Abstract: The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and/or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.

Abstract: Methods for modeling cancer cell migration, screening drugs for effects on tumor cell migration, and detecting the potential for tumor cell migration relating to the fusion of a bone marrow derived stem cell with a genetically altered cell. Antibodies against ubiquitin are shown to inhibit tumor cell migration.

Abstract: An apparatus and methods for characterizing the response of a particle to a parameter that characterizes an environment of the particle. A change is induced in the parameter characterizing the environment of the particle, where the change is rapid on a timescale characterizing kinetic response of the particle. The response of the particle is then imaged at a plurality of instants over the course of a period of time shorter than the timescale characterizing the kinetic response of the particle. The response may be detected by measuring a temperature jump or by measuring correlation and anticorrelation between probe parameters across pixels. More particularly, the particle may be a molecule, such as a biomolecule, and the environment, more particularly, may be a biological cell. The parameter characterizing the environment of the particle may be a temperature, and change may be induced in the temperature by heating a volume that includes the particle, either conductively or radiatively.

Abstract: Methods and compositions for reducing viral genome amounts in a target cell are provided. In the subject methods, the activity of a miRNA is inhibited in a manner sufficient to reduce the amount of viral genome in the target cell, e.g., by introducing a miRNA inhibitory agent in the target cell. Also provided are pharmaceutical compositions, kits and systems for use in practicing the subject methods. The subject invention finds use in a variety of applications, including the treatment of subjects suffering from a viral mediated disease condition, e.g., an HCV mediated disease condition.

Abstract: Methods are provided for treatment of hematologic cancers, particularly lymphomas and leukemias, including without limitation myelogenous and lymphocytic leukemias. A combination of antibodies specific for CD47; and specific for a cancer associated cell surface marker are administered to the patient, and provide for a synergistic decrease in cancer cell burden. The combination of antibodies may comprise a plurality of monospecific antibodies, or a bispecific or multispecific antibody. Markers of interest include without limitation, CD20, CD22, CD52, CD33; CD96; CD44; CD123; CD97; CD99; PTHR2; and HAVCR2.

Abstract: A composite material includes a matrix composed of a polyhydroxyalkanoate (PHA) polymer and a filler composed of particles dispersed in the matrix. The particles are composed of naturally-derived materials (e.g., ground bone meal or pumice powder), have a microporous microstructure, have a low hygroscopic expansion, and are less than 1.0 mm in size. Preferably, the matrix and the filler together constitute 100% by weight of the composite material, and at most 30% by volume of the composite material is consumed by the filler. The composite material may take the form of an anaerobically biodegradable article of manufacture such as a building material a coating of a building material or other article.

Abstract: The present invention is directed to a method of regulating gastrointestinal action in a subject using a stimulatory electrode and a sensor to provide retrograde feedback control of electrical stimulation to the GI tract.

Abstract: Techniques and structures are disclosed in which one or more distortion categories are identified for an image or video, and a quality of the image or video is determined based on the one or more distortion categories. The image or video may be of a natural scene, and may be of unknown provenance. Identifying a distortion category and/or determining a quality may be performed without any corresponding reference (e.g., undistorted) image or video. Identifying a distortion category may be performed using a distortion classifier. Quality may be determined with respect to a plurality of human opinion scores that correspond to a particular distortion category to which an image or video of unknown provenance is identified as belonging. Various statistical methods may be used in performing said identifying and said determining, including use of generalized Gaussian distribution density models and natural scene statistics.

Abstract: The present invention relates generally to compositions and methods for producing lactic acid using a lactic acid producing microorganism. More specifically, the present invention relates to methods for producing lactic acid with high yield, high concentration and high volumetric productivity through biological fermentation by Enterococcus faecalis, or recombinant microorganisms transformed to produce lactate dehydrogenase using the lactate dehydrogenase-encoding genes derived from E. faecalis.

Abstract: In various embodiments methods are provided for identifying a mammal having an elevated risk for an adverse cardiac event (e.g. an MI) and/or determining the prognosis for the mammal. In certain embodiments the methods comprise determining, or causing to be determined, the presence and/or level of antibodies that bind a malondialdehyde-acetaldehyde adduct (MAA adduct) in a biological sample from the mammal, where an elevated level of anti-MAA adduct antibodies, as compared to the level found in a normal healthy mammal is an indicator that that said mammal has one or more atherosclerotic lesions and/or is at elevated risk for a myocardial infarction.

Abstract: Compounds relating to batrachotoxin are provided, in particular analogues that modulate the activity of sodium channels. Also provided are pharmaceutical compositions comprising compounds of the invention and a pharmaceutically acceptable carrier, including vehicles that modulate transdermal permeation of the compound. The subject compounds are useful in treatments, including treatments to reduce neuronal activity or to bring about muscular relaxation. The compounds also find use in the treatment of subjects suffering from a voltage-gated sodium channel-enhanced ailment or from pain. Further methods are provided for the preparation of the batrachotoxin-related compounds.

