Abstract: An electrophoretic system employing a capillary with at least one end having an electrically conductive coating or layer directly thereon. In order to introduce a plug of sample from a small amount of the sample in a vial, the conductive tip is submersed into the small amount of sample and an electric field is applied at the end by applying the high voltage to the tip in order to electrokinetically inject a plug of the sample. The conductive tip may also be used as a terminal for completing the circuit for applying the high voltage across the capillary column for electrophoresis. The separated components may be collected on a surface or small amount of buffer to reduce sample dilution or mixing of one sample component with a different sample component from the electrophoretic process.

Abstract: Methods for modulating the rates and dose responses of immunoassays through the incorporation of one or more detergents into the immunoassay reaction are disclosed. The methods are particularly suitable for automated immunoassay formats, especially with formats that use analyte-biotin bidentate reagents. The methods may be used to facilitate the detection of any desired, preselected pharmacological agent.

Abstract: Deoxyribonucleotide and ribonucleotide derivatives of the general formula I ##STR1## wherein R.sup.1 represents --CR'R"--Ar, in which Ar is substituted aryl (as hereinafter defined) and R' and R" are independently selected from the group consisting of hydrogen and lower alkyl; one of --R.sup.2 and R.sup.3 is a hydroxyl-protecting group and the other is a group suitable for synthesis of polynucleotides or for attachment of the nucleotide to a solid support; R.sup.4 is selected from the group consisting of hydrogen, --OH and protected hydroxyl; and B represents a divalent radical corresponding to a purine or pyrimidine base. When synthesis is carried out using these derivatives, the deprotection procedure is reduced to an essentially instantaneous process. The derivatives have acceptable shelf life and are very stable to conventional DNA synthesis conditions. Particularly preferred are those compounds wherein Ar is mono- and dihalo-substituted phenyl.

Abstract: A sample segment including a body and wells formed therein, selected wells including a flat exterior surface surrounding a tapered end. The sample segment may retain selected reagents, and a sealing cover is held by ribs, stretched and pressed against raised bosses formed around the well openings to provide a sure seal. The bosses are discontinuous between adjacent wells. Selected wells may contain a free mixing element for enhanced mixing. The segment is uniquely adapted for automated handling and processing.

Abstract: Disclosed herein are protecting groups for exocyclic amino groups of the base cytosine for use in the synthesis of oligonucleotides and oligonucleoside phosphorothioates, the protecting groups being represented by the formula: --CO--(CH.sub.2).sub.0-9 --CH.sub.3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (--CO--CH.sub.3), and the oligonucleotide or oligonucleoside phosphorothioate incorporating the protected cytosine is subjected to a cleavage/deprotection reagent comprising methylamine and ammonia.

Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized.

Abstract: An analytical system uses a waveguide having embedded Bragg gratings to redirect light from the waveguide outwardly for analytical determination by detectors. The analytical system can be a spectrophotometer, spectrofluorimeter, or other means for analyzing the components of light passed through a sample or emanating from a sample. The system can be single or multi-channeled and can be integrated with a detector onto a single chip device. A manifold with multiple input waveguides and multiple output waveguides is also provided. The interaction point between the input and output waveguides is a target area in which a sample capillary tube can be located.

Abstract: A capillary and capillary retaining system including a capillary assembly having first and second end holders. The first and second end holders are adapted to be received by first and second retainers. The first end holder may include protruding portions and the first end retainer includes clips or clamps to engage the protruding portions. The second end holder may include opposite recesses and the second retainer is adapted to receive optical cables that are received within the recesses to retain the second end holder. The second retainer can further include a lever on the retainer body to pry the second holder out of the second retainer. Locks or clips retained by the second end retainer cooperate with grooves in the optical cables to retain the optical cables.

