Abstract: A key DNA sequence for regulating a maize leaf angle and a mutant thereof are provided, which have polynucleotide sequences shown in SEQ ID No. 1 and SEQ ID No. 2, respectively. The DNA sequence for regulating a maize leaf angle and the mutant thereof provided by present disclosure can regulate the expression of ZmNAC16 gene in a maize pulvinus, and thus can be used for the improvement of maize leaf angle and plant type and further for the cultivation of new maize varieties. The present disclosure further provides specific detection primers for detecting mutations of the DNA key sequence and the mutant, and detection primers for detecting an expression level of ZmNAC16 gene in maize. These detection primers can be used to directionally improve a maize leaf angle and also shows application potential for breeding of dense-planting-tolerant and high-yield maize.

Abstract: A portable pesticide application equipment with accurate application, which includes a pesticide tank. Rollers are arranged at the bottom of the pesticide tank. An inclined pesticide guide plate is arranged below the filter. A side connector is arranged at one side of the pesticide tank. The side connector is connected with a long hose. The long hose is connected with a pesticide discharging device. The pesticide discharging device includes a pesticide control box, two sides of which are hinged with a first iron handle and a second iron handle. A first nozzle is connected with the bottom end of the first iron handle while a second nozzle is connected with the bottom end of the second iron handle. The upper end of the first iron handle is inserted into a first support rod while the upper end of the second iron handle is inserted into a second support rod.

Abstract: The present invention provides a method for improving transformation efficiency of a plant. The method according to the present invention comprises the use of a nucleic acid encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4, or a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 2 or 4, and having a function of improving transformation efficiency of a plant.

Abstract: The invention discloses a method of rapid generation-adding breeding of rice in the technical field of rice breeding, including soaking seeds of the rice, germination, seedlings cultivation, managing and regulating of the rice at the growth stage, and harvesting the rice. The managing and regulating the rice at the growth stage includes dynamic light quality and photoperiod control in vegetative growth period, heading period and pustulation period; growth period involves light environment regulation with the ratio of red light:blue light:white light of 0.8-1:0.8-1:1.0, photoperiod of 16-18 h; heading period involves light environment regulation with the ratio of red light:blue light:white light of 1-2:0.5-1:1, photoperiod of 12-13.5 h; and pustulation period includes light environment regulation with the ratio of red light:blue light:white light of 1-2:1:1, photoperiod of 16-18 h.

Abstract: The present invention discloses a credible fruit traceability method and device based on fruit texture atlas and blockchain. According to the present invention, images of fruit pedicel part and fruit navel part of a single fruit are obtained, and the images are grayed and normalized to be converted into rectangular images; features are extracted from the rectangular images, respectively, and are encoded to obtain a fruit pedicle feature code table and a fruit navel feature code table; the two feature code tables are subjected to a merging operation to obtain a combined bidirectional feature code table, thus forming a unique fruit texture atlas. By processing the fruit texture atlas, the fruit texture atlas information and related information are stored on the blockchain.

Abstract: The present invention relates to an early warning method before the occurrence of aflatoxin contamination.

Abstract: The present invention relates to an aflatoxin contamination risk warning molecule and use thereof. The steps are as follows: weighing a quantitative sample, extracting the aflatoxin contamination risk warning molecule to obtain a sample extract, and detecting and analyzing the sample extract to obtain a quantitative result of the aflatoxin contamination risk warning molecule; performing modeling with a chemometrics method using the content of one or more of the aflatoxin contamination risk warning molecules as a variable to obtain a classification prediction model, and performing risk assessment on aflatoxin contamination risk of the sample based on the classification prediction model, wherein a warning molecule of an aflatoxin toxigenic strain is one or a combination of more than one of versiconol (VOH), versicolorin B (Ver B), and 5-methoxysterigmatocystin (5-MST).

Abstract: The present application relates a composite multi-epitope expression cassette, a recombinant virus composed thereof and application thereof, and in particular to a chimeric recombinant Newcastle disease virus inserted with an IBV epitope cassette and a vaccine prepared by using the virus. The expression cassette comprises: (a) T cell epitopes derived from S1 proteins of avian infectious bronchitis virus Holte strain and avian infectious bronchitis virus QX-like strain; and (b) B cell epitopes derived from S1 protein of avian infectious bronchitis virus Australian T strain. In the present application, the multi-epitope chimeric ST/B gene of avian infectious bronchitis virus is inserted into the backbone of LaSota strain, so that the LaSota strain can express S1-T/B protein. Thus, the purpose of preventing both ND and IB diseases is achieved. In addition, the T cell epitopes and B cell epitopes act synergistically to produce an earlier and more comprehensive immune response against virus.

Abstract: A green method and application for inhibiting toxin-producing Aspergillus flavus are provided. After a ?-Fe2O3 nanorod nanomaterial is brought into contact with spores of toxin-producing Aspergillus flavus without germination or after germination for irradiation, under irradiation of a light source, inhibiting growth of the toxin-producing Aspergillus flavus to lower a content of aflatoxin. In the disclosure, the growth of hyphae and the germination of spores of the Aspergillus flavus may be effectively inhibited, the growth of the toxin-producing Aspergillus flavus may be inhibited, the pollution of agricultural products such as peanuts by the Aspergillus flavus may be effectively prevented, and the content of aflatoxin may be reduced, so that the disclosure has broad application prospects.

