Abstract: The present invention relates to an expression system which comprises a phage-like bacteriocin("phibacin") or a mutant thereof, or a gene or mutant of a phibacin having a function in gene expression, such as a repressor gene, which is used to transform bacterial host for the producion of proteins, in particular in gram positive bacteria.

Abstract: The present invention relates to insulin crystals comprising ASP.sup.B28 and protamine, and pharmaceutical preparations containing same. The crystals and preparations exhibit rapid onset and prolonged activity when administered in vivo.

Abstract: A method for rapidly and inexpensively crystallizing enzymes from an impure mixture is disclosed. The yield of the enzyme is greater than 35% within twelve hours. The crystallizing agent is a salt added in a concentration which is less than the concentration necessary to crystallize the enzyme in amorphous form.

Abstract: The present invention relates to insulin crystals comprising ASP.sup.B28 and protamine, and pharmaceutical preparations containing same, The crystals and preparations exhibit rapid onset and prolonged activity when administered in vivo.

Abstract: The present invention relates to the use of certain chemical compounds which interfere with the biosynthesis of cholesterol and medicaments comprising such compounds for stimulating the meiosis of oocytes and spermatozoon in vivo, ex vivo and in vitro.

Abstract: The invention relates, inter alia, to a DNA construct comprising a DNA sequence which encodes an enzyme exhibiting lipolytic activity and which comprises: a) the DNA sequence shown in SEQ ID No. 1, or b) an analogue of the DNA sequence shown in SEQ ID No. 1 which i) is homologous with the DNA sequence shown in SEQ ID No. 1, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 1, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or which is derived from Humicola insolens DSM 1800. Also disclosed, inter alia, are: lipolytic enzymes encoded by such DNA constructs; and enzyme preparations, detergent additives and detergent compositions comprising such lipolytic enzymes.

Abstract: A DNA sequence encoding insulin receptor substrate 1 (IRS-1), the DNA sequence containing a mutation of at least one nucleotide, and the protein encoded by said DNA sequence.

Abstract: Bacteria belonging to the genus Pseudomonas, alkaline lipase produced by the bacteria and having the following properties, a method of producing the lipase, and detergent compositions containing the lipase:(1) Operating pH and optimum pHan operating pH is in the range of from 3.5 to 12 and an optimum pH is in the range of from 10 to 11 using a triolein emulsion as a substrate;(2) Operating temperature and optimum temperaturean operating temperature is in the range of from 30.degree. C. to 80.degree. C. and an optimum temperature is in the range of from 55.degree. C. to 65.degree. C. using the triolein emulsion as a substrate;(3) Molecular weighta molecular weight measured by SDS-polyacrylamide gel electrophoresis is 31,000.+-.2,000; and(4) Isoelectric pointan Isoelectric point measured by isoelectric point polyacrylamide gel electrophoresis is 5.2.+-.0.5.The lipase has high stability against detergent components such as surfactants, protease, etc. and can be blended together with protease with detergents.

Abstract: DNA encoding an endoglucanase from Trichoderma harzianum is disclosed. The endoglucanase has activity toward mixed .beta.-1,3-1,4 glucans and is especially useful in brewing processes.

Abstract: The present invention relates to animal feed additives, which additives comprise a monocomponent xylanase derived from a strain of Byssochlamus, Chaetomium, Humicola, Malbranchea, Mucor, Myceliophthora, Paecilomyces, Talaromyces, Thermoascus, or Thielavia. In other aspects, the invention relates to monocomponent xylanase preparations, DNA constructs, recombinant expression vectors, host cells, and methods of producing monocomponent xylanase preparations.

Abstract: The present invention relates to a novel variant of peroxidase with improved stability at alkaline conditions, and a bleaching or detergent composition comprising the peroxidase variant.

Abstract: An enzyme exhibiting mannanase activity, which enzyme i) is immunologically reactive with an antibody raised against a purified mannanase derived from Aspergillus aculeatus, CBS 101.43; ii) is encoded by the DNA sequences shown in SEQ ID No. 1 or an analogue of said sequence, and/or; iii) comprises the amino acid sequence shown in SEQ ID No. 2 or a sequence being an least 70% homologous thereto. The enzyme may be used for various purposes for which degradation or modification of a plant or algal cell wall material is desirable.

Abstract: A cellulase variant of a parent cellulase, e.g. a cellulase classified in family 45 such as a Humicola insolens 43 kD endoglucanase, comprising a cellulose binding domain (CBD), a catalytically active domain (CAD) and a region linking the cellulose binding domain and catalytically active domain (the linking region), wherein one more amino acid residues of the CBD, CAD or linking region is deleted or substituted by one or more amino acid residues and/or one or more amino acids are added to the linking region and/or another CBD is added at the opposite end of the catalytically active domain, has improved properties as regards e.g. alkaline activity, compatibility with detergent composition ingredients, particulate soil removal, color clarification, defuzzing, depilling, harshness reduction, and sensitivity to anionic surfactants and peroxidase bleaching systems and is useful e.g. in detergent compositions, for textile treatment, in paper pulp processing, for animal feed and for stone washing of jeans.

Abstract: A human spasmolytic polypeptide (HSP) having the amino acid of SEQ ID NO:1, characterized by being in a glycosylated form.

Abstract: A method of producing chemically stable and biologically active growth hormone crystals and processes for production of pharmaceutical preparations containing these growth hormone crystals.

Abstract: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71.

Abstract: The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.

Abstract: The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.

Abstract: The present invention relates to methods for producing heterologous heme proteins extracellularly comprising transforming a filamentous fungus with a vector comprising a DNA sequence encoding the heterologous heme protein and a DNA sequence encoding a preregion permitting secretion of the expressed heme protein, and culturing the transformed filamentous fungus in a suitable culture medium to produce the heme protein.

Abstract: Disclosed is a process for chemical finishing of fabrics, fibers or yarns wherein insoluble cellulosic polymers are reacted with carboxylic acids or esters thereof in the presence of a lipase. The cellulosic polymer may be cotton, viscose, rayon, lyocell, flax, linen, ramie, and all blends thereof; and blends thereof with polyesters, wool, polyamides, acrylics and polyacrylics. The lipase may be a microbial lipase, including a lipase obtained from yeast, e.g. Candida lipase, a bacterial lipase, e.g. Pseudomonas lipase, or a fungal lipase, e.g. Humicola or Rhizomucor lipases. Chemically modified lipases obtained by coupling polyethylene glycol to amino acid residues of the lipase may also be used.