Abstract: The present invention relates to a method for in vitro diagnosis or prognosis of colon cancer, including a step of detecting at least one expression product of at least one HERV nucleic acid sequence, the use of said isolated nucleic acid sequences as a molecular marker/molecular markers, and a kit including at least one specific binding partner for at least one expression product of the HERV nucleic acid sequences.

Abstract: The present invention relates to a method for the in vitro diagnosis or prognosis of ovarian cancer, which includes a step of detecting at least one expression product of at least one HERV nucleic acid sequence, the use of said nucleic acid sequences, once isolated, as one or more molecular marker(s) and a kit including at least one specific binding partner of at least one of the expression products of the HERV nucleic acid sequences.

Abstract: A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Abstract: The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprises a fluid channel network disposed in both the first surface and the second surface and connecting the fluid intake port to the sample wells, the fluid channel network comprising at least one distribution channels, a plurality of fill channels operatively connected to the at least one distribution channel, a plurality of through-channels operatively connected to one or more of the fill channels and a plurality of horizontally orientated fill ports operatively connecting the fill channels to the sample wells.

Abstract: A method for detecting a biological species of interest (SOI) potentially present in an analysis sample, the biological species of interest having a known or partially known genome, the analysis sample comprising a mixture of various biological species, the method comprising (a) extracting nucleic acids from the analysis sample; (b) sequencing nucleotide sequences extracted, (c) on the basis of the sequencing result, (i) assigning the sequences resulting from the sequencing, based on a reference database of sequences; (i) assigning sequences; (ii) determining a quantity (RSOI, RNSOI) of sequences assigned to the biological species of interest; and, prior to the sequencing, adding a calibrator, the calibrator being a biological species added in a known concentration, to the analysis sample, the calibrator having a known genome, and on the basis of the sequencing result, determining a quantity (RCAL) of sequences assigned to the calibrator; (d) on the basis of the quantities of sequences estimated in the det

Abstract: A method for detecting a biological species of interest (SOI) present in an analysis sample, the biological species of interest having a known genome, the analysis sample comprising a mixture of various biological species, the method comprising (a) extracting nucleotide sequences from the analysis sample; (b) sequencing the nucleotide sequences extracted in the extracting; (c) on the basis of the sequencing, obtaining normalized sequences assigned to the biological species of interest and to the control species; and (d) comparing the normalized sequences respectively assigned to the biological species of interest and to the control species to detection thresholds respectively associated with the biological species of interest and with the control species.

Abstract: A method for collecting microorganisms when contained in a fluid includes (i) introducing the fluid into a cavity of a collecting device via at least one admission duct, (ii) capturing the microorganisms when contained in the fluid with a set of beads retained in the cavity as the fluid passes through the set of beads, (iii) evacuating the fluid from the cavity via at least one evacuating duct, (iv) introducing a reaction liquid into the cavity via at least one admission channel, (v) collecting the microorganisms from the set of beads with the reaction liquid as the reaction liquid passes through the set of beads, and (vi) evacuating the reaction liquid from the cavity via at least one evacuating channel.

Abstract: A kit for in vitro measurement of a at least one IL7R gene transcript in a blood sample, including specific reagents for measuring the transcript, and a control sample calibrated to contain the IL7R gene transcript corresponding to the mean quantity measured in a pool of reference blood samples from human patients in a state of septic shock when reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and who were known to have survived, and/or a calibrated to contain the quantity of an IL7R gene transcript corresponding to the mean quantity measured in reference blood samples from patients in a state of septic shock when the reference blood samples are taken, or who were in a state of septic shock within 72 h after taking the reference blood samples, and not to have survived.

Abstract: A method for determining a quantity Ginhib quantifying the inhibitory capacity of a molecule on a type of microorganism includes: preparing a plurality of samples, including microorganisms of the type, a nutrient medium for the microorganism and an initial amount of the molecule per microorganism increasing in a range [Qmin,Qmax] as a function of a classification of the samples; measuring the growth of the microorganisms in the samples as a function of time; and determining the quantity Ginhib as a function of the measurements of the growth. Determination of the quantity Ginhib includes: for each sample, calculating a value reflecting the growth of the microorganism of said type based on measurements of growth; classifying the values calculated for the samples as a function of the classification of the samples; and determining the quantity Ginhib as a function of the variation of the classified values.

Abstract: A new compound exhibiting particularly advantageous antibacterial properties, and having the structural formula: L-pyroglutamyl-L-1-aminoethyltetrazole (or N-(1-(1H-tetrazol-5-yl)ethyl)-5-oxopyrrolidine-2-carboxamide). Also, the use of this compound in microbiological culture methods and media intended for the detection, identification, enrichment, counting and/or isolation of target microorganisms that may be encountered in samples taken for clinical diagnosis or microbiological health control purposes.

