Abstract: Provided herein are methods for assembling DNA fragments employing at least three enzymatic activities: DNA polymerase, flap endonuclease, and DNA ligase. Certain aspects include methods for generating closed circular DNA products, e.g., plasmid vectors, by assembling various DNA fragments having complementary ends that hybridize to one another. The resulting circular products can be introduced into host cells and selected for desired properties. Kits for performing the method are also provided.

Abstract: A dilution apparatus (100) for diluting a fluidic sample in accordance with a specifiable dilution ratio, wherein the dilution apparatus (100) comprises a dilution fluid supply device (102) configured for supplying a dilution fluid at a first quantity per time, a transport fluid supply device (104) configured for supplying a transport fluid at a second quantity per time, a first fluid accommodation unit (106) configured for accommodating a first fluid volume, a second fluid accommodation unit (108) configured for accommodating a second fluid volume, and a control device (110, 112) configured for controlling the flow of the dilution fluid, the transport fluid and the fluidic sample so that in a first operation mode, the fluidic sample, being accommodated in the first fluid accommodation unit (106), is forced to flow to the second fluid accommodation unit (108) while being diluted by being mixed with dilution fluid, and in a second operation mode, the mixture of dilution fluid and fluidic sample, being accommod

Abstract: Ion sources for use in mass spectrometry (MS) systems are described. The ion sources each comprise an ion funnel and an ionization source configured to ionize neutral analyte molecules.

Abstract: A scroll pump has a tip seal between an axial end of the scroll blade of one of stationary and orbiting plate scrolls of the pump and the plate of the other of the stationary plate and orbiting plate scrolls. The scroll pump may have a ballast gas supply system and use the operation of the ballast gas supply system to assess the condition of the tip seal. Alternatively, the scroll pump may have two pressure sensors that sense pressure at two locations spaced along a compression mechanism of the pump to assess the condition of the tip seal.

Abstract: Disclosed is a sample dispatcher configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a separation system configured for separating compounds of the sample fluids. The separation system comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises a plurality of sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof. The sample dispatcher is configured for selectively coupling one of the plurality of sample reservoirs between the mobile phase drive and the separation unit, and further for coupling at least two of the plurality of sample reservoirs in parallel between the mobile phase drive and the separation unit.

Abstract: A sealing element for sealing a fluidic connection between a coupling element and a tubular element and thereby providing a sealed flow path through the tubular element and between the coupling element and the tubular element in a longitudinal direction, the sealing element comprising: a recess extending in the longitudinal direction, the recess configured to receive the tubular element; a transverse wall defining an extent of the recess in the longitudinal direction, the transverse wall having a through hole.

Abstract: Methods and systems for pumping compressible fluids in high pressure applications such as high-pressure liquid chromatography (HPLC) or supercritical fluid chromatography (SFC) applications are disclosed. An improved cooling device for a pump head for use in a supercritical fluid chromatography (SFC) system is described. A system for chilling a pumping system, includes a Peltier cooling element in thermal contact with a pump head, wherein the cooling element chills the pump head and a mobile phase fluid flowstream prior to the mobile phase fluid entering the pump; a fluid-cooled heat exchanger, attached to the Peltier cooling element, which removes heat from the cooling element using a circulating fluid; and a second heat exchanger which cools the circulating fluid.

Abstract: An MFC includes: a proportional valve; a mass flow sensor; a first flow line connecting from an outlet of the proportional valve through the mass flow sensor to an exit line; a second flow line joining the first flow line at a first junction located upstream of the mass flow sensor and at a second junction located downstream of the mass flow sensor; a switching valve placed such that the switching valve can regulate a flow of a gas through the first flow line or the second flow line; and a control device connected to provide a feedback control loop for regulating the proportional valve based on signals measured by the mass flow sensor, wherein the control device includes a program for keeping a rate of a flow exiting the exit line substantially constant when the flow is through the second flow line.

Abstract: A fitting element, in particular for an HPLC application, is configured for providing a fluidic coupling of a tubing to a fluidic device. The fitting element comprises a gripping piece to exert—upon coupling of the tubing to the fluidic device—a grip force (G) between the fitting element and the tubing. The gripping piece comprises a hydraulic element configured to transform an axial force (S) into a hydraulic pressure (P) within the hydraulic element. The hydraulic pressure (P) in the hydraulic element causes the grip force (G).

