Patents Assigned to Caliper Life Sciences, Inc.
  • Patent number: 11235333
    Abstract: Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: February 1, 2022
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Morten J. Jensen, Andrea W. Chow, Colin B. Kennedy, Stephane Mouradian
  • Patent number: 11162910
    Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: November 2, 2021
    Assignees: CALIPER LIFE SCIENCES, INC., Canon U.S.A., Inc.
    Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Edward Donlon, Robert Moti, Michael Slater
  • Patent number: 11040118
    Abstract: The disclosure features methods that include: administering to a subject a composition that includes particles, where each one of the particles features at least one targeting group that binds to a structural entity in the subject and at least one reacting group that reacts chemically with a reactive oxygen species in the subject, and where the particle emits luminescence when the reaction occurs; detecting the luminescence emission from the particles; and displaying an image of the subject showing locations of at least some reactive oxygen species in the subject based on the detected luminescence.
    Type: Grant
    Filed: November 7, 2018
    Date of Patent: June 22, 2021
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Sunetra Ray, Daniel Ansaldi, Rajendra Singh
  • Patent number: 10877032
    Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.
    Type: Grant
    Filed: December 4, 2017
    Date of Patent: December 29, 2020
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrew Barry, Laurel Provencher, Seth Cohen, I-Jane Chen, Jun Yan, Jingjing Wang
  • Patent number: 10717081
    Abstract: The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: July 21, 2020
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Joshua I. Molho, Daniel G. Stearns, I-Jane Chen, Danh Tran, Bradley W. Rice, Tobias Daniel Wheeler
  • Patent number: 10648023
    Abstract: The invention provides systems and methods for processing samples. In a method, a reaction card is provided that has a channel network, a valve, and a micropump, all disposed within the card. The reaction card also has a collection well disposed on a surface of the card and a tubular member extending out from the card. A reaction vessel is provided and affixed to the reaction card such that the tubular member is inserted into the reaction vessel. Amplification reaction reagents and a sample are delivered into the reaction vessel, and an amplification reaction is initiated within the reaction vessel, resulting in an amplification product being disposed within the reaction vessel. The valve is opened to atmosphere, and the first micropump is activated to pump an aliquot of reaction product from the reaction vessel into the tubular member, through the channel network, and into the collection well.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: May 12, 2020
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Edward H. Thorne, IV, Abbie L. Esterman
  • Patent number: 10428377
    Abstract: Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: October 1, 2019
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Michael R. Knapp, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael Spaid
  • Patent number: 10138517
    Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
    Type: Grant
    Filed: June 6, 2017
    Date of Patent: November 27, 2018
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
  • Patent number: 10130318
    Abstract: An integrated microtomography and optical imaging system includes a rotating table that supports an imaging object, an optical stage, and separate optical and microtomography imaging systems. The table rotates the imaging object about a vertical axis running therethrough to a plurality of different rotational positions during a combined microtomography and optical imaging process. The optical stage can be a trans-illumination, epi-illumination or bioluminescent stage. The optical imaging system includes a camera positioned vertically above the imaging object. The microtomography system includes an x-ray source positioned horizontally with respect to the imaging object. Optical and x-ray images are both obtained while the imaging object remains in place on the rotating table. The stage and table are included within an imaging chamber, and all components are included within a portable cabinet.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: November 20, 2018
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Daniel G. Stearns, David G. Nilson, Bradley W. Rice
  • Patent number: 10126242
    Abstract: The disclosure features methods that include obtaining multispectral image information for a sample that includes a fluorescent dye, calculating from an image cube of the information a first spectrum and a second spectrum, and calculating a pure spectrum of the fluorescent dye in the sample based on the first and second spectra, where a relative contribution of light emission from the fluorescent dye to the second spectrum is larger than a relative contribution of light emission from the fluorescent dye to the first spectrum, where calculating the first and second spectra includes identifying corresponding first and second sets of pixel intensity values in the image cube, and where identifying the first set of pixel intensity values includes designating one or more layers of the image cube as a first layer set.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: November 13, 2018
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Peter J. Miller, Kent S. Johnson
  • Publication number: 20180321170
    Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
    Type: Application
    Filed: May 25, 2018
    Publication date: November 8, 2018
    Applicants: Canon U.S. Life Sciences, Inc., CALIPER LIFE SCIENCES, INC.
    Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
  • Patent number: 9987636
    Abstract: Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.
    Type: Grant
    Filed: April 20, 2015
    Date of Patent: June 5, 2018
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Morten J Jensen, Andrea W Chow, Colin B. Kennedy, Stephane Mouradian
  • Patent number: 9983155
    Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 29, 2018
    Assignees: Canon U.S. Life Sciences, Inc., CALIPER LIFE SCIENCES, INC.
    Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
  • Patent number: 9835623
    Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: December 5, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrew Barry, Laurel Provencher, Seth Cohen, I-Jane Chen, Jun Yan, Jingjing Wang
  • Patent number: 9770220
    Abstract: An integrated microtomography and optical imaging system includes a rotating table that supports an imaging object, an optical stage, and separate optical and microtomography imaging systems. The table rotates the imaging object about a vertical axis running therethrough to a plurality of different rotational positions during a combined microtomography and optical imaging process. The optical stage can be a trans-illumination, epi-illumination or bioluminescent stage. The optical imaging system includes a camera positioned vertically above the imaging object. The microtomography system includes an x-ray source positioned horizontally with respect to the imaging object. Optical and x-ray images are both obtained while the imaging object remains in place on the rotating table. The stage and table are included within an imaging chamber, and all components are included within a portable cabinet.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: September 26, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Daniel G. Stearns, David G. Nilson, Bradley W. Rice
  • Patent number: 9689029
    Abstract: The invention provides systems and methods for processing samples. In a method, a reaction card is provided that has a channel network, a valve, and a micropump, all disposed within the card. The reaction card also has a collection well disposed on a surface of the card and a tubular member extending out from the card. A reaction vessel is provided and affixed to the reaction card such that the tubular member is inserted into the reaction vessel. Amplification reaction reagents and a sample are delivered into the reaction vessel, and an amplification reaction is initiated within the reaction vessel, resulting in an amplification product being disposed within the reaction vessel. The valve is opened to atmosphere, and the first micropump is activated to pump an aliquot of reaction product from the reaction vessel into the tubular member, through the channel network, and into the collection well.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: June 27, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Edward H. Thorne, IV, Abbie L. Esterman
  • Patent number: 9683994
    Abstract: The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.
    Type: Grant
    Filed: July 9, 2012
    Date of Patent: June 20, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
  • Patent number: 9670541
    Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
    Type: Grant
    Filed: August 11, 2015
    Date of Patent: June 6, 2017
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
  • Patent number: 9604945
    Abstract: Derivatives of Sydnonimine and its analogues, which bind selectively to dopamine transporter (DAT) proteins are useful for treating and delaying the progression of disorders and illnesses that are alleviated by inhibiting dopamine reuptake.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: March 28, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Hao Chen, Ming Liu, Qi Su, Manish Raisinghani
  • Patent number: 9539574
    Abstract: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: January 10, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Charles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho