Patents Assigned to Agilent Technologies, Inc.
  • FILTER ASSEMBLIES, DEPTH INDICATORS, TORQUE-LIMITING FITTINGS, TORQUE-INDICATING FITTINGS, AND SYSTEMS INCORPORATING THE SAME
    Publication number: 20220196612
    Abstract: In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.
    Type: Application
    Filed: March 9, 2022
    Publication date: June 23, 2022
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Alan D. LOUX, Lucas SERGE, Manuel VAN-VENROOY, Thomas HARRISON, Matthias KAMUF
  • COMPOSITIONS COMPRISING CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED EDITING OF HBB
    Publication number: 20220195427
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Application
    Filed: March 14, 2022
    Publication date: June 23, 2022
    Applicants: The Board of Trustees of the Leland Stanford Junior University, Agilent Technologies, Inc.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED GENE CORRECTION
    Publication number: 20220195426
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Application
    Filed: March 14, 2022
    Publication date: June 23, 2022
    Applicants: The Board of Trustees of the Leland Stanford Junior Univerisity, Agilent Technologies, Inc.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • FILTER ASSEMBLIES, DEPTH INDICATORS, TORQUE-LIMITING FITTINGS, TORQUE-INDICATING FITTINGS, AND SYSTEMS INCORPORATING THE SAME
    Publication number: 20220196613
    Abstract: In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.
    Type: Application
    Filed: March 9, 2022
    Publication date: June 23, 2022
    Applicant: Agilent Technologies, Inc.
    Inventors: Alan D. Loux, Lucas Serge, Manuel Van-Venrooy, Thomas Harrison, Matthias Kamuf
  • Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
    Patent number: 11360072
    Abstract: A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.
    Type: Grant
    Filed: January 3, 2020
    Date of Patent: June 14, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Xiaobo Wang, Yama A. Abassi, Biao Xi, Wen Fu Zhang, Xiao Xu
  • Ion pump shield
    Patent number: 11355327
    Abstract: An ion pump with a housing enclosing an interior, a gas inlet having a through-hole extending into the interior of the ion pump, at least one cathode, at least one anode positioned in proximity to the at least one cathode, a magnet disposed on an opposite side of the at least one cathode from the anode, and a blocking shield disposed between the gas inlet and the at least one cathode. The blocking shield is electrically connected to the at least one anode. An associated method installs the blocking shield by inserting components of the blocking shield assembly through the gas inlet, and assembling (inside the interior of the ion pump) the inserted components to form the blocking shield.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: June 7, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Cristian Maccarrone, Chiara Paolini, Enrica Carbonero, Paolo Manassero
  • System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
    Patent number: 11346797
    Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: May 31, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Xiaobo Wang, Wei Ouyang, Nan Li, Tianxing Wang, Xiaoyu Zhang, Xiao Xu, Yama A. Abassi
  • Branching off fluidic sample with low influence on source flow path
    Patent number: 11346822
    Abstract: A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: May 31, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Thomas Ortmann, Daniel Thielsch
  • Multi frequency LC resonator topologies applicable to mass spectrometer radio-frequency drive systems
    Patent number: 11342169
    Abstract: In one embodiment, a power source for providing high-voltage radio-frequency (RF) energy to an instrument such as a mass spectrometer includes an RF power amplifier having an output, an oscillating RF signal generator configured to provide first and second RF signals respectively oscillating at first and second frequencies to the RF power amplifier, and a step-up circuit for magnifying the RF power amplifier output. The step-up circuit includes an LC resonator network tuned to the first and second frequencies, and an output for providing the magnified voltage to a rod assembly of the mass spectrometer.
    Type: Grant
    Filed: April 8, 2020
    Date of Patent: May 24, 2022
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventor: Michael Schoessow
  • UV-absorbance multichannel capillary electrophoresis system
    Patent number: 11340191
    Abstract: A capillary electrophoresis system includes a capillary reservoir. The capillary reservoir includes a capillary tip flow chamber configured to receive respective capillary tips and to conduct fluid past the capillary tips, and an electrode flow chamber in which an electrode is disposed and configured to conduct fluid past the electrode, the electrode flow chamber being separate from and in fluid communication with the capillary tip flow chamber. An ultraviolet (UV) light absorbance-based multiplexed capillary electrophoresis system includes a first enclosure and a second enclosure. The first enclosure covers a UV light source, and includes a slit. The second enclosure covers the first enclosure, a collimating lens, and a capillary window.
