Abstract: A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.

Abstract: In some examples, an ion funnel-based collision cell may include an ion funnel entrance section formed by a plurality of adjacently disposed entrance members. Each entrance member of at least one pair of the adjacently disposed entrance members may include a successively larger opening to form a tapered or profiled entrance for ions entering the ion funnel-based collision cell. An insulation material may be disposed adjacent to or in contact with each entrance member of the at least one pair of the adjacently disposed entrance members to prevent, outside of each successively larger opening, flow of gas between each entrance member of the at least one pair of the adjacently disposed entrance members.

Abstract: In some examples, deterring a third party from copying a product or process may include scanning code to generate scanned code. Text in the scanned code may be read and/or at least one image in the scanned code may be recognized. The text and/or the at least one image may be analyzed to determine whether the code includes a specified trademark. Based on a determination that the code includes the specified trademark, an indication of authenticity of the code may be generated.

Abstract: An electrostatic lens for transporting charged particles in an axial direction includes a first group of first electrodes configured to receive a first DC potential from a DC voltage source, and a second group of second electrodes configured to receive a second DC potential from the DC voltage source different from the first DC potential. The first electrodes are interdigitated with the second electrodes. The first group and/or the second group has a geometric feature that progressively varies along the axial direction. The lens generates an axial potential profile that progressively changes along the axial direction, and thereby reduces geometrical aberrations. The lens may be part of a charged particle processing apparatus such as, for example, a mass spectrometer or an electron microscope.

Abstract: In some examples, a multipole section-based ion funnel may include an ion funnel section formed by at least one pair of adjacently disposed members. A first member of the at least one pair of adjacently disposed members may include a pole structure. A second member of the at least one pair of adjacently disposed members may include a pole structure that is engageable with the pole structure of the first member to form a multipole structure.

Abstract: Provided herein are compositions and methods for inducing CRISPR/Cas-based editing of a target nucleic acid (e.g., target DNA or target RNA) in vitro or in a cell, using modified prime editing guide RNAs (pegRNAs) that incorporate one or more chemically-modified nucleotides. The modified pegRNAs disclosed herein may be used to induce Cas-mediated incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target nucleic acid. The nucleotide change can include, e.g., one or more nucleotide changes, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.

Abstract: The invention provides an optical coupling, comprising: a first coupling portion and a second coupling portion, the first and second coupling portions configured to mate in kinematic constraint via a first mounting interface and at least two further mounting interfaces, wherein: the first coupling portion comprises a first mounting element and the second coupling portion comprises a socket, and the first mounting element is receivable in the socket at the first mounting interface such that the first mounting interface constrains lateral displacements of the first coupling portion relative to the second coupling portion when mated; and at least one optical channel for transmitting abeam of light along an optical axis through the optical coupling, the optical channel comprising complementary light-transmitting ports in the first and second coupling portions, wherein the optical axis is not laterally removed from the first mounting interface by more than half the distance between the first mounting interface and

Abstract: Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.

Abstract: An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample including a confocal imaging subsystem, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

Abstract: Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.

Abstract: In some examples, a load adapter may include a body including an elongated shaft that includes an inlet passage that is in fluid communication with at least one outlet hole. The at least one outlet hole may be dimensioned to provide an opening area that is between approximately 5% to approximately 90% of a face of the body.

Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Abstract: Novel tools and techniques are provided for implementing augmented reality (AR)-based assistance within a work environment. In various embodiments, a computing system might receive, from a camera having a field of view of a work environment, first images of at least part of the work environment, the first images overlapping with a field of view of a user wearing an AR headset; might analyze the received first images to identify objects; might query a database(s) to determine a task associated with a first object(s) among the identified objects; might generate an image overlay providing at least one of graphical icon-based, text-based, image-based, and/or highlighting-based instruction(s) each indicative of instructions presented to the user to implement the task associated with the first object(s); and might display, to the user's eyes through the AR headset, the generated first image overlay that overlaps with the field of view of the user's eyes.

Abstract: A device, including a hydrophilic layer on a portion of an inner surface of a transparent polymer forming a body; wherein the hydrophilic layer includes a sulfonated inner surface of the transparent polymer, a silica, a silicon oxycarbide, an O2 plasma treatment of the transparent polymer, or a combination thereof. The device can be a nebulizer or a spray chamber, for example used in an inductively coupled plasma device. A method of making the device is also disclosed.

Abstract: An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the separation unit and further fluid driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit. A control unit controls a pressure of the fluid and/or the further fluid during injecting.

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

Abstract: A device, including a body including a first post and a second post positioned on opposite sides of a recessed area is disclosed. An assembly including the device is disclosed. A method of using the device is also disclosed.