Abstract: A column including particles having an average particle size ranging from about 1 ?m to about 5 ?m; the particles have an average pore size ranging from about 450 ? to about 3000 ?; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.

Abstract: A capillary array assembly is fabricated by inserting capillaries into respective grooves of a substrate and fixing the capillaries to the substrate. The capillaries are fixed by applying heat and force to the substrate, with a defined displacement of substrate material and for a defined period of time, thereby spreading heated portions of the substrate over the capillaries. After applying the heat and force, capillaries are at least partially embedded by the substrate and may be at least partially exposed to a top outside surface of the substrate by respective slots. The capillary array assembly may be utilized, for example, in a sample analysis instrument such as a capillary electrophoresis (CE) instrument.

Abstract: The invention provides methods for making porous devices from substantially monodisperse populations of substantially spherical particles of polyarylketone polymers or of thio-analogues of such polymers, of selected sizes. The porous devices allow greater control of porosity than previously available porous devices. In some embodiments, the porous devices are frits, filters, membranes or monoliths.

Abstract: A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.

Abstract: Provided herein is a method for identifying a sequence variant in an enriched sample. In certain embodiments, this method may comprise: (a) obtaining: (i) a plurality of sequence reads from a sample that has been enriched for a genomic region and (ii) a reference sequence for the genomic region; (b) assembling the sequence reads to obtain a plurality of discrete sequence assemblies that correspond to potential variants; (c) determining which of the potential variants are true and which are artifacts by examining the sequence reads that make up each of the discrete sequence assemblies; (d) optionally determining whether each of the true potential variants contains a mutation that is known to be associated with the reference sequence; and (e) outputting a report indicating whether the sample comprises a sequence variant.

Abstract: Flame-based detectors having an ignitor are protected from corrosion by flowing a protective gas over the ignitor. The protective gas layer protects the ignitor from corrosive components in exhaust gas.

Abstract: A flame-based detector (102) comprises a housing (104), a burner (106), a fuel flow path (108), an air flow path (110), a collector (114), an ignitor (116), an ignition promoter port (122), and an exhaust vent (128). The ignition promoter port (122) introduces a gas close to the ignitor (116), thereby facilitating combustion.

Abstract: The present disclosure is directed to a single-end sequencing method for improved detection of genomic rearrangements such as deletions, insertions, inversions, and translocations that are present in a polynucleotide. A first priming event allows for sequencing of a target sequence, and a second priming event on an adapter allows for identification of the sequences amplified and tagged by selective amplification. The combination of priming events in the same direction facilitates read alignment and the identification of any genomic rearrangements.

Abstract: Methods and systems for synthesizing an oligonucleotide in a reaction chamber comprising a solid support. A nucleotide monomer is selected for addition to an oligonucleotide. An inlet stream comprising the selected monomer is fed to the reaction chamber (column) of the reaction chamber and conditions are provided in the reaction chamber for oligonucleotide synthesis. An outlet stream from the reaction chamber can be split and directed to the waste drain and/or to an analysis station where the eluent is analyzed by mass spectrometry (MS) to confirm the identity of the monomer fed through the inlet to the reaction chamber. Based on results from analyzing by MS, it is determined whether the outlet stream comprises the selected nucleotide monomer or a derivative thereof.

Abstract: A spectrometry system for spectroscopically analyzing a sample is provided. The system includes an excitation source for interacting with the sample; a detector for detecting at least a portion of light absorbed or emitted by the sample, the excitation source and detector being optically coupled via an optical pathway; and an aperture positioned in the optical pathway for limiting transmission of light from the excitation source to the detector; wherein the aperture is configured to have a spatially varying distribution of one or more geometric features that provide regions of variable transmission around an edge of the aperture. Also provided is a mask for use with a spectrometry system, the mask configured to be positioned in an optical pathway between an excitation source and a detector, wherein the mask has a spatially varying distribution of one or more geometric features that provide regions of variable transmission around an edge of the aperture.

Abstract: A shaft coupling system may include a first shaft including a disc that is engageable by a second shaft to retain the second shaft with the first shaft when the second shaft is rotated in a first direction, and release the second shaft from the first shaft when the second shaft is rotated in a second direction that is opposite to the first direction. A spring may be mounted to the first shaft to bias the second shaft towards the first shaft when the second shaft is retained with the first shaft. Further, a shaft coupling method may include engaging a second shaft with a disc of a first shaft by rotating the second shaft in a first direction relative to the first shaft, and disengaging the second shaft from the first shaft by rotating the second shaft in a second direction relative to the first shaft.

Abstract: A temperature control chamber includes a heat impact unit configured for heat impacting a sensor unit in accordance with a heat profile, the sensor unit configured for sensing heat impact response data over time in response to being heat impacted with the heat profile, and a determining unit configured for determining information indicative of a heat transfer characteristic in the temperature control chamber based on the sensed heat impact response data.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring carrier may be operatively disposed between the screw drive and the screw, and movable along an axis of the spring carrier by the screw drive to impart, by the screw, a sealing axial force to seal a capillary tip of a capillary against a port ground of the port.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring-loaded disc may be operatively disposed between the screw drive and the screw, and rotatable by the screw drive to impart, by the screw, a sealing axial force to seal a capillary tip of a capillary against a port ground of the port.

Abstract: In alternative embodiments, provided are immunohistochemistry (IHC) methods and kits for determining and scoring reproducibly the extent of expression of the protein tyrosine-protein kinase-like 7 (PTK7), also known as: colon carcinoma kinase 4 (CCK4); HER2 or receptor tyrosine-protein kinase erbB-2, or cluster of differentiation 340 (CD340); programmed death-ligand 1 (PD-L1), or cluster of differentiation 274 (CD274); B7 homolog 1 (B7-H1); and, Ki-67 or MKI67 (Marker of Proliferation Ki-67), in a tissue sample. In alternative embodiments, provided are methods and kits for diagnosing or selecting an individual eligible for treatment with a cancer or tumor therapeutic, or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein.

Abstract: In some examples, a capillary connection fitting may include a fitting housing that encloses a socket retainer and a spring. A spring compression limiter may include a specified length and is attached to the socket retainer to limit compression of the spring upon movement of the socket retainer to compress the spring.

Abstract: In some examples, a capillary connection fitting may include a screw drive, and a screw rotatable by the screw drive and insertable in a port. A spring-loaded disc may be operatively disposed between the screw drive and the screw, and axially movable by a socket of a capillary to impart, by the spring-loaded disc, a sealing axial force to seal a capillary tip of the capillary against a port ground of the port.

Abstract: Disclosed herein are methods and systems for evaluating the bioenergetic poise and bioenergetic capacity of living cells in a single assay.

Abstract: An apparatus having a waveguide and a freeform collimating lens. The waveguide is characterized by a waveguide axis and a planar end having a normal axis that is inclined at an end angle that is greater than 0 degrees with respect to the waveguide axis. The freeform collimating lens collimates light leaving the planar end of the waveguide into a collimated light beam characterized by a beam direction that is parallel to the waveguide axis. The apparatus inhibits reflections from the planar end of the waveguide from propagating back down the waveguide while providing a collimated light beam having a direction parallel to the axis of the waveguide.