Abstract: Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface having an interconnected polymeric coating. The interconnected polymeric coating includes a hydrophobic hydrocarbon polymeric functionality covalently bound to the interior surface and polyvinylalcohol interconnected with the hydrophobic polymeric functionality. Exemplary columns are prepared by causing a Si-OH reactive compound, having a hydrophobic polymeric functionality, to react with Si-OH functionalities on the interior surface of capillary columns. Then causing vinyl acetate to polymerize in contact with the hydrophobic polymeric functionality and hydrolyzing the resulting polyvinylacetate forms a coating of interconnecting hydrophilic and hydrophobic polymers.

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract: A variable inclination centrifugation or rotor assembly and a method for rapid separations. The samples, such as whole blood, are centrifuged within pivoting tube holders while in a first position wherein the longitudinal axes of the tube holders are substantially parallel to a central axis of rotation. As rotation continues, the tube holders are inclined in a radial direction by controlled adjustment of the tube holders toward a second position wherein the axes of the tube holders and the rotation axis are substantially perpendicular. The first embodiment of the assembly employs a worm gear to incline the samples and allows for inclination and rotation of the samples to be controlled independently. The second and third embodiments employ a spring-loaded linkage system and a governor mass and three-arm linkage system, respectively, for inclination. These are activated by the rotational speed of the samples.

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

Abstract: An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium's turbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including, haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.

Abstract: A process for synthesizing oligonucleotides by phosphoramidite chemistry wherein the improvement is the use of substituted aryl carboxylic acids as the activators. These activators produce in situ nucleotide intermediates in which the substituted arylcarbonyl group has displaced the amidite moiety.

Abstract: Homogeneous immunoassays and enzyme-substrate assays which use capillary electrophoresis and fluoescent detection systems are described. The method is useful for detecting and/or quantitating the concentration of analytes in a sample.

Abstract: Processes for the electrophoretic analysis of monosaccharides and/or polysaccharides are disclosed. The processes generally first include providing a composition having derivatized mono and/or polysaccharide components which are monosaccharide and/or polysaccharide labelled with a fluorescing charged compound. Then introducing the composition into an electrophoretic medium and applying an electric field across the electrophoretic medium causes the derivatized monosaccharide and/or polysaccharide components to differentially migrate within the electrophoretic medium. Then detecting the derivatized monosaccharide and/or polysaccharide components is accomplished by laser exciting the derivatized monosaccharide and/or polysaccharide components and monitoring fluorescent emission of the derivatized monosaccharide and/or polysaccharide components. The processes provide extremely low detection limits of the labelled components.

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated biaxially oriented polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

Abstract: Nucleic acid probes and protein probes are disclosed. The nucleic acid probe comprises a probe polynucleotide, a charged hapten label, and a binding moiety. The protein probe comprises a probe protein, a charged hapten label and a binding moiety. The charged hapten label joint to the binding moiety can comprise a negatively charged sulfophenylhydrazine tag compound. Polyclonal antibodies and monoclonal antibodies with specific affinity for the charged hapten labels are disclosed as are hybridomas capable of making the monoclonal antibodies. Methods and kits are disclosed for making the nucleic acid probes, making the protein probes, detecting a capture polynucleotide of the nucleic acid probe and detecting a capture molecule of the protein probe.

Abstract: A heated cover for a receptacle containing a vaporizable substance. The cover is heated to a temperature above the temperature of the substance so as to prevent condensation of vapor evaporated from the substance. A device for placing and removing the cover with respect to the receptacle is designed in connection with a temperature-controlled heating/cooling plate which controls the temperature of the contents of the receptacle.

Abstract: A solid state ion-selective electrode device to detect a selected ion species in solution an electrode consisting of a homogeneous solid mixture. The mixture is a solid electrolyte composition possessing ionic conductivity and polyvinyl chloride, and optionally graphite. The electrode is substantially free of metallic silver. An electrical connection operates as a contact between the electrode device and a reference source. Methods for detecting the selected ion species in solutions, such as biological samples utilizing the above described electrodes, are also disclosed.