Patents Assigned to Cepheid
  • Modified cytosine polynucleotide oligomers and methods
    Patent number: 9598456
    Abstract: Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: March 21, 2017
    Assignee: Cepheid
    Inventors: Alexander A. Gall, Sergey G. Lokhov, Mikhail A. Podyminogin, Ekaterina V. Viazovkina, Kevin Patrick Lund
  • Methods of detecting sepsis
    Patent number: 9493832
    Abstract: Methods of detecting sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more microRNAs associated with sepsis are also provided. Compositions and kits are also provided.
    Type: Grant
    Filed: February 2, 2010
    Date of Patent: November 15, 2016
    Assignee: Cepheid
    Inventors: David Vilanova, David H. Persing, Olivier Delfour, Bernard Michot
  • Methods, apparatus, and computer programs for verifying the integrity of a probe
    Patent number: 9464317
    Abstract: The present invention provides methods, apparatuses and computer programs for verifying the integrity of a probe by comparing the fluorescence value of a probe to a threshold value. The invention also provides for methods, apparatuses and computer programs for normalizing the fluorescence value of a probe and detecting a target nucleic acid in a sample.
    Type: Grant
    Filed: April 26, 2007
    Date of Patent: October 11, 2016
    Assignee: CEPHEID
    Inventors: Stanley H. Sakai, William McMillan
  • Method and apparatus for storing and dispensing reagent beads
    Patent number: 9446409
    Abstract: Embodiments of the invention provide an efficient and effective technique for storing and dispensing reagent beads. In some embodiments, an apparatus is provided for dispensing reagent beads contained in a bead storage device which includes a bead carrier having a plurality of wells; a plurality of reagent beads disposed in the wells; and a cover tape releasably attached to the bead carrier to cover the wells and retain the reagent beads in the wells. The apparatus comprises a channel in which to place the bead storage device with the bead carrier facing a support wall of the channel and the cover tape facing a stripping wall of the channel. The stripping wall includes a stripping gap disposed between a stripping edge and an opposite edge, and a dispense opening provided adjacent the opposite edge on a side of the stripping wall opposite from the stripping edge.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: September 20, 2016
    Assignee: CEPHEID
    Inventors: Ronald Chang, Douglas B. Dority, Steven Montgomery
  • Multi-chambered lid apparatus
    Patent number: 9394086
    Abstract: A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container.
    Type: Grant
    Filed: March 15, 2014
    Date of Patent: July 19, 2016
    Assignee: Cepheid
    Inventor: Paul Jordan
  • Compositions comprising polynucleotides for detecting lung cancer
    Patent number: 9365903
    Abstract: Methods of detecting lung cancer, such as non-small cell lung cancer, including squamous cell carcinoma and adenocarcinoma, are provided. Methods of detecting changes in the levels of one or more small RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: June 14, 2016
    Assignee: Cepheid
    Inventors: Bernard Michot, Olivier Delfour
  • Cartridge for conducting a chemical reaction
    Patent number: 9322052
    Abstract: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: April 26, 2016
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, William McMillan, Farzad Pourahmadi, Ronald Chang, Douglas B. Dority
  • METHODS OF DETECTING LUNG CANCER
    Publication number: 20160108478
    Abstract: Methods of detecting lung cancer, such as non-small cell lung cancer, including squamous cell carcinoma and adenocarcinoma, are provided. Methods of detecting changes in the levels of one or more small RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
    Type: Application
    Filed: March 7, 2014
    Publication date: April 21, 2016
    Applicant: CEPHEID
    Inventors: Olivier Delfour, Bernard Michot
  • Apparatus for controlling and monitoring reactions
    Patent number: 9316590
    Abstract: The present invention provides a reaction vessel and apparatus for performing heat-exchanging reactions. The vessel has a chamber for holding a sample, the chamber being defined by a plurality of walls, at least two of the walls being light transmissive to provide optical windows to the chamber. The apparatus comprises at least one heating surface for contacting at least one of the plurality of walls, at least one heat source for heating the surface, and optics positioned to optically interrogate the chamber while the heating surface is in contact with at least one of the plurality of walls.
    Type: Grant
    Filed: October 1, 2012
    Date of Patent: April 19, 2016
    Assignee: Cepheid
    Inventors: Ronald Chang, Douglas Dority
  • METHODS OF DETECTING CANCER
    Publication number: 20160024586
    Abstract: Methods of detecting cancer are provided. Methods of detecting changes in the levels of one or more small RNAs associated with cancer are also provided. Compositions and kits are also provided.
