Abstract: An SACP method includes directing a beam of charged particles onto an object surface of an object using a particle optical system, and detecting intensities of particles emanating from the object. The method further includes: (a1) adjusting an excitation of the second beam deflector for adjusting an impingement location of the beam on the object surface; (a2) adjusting an excitation of the first beam deflector for adjusting an angle of incidence of the beam on the object surface without changing the impingement location and detecting the intensity; and (a3) repeating the adjusting of the excitation of the first beam deflector for adjusting the angle of incidence without changing the impingement location such that a corresponding intensity is detected for each of at least 100 different angles of incidence at the same impingement location.

Abstract: An alignment marker unit for an object holder for microscopic imaging of an object with a microscope, wherein the alignment marker unit can be removed from and remounted on the object holder, wherein the alignment marker unit has at least one alignment marker for microscope-supported detection, and wherein the alignment marker is designed to determine a coordinate system for the object holder which allows for the microscope-supported calibration of the object or an area of the object relative to its position to the object holder and the position of the object holder relative to the microscope. Furthermore, an object holder system including an object holder and an alignment marker unit as described above is provided.

Abstract: A microscope comprising multiple optical systems in the imaging beam path and at least one subassembly, the optical effect of which in relation to the imaging beam path can be modified by controlling the subassembly, e.g. a group of lenses or a diaphragm that can be moved in the direction of the optical axis, a diaphragm having a variable aperture, a digital zoom device, a shutter, or a focusing device. The microscope includes a control unit which is designed to generate control signals for the subassembly in a first mode of operation to regulate or control the functional parameters exclusively of the imaging optical system with which the subassembly is associated, and additionally in a second mode of operation to regulate or control the functional parameters of the entire optical system of the microscope.

Abstract: An apparatus for transmitted light illumination for light microscopes A diaphragm edge may be variably positioned in the direction of the optical axis, wherein a position of the diaphragm edge in the direction of the optical axis can be varied irrespectively of a position of the diaphragm edge transversely to the optical axis. A separate sample support table may be mounted on a housing. The housing has a passage opening, through which the diaphragm edge can be moved in the direction of the optical axis. A holding device is formed in the region of the passage opening of the housing or on a separate sample support table and a control device is present which is adapted to position the diaphragm edge in dependence at least upon a determined presence of a sample support table.

Abstract: A device for mass selective determination of at least one ion or of a plurality of ions is used, for example, in a measuring apparatus having an ion trap. The ion trap has a ring electrode having a first opening. A first electrode is arranged at the first opening. Furthermore, an amplifier for providing a radio-frequency storage signal for the ion trap and a first transformer are provided, said first transformer being connected to the amplifier and the first electrode in such a way that the radio-frequency storage signal is coupled into the first electrode via the first transformer.

Abstract: A method for operating a particle beam device and/or for analyzing an object in a particle beam device are provided. For example, the particle beam device is an electron beam device, an ion beam device, or a combination device having an electron beam device and an ion beam device. In various embodiments, the method steps of a so-called stereoscopy method and a multi-detector method may be combined with one another in such a manner that simple and rapid analysis of the object is made possible.

Abstract: An optical device for measuring luminescence includes a pulse generator for generating a periodic modulation signal having rectangular pulses, a pulse duration of the pulse being variably adjustable, an illumination device and/or means for illuminating an object under investigation with excitation radiation modulated in a pulse-like manner depending on the modulation signal, and a time-of-flight camera for phase-sensitive detection of a luminescence response emitted by the object under investigation in response to the excitation radiation. The modulation signal is supplied as reference signal to the time-of-flight camera.

Abstract: A process of preparing a lamella from a substrate includes manufacturing a protection strip on an edge portion of the lamella to be prepared from the substrate, and preparing the lamella, wherein the manufacturing the protection strip includes a first phase of activating a surface area portion of the substrate, and a second phase of electron beam assisted deposition of the protective strip on the activated surface area portion from the gas phase.

Abstract: A device for attaching a first optical microscope component part to a second part includes an annular receptacle attached to the second part and an annual insertion part. The annular insertion part includes outside-conical retaining projections. The receptacle includes a base ring having a retaining collar with an inside cone tapering away from the base ring and in which a lateral opening is formed, through which the insertion part can be inserted in the receptacle such that it is located on a pre-locking position in which the annular openings overlap. The insertion part and the receptacle can be moved by reciprocal rotation from the pre-locking position into a locking position in which the outside-conical retaining projections of the insertion part are seated against the inside cone of the retaining collar and press the insertion part on the base ring, whereby the insertion part is locked to the receptacle.