    Type: Grant
    Filed: August 15, 2017
    Date of Patent: May 24, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Michael Stebniski, Bruce R. Boeke, Martin Chris Foster, Scott Stueckradt, Thomas J. Kurt
  • Orthoester compositions for affinity purification of oligonucleotides
    Patent number: 11299483
    Abstract: Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: April 12, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Joel Myerson, Brian Smart
  • Mid-infrared scanning system
    Patent number: 11300773
    Abstract: An apparatus and method for generating a mid-infrared region image of a specimen are disclosed. The apparatus includes a mid-infrared region laser that generates a first light beam, and a stage adapted to carry a specimen to be scanned. An optical assembly focuses the first light beam to a point on the specimen. A first light detector measures a first intensity of light leaving the point on the specimen. A stage actuator assembly causes the specimen to move relative to the point in two dimensions. A controller forms a mid-infrared region image from the first intensity. The image can be based on reflected or transmitted light. The maximum size of the imaged area is determined by a scanning assembly that moves in a first direction relative to the stage, the stage moving in a direction orthogonal to the first direction.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: April 12, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Charles Hoke, Varun Raghunathan
  • Gas Leak Detector Cartridge
    Publication number: 20220099518
    Abstract: A gas leak detector cartridge includes a cartridge housing that is removably installed in a handheld device. The cartridge housing includes a sensor that detects a presence of a contaminant in a sample gas and is connected to a printed circuit board. The cartridge housing also includes a pump positioned in a manifold and introduces a sample gas to the sensor. The sensor is at least partially inserted into the manifold that includes the pump.
    Type: Application
    Filed: September 28, 2020
    Publication date: March 31, 2022
    Applicant: Agilent Technologies, Inc.
    Inventors: Chin-Seong Khor, Jon-Wen Tan, Heng-Dar Ling
  • System and method for detecting multiple-excitation-induced light in a flow channel
    Patent number: 11287379
    Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.
    Type: Grant
    Filed: March 27, 2020
    Date of Patent: March 29, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Nan Li, Jian Wu, Ye Chen, Tianxing Wang, Xiaobo Wang
  • Sample injection with fluidic connection between fluid drive unit and sample accommodation volume
    Patent number: 11275062
    Abstract: An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the separation unit and further fluid driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit. A control unit controls a pressure of the fluid and/or the further fluid during injecting.
    Type: Grant
    Filed: March 27, 2020
    Date of Patent: March 15, 2022
    Assignee: Agilent Technologies, Inc
    Inventors: Daniel Thielsch, Thomas Ortmann, Sam Wouters
  • Microfluidic check valve and related devices and systems
    Patent number: 11255465
    Abstract: A microfluidic check valve includes an inlet bore, an internal chamber, an outlet bore, and a disk freely movable in the chamber between an open position and a closed position. At the open position, the disk permits fluid to flow from the inlet bore, through the chamber, and to the outlet bore. At the closed position, the disk prevents fluid from flowing in the reverse direction from the chamber into the inlet bore. The check valve may be positioned in-line with a fluid conduit, and/or incorporated with various fluidic devices such as, for example, capillary tubes, fittings, and chromatography columns. The check valve is capable of withstanding high fluid pressures, while featuring a small swept volume, such as a nano-scale volume. The check valve may be utilized, for example, to prevent fluid back flow and isolate pressure pulses in fluid flow systems.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: February 22, 2022
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Ares Geovanos, Hongfeng Yin
  • Microfluidic dielectrophoretic droplet extraction
    Patent number: 11253859
    Abstract: A microfluidic apparatus for separating a droplet of an emulsion in a microfluidic environment is described. The microfluidic apparatus includes a flow cell comprising a first microfluidic channel configured for flowing a first fluid through the flow cell and a second microfluidic channel configured for flowing a stream of a second fluid through the flow cell. The microfluidic apparatus further comprises a first electrode positioned at the first microfluidic channel and a second electrode positioned at the second microfluidic channel on an opposite side of the interface with respect to the first electrode. The first electrode, the second electrode, and the first and second microfluidic channels are configured to generate a non-uniform electric field gradient in the microfluidic apparatus.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: February 22, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Curt A. Flory, Dustin Chang
  • Method for finding species peaks in mass spectrometry
    Patent number: 11244818
    Abstract: A method for operating a data processing system to find peaks in a mass spectrum that includes an ordered set of measurements of the abundances of species as a function of the mass/charge ratio of the species is disclosed. The method includes selecting a candidate blob that has a plurality of blob peaks from the mass spectrum. The data processing system selects a candidate blob peak for characterization. The candidate blob peak is approximated by a first species peak using a species peak model having a plurality of parameters by fitting the species peak model to a portion of the blob that has values that are substantially free of contributions from other species peaks that overlap with the first species peak and that are not represented by the species peak model. The first species peak is then subtracted from the candidate blob.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: February 8, 2022
    Assignee: Agilent Technologies, Inc.
    Inventor: Daniel Y. Abramovitch
  • Preparation of tissue sections using fluorescence-based detection
    Patent number: 11243148
    Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
    Type: Grant
    Filed: December 21, 2020
    Date of Patent: February 8, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Kyle Schleifer, Kristin Briana Bernick, Adrienne Mccampbell, Nicholas M. Sampas, Victor Lim
  • Inductively coupled plasma mass spectrometer with mass correction
    Patent number: 11239068
    Abstract: Systems and methods for controlling mass filtering of polyatomic ions in an ion beam passing through an inductively coupled plasma mass spectrometer (ICP-MS). Polyatomic ion mass data representative of the exact mass of a polyatomic ion having a target isotope is determined. A control signal is generated based on the determined polyatomic ion mass data and output to an ICP-MS to filter based on mass the polyatomic ions in the ion beam traveling through the ICP-MS to an ion detector.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: February 1, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Naoki Sugiyama, Amir Liba, Mark Lee Kelinske, Glenn David Woods