Abstract: Optical methods, devices, and systems for noninvasively detecting transient surface displacements in a neuron are disclosed. Methods, devices, and systems provided may employ a phase-sensitive optical low coherence reflectometer. In addition, surface displacements due to action potential propagation in neural tissues may be detected in some embodiments using back-reflected light. According to some embodiments, exogenous chemicals or reflect ion coatings are not required. Transient neural surface displacement of less then 1 nm in amplitude and 1 ms in duration may be detected and may be generally coincident with action potential arrival to the optical measurement site. The systems and methods may be used for noninvasive detection of various neuropathies such as retinal neuropathies. They may also be useful in detecting the effects of various pharmacological agents.

Abstract: A method of forming a porous sol gel, including a dried porous sol gel, is provided comprising forming a sol gel from a sol gel-forming composition comprising a silane solution and a catalyst solution; and non-supercritically drying the sol gel to provide a dried porous sol gel having no springback. The dried porous sol gel can include dried macroporous or mesoporous sol gels or dried hybrid aerogels. The materials may contain open or filled pores.

Abstract: An optimized heating and cooling system including a thermal mass, thermal energy transport conduits to deliver thermal energy to the thermal mass including one or more phase change materials (PCMs), at least one heat exchanger to exchange the thermal energy from a energy input into heat transfer fluid that is pumped through the thermal mass. The system also includes a controller in electronic communication with a temperature sensor, a throttle and a pump. A desired building temperature profile, a daily temperature forecast, the electricity rates, the thermal characteristics of the PCMs are entered into or obtained by the controller and the controller uses that information to optimize the energy use to avoid using the heating and cooling system during peak electricity demand time, or uses the rate structure to determine the operation sequence that results in the most efficient use of energy or least cost.

Abstract: A roof system includes a roof panel formed of concrete and an architectural surface such as a corrugated surface. A first layer of reinforcing material is disposed along and spaced inwardly from a first surface of the roof panel, and an optional second layer of reinforcing material is disposed along and spaced inwardly from a second surface of the roof panel. The first layer of reinforcing material is spaced from the second layer of reinforcing material. The reinforcing material may be formed of fiber-reinforced polymer mesh, made with carbon fibers or glass fibers. FRP rebar may be used in conjunction with FRP mesh to provide added structural strength. The roof panel may include one or more elongated ribs having a thickness greater than adjacent portions of the roof panel. In other aspects, concrete structures with reinforcing material may be used for either roof panels or wall applications.

Abstract: PARylated proteins are enriched by treating cell lysates comprising PARylated proteins and DNA/RNA with an endonuclease that cleaves the DNA/RNA but not the PAR; and separating the PARylated proteins from the cleaved DNA/RNA. PARylation sites are labeled by eluting PARylated proteins from a PAR-affinity substrate with a nucleophilic amine exchange reactant, wherein the reactant labels PARylation sites of the proteins. Specific binding agents are identified by screening compounds for specific binding to a PARylated protein disclosed herein; and identifying one of the compounds as a specific binder of the protein. Antibodies which specifically bind PARylation sites are also disclosed.

Abstract: A soybean cultivar designated KK-08 is disclosed. The invention relates to the seeds of soybean cultivar KK-08, to the plants of soybean cultivar KK-08, to the plant parts of soybean cultivar KK-08, and to methods for producing progeny of soybean cultivar KK-08. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar KK-08. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar KK-08, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar KK-08 with another soybean cultivar.

Abstract: A method of synthesizing carbon nanotubes. In one embodiment, the method includes the steps of: (a) dissolving a first amount of a first transition-metal salt and a second amount of a second transition-metal salt in water to form a solution; (b) adding a third amount of tannin to the solution to form a mixture; (c) heating the mixture to a first temperature for a first duration of time to form a sample; and (d) subjecting the sample to a microwave radiation for a second duration of time effective to produce a plurality of carbon nanotubes.

Abstract: A board mount which can be utilized to position a board relative to a building element. In various embodiments, the board mount can include a holder for receiving at least a portion of a drywall board and, in addition, a flexible member which can be inserted intermediate first and second building elements to retain the board holder relative to one of the first and second building elements. The flexible member can be configured to be deflected, or flexed, between a first configuration and a second configuration when the flexible member is positioned intermediate the building elements. In use, the flexible member can hold the board mount in place while a laborer inserts the drywall board into the board holder. In such circumstances, an additional laborer may not be required to hold the drywall board in position as it is fastened, or otherwise secured, to the frame of the building.

Abstract: The present invention relates to a portable apparatus for warming biocompatible fluids for use in the treatment of injured patients and a method of heating a biocompatible fluid to treat a patient experiencing hypothermia. The present invention may be used to warm intravenous fluids for trauma resuscitation or to warm air from a ventilator circuit. The portable nature of the present invention makes it highly suitable for field applications, such as a forward surgical hospital near a combat zone.