Abstract: In a high performance liquid chromatography system, wherein a mobile phase is driven through a stationary phase for separating compounds of a sample fluid comprised in the mobile phase, a flow rate of the mobile phase is controlled in dependence on a variation in a control value in the system.

Abstract: Prefilled liquid cartridge for fluidically connecting to a sample separation device for separating of components of a fluidic sample by using liquid of the liquid cartridge, wherein the liquid cartridge comprises a liquid container which is prefilled with liquid and a liquid removal access provided at the liquid container, adapted to be fluidically coupled with at least one liquid conduit of the sample separation device by only inserting the liquid cartridge in a corresponding liquid cartridge accommodation of the sample separation device.

Abstract: The main sources of heat in a scroll pump are cooled efficiently by a single cooling fan so that noise and, in particular, fan-generated noise, can be kept low. The scroll pump includes a pump head assembly, a pump motor, a cooling fan that produces a cooling air flow in the pump, a cowling in which the pump head assembly, pump motor and cooling fan are housed and juxtaposed with one another in an axial direction of the pump, and a shroud disposed within the cowling and extending around the pump motor to define a tunnel with the motor and through which the air flow of the cooling fan is directed.

Abstract: The invention provides novel ion-exchange media and related methods for their preparation and use. Ion-exchange stationary phases according to the invention are suitable for chromatographic separation of a variety of biomolecules. Distinguishing characteristics of ion-exchange media according to this invention includes, for example, their ability to separate variants of monoclonal antibodies via cation-exchange liquid chromatography using porous substrates with particle sizes <5 ?m. The ion-exchange stationary media include a hydrolytically stable layer, which inhibits surface degradation of the particles in 100% aqueous media. Another unique feature is low molecular weight building blocks used to functionalize the particles with ion-exchange groups.

Abstract: A vacuum scroll pump has a frame, a stationary plate scroll fixed to the frame, an orbiting plate scroll, an eccentric drive mechanism for driving the orbiting plate scroll, and counterbalancing features by which axial loads produced on the eccentric drive mechanism are offset. Scroll blades of the stationary and orbiting plate scrolls are nested to define pockets which constitute a compression stage between opposing front sides of plates of the stationary and orbiting plate scrolls. The counterbalancing features include an axial counterbalancing chamber defined at a back side of the plate of the orbiting plate scroll, i.e., opposite the side at which the compression stage is provided, and a mechanism by which an intermediate one of the pockets can be placed in communication with the counterbalancing chamber through the plate of the orbiting plate scroll.

Abstract: A scanner and method for using the same are disclosed. The scanner includes a stage, a MIR light source, an imaging system, and a controller. The stage is adapted to hold a specimen to be imaged and to move the specimen in a first direction and in a second direction that is orthogonal to the lateral direction. The imaging system forms an image plane of the specimen when the stage is positioned at a second direction distance, z, from a known point in the imaging system. The imaging system forms a plurality of different image planes of the specimen at the illumination wavelength. Each of the plurality of image planes is characterized by a different value of z, the controller determining a value of z for each of a plurality of points on the specimen at which the point on the specimen is in focus.

Abstract: An imaging scanner and a method for using the same are disclosed. The scanner includes a variable attenuator adapted to receive a light beam generated by a MIR laser and that generates an attenuated light beam therefrom characterized by an attenuation level. The scanner includes an optical assembly that focuses the attenuated light beam to a point on a specimen. A light detector measures an intensity of light leaving the point on the specimen, the light detector being characterized by a detector dynamic range. A controller forms a plurality of MIR images from the intensity as a function of position on the specimen, each of the plurality of MIR images being formed with a different level of attenuation of the light beam. The controller combines the plurality of MIR images to generate a combined MIR image having a dynamic range greater than the detector dynamic range.

Abstract: A scanner and an attenuated total reflection (ATR) objective for use in such scanners are disclosed The ATR objective includes first and second optical elements and an input port. The input port receives an input collimated light beam that is focused to a point on a planar face of the first optical element by the second optical element such that substantially all of that portion is reflected by the planar face and no portion of the input beam strikes the planar face at an angle greater than the critical angle. The second optical element also generates an output collimated light beam from light reflected from the planar face that is characterized by a central ray that is coincident with the central ray of the input collimated light beam. A light beam converter receives the first collimated light beam and generates the input collimated light beam therefrom.

