Abstract: The present disclosure provides nucleoside compounds and oligonucleotides including an unnatural 6-amino-2-pyridone heterocyclic base where the 6-amino and 2-positions are protected. The 2-position of the heterocyclic base can be protected with an acyl-oxy-methyl protecting group. In some embodiments, the protected heterocyclic base has the following structure where AcOM is an acetyl-oxy-methyl group and R is a ribose or deoxyribose sugar: Methods for synthesizing an oligonucleotide are provided in which the subject compounds find use. The method can include protecting an unnatural (e.g., Z) nucleotide with an acetyl-oxy-methyl group; incorporating the protected unnatural nucleotide into a nucleotide sequence on a solid support; and removing the acetyl-oxy-methyl group from the unnatural nucleotide incorporated into the nucleotide sequence. The compounds and methods find use in the synthesis of long oligonucleotides including Z nucleotides.

Abstract: An ion guide generates a radio frequency (RF) field to radially confine ions to an ion beam along a guide axis as the ions are transmitted through the ion guide. The effective potential of the RF field has potential wells distributed along the guide axis. The RF field is constructed such that the potential wells move in an axial direction toward an exit end of the ion guide.

Abstract: A fluid supply system (150) adapted for metering two or more fluids in controlled proportions and for supplying a resultant mixture, the fluid supply system (150) comprising a plurality of solvent supply lines (104 to 107), each fluidically connected with a fluid source (100 to 103) providing a respective fluid, a pumping unit (110) comprising a reciprocating element (115) adapted for intaking fluid supplied at an inlet of the pumping unit (110) and for supplying the pressurized fluid at an outlet of the pumping unit (110), wherein the pumping unit (110) is adapted for taking in fluids from selected solvent supply lines (104 to 107) and for supplying a pressurized mixture of the fluids at its outlet, a proportioning valve (108) interposed between the solvent supply lines (104 to 107) and the inlet of the pumping unit (110), the proportioning valve (108) adapted for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines (104 to 107) with the inlet of the pumping unit

Abstract: A device for analyzing measurement data having a plurality of data sets, each data set being assigned to a respective one of a plurality of measurements, each data set having multiple features being indicative of different fractions of a fluidic sample, the device comprising a cluster determining unit configured for determining feature clusters by clustering features from different data sets presumably relating to the same fraction, a spread determining unit configured for determining for at least a part of the feature clusters a spread of the features within a respective feature cluster, and a display unit configured for displaying at least the part of the feature clusters together with a graphical indication of the corresponding spread.

Abstract: The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.

Abstract: An adjustable aperture device for an electromagnetic radiation detecting apparatus includes a position adjustment body configured for adjusting a position of a selected aperture hole of multiple selectable aperture holes, where electromagnetic radiation propagates through the selected aperture hole. The adjustable aperture device further includes a guide unit configured for guiding the position adjustment body along a predefined guide direction, and an aperture body defining the aperture holes and including multiple engagement sections, where the adjustment body is engagable in a selectable one of the engagement sections to thereby select the selected aperture hole. The adjustable aperture device further includes a pre-loading element configured for pre-loading the position adjustment body towards the aperture body, and a drive unit configured for driving the aperture body to move so that the position adjustment body is engaged in a respective one of the plurality of engagement sections.

Abstract: A fitting for providing a fluid connection between a capillary and a fluidic conduit of a fluidic component, wherein the fitting comprises a capillary reception configured for receiving the capillary, a force applicator configured for being operable to apply a fixing force for fixing the capillary within the fitting, a force limitation mechanism configured for limiting the fixing force being applicable by the force applicator to the capillary, a force splitter configured for splitting the fixing force into an advance force component for advancing the capillary received in the capillary reception towards the fluidic component and into a clamping force component for clamping the capillary received in the capillary reception within the fitting, and a biasing mechanism particularly arranged between the force applicator and the force splitter and configured for biasing the force splitter against the capillary.

Abstract: A fluid supply system configured for supplying fluids includes a fluid packet supply unit configured for controlling supply of a sequence of fluid packets. The fluid packets include a packet of first fluid and a packet of second fluid, wherein the first fluid and the second fluid are media being prone to a phase separation upon direct interaction between the packet of first fluid and the packet of second fluid. The fluid supply system further includes a phase separation inhibiting unit configured for inhibiting phase separation by inserting an intermediate fluid packet between the packet of first fluid and the packet of second fluid.

