Abstract: The present disclosure provides an N protein epitope mutation marker for preparing an epitope deletion-marked vaccine strain of type II porcine reproductive and respiratory syndrome virus (PRRSV) and use thereof, belonging to the technical field of biological products. In the mutation marker, one or more amino acids are mutated based on an epitope sequence at positions 92 to 103 of a C-terminal of an N protein of the type II PRRSV; and the epitope mutation marker has an amino acid sequence shown in SEQ ID NO: 1, where X1 is selected from the group consisting of T, P, and A; and X2 is selected from the group consisting of V and A.

Abstract: The present disclosure provides a preparation method of resveratrol nervonic acid ester, comprising the following steps of: step 1: weighing resveratrol, a nervonic acid and thiocarbonyldiimidazole according to a molar ratio of 1:1:0.8 to 1:7:11.2 in a reactor; step 2: adding a certain amount of solvent into the reactor to completely dissolve the reactants; and step 3: stirring for reaction at room temperature for 30 min to 180 min. The preparation method is safe, efficient and clean, and enables synthesis of the resveratrol nervonic acid ester with high conversion rate, high purity and high yield.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate.

Abstract: The present disclosure belongs to the technical field of biological products for veterinary medicine, and specifically relates to a recombinant foot-and-mouth disease virus (FMDV) with a reduced immunosuppressive activity, a preparation method and use thereof, and a recombinant vaccine strain. According to the present disclosure, it is firstly discovered that FMDV 3B protein has an immunosuppressive function, and key sites for exerting the immunosuppressive function are found. A recombinant FMDV vaccine strain with a lost immunosuppressive function in FMDV 3B protein is constructed by introducing amino acid mutations into three repeated copies of FMDV 3B protein.

Abstract: A multilayered rail guide vehicle system for use in three-dimensional planting in a plant factory and a control method therefor, the system includes an upper computer, cultivation shelves, traveling devices, lifting devices, and an electric control system. The traveling devices and the lifting devices are arranged in a body of a rail guide vehicle. The upper computer is in wireless communication with the electric control system. A nutrient solution tank is arranged on each of the cultivation shelves, and a plurality of cultivation positions are arranged on the nutrient solution tank. The electric control system is arranged in the body of the rail guide vehicle, so as to obtain an operation mode and target information after receiving a task instruction transmitted by the upper computer, select and switch a positioning mode, and drive the traveling devices and the lifting devices to perform traveling and lifting positioning according to operation procedures.

Abstract: A soil conditioner spreader and an operation method thereof are provided. The soil conditioner spreader includes a spreader body, a ridger, a solid tank, two spiral drills, a rotary tillage wheel and a mulching device, where the spreader body is provided with a hopper in a front part. The hopper stores a conditioner. The solid tank is arranged above the spiral drills. The spreader body is connected to the solid tank through a feeding track. The ridger, the spiral rills, the rotary tillage wheel and the mulching device are successively arranged at the bottom of the spreader body from the rear to the front. The ridger ridges first, and then the spiral drills perform deep plowing, rotary tillage and scarification and apply the conditioner to a ridge. The rotary tillage wheel performs rotary tillage, and a nozzle sprays a nutrient solution or an herbicide/insecticide. Finally, the mulching device mulches.

Abstract: An evaluation method of mixing uniformity of composite powder includes: determining raw materials of composite powder to be evaluated and mass ratio; mixing to obtain multiple standard composite powder with different mixing time; determining flow energy of each standard composite powder; analyzing the flow energy of multiple standard composite powders by significant difference method, determining at least 3 consecutive standard composite powders with no significant difference in flow energy according to mixing time from small to large, defining as uniform-mixed standard composite powder, calculating average value of flow energy of uniform-mixed standard composite powder, and recording as standard flow energy TFEs; determining the flow energy of composite powder to be evaluated, calculating percentage difference P·Vds between TFEd and TFEs, and evaluating mixing uniformity of composite powder according to P·Vds.

Abstract: The present disclosure belongs to the technical field of African swine fever (ASF) treatment, in particular to use of a compound aprepitant in prevention or treatment of the ASF. In the present disclosure, it is found that the compound aprepitant can significantly inhibit replication of an African swine fever virus (ASFV); in addition, it is found that the aprepitant inhibits transcription and protein expression of D1133L, and reduces transcription and protein expression levels of p30 and p72, preventing virus invasion on host cells. Therefore, the compound aprepitant can be used to inhibit early and late infection of the ASFV. Accordingly, the compound aprepitant can be used to prevent or treat the ASF.

Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

Abstract: The present disclosure provides a method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, and belongs to the technical field of microorganisms. The method includes: mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid, and subjecting a resulting mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella. The method provided by the present disclosure can successfully knock out the NMT gene from Eimeria tenella, which lays a foundation for studying the function of the Eimeria tenella gene and developing a vaccine therefor.

Abstract: The present invention relates to a margarine substitute loaded with trans-resveratrol/glycoside and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a peanut protein dispersion, adding a transglutaminase thereto, performing a cross-linking reaction under a water bath condition, and heating up to obtain a monolithic gel; continuing to add water to the monolithic gel, shearing the resultant at a high speed, homogenizing at a high pressure, performing ultrasonication, and adjusting pH to obtain a microgel particle dispersion; (2) adding a trans-resveratrol/glycoside to an edible oil in the dark, stirring well, continuing to add the microgel particle dispersion obtained in step (1), and shearing the resultant at a high speed to obtain a high internal-phase Pickering emulsion, i.e. a margarine substitute loaded with trans-resveratrol/glycoside.

