Patents Assigned to Agilent Technologies
  • Phosphorous protecting groups and methods of preparation and use thereof
    Patent number: 11584773
    Abstract: Aspects of the present disclosure include compositions that make use of phosphorus and/or nucleobase protecting groups which find use in the synthesis of long polynucleotides. Phosphorus protecting groups are provided that help increase the stepwise coupling yield and/or phosphorous protecting groups that can be removed during the oxidation step. Amidine nucleobase protecting groups are provided that find use in the subject compositions and methods which provides for e.g., increased resistance to depurination during polynucleotide synthesis. In some instances, the methods and compositions disclosed herein utilize a combination of the phosphorus and amidine nucleobase protecting groups in the synthesis of polynucleotides having a sequence of 200 or more monomeric units in length. Also provided are methods for synthesizing a polynucleotide (e.g., a DNA) using one or more compounds disclosed herein.
    Type: Grant
    Filed: July 19, 2021
    Date of Patent: February 21, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Luca Monfregola, Marvin Caruthers, Mithun Roy
  • METHODS OF MAKING MEDIA OF SUBSTANTIALLY MONODISPERSE POPULATIONS OF POLYARYLKETONE OR POLYARYLTHIOETHERKETONE PARTICLES, AND CONTAINERS COMPRISING THEM
    Publication number: 20230047460
    Abstract: The invention provides separation media of substantially monodisperse populations of substantially spherical particles of polyarylketone polymers or of thio-analogues of such polymers, of selected sizes, and further provides containers, such as chromatographic columns and cartridges, containing substantially monodisperse populations of such particles.
    Type: Application
    Filed: July 29, 2022
    Publication date: February 16, 2023
    Applicant: Agilent Technologies, Inc.
    Inventors: John William DAVIES, Thomas Jacob COBB, Elizabeth A. BEVAN, Andrea Angelo P. TRIPODI, Andrew F. COFFEY, Keeley Jane MAPP
  • METHODS OF MAKING MONODISPERSE POPULATIONS OF POLYARYLKETONE OR POLYARYLTHIOETHERKETONE PARTICLES
    Publication number: 20230051959
    Abstract: The invention provides methods for making substantially monodisperse populations of substantially spherical particles of polyarylketone polymers or of thio-analogues of such polymers, of selected sizes. Populations of such particles can be used, for example, to form porous devices with greater control of porosity than previously available. In some embodiments, the porous devices are frits, filters, membranes or monoliths.
    Type: Application
    Filed: July 29, 2022
    Publication date: February 16, 2023
    Applicant: Agilent Technologies, Inc.
    Inventors: John William DAVIES, Thomas Jacob COBB
  • METHODS OF MAKING POROUS DEVICES FROM MONODISPERSE POPULATIONS OF POLYARYLKETONE OR POLYARYLTHIOETHERKETONE PARTICLES
    Publication number: 20230052045
    Abstract: The invention provides methods for making porous devices from substantially monodisperse populations of substantially spherical particles of polyarylketone polymers or of thio-analogues of such polymers, of selected sizes. The porous devices allow greater control of porosity than previously available porous devices. In some embodiments, the porous devices are frits, filters, membranes or monoliths.
    Type: Application
    Filed: July 29, 2022
    Publication date: February 16, 2023
    Applicant: Agilent Technologies, Inc.
    Inventors: John William DAVIES, Thomas Jacob Cobb, Ares Geovanos, Thor Miller Wilbanks, Qing BAI
  • Polychromator systems and methods
    Patent number: 11579459
    Abstract: A polychromator system comprising: an optical element defining an aperture; a collimation mirror for receiving light via the aperture and reflecting substantially collimated light; at least a first dispersive optical component and a second dispersive optical component, each configured to disperse the substantially collimated light received from the collimation mirror by different amounts for different wavelengths and to provide cross-dispersed light having different wavelengths of light spaced along a first and second axis; and a focus mirror positioned to focus the cross-dispersed light onto a 2-D array detector to provide a plurality of aperture images of the aperture at a respective plurality of regions of the detector, each of the plurality of aperture images associated with a respective wavelength of the cross-dispersed light.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: February 14, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Sean M. Kent, Lindsay Buck
  • Modified multiplex and multistep amplification reactions and reagents therefor
    Patent number: 11578357
    Abstract: The invention relates to reagents and methods for improving the efficiency of multiplex nucleic acid amplification, in particular where overlapping amplicons are to be generated. The invention also relates to reagents and methods for improving the efficiency of multistep nucleic acid amplification, in particular the performance of two separate amplification reactions designed to occur in sequence in the same reaction mixture or vessel. The invention further relates to reagents and methods for improving multistep nucleic acid amplification reactions by controlling the output of the first amplification reaction. In particular, primers are provided that minimise the formation of aberrant amplification products. Such primers are particularly useful where first and second amplification reactions take place in a single reaction mixture or vessel.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: February 14, 2023
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Jurgen Peter Lode Del Favero, Lien Heyrman, Bart Tegenbos, Dirk Goossens, Joachim De Schrijver
  • Injector with fluid supply and mobile phase discharge
    Patent number: 11567040
    Abstract: Injector (40) for injecting a fluidic sample into a mobile phase in a sample separation apparatus (10), the injector (40) comprising a main flow path (100) between a fluid drive (20) and a sample separation device (30), wherein the fluid drive (20) is adapted to drive the mobile phase and the sample separation device (30) is adapted to separate the fluidic sample that is injected into the mobile phase, a discharge device (104) for discharging an, in particular predetermined, amount of the mobile phase from the main flow path (100), and a supply device (102) for supplying an, in particular predetermined, amount of the fluidic sample and/or of a solvent into the main flow path (100), wherein the discharged amount and the supplied amount compensate each other at least partially.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: January 31, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Thomas Ortmann, Konstantin Shoykhet
  • MULTI-DEVICE REMOVAL AND INSTALLATION TOOL
    Publication number: 20230017417
    Abstract: In some examples, a multi-device removal and installation tool may include a device removal tool including a first side having a first receiver with a first dimension that removably receives a first device, and a second side having a second receiver with a second dimension that removably receives a second device. The first dimension may be different from the second dimension. A torque limit tool may apply, in a first rotation direction of the torque limit tool and the device removal tool, a tightening torque to the first device. Further, the torque limit tool may apply, in a second rotation direction of the torque limit tool and the device removal tool, a loosening torque to the first device. The second rotation direction may be generally opposite to the first rotation direction.