Abstract: The present application provides an application of quercetin in a plant senescence accelerator, which belongs to the field of plant senescence accelerators and its application, and can prepare a plant senescence accelerator by using quercetin and apply the accelerator in practical production to accelerate the senescence process of plant particularly crop leaves. The quercetin plant senescence accelerator is prepared by mixing a quercetin mother solution with a 1/2 MS solution of 2-(N-morpholino)ethanesulfonic. In the present application, by adopting directly spraying method, the senescence process of plant leaves can be accelerated, and will not cause additional adverse reactions to plants.

Abstract: The present invention relates to the technical field of bee product processing, and discloses a continuous drying apparatus for bee pollen and related drying method. The continuous drying apparatus for bee pollen comprises: a box body; a first drying conveying structure; a second drying conveying structure; and a heat pump dehumidification heating structure. The continuous drying apparatus for bee pollen has the advantage of high drying efficiency.

Abstract: The disclosure provides a recombinant nucleic acid of Seneca valley virus, a recombinant vaccine strain and preparation method and use thereof, and relates to the technical field of genetic engineering. The disclosure provides the recombinant nucleic acid of Seneca valley virus, recombinant Seneca valley virus comprising the recombinant nucleic acid, recombinant Seneca valley virus encoded by the recombinant nucleic acid, recombinant Seneca valley virus vaccine strain comprising the recombinant Seneca valley virus and preparation method and use thereof. According to the disclosure, a vaccine strain characterized by high antigen production capacity, remarkably reduced pathogenicity (even having no pathogenicity to pigs), strong antibody induction activity, high immune protection rate is prepared. The vaccine strain remarkably improves the biological safety and can be used for preventing and controlling Seneca valley virus in China and the neighboring countries.

Abstract: The present invention relates to an organic nutrient-based dissolution accelerator for potassium sulfate and a preparation method therefor and the use thereof. The organic nutrient-based dissolution accelerator for potassium sulfate of the present invention consists of an organic acid mixture solution, tyrosine, leucine, threonine, alcohol amine, and the like. The organic nutrient-based dissolution accelerator for potassium sulfate of the present invention can not only substantially improve the dissolution speed of potassium sulfate by 6-120 times, but also provides organic nutrition for crops to achieve a synergy with inorganic nutrition to further improve the nutritional efficiency of potassium and sulfur elements.

Abstract: The present invention relates to a method for preparing a humic acid-containing synergistic carrier for accelerating urea conversion. The preparation method includes steps of, for example, preparing an extractant for humic acid, preparing a humic acid extract and preparing a humic acid-containing synergistic carrier. Combination of the humic acid-containing synergistic carrier with urea can accelerate urea conversion, and the residual urea rate will be reduced by 22.7%-29.1% than a commercially available urea. The present invention provides a new idea for improving the utilization rate of nitrogen fertilizer in soil with low urease activity. Compared with a commercially available urea, the urea containing the humic acid-containing synergistic carrier of the present invention can increase the rice yield and the utilization rate of nitrogen fertilizer by 9.7% and 8.3%, respectively.

Abstract: This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Methods to create or alter pesticidal proteins are provided for altered or enhanced pesticidal activity.

Abstract: The present invention discloses a preparation method of instant bone milk, including: washing edible animal bones and then crushing, adding a certain amount composite effect enhancer, performing two high pressure hydrolysis-instantaneous pressure relief in high pressure hydrolysis chamber, collecting liquid-phase materials, performing centrifugation for degreasing, wet sieving, homogenizing, spray drying to obtain powdered instant bone milk. A device for preparation method of instant bone milk comprises a high-pressure hydrolysis tank, a grading conveying pipe and a gas-liquid separator. The instant bone milk prepared by the present invention has stable chemical structure, uniform particle size distribution and regular normal distribution, high protein solubility and good solution stability. And the preparation method has simple process, low cost equipment and good versatility.

Abstract: The invention discloses a liquefaction device of hard bone, comprising: outer cavity having upper end detachably sealed with upper cover, and lower end openable/closable sealed with lower sealing cover, first liquefaction cavity coaxially slidably disposed in outer cavity, stainless steel cage disposed coaxially in first liquefaction cavity, second liquefaction cavity fixed to outer sidewall of outer cavity such that lower end portion of outer cavity is located in second liquefaction cavity. The invention also provides a method for co-production of bone collagen polypeptide and ultrafine bone powder based on liquefaction device, including: selecting hard bones, crushing; performing first-stage and second-stage liquefaction to obtain liquid phase and solid phase; centrifuging, concentrating, drying liquid phase to obtain bone collagen polypeptide; drying, coarsely and superfine pulverizing solid phase to obtain ultrafine bone powder.

Abstract: The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.

Abstract: A method for identifying and evaluating toxigenic capability of an aflatoxigenic strain. A ratio of the aflatoxin yield to Nor-1 gene transcriptional quantity is determined to have high relative stability. An Aspergillus flavus strain toxigenic capability identification model is established, and thus a regression equation between the Aspergillus flavus toxigenic capability and the ratio AFT/Nor-1 of the aflatoxin yield to the Nor-1 gene transcriptional quantity is obtained.

Abstract: The present invention discloses genes and SNP markers significantly associated with lint percentage trait in cotton, and use thereof. The genes significantly associated with the lint percentage trait in cotton are genes Gh_D05G1124, Gh_D05G0313, and GhWAKL3. In the present invention, a CottonSNP63K gene array is used for genotyping, and genome re-sequencing data are analyzed to identify SNP markers significantly associated with the lint percentage trait in cotton. Moreover, the present invention also discloses use of the genes and SNP markers, which are significantly associated with the lint percentage trait in cotton, in cotton germplasm identification, breeding, or genetic diversity analysis.