Abstract: The invention discloses a method of rapid generation-adding breeding of rice in the technical field of rice breeding, including soaking seeds of the rice, germination, seedlings cultivation, managing and regulating of the rice at the growth stage, and harvesting the rice. The managing and regulating the rice at the growth stage includes dynamic light quality and photoperiod control in vegetative growth period, heading period and pustulation period; growth period involves light environment regulation with the ratio of red light:blue light:white light of 0.8-1:0.8-1:1.0, photoperiod of 16-18 h; heading period involves light environment regulation with the ratio of red light:blue light:white light of 1-2:0.5-1:1, photoperiod of 12-13.5 h; and pustulation period includes light environment regulation with the ratio of red light:blue light:white light of 1-2:1:1, photoperiod of 16-18 h.

Abstract: The present disclosure relates to a rice PAL1 gene, an encoding protein and use thereof. The rice PAL1 gene has the nucleotide sequence shown in SEQ ID NO. 1, and the rice PAL1 protein has the amino acid sequence shown in SEQ ID NO. 4. Mutation of the gene leads to reduction of rice plant height and panicle length, while decreasing the number of primary branches, secondary branches and grains per panicle. It is found that the PAL1 gene can restore a mutant panicle type to a normal phenotype. The present disclosure provides a PAL1 gene functioning as a regulator of the rice panicle length and an encoding protein thereof. A rice panicle type is an important trait influencing rice yield. Therefore, it will be desired to directionally design a plant type and improve the rice yield by regulating panicle traits of rice with the PAL1 gene.

Abstract: The present invention provides an antimicrobial peptide ID13 (with a sequence set forth in SEQ ID NO. 1), a preparation method and use thereof. The antimicrobial peptide ID3 is successfully expressed in Pichia pastoris using genetic engineering technology. The antimicrobial peptide ID13 of the present invention has an excellent bactericidal activity against Gram-positive bacteria such as Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus suis, and has a low hemolytic activity against mouse red blood cells and a low murine macrophage cytotoxicity.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Abstract: The present disclosure provides a subunit vaccine of contagious caprine pleuropneumonia and preparation method and use thereof, belonging to the technical field of preparation of animal infectious disease vaccine. The combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins comprises Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E; the mass ratio of Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E is (0.5-1.5):(0.5-1.5):(0.5-1.5):(0.5-1.5):(0.5-1.5). The subunit vaccine of contagious caprine pleuropneumonia comprises the combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins and adjuvant; the subunit vaccine has the advantages of high safety, good immunization effect, high stability and minor adverse effects.

Abstract: The present disclosure discloses an intelligent point-of-care testing device for mycotoxins based on a quantum dots test strip. A first light source and a second light source are used for exciting a test strip which is placed in a shell and coated with a mycotoxin antigen to obtain emitted light, and a light filtering component is used for filtering out impurity light. A clamping mechanism is used for clamping and fixing an image capturing component. The image capturing component is used for capturing, an image of mycotoxins based on a quantum dots test strip. A processor is used for analyzing and calculating image information to obtain a mycotoxin content result. The testing time is shortened, and the portability and practicability of mycotoxin testing are improved. Quantitative instant testing of single or multi-toxin mixed pollution is achieved, and the technical problem of fluorescence quantification is solved.

Abstract: Disclosed is a method for regulating a porous structure and texture property of a freeze-dried pectin aerogel, which comprises the following steps of step 1: preparation of pectin/starch solution by mixing pectin powder and a gelatinized starch solution to form a stable pectin/starch mixed solution, a concentration of the pectin being 0.05% to 0.2% (w/v); step 2: induction of gel reaction by adding D-(+)-Glucono-?-lactone and calcium carbonate into the stable pectin-starch mixed solution; and step 3: vacuum freeze of the starch/pectin composite gel subjected to the gel reaction to obtain the freeze-dried pectin/starch aerogel with an adjustable porous structure and an improved texture characteristic. Controllable regulation of the porous structure of the freeze-dried pectin aerogel is achieved by the method without complex pretreatment. Raw material ingredients applied in the method are cheap, easy-to-access, and has low-toxicity.

Abstract: The present invention provides a soybean anti-pod-shattering major QTLqPD05, and a mapping method and application thereof, and belongs to the field of QTL mapping. The soybean anti-pod-shattering major QTL is mapped on the chromosome 5 of soybean at a physical position between 40448596-40703417. For the method for mapping the soybean anti-pod-shattering major QTL, a SLAF marker is screened at the whole genome level of the soybean by utilizing a SLAF-seq sequencing technology, so as to explore the QTLs related to pod shattering from this population. By using a material of a RIL7 population which has pod-shattering soybean and anti-pod-shattering soybean as the parents, a high-density genetic linkage map covering the whole genome of soybean is constructed, and QTL mapping of the anti-pod-shattering trait is carried out on this population to obtain QTLs related to anti-pod-shattering.

Abstract: A continuous differential-pressure steam sterilization system for a powder, and belongs to the field of material sterilization includes: a superheated steam generation system, a steam pressure and flow rate control system, a quantitative feeding system, an instantaneous differential-pressure sterilization system, a dust explosion suppression system, a sterile cooling system, a primary gas-solid separation system, a secondary gas-solid separation system, a sterile storage system, a steam recovery and reheating system, and a condensate recovery system. The continuous differential-pressure steam sterilization system shortens the thermal contact time and mainly accumulates the heat on the surface of the powder, rather than in the center of the powder, which reduces the damage to the nutritional quality of the powder.