Abstract: A fluid transfer device transfers a fluid from a first fluid channel with a first cross-sectional area into a second fluid channel with a second cross-sectional area, larger than the first cross-sectional area. The fluid transfer device includes a fluid inlet interface at which the fluid is transferable from the first fluid channel into the fluid transfer device; an inlet branch configured to split the fluid from the first fluid channel into multiple inlet branch channels; multiple outlet branches, each of which is configured to split the fluid from the inlet branch channels into respective outlet branch channels; and a fluid outlet interface configured to transfer the fluid in the outlet branch channels into the second fluid channel. The inlet and output branches and branch channels are disposed such that the fluid exits from the fluid outlet interface, distributed in a two-dimensional manner across the second cross-sectional area.

Abstract: A scanner and an attenuated total reflection (ATR) objective for use in such scanners are disclosed. The ATR objective includes first and second optical elements and an input port. The input port receives an input collimated light beam that is focused to a point on a planar face of the first optical element by the second optical element such that substantially all of that portion is reflected by the planar face and no portion of the input beam strikes the planar face at an angle less than the critical angle. The second optical element also generates an output collimated light beam from light reflected from the planar thce that is characterized by a central ray that is coincident with the central ray of the input collimated light beam. A light beam converter receives the first collimated light beam and generates the input collimated light beam therefrom.

Abstract: Provided herein is a reaction mixture comprising Cas9 and a non-ionic surfactant, e.g., a polyoxyethylene surfactant. In certain embodiments, the reaction mixture may comprise a Cas9 protein, a guide RNA, a salt, a buffering agent, a nucleic acid target and a non-ionic surfactant. Kits are also provided. In certain embodiments, a kit may comprise: a Cas9 protein; and a concentrated reaction buffer comprising salt, a buffering agent and a non-ionic surfactant.

Abstract: The present invention relates to an isothermal method for detecting in a sample a target nucleic acid strand.

Abstract: Disclosed is a pressure determining unit configured for determining a pressure of a fluid. The pressure determining unit comprises a body structure and a deformation detector. The body structure has a fluidic path configured for conducting the fluid, wherein the body structure has a first surface in a first dimension and in a second dimension, and a thickness in a third dimension. The deformation detector is configured for responding to an elongation into the second dimension of the first surface of the body structure by generating a signal indicative of a value of the pressure of the fluid in the body structure. The fluidic path of the body structure comprises one or more first channel segments, each first channel segment having a height into the third dimension being at least twice of its width into the second dimension.

Abstract: A device for processing measurement data assigned to a measurement on a fluidic sample to be separated, the measurement data having multiple features being indicative of different fractions of the fluidic sample, the device includes a feature position analysis unit configured for analyzing positions (p1, p2, d1, d2) of at least a part of the features relative to one another, and a display adjustment unit configured for adjusting, based on a result of the analyzing, a mode of displaying at least a part of the features along at least one display axis of a display diagram so that at least a part of adjacent features is positioned equidistantly along the at least one display axis or so that at least a part of adjacent features is positioned to have a distance (d) from one another which is larger than or equal to a predefined threshold value (dth).

Abstract: An apparatus that facilitates the manual sorting of objects is disclosed. The apparatus includes a display surface having a surface that can be selectively illuminated and that is adapted for receiving the objects. An identification reader reads identification information stored on the objects. A controller causes an area on the display surface corresponding to one of the objects to be illuminated based on the identification information and indicates a location to which the illuminated object is to be moved. In one aspect of the invention, the display surface includes an area adapted for positioning a receiver for the objects. The display surface provides an indication of a position in the receiver at which the one of the objects is to be placed.

Abstract: This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.

Abstract: A dilution apparatus (100) for diluting a fluidic sample in accordance with a specifiable dilution ratio, wherein the dilution apparatus (100) comprises a dilution fluid supply device (102) configured for supplying a dilution fluid at a first quantity per time, a transport fluid supply device (104) configured for supplying a transport fluid at a second quantity per time, a first fluid accommodation unit (106) configured for accommodating a first fluid volume, a second fluid accommodation unit (108) configured for accommodating a second fluid volume, and a control device (110, 112) configured for controlling the flow of the dilution fluid, the transport fluid and the fluidic sample so that in a first operation mode, the fluidic sample, being accommodated in the first fluid accommodation unit (106), is forced to flow to the second fluid accommodation unit (108) while being diluted by being mixed with dilution fluid, and in a second operation mode, the mixture of dilution fluid and fluidic sample, being accommod

Abstract: Provided herein are methods for assembling DNA fragments employing at least three enzymatic activities: DNA polymerase, flap endonuclease, and DNA ligase. Certain aspects include methods for generating closed circular DNA products, e.g., plasmid vectors, by assembling various DNA fragments having complementary ends that hybridize to one another. The resulting circular products can be introduced into host cells and selected for desired properties. Kits for performing the method are also provided.

