFiled: January 19, 2005Date of Patent: October 13, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Sóeren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Patent number: 7566561Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: December 23, 2002Date of Patent: July 28, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 7534762Abstract: The present invention relates to isolated polypeptides having antimicrobial activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: March 20, 2006Date of Patent: May 19, 2009Assignee: Novozymes A/SInventors: Kirk Matthew Schnorr, Nikolaj Spodsberg
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Patent number: 7527947Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: GrantFiled: June 14, 2005Date of Patent: May 5, 2009Assignee: Novozymes A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Patent number: 7521204Abstract: A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.Type: GrantFiled: March 25, 2003Date of Patent: April 21, 2009Assignee: Novozymes A/SInventors: Jens Tonne Andersen, Steen Troels Jorgensen, Michael Dolbjerg Rasmussen, Peter Bjarke Olsen, Ib Groth Clausen
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Patent number: 7517668Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.Type: GrantFiled: May 5, 1995Date of Patent: April 14, 2009Assignee: Novozymes A/SInventors: Esper Boel, Tove Christensen, Helle Fabricius Wöldike
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Patent number: 7498158Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: May 15, 2002Date of Patent: March 3, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Patent number: 7485447Abstract: A secreted proteolytic polypeptide comprising at least three non-polar or uncharged polar amino acids within the last four amino acids of the C-terminus of the polypeptide, encoding polynucleotides, expression vectors comprising the polynucleotides, host cell comprising the polynucleotides, methods for producing said polypeptide, and methods for using the polypeptide.Type: GrantFiled: June 21, 2004Date of Patent: February 3, 2009Assignee: Novozymes A/SInventor: Soren Flensted Lassen
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Patent number: 7445918Abstract: The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern.Type: GrantFiled: June 28, 2004Date of Patent: November 4, 2008Assignee: Novozymes A/SInventor: Allan Svendsen
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Patent number: 7425528Abstract: The present invention relates to granules comprising a core matrix and one or more coatings, wherein the core matrix comprises: An active compound, a synthetic polymer, where the amount of polymer added is 0.1 to 10% by weight of the matrix and one or more antioxidants and/or reducing agents, where the amount of antioxidant and/or reducing agent is 0.2 to 5% by weight of the matrix.Type: GrantFiled: June 30, 2003Date of Patent: September 16, 2008Assignee: Novozymes A/SInventors: Ole Simonsen, Erik Kjaer Markussen, Hanne Rojel, Svend Kaasgaard, Thomas Honger Callisen, Christian Isak Jorgensen
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Patent number: 7419947Abstract: The present invention relates to a granule comprising a core and a coating, wherein the core comprises an active compound, and the coating comprises filaments prepared from atomizing a liquid coating composition having the property that liquid coating composition forms filaments upon atomization.Type: GrantFiled: March 26, 2003Date of Patent: September 2, 2008Assignee: Novozymes A/SInventors: Poul Bach, Ole Simonsen
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Patent number: 7374922Abstract: The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.Type: GrantFiled: February 26, 2003Date of Patent: May 20, 2008Assignee: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen
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Patent number: 7371546Abstract: The invention relates to a variant of a parental fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: GrantFiled: September 27, 2002Date of Patent: May 13, 2008Assignee: Novozymes A/SInventor: Allan Svendsen
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Patent number: 7319087Abstract: The present invention relates to an anti-microbial polypeptide and a DNA construct encoding said anti-microbial polypeptide and the use of said anti-microbial polypeptide.Type: GrantFiled: May 3, 2002Date of Patent: January 15, 2008Assignee: Novozymes A/SInventor: Morgens Trier Hansen