Abstract: Methods and systems for synthesizing an oligonucleotide in a reaction chamber comprising a solid support. A nucleotide monomer is selected for addition to an oligonucleotide. An inlet stream comprising the selected monomer is fed to the reaction chamber (column) of the reaction chamber and conditions are provided in the reaction chamber for oligonucleotide synthesis. An outlet stream from the reaction chamber can be split and directed to the waste drain and/or to an analysis station where the eluent is analyzed by mass spectrometry (MS) to confirm the identity of the monomer fed through the inlet to the reaction chamber. Based on results from analyzing by MS, it is determined whether the outlet stream comprises the selected nucleotide monomer or a derivative thereof.

Abstract: A spectrometry system for spectroscopically analyzing a sample is provided. The system includes an excitation source for interacting with the sample; a detector for detecting at least a portion of light absorbed or emitted by the sample, the excitation source and detector being optically coupled via an optical pathway; and an aperture positioned in the optical pathway for limiting transmission of light from the excitation source to the detector; wherein the aperture is configured to have a spatially varying distribution of one or more geometric features that provide regions of variable transmission around an edge of the aperture. Also provided is a mask for use with a spectrometry system, the mask configured to be positioned in an optical pathway between an excitation source and a detector, wherein the mask has a spatially varying distribution of one or more geometric features that provide regions of variable transmission around an edge of the aperture.

Abstract: A shaft coupling system may include a first shaft including a disc that is engageable by a second shaft to retain the second shaft with the first shaft when the second shaft is rotated in a first direction, and release the second shaft from the first shaft when the second shaft is rotated in a second direction that is opposite to the first direction. A spring may be mounted to the first shaft to bias the second shaft towards the first shaft when the second shaft is retained with the first shaft. Further, a shaft coupling method may include engaging a second shaft with a disc of a first shaft by rotating the second shaft in a first direction relative to the first shaft, and disengaging the second shaft from the first shaft by rotating the second shaft in a second direction relative to the first shaft.

Abstract: A temperature control chamber includes a heat impact unit configured for heat impacting a sensor unit in accordance with a heat profile, the sensor unit configured for sensing heat impact response data over time in response to being heat impacted with the heat profile, and a determining unit configured for determining information indicative of a heat transfer characteristic in the temperature control chamber based on the sensed heat impact response data.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring carrier may be operatively disposed between the screw drive and the screw, and movable along an axis of the spring carrier by the screw drive to impart, by the screw, a sealing axial force to seal a capillary tip of a capillary against a port ground of the port.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring-loaded disc may be operatively disposed between the screw drive and the screw, and rotatable by the screw drive to impart, by the screw, a sealing axial force to seal a capillary tip of a capillary against a port ground of the port.

Abstract: In some examples, a capillary connection fitting may include a fitting housing that encloses a socket retainer and a spring. A spring compression limiter may include a specified length and is attached to the socket retainer to limit compression of the spring upon movement of the socket retainer to compress the spring.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring-loaded disc may be operatively disposed between the screw drive and the screw, and axially movable by a socket of a capillary to impart, by the spring-loaded disc, a sealing axial force to seal a capillary tip of the capillary against a port ground of the port.

Abstract: In alternative embodiments, provided are immunohistochemistry (IHC) methods and kits for determining and scoring reproducibly the extent of expression of the protein tyrosine-protein kinase-like 7 (PTK7), also known as: colon carcinoma kinase 4 (CCK4); HER2 or receptor tyrosine-protein kinase erbB-2, or cluster of differentiation 340 (CD340); programmed death-ligand 1 (PD-L1), or cluster of differentiation 274 (CD274); B7 homolog 1 (B7-H1); and, Ki-67 or MKI67 (Marker of Proliferation Ki-67), in a tissue sample. In alternative embodiments, provided are methods and kits for diagnosing or selecting an individual eligible for treatment with a cancer or tumor therapeutic, or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein.

Abstract: Disclosed herein are methods and systems for evaluating the bioenergetic poise and bioenergetic capacity of living cells in a single assay.

Abstract: An apparatus having a waveguide and a freeform collimating lens. The waveguide is characterized by a waveguide axis and a planar end having a normal axis that is inclined at an end angle that is greater than 0 degrees with respect to the waveguide axis. The freeform collimating lens collimates light leaving the planar end of the waveguide into a collimated light beam characterized by a beam direction that is parallel to the waveguide axis. The apparatus inhibits reflections from the planar end of the waveguide from propagating back down the waveguide while providing a collimated light beam having a direction parallel to the axis of the waveguide.

Abstract: A sampling unit is fluidically coupled to a source. The sampling unit is configured for receiving a fluidic sample from the source and chromatographically separating the received fluidic sample. The sampling unit is operated to introduce a test sample into the source. At least a portion of the introduced test sample from the source is received into the sampling unit, and the received portion of the test sample is chromatographically separated in the sampling unit.

Abstract: In some examples, an apparatus may include analyzing a sample, and analyzing at least one reference sample. A determination may be made as to whether the sample includes a library match score. Based on a determination that the sample includes the library match score, the library match score and a retention time may be analyzed to determine similarity between the sample and the at least one reference sample.

Abstract: There is provided a method for training a ground truth generator machine learning model, comprising: creating a ground truth multi-record training dataset wherein a record comprises: a first image of a sample of tissue of a subject depicting a first group of biological objects, a second image of the sample depicting a second group of biological objects presenting at least one biomarker, and ground truth labels indicating a respective biological object category of a plurality of biological object categories for biological object members of the first group and the second group; and training the ground truth generator machine learning model on the ground truth multi-record training dataset for automatically generating ground truth labels selected from the plurality of biological object categories for biological objects depicted in an input set of images of a first type corresponding to the first image and a second type corresponding to the second image.

Abstract: A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve to transfer the sample loop between a low pressure state and a high pressure state via an intermediate state and for controlling the metering device during the intermediate state to at least partially equilibrate a pressure difference in the sample loop between the low pressure state and the high pressure state.

Abstract: A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve to transfer the sample loop between a low pressure state and a high pressure state via an intermediate state and for controlling the metering device during the intermediate state to at least partially equilibrate a pressure difference in the sample loop between the low pressure state and the high pressure state.

Abstract: Compounds useful for forming nucleic acids having the structure of Formula I: Each of R1 or R2 is independently selected from hydrogen, a protecting group, or a phosphoramidite group. R3 is selected from H, F, O—C1-6 alkyl, O-MOE and a removable hydroxyl-protecting group. Q is a heterocyclic base. R4 is a cyclic hydrocarbon. Also disclosed are processes for forming the nucleic acids from the compounds and the nucleic acid products produced.

Abstract: In some examples, deterring a third party from copying a product or process may include scanning code to generate scanned code. Text in the scanned code may be read and/or at least one image in the scanned code may be recognized. The text and/or the at least one image may be analyzed to determine whether the code includes a specified trademark. Based on a determination that the code includes the specified trademark, an indication of authenticity of the code may be generated.

Abstract: In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.

Abstract: In some examples, linear and non-linear range-based plot pane selection may include receiving data that is to be displayed. Based on an increase or a decrease in a size of a range selector, a selection of a range of plot panes may be received from a plurality of available plot panes to display the data. Based on the received selection of the range of plot panes, a display of the data may be generated in plot panes included in the range of plot panes. The size of the range selector may be non-linearly proportional to the available plot panes to display the data.