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

Abstract: System and method for testing a pump. The testing of the pump includes determining a Mechanical Power Index (MPI) for the pump. The MPI is determined by operating the pump at operating speed for a minimum operating interval. When the pump is stopped under normal operation by the application, the pump speed is continuously monitored. The time when the pump speed decreases to an initial speed close to the operating speed is read as an initial time. When the pump speed decreases to a final pump speed close to the critical speed, the end time is read and the elapsed time is determined as ?t=end time?initial time. The MPI is calculated to be the initial pump speed minus the final pump speed divided by ?t. The MPI can be used during the lifetime of the pump to determine when the pump is deteriorating.

Abstract: A method of sample analysis is provided. In certain embodiments, the method may comprise: contacting a nucleic acid sample with a first primer and a second primer under PCR conditions to produce a double stranded product, wherein the second primer comprises a first label and is 5? blocked; b) contacting the double stranded product with an exonuclease to degrade one strand of the double-stranded product to produce a single stranded product; c) contacting the single stranded product with a third primer under primer extension conditions, wherein the third primer comprises a second label; and d) detecting the first and second labels of the partial duplex. A kit for practicing the method is also provided.

Abstract: The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

Abstract: An optical emission spectrometer system includes a light source and a dichroic beam combiner. The light source emits first light in a first direction and second light in a second direction different from the first direction. The dichroic beam combiner receives the first light via a first light path and the second light via a second light path, reflects a portion the first light into an entrance aperture of a light detection and measurement apparatus, and transmits a portion of the second light into the entrance aperture, enabling analysis and measurement of both first and second light characteristics simultaneously. The portion of the first light reflected into the entrance aperture predominately has wavelengths in a first range of wavelengths and the portion of the second light transmitted into the entrance aperture predominately has wavelengths in a second range of wavelengths, different from the first range of wavelengths.

Abstract: A single-piece ferrule for a fitting for coupling a capillary to another component of a fluidic device, wherein the ferrule comprises a ferrule body, wherein the ferrule body has a lumen configured for receiving at least a part of the capillary, wherein the ferrule body has a tapering front part configured for forming a sealed connection with a housing of the fitting, wherein a back side of the tapering front part has an annular undercut.

Abstract: A micromachined flow cell includes a substrate and a freestanding tube, delimiting a fluidic conduit therein and being integrally formed from material of the substrate.

Abstract: A sample injector for injecting a fluid into a fluidic path, wherein the sample injector comprises a robot arm configured for moving an injection needle, when being connected to the robot arm, between a fluid container containing the fluid and a seat in fluid communication with the fluidic path, the needle configured for aspirating the fluid from the fluid container, when the needle has been moved to the fluid container, and for injecting aspirated fluid into the fluidic path, when the needle is accommodated in the seat, and the seat configured for accommodating the needle and providing fluid communication with the fluidic path, wherein the robot arm is configured for selectively disconnecting the needle from the robot arm when the needle is accommodated in the seat, and wherein the robot arm is configured for performing a further task while the needle is disconnected from the robot arm.

Abstract: A method and apparatus for obtaining reference samples during the generation of a mid-infrared (MW) image without requiring that the sample being imaged be removed is disclosed. A tunable MIR laser generates a light beam that is focused onto a specimen on a specimen stage that moves the specimen in a first direction. An optical assembly includes a scanning assembly having a focusing lens and a mirror that moves in a second direction, different from the first direction, relative to the stage such that the focusing lens maintains a fixed distance between the focusing lens and the specimen stage. A light detector measures an intensity of light leaving the point on the specimen. A controller forms an image from the measured intensity. A reference stage is positioned such that the mirror moves over the reference stage in response to a command so that the controller can also make a reference measurement.

Abstract: An injection needle cartridge for a sample injector for injecting a sample fluid into a mobile phase in a fluidic path of a fluid separation system between a mobile phase drive and a separation unit, the injection needle cartridge comprising an injection needle configured for aspirating the sample fluid from a fluid container when the injection needle has been moved to the fluid container, and for injecting aspirated sample fluid into the fluidic path when the injection needle is sealingly accommodated in a needle seat, and a sealing force generator configured for applying a sealing force to the injection needle for sealingly accommodating the injection needle in the needle seat, wherein the injection needle cartridge is configured for being substitutably mountable on a handling robot of the sample injector for handling the injection needle cartridge between the fluid container and the needle seat.

Abstract: The invention provides novel oligonucleotide probes that have dendrimeric dyes useful for detecting and analyzing biological samples, and compositions and methods thereof. The dendrimeric dye-containing oligonucleotide probes are useful for high sensitivity fluorescence in situ hybridization (FISH) of nucleic acids such as DNA and RNA.

Abstract: A light field representation of a sample is synthesized or simulated based on bright field image data and phase image data acquired by a microscope such as a quantitative phase microscope. The light field representation may be utilized to render three-dimensional representations of the sample.