    Type: Application
    Filed: March 10, 2014
    Publication date: January 28, 2016
    Applicant: Cepheid
    Inventors: Olivier Delfour, David Vilanova
  • METHODS OF DETECTING CHLAMYDIA AND GONORRHEA AND OF SCREENING FOR INFECTION/INFLAMMATION BASED ON GENOMIC COPY NUMBER
    Publication number: 20150376683
    Abstract: Compositions and methods for detecting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract.
    Type: Application
    Filed: May 22, 2013
    Publication date: December 31, 2015
    Applicant: CEPHEID
    Inventors: Alfhous Weir, David Persing, Russell Higuchi, James Wang, Reuel VanAtta, William E. Murray
  • METHODS OF DETECTING SEPSIS
    Publication number: 20150376699
    Abstract: Methods of sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more RNAs associated with sepsis are also provided. Compositions and kits are also provided.
    Type: Application
    Filed: June 1, 2015
    Publication date: December 31, 2015
    Applicant: CEPHEID
    Inventors: David Vilanova, Olivier Delfour
  • METHODS OF DETECTING LUNG CANCER
    Publication number: 20150376711
    Abstract: Methods of lung cancer in a sample from a patient are provided. Methods of detecting changes in expression of one or more target RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
    Type: Application
    Filed: May 26, 2015
    Publication date: December 31, 2015
    Applicant: CEPHEID
    Inventors: Bernard Michot, Olivier Delfour, David H. Persing
  • Fluid processing and control
    Patent number: 9212980
    Abstract: A fluid control and processing system for controlling fluid flow among a plurality of chambers comprises a body including a fluid processing region continuously coupled fluidicly with a fluid displacement region. The fluid displacement region is depressurizable to draw fluid into the fluid displacement region and pressurizable to expel fluid from the fluid displacement region. The body includes at least one external port. The fluid processing region is fluidicly coupled with the at least one external port. The fluid displacement region is fluidicly coupled with at least one external port of the body. The body is adjustable with respect to the plurality of chambers to place the at least one external port selectively in fluidic communication with the plurality of chambers.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: December 15, 2015
    Assignee: Cepheid
    Inventors: Douglas Dority, Ronald Chang
  • Methods, apparatus, and computer programs for verifying the integrity of a probe
    Patent number: 9206470
    Abstract: The present invention provides methods, apparatuses and computer programs for verifying the integrity of a probe by comparing the fluorescence value of a probe to a threshold value. The invention also provides for methods, apparatuses and computer programs for normalizing the fluorescence value of a probe and detecting a target nucleic acid in a sample.
    Type: Grant
    Filed: April 26, 2007
    Date of Patent: December 8, 2015
    Assignee: Cepheid
    Inventors: Stanley H. Sakai, William McMillan
  • TWO-PRIMER PCR FOR MICRORNA MULTIPLEX ASSAY
    Publication number: 20150322510
    Abstract: The present invention provides method for amplifying a specific RNA molecule in a sample, the method comprising: (a) adding a poly(ribonucleotide) sequence to RNA molecules in the sample; (b) reverse transcribing the poly-adenylated RNA molecules using a reverse primer comprising a sequence that anneals to said poly(ribonucleotide) sequence; and (c) amplifying and detecting the cDNA molecule(s) using the same reverse primer and using a forward primer specific for the RNA molecule to be detected; wherein at least one of the forward and reverse primers comprises a hairpin primer. The invention also provides kits useful for practicing this method.
    Type: Application
    Filed: September 25, 2013
    Publication date: November 12, 2015
    Applicant: CEPHEID
    Inventors: Russell HIGUCHI, Edwin Wei-Lung LAI, Sergey LOKHOV
  • Apparatus and method for analyzing fluid sample
    Patent number: 9156032
    Abstract: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
    Type: Grant
    Filed: April 6, 2011
    Date of Patent: October 13, 2015
    Assignee: Cepheid
    Inventors: Kurt E. Peterson, Michael T. Taylor, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Stanley H. Sakai, Jesus Ching, Douglas P. Dority, Philip Belgrader, M. Allen Northrup
  • HONEYCOMB TUBE
    Publication number: 20150275292
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
    Type: Application
    Filed: September 26, 2013
    Publication date: October 1, 2015
    Applicant: CEPHEID
    Inventors: Yuh-Min Chiang, Doug Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Apparatus and method for cell disruption
    Patent number: 9073053
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: July 7, 2015
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Phillip Belgrader, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Stanley H. Sakai, Jesus Ching
  • Molecular diagnostic system
    Patent number: D775363
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: December 27, 2016
    Assignee: CEPHEID
    Inventors: Ronald Chang, Steven M. Montgomery, Gregory E. Mote