Patents Assigned to Cellomics, Inc.
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Patent number: 6759206Abstract: The present invention provides systems, methods, and screens to measure receptor internalization in a single step with appropriate automation and throughput. This approach involves luminescent labeling of the receptor of interest and the automated measurement of receptor internalization to a perinuclear location.Type: GrantFiled: July 12, 1999Date of Patent: July 6, 2004Assignee: Cellomics, Inc.Inventors: Richard A. Rubin, Kenneth A. Giuliano, Albert H. Gough, R. Terry Dunlay
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Patent number: 6756207Abstract: The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions.Type: GrantFiled: October 29, 1999Date of Patent: June 29, 2004Assignee: Cellomics, Inc.Inventors: Kenneth A. Giuliano, Gary Bright, Keith Olson, Sarah Burroughs Tencza
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Publication number: 20040101912Abstract: The present invention provides systems, methods, and screens to measure receptor internalization in a single step with appropriate automation and throughput. This approach involves luminescent labeling of the receptor of interest and the automated measurement of receptor internalization to a perinuclear location.Type: ApplicationFiled: October 15, 2003Publication date: May 27, 2004Applicant: Cellomics, Inc.Inventors: Richard A. Rubin, Kenneth A. Giuliano, Albert H. Gough, R. Terry Dunlay, Bruce Ray Conway
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Patent number: 6727071Abstract: The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is microplate having cells in a micropaterned array of locations.Type: GrantFiled: December 8, 1999Date of Patent: April 27, 2004Assignee: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor, Albert H. Gough, Kenneth A. Giuliano
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Patent number: 6716588Abstract: The present invention provides automated systems, methods, screens, and software for the analysis of cell spreading. The invention involves providing cells containing fluorescent reporter molecules in an array of locations, contacting the cells with a test stimulus, acquiring images from the cells, and automatically calculating one or more morphological features that provide a measure of cell spreading.Type: GrantFiled: December 8, 2000Date of Patent: April 6, 2004Assignee: Cellomics, Inc.Inventors: Paul Sammak, Thomas D. Duensing, Richard A. Rubin
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Publication number: 20040063162Abstract: The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations.Type: ApplicationFiled: October 15, 2003Publication date: April 1, 2004Applicant: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor, Albert H. Gough, Kenneth A. Giuliano
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Patent number: 6671624Abstract: The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations.Type: GrantFiled: November 27, 2000Date of Patent: December 30, 2003Assignee: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor, Albert H. Gough, Kenneth A. Giuliano
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Publication number: 20030204316Abstract: The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations.Type: ApplicationFiled: May 6, 2003Publication date: October 30, 2003Applicants: Cellomics, Inc., BioDx, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor
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Patent number: 6620591Abstract: The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.Type: GrantFiled: April 16, 1999Date of Patent: September 16, 2003Assignee: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor
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Publication number: 20030104479Abstract: The present invention provides novel recombinant fusion proteins for detecting binding of a molecule of interest containing a detection domain, a first and optionally a second localization domain, and a binding domain. The invention also provides recombinant nucleic acid molecules and recombinant expression vectors encoding these novel fusion proteins, genetically engineered host cells containing these expression vectors, and kits for the use of these fusion proteins, nucleic acid molecules, expression vectors, and host cells. Additionally, the present invention provides methods for identifying compounds that alter the binding of a molecule of interest in a cell.Type: ApplicationFiled: August 1, 2002Publication date: June 5, 2003Applicant: Cellomics, Inc.Inventors: Gary Bright, Daniel Rajadavid Premkumar, Yih-Tai Chen
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Patent number: 6573039Abstract: The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.Type: GrantFiled: August 29, 2000Date of Patent: June 3, 2003Assignee: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor
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Publication number: 20030096322Abstract: The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions.Type: ApplicationFiled: March 19, 2002Publication date: May 22, 2003Applicant: Cellomics, Inc.Inventors: Kenneth Giuliano, Ravi Kapur
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Patent number: 6548263Abstract: The present invention describes novel methods for making a substrate for selective cell patterning, and the substrates themselves, wherein the method comprises contacting reactive hydroxyl groups on the surface of a substrate with a hydroxyl-reactive bifunctional molecule to form a monolayer, and using stencils to deposit cell repulsive or cell adhesive moieties in controlled locations on the cell culture substrate. Methods comprising selective differentiation of stem cells to create tissue specific and organ-specific cell substrates, as well as the cell substrates themselves are also provided.Type: GrantFiled: March 31, 2000Date of Patent: April 15, 2003Assignee: Cellomics, Inc.Inventors: Ravi Kapur, Terri Adams
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Publication number: 20030059093Abstract: The present invention provides automated methods and associated software for determining the organization of a component of interest in individual cells by determining the amount or distribution of the cellular component of interest as a function of position relative to a reference component in the individual cells.Type: ApplicationFiled: March 25, 2002Publication date: March 27, 2003Applicant: Cellomics, Inc.Inventors: Gustavo Rosania, Paul Sammak, Richard Rubin
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Publication number: 20030036853Abstract: The present invention provides methods and software for tracking individual cells during a kinetic cell screening assay.Type: ApplicationFiled: December 21, 2001Publication date: February 20, 2003Applicant: Cellomics, Inc.Inventors: Paul Sammak, Gustavo Rosania, Richard Rubin, Michel Nederlof, Oleg P. Lapets, Randall O. Shopoff, Murugan Kannan
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Publication number: 20020172964Abstract: The invention provides a method for a method for determining a best initial focal position estimate for a current sample location on a substrate comprising multiple sample locations, comprising determining the best initial focal position estimate by using a result from one or more techniques selected from the group consisting of linear regression analysis of focal positions determined for at least two other sample locations on the substrate and quadratic regression analysis of focal positions determined for at least three other sample locations on the substrate.Type: ApplicationFiled: February 1, 2002Publication date: November 21, 2002Applicant: Cellomics, Inc.Inventors: Kim Ippolito, Oleg Lapets, Michael Sipe, Randall Shopoff
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Patent number: 6365367Abstract: This invention provides environmental control devices and methods for live cell analysis. The devices of the invention combine a plate space that can hold specimen plates of a variety of sizes, gas flow control, and temperature control of the entire specimen plate.Type: GrantFiled: December 6, 1999Date of Patent: April 2, 2002Assignee: Cellomics, Inc.Inventors: Alexander L. Friedman, Stephen J. Gongaware, Albert H. Gough
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Patent number: 6103479Abstract: The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities. The present invention allows multiple types of cell interactions to be studied simultaneously by combining multicolor luminescence reading, microfluidic delivery, and environmental control of living cells in non-uniform micro-patterned arrays.Type: GrantFiled: May 29, 1997Date of Patent: August 15, 2000Assignee: Cellomics, Inc.Inventor: D. Lansing Taylor
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Patent number: 5989835Abstract: The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.Type: GrantFiled: February 27, 1997Date of Patent: November 23, 1999Assignee: Cellomics, Inc.Inventors: R. Terry Dunlay, D. Lansing Taylor