Abstract: The present invention relates to a method of manufacturing an analytical tool, where the method includes a reagent member forming process for preparing a reagent member containing a reagent to react with a specific component contained in sample liquid. The reagent member forming process includes a plurality of applying and drying steps in which material liquid containing a reagent is applied and then the material liquid is dried. The applying and drying steps may be performed with use of material liquid containing the same reagent, for example. The applying and drying steps are performed 2-200 times, for example.

Abstract: A predetermined voltage is applied to a biosensor twice to promote an electrochemical reaction, and the following parameters (P1 and P2) are calculated from the values of detected current. A statistical technique is used with these parameters to compensate for errors so that the concentration of an object can be determined. P1: the ratio (If/Ib) of the maximum current (If) or a current occurring the maximum in the first excitation to a current (Ib) read at any point in the second excitation. P2: a current (Ib) read at any point in the second excitation.