Abstract: The invention is a multiplex, pulsed-field capillary electrophoresis instrument with the ability to analyze DNA fragments with sizes greater than 150,000 base pairs. The parallel capillary electrophoresis system allows for the simultaneous analysis of at least 12 samples while applying a pulse or varying electric field for separation. Sequences of pulse-field electric fields are iterated to achieve accurate separation of DNA smears.

Abstract: In gas chromatography (GC), a sample is introduced into a flow of carrier gas and the mixture is driven through a heated GC column to acquire chromatographic data from the sample. During this time, the column is heated from an initial temperature to a final temperature. Subsequently, the column is cooled according to a cooling program. The cooling program may include a first cooling ramp, a subsequent isothermal hold, and a subsequent second cooling ramp. Alternatively, while the column is cooled down the flow of carrier gas through the column may be slowed down or ceased for a period of time, after which the flow of carrier gas through the column may be resumed at the original flow rate in preparation for processing another sample. Controlling column temperature and/or flow in this manner may be effective for reducing column bleed carryover and/or the effects thereof.

Abstract: The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds, such as N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample. Use of activated procaine as a label allows for sensitive detection of compounds labeled with it both by fluorescence and by mass spectrometry.

Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

Abstract: An apparatus for receiving a chemical species is disclosed. The apparatus includes an emission block configured to receive a vapor phase chemical species that is excited by chemical reaction in the emission block to provide an excited species that emits light. The emission block has an interior portion having a surface. A coating is disposed over the surface and the coating reduces adsorption of the excited species onto the surface.

Abstract: A pre-heater assembly for pre-heating a fluid, in particular in a fluid separation apparatus, wherein the pre-heater assembly comprises a capillary having a lumen and being configured for conducting the fluid, and a thermal coupling body contacting at least part of the capillary, having a value of thermal conductivity in a range between 8 W/(m K) and 100 W/(m K) and being arrangable so that heat generated by a heat source is supplied to the capillary via at least part of the thermal coupling body.

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply mean (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

Abstract: A fitting element is configured for coupling tubing to a fluidic device having a receiving cavity configured for receiving the fitting element, where the tubing has an inner contact surface of a biocompatible material, the inner contact surface being configured to contact a fluid to be conducted by the tubing, and the receiving cavity having a receiving contact surface of a bio-compatible material. The fitting element includes a first sealing element of a bio-compatible material configured for sealing to the bio-compatible material of the inner contact surface of the tubing, and a second sealing element configured for sealing against a pressure ambient to a pressure of the fluid in the tubing. Upon coupling of the tubing to the fluidic device, at least a portion of the receiving contact surface, the first sealing element, and the second sealing element enclose an interspace, each surface of the interspace being a bio-compatible material.

Abstract: To determine a corrected flow rate value of a mobile phase flowing in a liquid chromatography (LC) system, a flow rate of the mobile phase at a selected reference position in the LC system is measured to produce one or more flow rate values. A model of the LC system is applied to the flow rate value(s) to determine the corrected flow rate value(s). The model includes a distribution of capacitive elements and resistive elements arranged according to a topology of the LC system. The capacitive and resistive elements are representative of system and solvent properties affecting flow rate while operating the LC system. The system and solvent properties may be properties affecting the flow rate while operating the LC system under dynamic conditions.

Abstract: Novel compounds carrying ligands capable of binding to counter receptors on relevant target cells are disclosed. The compounds possess a number of advantageous features, rendering them very suitable for a wide range of applications, including use as detection systems, detection of relevant target cells as well as a number of other methods. In particular, novel MHC complexes comprising one or more MHC molecules containing one or more Borrelia derived peptides are disclosed. The possibility of presenting to the target cells a plurality of MHC-peptide complexes makes the MHC complexes according to the present invention an extremely powerful tool e.g. in the field of therapy and diagnosis. The invention generally relates to the sample-mounted use of MHC complexes and MHC multimers. Also comprised by the invention is the field of therapy and vaccine, including therapeutic/vaccine methods and therapeutic/vaccine compositions.

Abstract: A high-vacuum pump comprises a plurality of pumping stages, each comprising a plurality of mutually cooperating elements, including at least one rotating rotor element and one stationary stator element. At least one of the elements of at least one of the pumping stages is made of a plastic material reinforced with short fibres, dispersed in chaotic and substantially random manner inside the matrix of plastic material. Use of a plastic material reinforced with short fibres allows making the at least one element by injection molding and allows manufacturing the vacuum pump with considerably reduced production costs if compared to the conventional vacuum pumps.

Abstract: A sample dispatcher is disclosed and is configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a liquid separation system. The liquid separation system is configured for separating compounds of the sample fluids and comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises one or more sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof, and a bypass channel.

Abstract: The present disclosure related to apparatus and methods for removing waste of debris from a microtome blade, a microtome blade holder, or both. The invention also relates to automated systems and methods for cleaning a microtome.

Abstract: A system and method for detecting energetic particles include a detector onto which the particles are impinged. An output signal from the detector, indicative of the energy of the particles, is directed by an AC-coupler to a measurement device to determine particle characteristics such as mass and/or abundance. The detector is selectively couplable to positive or negative bias voltages, and in one embodiment is differentially biased to eliminate ringing due common-mode excitation. The AC-coupler has capacitively-coupled input and output terminals that are embedded in a transmission line structure including capacitances that in some embodiments serve as the sole energy storage component in order to reduce the effects of parasitic inductance found in conventional detection circuits. In some embodiments, a pulse compensation network is provided, to reduce undershoot and ringing due to remote installation of the AC-coupler caused by reflection of low frequency components blocked by the AC-coupler.

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

Abstract: The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.

Abstract: An arrangement for mounting components in a heating chamber for heating a fluid of a fluid separation apparatus, wherein the arrangement comprises a mounting board having at least one mounting recess each configured for accommodating at least one component, and the at least one component each configured to be mountable in and/or on the at least one mounting recess.