Abstract: An optical filter device having a polarizing beamsplitter, an achromatic polarization manipulator, and at least one dichroic mirror. The polarization manipulator is arranged optically between a first input/output of the polarizing beamsplitter and the dichroic mirror. The polarization manipulator is also constructed in such a way that it effects a 90-degree rotation of a polarization direction of light which a) exits the polarizing beamsplitter at the first input/output, b) traverses the polarization manipulator, c) is reflected by the dichroic mirror, and d) again traverses the polarization manipulator. The optical filter device can also be used as beamsplitter.

Abstract: The invention relates to a planapochromatically-corrected immersion microscope objective for high-resolution microscopy applications with changing dispersive immersion conditions, having a plurality of lenses and/or subsystems (T1, T2, T3) comprising lens groups and a corrective function (LA2) for eliminating spherical aberrations. According to the invention, the microscope objective has an additional corrective function (LA1) for eliminating longitudinal chromatic aberrations caused by dispersive changes in the immersion by changing the air gaps between the lenses or gap combinations, wherein the influence on the longitudinal chromatic aberration corresponds to a rotation of the curve s(?), which describes the color point (s) as a function of the wavelength (?).

Abstract: The invention allows a quantitative evaluation of images acquired by microscope having fewer errors and is applicable in connection with high-resolution methods, particular at a high speed. A microscope image is analyzed in which the intensity distributions of fluorescence events have in each instance a diffraction-dependent extent which corresponds to an extent of a point spread function of the microscope and are arranged so as to be spatially non-overlapping, or at least predominantly spatially non-overlapping, in that at least one counter is initialized for every region to be analyzed in the microscope image, at least one fluorescence event is identified in a region to be analyzed in the microscope image, and the counter corresponding to the relevant region is incremented for each fluorescence event identified in the region. The counting results in a dramatic improvement in the signal-to-noise ratio at a high evaluation speed.

Abstract: The present disclosure relates to a family of microscopes, each with at least one lens group that includes at least one lens and a lens mount and moves along the optical axis via a drive system for The drive system includes a rotary motor and transmission elements to convert the rotary motion to linear motion to transmit the translational movement to the lens group, and, simultaneously, to prevent rotations of the lens group about the optical axis. In an embodiment, the rotating output shaft of the motor is connected with a screw spindle that engages with a threaded hole machined into the lens mount, so that the lens group is directly moved in a translational manner. The engagement of the screw spindle with the lens mount both effects the translational movement of the lens group and secures the lens group against rotation about the optical axis.

Abstract: The invention relates to a device for microscopy, with at least one light source for providing illumination light, with a detection unit for detecting light radiated back from a sample, with a microscopy optical unit for guiding illumination light onto the sample and for guiding light radiated back from the sample in the direction of the detection unit and with, arranged in an illumination beam path, an excitation mask (40) for providing structured illumination. The device is characterized in that the excitation mask is a spatially structured filter, which is transparent to light with a first physical property and which impresses spatial structure onto light with a second physical property that is different from the first physical property. The invention moreover relates to a method for microscopy.

Abstract: The invention relates to an inverted microscope, including a microscope stand having a stand housing. The stand housing has a rear wall, a front wall, and side walls, and an objective turret, a focusing drive, and a rotatable retaining device, which is designed as a reflector turret and which has a plurality of locations for receiving exchangeable optical components. The retaining device protrudes from a side wall of the stand housing so that the optical components can be quickly changed without having to remove the retaining device from the stand housing.

Abstract: A system for supplying a process gas to a processing location of a particle beam system is disclosed. The system for supplying the processing gas includes a gas reservoir, a gas conduit, a pipe located close to the processing location, a valve between the gas conduit and the pipe, and a controller configured to open and to close the valve to switch the system from a first mode of operation in which process gas is not supplied to the processing location to a second mode of operation in which process gas is supplied to the processing location. The controller can alternately open and close the valve in cycles. Each cycle can include a first duration in which the valve is open and a second duration in which the valve is closed. The ratio of the first duration to the second duration can be changed.

Abstract: The invention is directed to an optical arrangement with a light source for emitting a light bundle and with optical elements for transforming this light bundle into the shape of a light sheet, particularly suitable for illuminating individual planes of a three-dimensional specimen in selective plane illumination microscopy (SPIM). According to the invention, means are provided for varying the cross section of the light sheet, for varying the length of the light sheet and/or for influencing the direction in which individual beam components extending within the light sheet are directed to the specimen substance. This makes it possible to adapt the geometry of the light sheet to the illumination requirements for observing one and the same specimen plane with a plurality of different objectives and, if required, to reduce shadows occurring within the observed specimen plane as a result of the illumination.