Abstract: Particular enzymes are precipitated from a mixture of proteins by the simultaneous addition of a soluble aluminate and acid. The pH of the mixture is at least one pH unit from the isoelectric point of the particular enzyme.

Abstract: An enzyme-containing granulate is prepared containing a core and a shell wherein the core and/or shell contain an enzyme and the shell contains artificial or cellulose fibers in an amount of 1.5-40%. The core may also contain the fibers in an amount of 1.5-40%. In a preferred embodiment, the core contains a primary enzyme, the fibers, a coating of a sustained release agent, and the shell contains a secondary enzyme and the fibers. The sustained release coating causes the primary enzyme to be released more slowly than the secondary enzyme in a washing solution. In another embodiment, the core contains a primary detergent additive, a coating of a protective agent, and the shell contains a secondary detergent additive and the fibers. The protective coating separates the primary and secondary detergent additives so they do not harm each other during storage. Preferably, the core and shell also contain a binder, a filler and a granulating agent.

Abstract: The present invention relates to a method of separating a protein, in particular an enzyme, from an aqueous solution of proteins, comprising (a) providing an aqueous mixture of proteins with a salt concentration at or below 1.5 Molar, to which a water soluble polymer has been added, and (b) recovery of the protein on crystalline form.

Abstract: A DNA construct comprising the following sequence: 5'-P-SP-(LP).sub.n -PS-HP-3' wherein P is a promoter sequence, SP is a DNA sequence encoding the yeast aspartic protease 3 (YAP3) signal peptide, LP is a DNA sequence encoding a leader peptide, n is 0 or 1, PS is a DNA sequence encoding a peptide defining a yeast processing site, and HP is a DNA sequence encoding a polypeptide which is heterologous to a selected host organism. The YAP3 signal peptide provides efficient secretion of heterologous proteins in yeast.

Abstract: An enzyme from Aspergillus aculeatus exhibiting endoglucanase activity, which enzyme is designated EG II or EG IV and is encoded by a DNA sequence comprising at least one of the partial sequences shown in SEQ ID NO: 17, 18, or 19. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell wall material.

Abstract: The present invention relates to a method of separating an enzyme from an aqueous solution comprising this enzyme in mixture with other proteins, and recovery of the desired enzyme on crystalline form.

Abstract: A well tasting and organoleptically acceptable vegetable protein hydrolyzate such as soy, pea or rice protein hydrolyzate is produced in high yield by a method using a combination of non-pH-stat hydrolysis and ultrafiltration. Preferably, the method is carried out by mixing a material containing at least 65% vegetable protein as dry matter and water to form a slurry containing a vegetable protein content of about 7-20%, heating the slurry to above 60.degree. C., adjusting the pH of the slurry to about 8.5, hydrolyzing the slurry with at least two different proteases to a degree of hydrolysis of between 15 and 35% without adjusting the pH during hydrolysis to produce a hydrolyzed slurry, inactivating the proteases and separating the hydrolyzed slurry with an ultrafiltration unit having a cut-off value above 5,000 to form a permeate containing the vegetable protein hydrolyzate. One protease may be obtained from B. Licheniformis and the other from B. Subtilis.

Abstract: The present invention relates to certain sterol derivatives for use as medicaments.

Abstract: The present invention relates to Fervidobacterium amylase and pullulanase preparations and their use in producing sweeteners and ethanol from starch. In particular the enzymes are derived from Fervidobacterium pennavorans.

Abstract: The transfer of a textile dye from a dyed fabric to another fabric during washing or rinsing is inhibited by adding an enzyme exhibiting peroxidase activity or an enzyme exhibiting a suitable oxidase activity to the wash liquor in which said fabrics are washed and/or rinsed. Peroxidase is produced extracellularly by some strains of Bacillus pumilus. The novel peroxidase preparation from B. pumilus is a microperoxidase, i.e. it contains hemopeptide as an active component. The preparation has improved stability at high temperature, at high pH and at high concentrations of hydrogen peroxide. It can be produced without undesired catalase activity.

Abstract: An enzyme exhibiting pectin methyl esterase activity, wherein the enzyme a) is derived from Aspergillus aculeatus; b) is encoded by Seq ID No:1; or c) has the amino acid sequence of Seq ID No:2 or a sequence which is at least 95% homologous thereto. An enzyme can be used for the modification of plant cell wall components.

Abstract: The transfer of a textile dye from a dyed fabric to another fabric during washing or rinsing is inhibited by adding an enzyme exhibiting peroxidase activity or an enzyme exhibiting a suitable oxidase activity to the wash liquor in which said fabrics are washed and/or rinsed. Peroxidase is produced extracellularly by some strains of Bacillus pumilus. The novel peroxidase preparation from B. pumilus is a microperoxidase, i.e. it contains hemopeptide as an active component. The preparation has improved stability at high temperature, at high pH and at high concentrations of hydrogen peroxide. It can be produced without undesired catalase activity.

Abstract: This invention relates to activation of enzymes. More specifically, the invention relates to peroxidase enhancing agents. The invention also relates to methods of oxidizing a substrate with a source of hydrogen peroxide in the presence of a peroxidase enzyme and a peroxidase enhancing agent More specifically, the invention relates to a method of bleaching of dye in solutions, to a method of inhibiting the transfer of a textile dye from a dyed fabric to another fabric when said fabrics are washed together in a wash liquor, to a method of bleaching of lignin-containing material in particular bleaching of pulp for paper production, to a method of treatment of waste water from pulp manufacturing, and to a method of enzymatic polymerization and/or modification of lignin or lignin containing material.

Abstract: A Bacillus promoter included in DNA sequence (SEQ ID#1), wherein each of N.sup.1 -N.sup.9 is A, T, C or G with the exception that N.sup.2 -N.sup.9 do not together form the sequence ATGTTTCA or GTGTTTCA, or a functional homologue of said sequence.

Abstract: The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.

Abstract: An active recombinant trypsin-like protease enzyme comprising the amino acid residues 25-224 of the amino acid sequence of SEQ ID NO:2, as well as a DNA construct encoding the enzyme and comprising the sequence of SEQ ID NO:1. The enzyme may be used as a detergent enzyme.

Abstract: An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspercgillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components, e.g., in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.

Abstract: The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.

Abstract: A well tasting and organoleptically acceptable whey protein hydrolyzate is produced in high yield by a method using a combination of non-pH-stat hydrolysis and ultrafiltration. Preferably, the method is carried out by mixing a material containing at least 65% whey protein as dry matter and water to form a slurry containing a whey protein content of about 7-20%, heating the slurry to above 600.degree. C., adjusting the pH of the slurry to about 8, hydrolyzing the slurry with at least two different proteases to a degree of hydrolysis of between 17 and 35% without adjusting the pH during hydrolysis to produce a hydrolyzed slurry, inactivating the proteases and separating the hydrolyzed slurry with an ultrafiltration unit having a cut-off value above 10,000 to form a permeate containing the whey protein hydrolyzate. One protease may be obtained from B. Licheniformis and the other from B. Subtilis.

Abstract: The present invention relates to a xylanase from the family Desulfurococcaceae, specifically to a xylanase from the genus Pyrodictium and more specifically to a xylanase from Pyrodictium abyssi and in particular to a xylanase from Pyrodictium abyssi, DSM 6158, which has (a) activity optimum in the pH range 5.5-6.5 at 100.degree. C. with xylan as a substrate and (b) activity optimum at the temperature range 105.degree.-115.degree. C., determined at pH 5.5 with xylan as a substrate. The present invention also relates to a process of bleaching lignocellulosic pulp with said xylanase.