Abstract: Ion sources for use in mass spectrometry (MS) systems are described. The ion sources each comprise an ion funnel and an ionization source configured to ionize neutral analyte molecules.

Abstract: Disclosed is a sample dispatcher configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a separation system configured for separating compounds of the sample fluids. The separation system comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises a plurality of sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof. The sample dispatcher is configured for selectively coupling one of the plurality of sample reservoirs between the mobile phase drive and the separation unit, and further for coupling at least two of the plurality of sample reservoirs in parallel between the mobile phase drive and the separation unit.

Abstract: A scroll pump has a tip seal between an axial end of the scroll blade of one of stationary and orbiting plate scrolls of the pump and the plate of the other of the stationary plate and orbiting plate scrolls. The scroll pump may have a ballast gas supply system and use the operation of the ballast gas supply system to assess the condition of the tip seal. Alternatively, the scroll pump may have two pressure sensors that sense pressure at two locations spaced along a compression mechanism of the pump to assess the condition of the tip seal.

Abstract: A sealing element for sealing a fluidic connection between a coupling element and a tubular element and thereby providing a sealed flow path through the tubular element and between the coupling element and the tubular element in a longitudinal direction, the sealing element comprising: a recess extending in the longitudinal direction, the recess configured to receive the tubular element; a transverse wall defining an extent of the recess in the longitudinal direction, the transverse wall having a through hole.

Abstract: Methods and systems for pumping compressible fluids in high pressure applications such as high-pressure liquid chromatography (HPLC) or supercritical fluid chromatography (SFC) applications are disclosed. An improved cooling device for a pump head for use in a supercritical fluid chromatography (SFC) system is described. A system for chilling a pumping system, includes a Peltier cooling element in thermal contact with a pump head, wherein the cooling element chills the pump head and a mobile phase fluid flowstream prior to the mobile phase fluid entering the pump; a fluid-cooled heat exchanger, attached to the Peltier cooling element, which removes heat from the cooling element using a circulating fluid; and a second heat exchanger which cools the circulating fluid.

Abstract: An MFC includes: a proportional valve; a mass flow sensor; a first flow line connecting from an outlet of the proportional valve through the mass flow sensor to an exit line; a second flow line joining the first flow line at a first junction located upstream of the mass flow sensor and at a second junction located downstream of the mass flow sensor; a switching valve placed such that the switching valve can regulate a flow of a gas through the first flow line or the second flow line; and a control device connected to provide a feedback control loop for regulating the proportional valve based on signals measured by the mass flow sensor, wherein the control device includes a program for keeping a rate of a flow exiting the exit line substantially constant when the flow is through the second flow line.

Abstract: A fitting element, in particular for an HPLC application, is configured for providing a fluidic coupling of a tubing to a fluidic device. The fitting element comprises a gripping piece to exert—upon coupling of the tubing to the fluidic device—a grip force (G) between the fitting element and the tubing. The gripping piece comprises a hydraulic element configured to transform an axial force (S) into a hydraulic pressure (P) within the hydraulic element. The hydraulic pressure (P) in the hydraulic element causes the grip force (G).

Abstract: A pump unit comprises a primary piston pump, a secondary piston pump, and a flow path adapted for fluidically connecting in series the primary piston pump and the secondary piston pump. The pump unit's duty cycle comprises a delivery-and-fill phase, in which the primary piston pump supplies a flow of liquid to the secondary piston pump, and during the delivery-and-fill phase, the flow of liquid supplied by the primary piston pump is partly used for filling up the secondary piston pump and partly used for maintaining another flow of liquid dispensed across the secondary piston pump. The flow path comprises a heat exchanger, wherein liquid supplied by the primary piston pump passes through the heat exchanger before being supplied to the secondary piston pump.