Abstract: Peptides may be continuously produced by a method including the following steps (1), (A), (2), and (B): (1) performing a condensation reaction in a flow reactor to obtain an N-protected C-protected peptide in which an N-terminal amino group and C-terminal are protected by protecting groups, a side chain functional group is optionally further protected by a protecting group, and at least one of the C-terminal or the side chain functional group is protected by a pseudo-solid-phase protecting group (N-protected C-protected peptide), (A) washing a reaction mixture containing an N-protected C-protected peptide in a flow reactor and separating oil and water to separate an organic layer containing the N-protected C-protected peptide, (2) subjecting an organic layer containing an N-protected C-protected peptide to a reaction to remove a protecting group of an N-terminal amino group in a flow reactor to obtain a C-protected peptide in which an N-terminal amino group is not protected, C-terminal is protected by a pr

Abstract: Provided herein are drinks or powdered drinks containing a destroyed frond of a Lemnoideae plant having improved feeling of sliminess and/or powderiness in the mouth, and a method for improving sliminess and/or powderiness in the mouth of drinks or powdered drinks containing destroyed fronds of a Lemnoideae plant. Also provided herein are drinks or powdered drinks containing destroyed fronds of a Lemnoideae plant, and organic acid salt or organic acid; and a method for improving the feeling of sliminess and/or powderiness in the mouth, including adding an organic acid salt or organic acid to drinks or powdered drinks containing destroyed fronds of a Lemnoideae plant.

Abstract: Provided herein is composition containing a destroyed frond of a Lemnoideae plant with a suppressed off-flavor, and a method for suppressing an off-flavor of a destroyed frond of a Lemnoideae plant. A composition containing a destroyed frond of a Lemnoideae plant and also containing an organic acid salt, an inorganic salt, a sweetener, and/or an acidulant; and a method for suppressing an off-flavor, including blending an organic acid salt, an inorganic salt, a sweetener, and/or an acidulant with a destroyed frond of a Lemnoideae plant, are provided herein.

Abstract: A method including obtaining a phosphite form or a phosphorous acid diester form by coupling a nucleoside, nucleotide or oligonucleotide wherein a 5?-hydroxy group is not protected, and other group is optionally protected by a protecting group used for nucleic acid synthesis or bonded to a solid phase carrier, and a nucleoside, nucleotide or oligonucleotide wherein a 3?-hydroxy group or a 3?-amino group is modified by a method for forming a phosphite form or a phosphorous acid diester form, and other group is optionally protected by a protecting group used for nucleic acid synthesis in the presence of an antioxidant is useful for producing an oligonucleotide having a phosphorothioated site.

Abstract: A method is described for producing an L-amino acid including the steps of cultivating in a culture medium an L-amino acid-producing bacterium belonging to the family Enterobacteriaceae to produce and accumulate the L-amino acid in the culture medium, cells of the bacterium, or both, and collecting the L-amino acid from the culture medium, the cells, or both, wherein said bacterium has been modified to overexpress a gene encoding a periplasmic adaptor protein.

Abstract: Mouth-coating feel enhancers containing a heated substance of the following (A) and/or a heated substance of the following (B), or at least one compound selected from compound group (C) described in the present specification: (A) (A1) a compound represented by the formula (I): wherein each symbol is as defined in the present specification, and (A2) at least one selected from the group consisting of an aliphatic aldehyde having 3 to 14 carbon atoms, an aromatic aldehyde having 7 to 12 carbon atoms, an aliphatic alcohol having 3 to 12 carbon atoms, and a substance that produces at least one of these compounds by heating (B) at least one compound selected from the group consisting of ?-caryophyllene and a ?-caryophyllene analogous compound are effective as mouth-coating feel enhancers and coffee-roasting sensation enhancers.

Abstract: A method for producing allolactose is provided. Allolactose is produced by bringing cells of a microorganism having beta-galactosidase (BGL) into contact with lactose in the presence of a substance that inhibits hydrolysis of allolactose.

Abstract: Producing an isoprenoid compound by: 1) culturing an isoprenoid compound-forming microorganism in the presence of a growth promoting agent at a sufficient concentration to grow the isoprenoid compound-forming microorganism; 2) decreasing a concentration of the growth promoting agent to induce formation of the isoprenoid compound by the isoprenoid compound-forming microorganism; and 3) culturing the isoprenoid compound-forming microorganism to form the isoprenoid compound, is characterized in that the growth phase of the isoprenoid compound-forming microorganism is separated from the formation phase of the isoprenoid compound.