Abstract: A detection of the Gram type of a bacterial strain includes: illumination, in the wavelength range 415 nm-440 nm, of at least one bacterium of said strain having a natural electromagnetic response in said range; acquisition, in the range 415 nm-440 nm, of a light intensity reflected by, or transmitted through, said illuminated bacterium; and determination of the Gram type of the bacterial strain as a function of the light intensity acquired in the range 415 nm-440 nm.

Abstract: Automated test instruments electronically determine and/or obtain cell availability of cells of a holding structure in an incubated test chamber and for each of a plurality of open and available cells and electronically identify neighboring cells, electronically determining whether each of the identified neighboring cells are occupied or empty and, if occupied, electronically evaluating at least one of a test status or a time from load of a specimen container held therein. The instruments then electronically rank each of the plurality of open and available cells based at least in part on whether the identified neighboring cells are occupied or empty and the at least one of test status or time from load of the occupied cells of the identified neighboring cells. The loading mechanism is then directed to load an incoming specimen container into a selected one of the open and available cells based on the ranking.

Abstract: The invention provides for methods and systems involving the measurement of values of one or several characterizing parameters representative of the kinetics of an assay chemical reaction, including providing a calibration tool (26) comprising a reaction chamber (28); for a given set of defining features, performing a series of calibration experiments under different sets of calibration values of at least one operating parameter, providing a digital calibration model representative of the kinetics of the assay chemical reaction in the calibration tool, where the assay digital calibration model comprises one or several characterizing parameters, the values of which have a dependency on the given set of defining features used for the calibration experiments; fitting, by computation, the values of the characterizing parameters for the given set of defining features, based on the series of calibration experiment results, wherein the reaction chamber (28) is a stirred-tank reactor.

Abstract: A method of calibration of a device for analyzing at least one element present in a sample, said device including: a detection assembly configured to acquire an image formed by the interference between a light source and said sample; and digital processing means configured to detect a digital position of at least one element in said sample based on said acquired image; said calibration method including the implementation of a plurality of predetermined displacements of said sample with respect to said detection assembly and, for all of said displacements, the detection of a digital position of a same element to determine the digital position and the real position matching model according to the predetermined displacements and to the digital positions of said element after each displacement.

Abstract: A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Abstract: The subject of the invention is a method for making an in vitro prognosis of severity for a patient in septic shock, including the following steps: (i) the level of expression of the expression product of at least one gene chosen from the lilrb2 and lilrb1 genes is measured in vitro on the basis of a biological sample taken from the patient, (ii) the level of expression of the expression product of the at least one gene is compared with a control level of expression, of the expression product of the same gene, with a good prognosis of severity, in which a level of expression of the expression product of the at least one gene below the control level of expression indicates a poor prognosis of severity for the patient, and also a kit for implementing the method.

Abstract: An obturation element (1) for a well receiving a chemical or biological solution to be analyzed, including for each well, a plug (4) including an insertion sleeve (5) in the form of a cylinder provided with two truncated areas (15) arranged in a symmetrically opposite manner on the outside of the sleeve to form vents, this insertion sleeve (5) being provided with a bottom wall (9) in which a through-cutout (18) is arranged, oriented radially in the direction of the two truncated areas (15).

Abstract: A device for determining contamination of a fluid by microorganisms has a housing with an internal volume, a cover closing the housing, a fluid inlet port, at least one filtration member, at least one nutrient layer including a composition of a microbiological culture medium, characterized in that the device includes a fluid outlet port, and in that the cover has an inner surface in the internal volume extending radially about the fluid inlet port up to a peripheral edge of the cover, said inner surface being inclined and converging towards the fluid inlet port, and in that the bottom of the housing has a surface extending radially about the fluid outlet port up to the side wall of the housing, said inner surface being inclined and converging towards the fluid outlet port.

Abstract: The invention concerns a method of in vitro or ex vivo evaluation of the risk of complications in a patient who has sustained an insult or an infection generating a systemic inflammatory response syndrome, the method being characterized in that it comprises the step of detecting, in a biological sample obtained from said patient, at least one transcript of the IL7R gene, as well as measuring, in vitro or ex vivo, the quantity of at least one transcript of the IL7R gene, in a biological sample from a patient who has sustained an insult or an infection generating a systemic inflammatory response syndrome, in order to evaluate the risk of complications, and in particular of mortality, in said patient. The invention also concerns kits for measuring, in vitro or ex vivo, the quantity of at least one transcript of the IL7R gene in a biological sample.