Abstract: A method for analyzing samples utilizing stable label isotope tracing includes receiving mass spectrometry (MS) data generated by an MS system performing untargeted data acquisition on a plurality of samples, performing untargeted feature extraction on the unlabeled compound data to generate a data set of first extracted features, generating a plurality of empirical molecular formulas respectively corresponding to the first extracted features, performing targeted isotopologue extraction on the labeled compound data to generate a data set of second extracted features, wherein the targeted isotopologue extraction is based on the empirical molecular formula and retention time of each first extracted feature, and identifying one or more groups of isotopologues from the second extracted features.

Abstract: The present disclosure provides methods of improving the resolution of analytes by in electrophoretic separations using a gel by incorporating into the gel an effective amount of one or more tri- or tetra-hydroxyl quaternary ammonium compounds, or a mixture of such compounds.

Abstract: A microfluidic apparatus for forming one or more droplets of an aqueous fluid suspended in a non-aqueous fluid is described. The microfluidic apparatus includes a first microfluidic channel configured for flowing an aqueous fluid through the first microfluidic channel and a second microfluidic channel fluidically connected to the first microfluidic channel and adapted to flow a non-aqueous fluid through the second microfluidic channel into the first microfluidic channel. A microfluidic reservoir fluidically connected to the first microfluidic channel and configured to receive a plurality of droplets of the first aqueous fluid. The microfluidic apparatus further includes a first electrode and a second electrode positioned such that application of voltage to the first electrode moves one or more droplets of the aqueous fluid in a first direction and application of voltage to the second electrode moves one or more droplets of the aqueous fluid in a second direction.

Abstract: Reagents and methods for extracting and stabilizing metabolites at room temperature and methods in which metabolites and one or more of proteins, lipids and nucleic acids are extracted from a single sample in a unified workflow.

Abstract: A mixer for mixing a mobile phase in a sample separation device for separating a fluidic sample, wherein the mixer includes a fluid inlet for supplying the mobile phase to be mixed to a mixing volume, a movable body configured for rotating and axially moving in the mixing volume to thereby mix the mobile phase, and a fluid outlet for supplying the mixed mobile phase to a mobile phase consumer.

Abstract: A multi-dimensional liquid chromatography system includes first and second liquid chromatography systems. The first system is configured for providing a first chromatographic separation of a sample fluid comprised in a first mobile phase and to provide a first effluent including at least a portion of the separated sample fluid. The second system is configured for providing a second chromatographic separation of at least a portion of the first effluent comprised in a second mobile phase. A control unit is configured to operate the first liquid chromatography system by maintaining a first flow rate of the first mobile phase substantially constant during the first chromatographic separation, and to operate the second liquid chromatography system during the second chromatographic separation according to a control value different from the second flow rate, so that a variation in the control value can lead to a variation in the second flow rate.

Abstract: The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

Abstract: A valve arrangement includes a valve module and a base module. The valve module includes a stator and a rotor, which is rotatable relative to the stator such that at least one fluid connection is formable between the stator and the rotor. The base module includes at least a part of a force control mechanism for selectively implementing a force-releasing or force-coupling of the rotor and the stator, whereby the valve module and the base module are selectively coupleable to or decoupleable from each other.

Abstract: An electrostatic lens for transporting charged particles in an axial direction includes a first group of first electrodes configured to receive a first DC potential from a DC voltage source, and a second group of second electrodes configured to receive a second DC potential from the DC voltage source different from the first DC potential. The first electrodes are interdigitated with the second electrodes. The first group and/or the second group has a geometric feature that progressively varies along the axial direction. The lens generates an axial potential profile that progressively changes along the axial direction, and thereby reduces geometrical aberrations. The lens may be part of a charged particle processing apparatus such as, for example, a mass spectrometer or an electron microscope.

Abstract: A vacuum pump includes a housing, a rotatable shaft extending in an axial direction within the housing, a first pumping arrangement including a first stator arrangement and a first rotor arrangement, and a second pumping arrangement including a second stator arrangement and a second rotor arrangement. The vacuum pump further includes a spacer arranged between the first pumping arrangement and the second pumping arrangement. The spacer is coupled between the first stator arrangement and the second stator arrangement and is configured to provide a defined elasticity in the axial direction allowing an elastic deformation of the spacer in the axial direction.

Abstract: A coated capillary tube having a tunable resistance in an ion transfer device, including an inlet end in communication with an atmospheric-pressure ion source, an outlet end in communication with a vacuum region of a mass spectrometer, a body elongated along an axis from the inlet end to the outlet end, and an inside surface defining a bore having an inner diameter is disclosed. The coated capillary tube also includes a resistive coating on the inside surface of the capillary tube, in which the resistive coating includes at least one layer comprising oxides or nitrides of a metal and discrete metal particles of a different metal embedded therein.

Abstract: A fluid valve for a sample separation apparatus includes a first valve component and a second valve component, which are adjacent to one another and movable relative to one another. The valve components are configured such that, in at least one switching state, at least one switchable fluid channel is formed between the valve components, and at least one part of a surface of at least one of the first valve component and the second valve component is provided with a coating containing gold and/or platinum.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: A degasser for at least partially degassing a gas-containing liquid, in particular for a sample separation device, includes a circulation path along which the liquid can be circulated between a liquid accommodation volume and one of an inlet to a consumer unit consuming degassed liquid or a conduit leading to the inlet, a drive unit configured for circulating the liquid in the circulation path, and a filter in the circulation path for filtering particles or debris out of the liquid, wherein the liquid is forced through the filter by the drive unit. The drive unit includes a movable body, in particular a movable piston or a movable membrane, configured for at least partially degassing the liquid by generating a negative pressure in the liquid.

Abstract: There is disclosed a method of producing etched non-porous particles. The method includes, in some examples, coating a non-porous particle with a hydrophilic polymer and treating the coated particle with acid or base. Also provided is etched non-porous particles capable of separating a variety of analytes, including biomolecules.