    Type: Application
    Filed: September 28, 2022
    Publication date: January 19, 2023
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventor: Alan D. LOUX
  • TIP FOR INTERFACE CONES
    Publication number: 20230013904
    Abstract: A tip including an alloy of platinum and an alloying element chosen from gold, palladium, ruthenium, osmium, iron, cobalt, nickel, copper, zinc, silver, chromium, manganese, titanium, niobium, scandium, vanadium, yttrium, zirconium, molybdenum, tantalum, tungsten, technetium, cadmium, hafnium, rhenium, less than 5 wt. % of iridium, less than 5 wt. % of rhodium, greater than 20 wt. % iridium, greater than 20 wt. % rhodium, and a combination thereof, relative to the total weight of the alloy is disclosed. An interface cone can include a base and the tip.
    Type: Application
    Filed: July 19, 2021
    Publication date: January 19, 2023
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: James DELLIS, Lawrence MCCANNEY, Gareth PEARSON
  • Precision high voltage power supply with dual feedback loop
    Patent number: 11557974
    Abstract: A high-voltage (HV) power supply outputs an output voltage based on a control signal produced by a dual analog/digital feedback loop. The control signal is determined at least in part by an error amplifier that receives a measurement signal, proportionally attenuated from the output voltage, and a digital-to-analog converter (DAC) output signal. An analog-to-digital converter (ADC) also receives the measurement signal and transmits it in digitized form to a digital processor. The digital processor calculates a digital DAC data signal based on the measurement signal, and on a digital set-point input signal corresponding to a set-point voltage value of the output voltage desired to be outputted from the high-voltage source. A DAC receives the DAC data signal and converts it to the DAC output signal transmitted to the error amplifier.
    Type: Grant
    Filed: April 27, 2021
    Date of Patent: January 17, 2023
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventor: David Deford
  • NSTRUMENT COMPONENT ORGANIZER
    Publication number: 20230011549
    Abstract: In some examples, an organizer may include a plurality of inserts. At least one insert of the plurality of inserts may include at least a front panel and an opposite back panel that form the at least one insert, and at least one instrument component disposed hi the at least one insert. The organizer may further include a binding mechanism that attaches the plurality of inserts.
    Type: Application
    Filed: July 9, 2021
    Publication date: January 12, 2023
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Shaouhan HU, Daniela Loraing, Olga Schikurski, Sascha Lege, Stephan Buckenmaier
  • Molecular fabrication
    Patent number: 11549106
    Abstract: Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: January 10, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Jeffrey Carl Braman, Peter James Sheffield, Gavin Fischer
  • Orthoester compositions for affinity purification of oligonucleotides
    Patent number: 11548876
    Abstract: Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: January 10, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Joel Myerson, Brian Smart
  • Methods to improve reduction or labeling of carbohydrates
    Patent number: 11543414
    Abstract: The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: January 3, 2023
    Assignee: Agilent Technologies, Inc.
    Inventor: Andres Guerrero Navarro
  • METHODS, COMPOSITIONS, AND KITS FOR HIGH-THROUGHPUT RELEASE, LABELING, AND ANALYSIS OF O-GLYCANS PRESENT ON A GLYCOCONJUGATE
    Publication number: 20220412985
    Abstract: The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.
    Type: Application
    Filed: December 10, 2020
    Publication date: December 29, 2022
    Applicant: Agilent Technologies. lnc.
    Inventor: Ace Gita GALERMO
  • Use of transposase and Y adapters to fragment and tag DNA
    Patent number: 11535889
    Abstract: Described herein is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A method, as well as a kit for practicing the method, are also provided.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: December 27, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Robert A. Ach, Nicholas M. Sampas, Brian Jon Peter
  • Chemically modified guide RNAS for CRISPR/Cas-mediated gene regulation
    Patent number: 11535846
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Grant
    Filed: March 14, 2022
    Date of Patent: December 27, 2022
    Assignees: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, AGILENT TECHNOLOGIES, INC.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • Deparaffinization of tissue utilizing electric field
    Patent number: 11525759
    Abstract: Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: December 13, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Adrienne Mccampbell, Victor Lim
  • Sample injector with metering device balancing pressure differences in an intermediate valve state
    Patent number: 11519884
    Abstract: A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve to transfer the sample loop between a low pressure state and a high pressure state via an intermediate state and for controlling the metering device during the intermediate state to at least partially equilibrate a pressure difference in the sample loop between the low pressure state and the high pressure state.
    Type: Grant
    Filed: August 13, 2020
    Date of Patent: December 6, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Bernd Glatz, Wolfgang Kretz
  • Touchless package
    Patent number: D977341
    Type: Grant
    Filed: February 26, 2021
    Date of Patent: February 7, 2023
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Olga Schikurski, Lucas Serge, Qi Siegmundt-Pan, Michael Wherry, Thorsten Dressler, Daniela Loraing, Thomas Dumoulin