Abstract: A mass spectrometry (MS) system may be cleaned by generating plasma and contacting an internal surface of the system to be cleaned with the plasma. The system may be switched between operating in an analytical mode and in a cleaning mode. In the analytical mode a sample is analyzed, and plasma may or may not be actively generated. In the cleaning mode the plasma is actively generated, and the sample may or may not be analyzed.

Abstract: An AC coupler for transmitting high-frequency components of a wideband signal includes a signal conductor and a shielding structure arranged as a transmission line. The signal conductor includes a conductive element and a capacitor configured to block direct current (DC) components of the wideband signal while transmitting high-frequency alternating current (AC) components of the wideband signal. The shielding structure is configured for conducting at least the AC components of the wideband signal while confining electric fields and currents in the shielding structure substantially to a region proximate to the signal conductor. The shielding structure has a width substantially greater than a width of the signal conductor. The difference between the shielding structure width and the signal conductor width may be substantially greater than an offset distance between the signal conductor and the shielding structure.

Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

Abstract: The present invention provides a simple and rapid method for preparing purified transposase complexes that are highly suited for fragmenting DNA. The method includes forming transposase complexes with oligonucleotide adapters in cell lysate, then purifying the complexes from the other substance in the cell lysate. Purification is accomplished using a specific binding pair, in which one member of the pair is bound to an oligonucleotide adapter of the complex and the other member of the pair is bound to a solid substrate. The bound complexes can be immediately used in DNA fragmentation reactions to produce solid substrate-bound DNA fragments, which can be used for any number of purposes, including as templates for amplification and sequencing.

Abstract: A method comprising: synthesizing a set of overlapping oligonucleotides that comprises probe sequences that hybridize to unique sequences in a chromosome, assembling the overlapping oligonucleotides in a way that produces one or more double stranded polynucleotides that each comprises multiple probe sequences, labeling the one or more double stranded polynucleotides to produce one or more labeled probes, and hybridizing the labeled probes to an intact chromosome, in situ, is provided.

Abstract: Multiplexed spectrometry, such as multiplexed ion mobility spectrometry (IMS), time-of-flight mass spectrometry (TOFMS), or hybrid IM-TOFMS, is carried out on a sample, and the resulting measurement data are deconvoluted. Noise may be removed from the measurement data prior to deconvolution. Alternatively or additionally, noise may be removed from the deconvoluted data.

Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.

Abstract: An apparatus that includes a gain chip assembly, an external cavity, and a controller is disclosed. The gain chip assembly includes first and second gain chips that are coupled optically such that light travels serially between the first gain chip and the second gain chip, each gain chip is electrically biased. The electrical bias of the first gain chip is independent of the electrical bias of the second gain chip. The external cavity has a tunable wavelength selective filter that is changed in response to a control signal. Light in the external cavity passes through the gain chip assembly. The controller determines the tunable wavelength selective filter, and the electrical bias of each of the gain chips so as to cause the apparatus to lase at a wavelength specified by a control signal to the controller.

Abstract: A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3? end.

Abstract: A method and apparatus for measuring diffuse and specularly reflected light from a sample to provide a reflection spectrum as a function of wavelength and as a function of position on a sample is disclosed. The apparatus includes a MIR light source that generates an illumination beam of linearly polarized light. An illumination system illuminates a location on a specimen with part of the illumination beam. A linear polarization filter characterized by a polarization axis that defines a direction of polarization of linearly polarized light that is reflected by the linear polarization filter, a first detector that measures an intensity of light leaving the linear polarization filter and a light collection system collects light reflected from the location on the specimen and directs that light to the linear polarization filter. A controller measures an output from the first detector for each of a plurality of different polarization axis positions.

Abstract: A sample droplet generator transforms a segmented array of sample material into a continuous stream of droplets containing analytes. The droplets may serve as a sample source for a wide range of detectors and analytical instruments. As one example, the droplets may be introduced into an ion source of a spectrometer that measures ions produced from the droplets or photons emitted from the droplets.

Abstract: A method and apparatus for obtaining reference samples during the generation of a mid-infrared (MW) image without requiring that the sample being imaged be removed is disclosed. A tunable MIR laser generates a light beam that is focused onto a specimen on a specimen stage that moves the specimen in a first direction. An optical assembly includes a scanning assembly having a focusing lens and a mirror that moves in a second direction, different from the first direction, relative to the stage such that the focusing lens maintains a fixed distance between the focusing lens and the specimen stage. A light detector measures an intensity of light leaving the point on the specimen. A controller forms an image from the measured intensity. A reference stage is positioned such that the mirror moves over the reference stage in response to a command so that the controller can also make a reference measurement.