Abstract: A method for producing an L-amino acid such as L-lysine is provided. An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a carotenoid biosynthesis enzyme is increased, in a medium, and collecting the L-amino acid from the medium.

Abstract: A method for producing an oligonucleotide, including a step of removing a temporary protecting group of a 5?-hydroxyl group by adding an acid to a protected oligonucleotide having a phosphorothioate (PS), phosphate (PO) or H-phosphonate moiety, and deacylating a by-product having an acylated 5?-hydroxyl group by solvolysis can complete a condensation reaction to improve a condensation yield, and can markedly reduce impurities such as single base deletion form (N-1mer) and the like.

Abstract: A resin composition including (A) an epoxy resin, (B) a curing agent, and (C) an inorganic filler, in which a chloride ion content included in the resin composition measured in accordance with a sample combustion ion chromatography method (BS EN 14532 2007) is 50 ppm or less.

Abstract: Liquid cleansing agents with a low viscosity, containing (A) one or more kinds selected from the group consisting of N-acyl acidic amino acid and a salt thereof, (B) one or more kinds selected from the group consisting of N-acyl neutral amino acid and a salt thereof, and (C) an amphoteric surfactant can be filled in a foamer container and are cleansing agents with high cleansing power which afford fine foam discharged from a foamer container, maintain fineness, provide a good amount of lather and a smooth feeling after washing, can prevent clogging in a foamer container during use at low temperatures, and are also superior in low temperature stability.

Abstract: Adding (A) an amylomaltase derived from a bacterium of the genus Thermus to a starch-containing material, and further adding (B) a protein or lipid modification enzyme, or (C) a starch degradation product or (D) a starch degradation enzyme is useful for modifying a starch-containing food.

Abstract: A method for producing an objective substance such as sphingoid bases and sphingolipids using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance in a culture medium containing an additive that is able to associate with, bind to, solubilize, and/or capture the objective substance, and collecting the objective substance from cells of the yeast and/or the culture medium.

Abstract: Feed additive compositions for ruminants containing (A) at least one member selected from the group consisting of hydrogenated vegetable oil and hydrogenated animal oil each having a melting point of higher than 50° C. and lower than 90° C.; (B) not less than 0.05 wt % and not more than 6 wt % of lecithin; (C) not less than 30 wt % and less than 65 wt % of a biologically active substance; (D) not less than 0.01 wt % and less than 0.8 wt % of a natural vegetable oil; and not less than 0.1 wt % and less than 6 wt % of water exhibit high protection in the rumen and are also superior in dissolution in the lower gastrointestinal tract.

Abstract: The present invention provides a means capable of efficiently manufacturing a fused substance of an Fc protein and a substance of interest (for example, a peptide). Specifically, the present invention provides an azide group-containing Fc protein represented by formula (1): N3-La-Phe-Lb-Fc??(1) wherein N3 represents an azide group; La represents a bond or a divalent group; Phe represents a residue of phenylalanine or a derivative thereof; Lb represents a lysine residue or an arginine residue, or a peptide linker containing two or more amino acid residues having a lysine residue or an arginine residue at/on the N-terminus; and Fc represents an Fc protein; and the like.

Abstract: The present invention provides a method for producing a basic L-amino acid such as, for example, L-ornithine L-citrulline, L-arginine, L-histidine, and L-lysine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding a protein having L-methionine/branched-chain amino acid exporter activity.

Abstract: A mutant glutathione synthetase (GSHB) suitable for generating ?-Glu-Val-Gly, and a method for producing ?-Glu-Val-Gly using the same are provided. ?-Glu-Val-Gly is produced by using a mutant GSHB having a mutation at such a position as V7, N13, I14, N15, K17, F22, F95, M165, N199, Y200, P202, I274, T285, and P287.

Abstract: Muscle quality-improving agents containing one or more selected from the group consisting of isoleucine, glycine, and cystine can prevent a decline in muscle quality, improve muscle quality even when exercise is limited, and can effectively enhance the effect of exercise even when the exercise is of a level free of undue efforts.

Abstract: Ligase mutants of the following (1), (2), or (3): (1) a ligase mutant comprising an amino acid sequence showing 95% or more identity to the amino acid sequence of SEQ ID NO: 1, and having a nucleic acid-linking activity; (2) a ligase mutant comprising an amino acid sequence showing 90% or more identity to the amino acid sequence of SEQ ID NO: 2, and having a nucleic acid-linking activity; or (3) a ligase mutant comprising an amino acid sequence showing 97% or more identity to the amino acid sequence of SEQ ID NO: 3, and having a nucleic acid-linking activity, have excellent properties.