Abstract: The present disclosure provides nucleoside compounds and oligonucleotides including an unnatural 6-amino-2-pyridone heterocyclic base where the 6-amino and 2-positions are protected. The 2-position of the heterocyclic base can be protected with an acyl-oxy-methyl protecting group. In some embodiments, the protected heterocyclic base has the following structure where AcOM is an acetyl-oxy-methyl group and R is a ribose or deoxyribose sugar: Methods for synthesizing an oligonucleotide are provided in which the subject compounds find use. The method can include protecting an unnatural (e.g., Z) nucleotide with an acetyl-oxy-methyl group; incorporating the protected unnatural nucleotide into a nucleotide sequence on a solid support; and removing the acetyl-oxy-methyl group from the unnatural nucleotide incorporated into the nucleotide sequence. The compounds and methods find use in the synthesis of long oligonucleotides including Z nucleotides.

Abstract: A pump unit comprises a primary piston pump, a secondary piston pump, and a flow path adapted for fluidically connecting in series the primary piston pump and the secondary piston pump. The pump unit's duty cycle comprises a delivery-and-fill phase, in which the primary piston pump supplies a flow of liquid to the secondary piston pump, and during the delivery-and-fill phase, the flow of liquid supplied by the primary piston pump is partly used for filling up the secondary piston pump and partly used for maintaining another flow of liquid dispensed across the secondary piston pump. The flow path comprises a heat exchanger, wherein liquid supplied by the primary piston pump passes through the heat exchanger before being supplied to the secondary piston pump.

Abstract: An ion guide generates a radio frequency (RF) field to radially confine ions to an ion beam along a guide axis as the ions are transmitted through the ion guide. The effective potential of the RF field has potential wells distributed along the guide axis. The RF field is constructed such that the potential wells move in an axial direction toward an exit end of the ion guide.

Abstract: A fluid supply system (150) adapted for metering two or more fluids in controlled proportions and for supplying a resultant mixture, the fluid supply system (150) comprising a plurality of solvent supply lines (104 to 107), each fluidically connected with a fluid source (100 to 103) providing a respective fluid, a pumping unit (110) comprising a reciprocating element (115) adapted for intaking fluid supplied at an inlet of the pumping unit (110) and for supplying the pressurized fluid at an outlet of the pumping unit (110), wherein the pumping unit (110) is adapted for taking in fluids from selected solvent supply lines (104 to 107) and for supplying a pressurized mixture of the fluids at its outlet, a proportioning valve (108) interposed between the solvent supply lines (104 to 107) and the inlet of the pumping unit (110), the proportioning valve (108) adapted for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines (104 to 107) with the inlet of the pumping unit

Abstract: A device for analyzing measurement data having a plurality of data sets, each data set being assigned to a respective one of a plurality of measurements, each data set having multiple features being indicative of different fractions of a fluidic sample, the device comprising a cluster determining unit configured for determining feature clusters by clustering features from different data sets presumably relating to the same fraction, a spread determining unit configured for determining for at least a part of the feature clusters a spread of the features within a respective feature cluster, and a display unit configured for displaying at least the part of the feature clusters together with a graphical indication of the corresponding spread.

Abstract: An adjustable aperture device for an electromagnetic radiation detecting apparatus includes a position adjustment body configured for adjusting a position of a selected aperture hole of multiple selectable aperture holes, where electromagnetic radiation propagates through the selected aperture hole. The adjustable aperture device further includes a guide unit configured for guiding the position adjustment body along a predefined guide direction, and an aperture body defining the aperture holes and including multiple engagement sections, where the adjustment body is engagable in a selectable one of the engagement sections to thereby select the selected aperture hole. The adjustable aperture device further includes a pre-loading element configured for pre-loading the position adjustment body towards the aperture body, and a drive unit configured for driving the aperture body to move so that the position adjustment body is engaged in a respective one of the plurality of engagement sections.

Abstract: A fitting for providing a fluid connection between a capillary and a fluidic conduit of a fluidic component, wherein the fitting comprises a capillary reception configured for receiving the capillary, a force applicator configured for being operable to apply a fixing force for fixing the capillary within the fitting, a force limitation mechanism configured for limiting the fixing force being applicable by the force applicator to the capillary, a force splitter configured for splitting the fixing force into an advance force component for advancing the capillary received in the capillary reception towards the fluidic component and into a clamping force component for clamping the capillary received in the capillary reception within the fitting, and a biasing mechanism particularly arranged between the force applicator and the force splitter and configured for biasing the force splitter against the capillary.

Abstract: The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.

Abstract: A fluid supply system configured for supplying fluids includes a fluid packet supply unit configured for controlling supply of a sequence of fluid packets. The fluid packets include a packet of first fluid and a packet of second fluid, wherein the first fluid and the second fluid are media being prone to a phase separation upon direct interaction between the packet of first fluid and the packet of second fluid. The fluid supply system further includes a phase separation inhibiting unit configured for inhibiting phase separation by inserting an intermediate fluid packet between the packet of first fluid and the packet of second fluid.

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.