Abstract: Radical cascade reactions enabling sequence-controlled ring-closing polymerization and ring-opening polymerization for the controlled synthesis of polymers with complex main-chain structures are provided. Facile syntheses leading to low-strain macrocyclic monomers consisting of the ring-opening triggers and extended main-chain structures are also provided. The present disclosure further provides methods for excellent control over polymer molecular weights and molecular weight distributions and high chain-end fidelity allows for the preparation of polymeric systems with well-defined architectures. Further provided are the general nature of the radical cascade-triggered transformations in polymer chemistry, and its application to the synthesis of polymers with diverse main-chain structural motifs with tailored functions. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
Abstract: An immunogenic fusion protein for use as a mucosal vaccine is provided, which includes: i) one or more FcyR1-binding domains; ii) one or more antigens from one or more infectious disease organisms; and iii) one or more FcRn-binding domains.
Type:
Grant
Filed:
February 11, 2015
Date of Patent:
November 30, 2021
Assignees:
Albany Medical College, University of Washington through its Center for Commercialization
Abstract: The invention provides compounds of the formula (I): wherein R1 and R2 are defined in the specification, to pharmaceutical compositions comprising the compounds and the compounds for use as medicaments. The compounds potentiate AMPA receptor function and are expected to be useful in the treatment of central nervous system disorders, for example in the treatment of depressive disorders, mood disorders and cognitive dysfunction associated with neuropsychiatric disorders such as schizophrenia.
Type:
Grant
Filed:
February 9, 2018
Date of Patent:
November 30, 2021
Assignee:
University College Cardiff Consultants Limited
Inventors:
Simon Ward, Paul Beswick, Lewis Pennicott, Tristan Reuillon
Abstract: The present disclosure describes a strategy to create self-healing, slippery self-lubricating polymers. Lubricating liquids with affinities to polymers can be utilized to get absorbed within the polymer and form a lubricant layer (of the lubricating liquid) on the polymer. The lubricant layer can repel a wide range of materials, including simple and complex fluids (water, hydrocarbons, crude oil and bodily fluids), restore liquid-repellency after physical damage, and resist ice, microorganisms and insects adhesion. Some exemplary applications where self-lubricating polymers will be useful include energy-efficient, friction-reduction fluid handling and transportation, medical devices, anti-icing, optical sensing, and as self-cleaning, and anti-fouling materials operating in extreme environments.
Type:
Grant
Filed:
May 7, 2018
Date of Patent:
November 30, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
Joanna Aizenberg, Michael Aizenberg, Jiaxi Cui, Stuart Dunn, Benjamin Hatton, Caitlin Howell, Philseok Kim, Tak Sing Wong, Xi Yao
Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
Type:
Application
Filed:
March 15, 2021
Publication date:
November 25, 2021
Applicants:
The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
Abstract: A mosaic-style design aerogel window system having two panes of translucent material assembled parallel to each other in a frame to form a window panel is disclosed. A variety of aerogel monoliths of various colors are assembled in a layer between the two panes of translucent material such that edges of adjacent aerogel monoliths mate with each other. Aerogel monoliths are prepared from a plurality of cut or molded shapes of aerogel monoliths, each having at least one dimension of ¼ inch or greater. At least some of the plurality of aerogel monoliths have dyes or salts incorporated into a precursor recipe to impart color to the colored aerogel monoliths.
Type:
Application
Filed:
May 19, 2021
Publication date:
November 25, 2021
Applicant:
Union College
Inventors:
Zineb Hajjaj, Ann M. Anderson, Mary K. Mahony
Abstract: Disclosed are methods of genomic modification of skeletal and cardiac muscle using sequence-targeting nucleases and a donor sequence delivered via a virus.
Type:
Application
Filed:
May 3, 2019
Publication date:
November 25, 2021
Applicant:
President and Fellows of Harvard College
Abstract: A method for sequentially encrypting and decrypting doubly linked lists based on double key stream ciphers comprises: establishing a plaintext set M according to a plaintext file; using the plaintext set M as an initial value and performing iterative decryption to obtain a ciphertext set C, wherein a key set P and an algorithm set A are used during the iterative decryption; for the ciphertext set C, performing multiple decryptions by calling the key set P and keys in the key set P, wherein a key set P and an algorithm set A are used during the decryptions; and, converting the obtained result of decryption into a plaintext file.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
November 23, 2021
Assignees:
Zhuhai College of Jilin University, Jilin University
Abstract: The present invention provides methods for analyzing polynucleotides such as genomic DNA. In some embodiments, the disclosure provides a method for preparing and amplifying a genomic DNA library in situ in a fixed biological sample. The method comprises treating a fixed biological sample with an insertional enzyme complex to produce tagged fragments of genomic DNA. The method further comprises circularizing the tagged fragments of genomic DNA. The method further comprises amplifying the tagged fragments of genomic DNA.
Type:
Grant
Filed:
July 24, 2018
Date of Patent:
November 23, 2021
Assignees:
Massachusetts Institute of Technology, President and Fellows of Harvard College
Inventors:
Fei Chen, Andrew C. Payne, Jason D. Buenrostro, Paul Reginato, Edward Stuart Boyden, Shahar Alon
Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA or RNA-targeting systems comprising a novel DNA or RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
Type:
Grant
Filed:
December 14, 2017
Date of Patent:
November 23, 2021
Assignees:
The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College, Rutgers, the State University of New Jersey, The United States of America, as represented by the Secretary, Department of Health and Human Services, Skolkovo Institute of Science and Technology
Inventors:
Eugene Koonin, Feng Zhang, Yuri I. Wolf, Sergey Shmakov, Konstantin Severinov, Ekaterina Semenova, Leonid Minakhin, Kira S. Makarova, Silvana Konermann, Julia Joung, Jonathan S. Gootenberg, Omar O. Abudayyeh
Abstract: Provided herein are palladium complexes comprising a ligand of Formula (A?) and a ligand of Formula (B), wherein R1-R18 are as defined herein. The palladium complexes are useful in methods of fluorinating aryl and heteroaryl substrates. Further provided are compositions and kits comprising the palladium complexes.
Type:
Grant
Filed:
March 9, 2017
Date of Patent:
November 23, 2021
Assignees:
President and Fellows of Harvard College, Studiengesellschaft Kohle mbH
Abstract: Most automatic cuff blood pressure (BP) measurement devices are based on oscillometry. These devices estimate BP from the envelopes of the cuff pressure oscillations using fixed ratios. The values of the fixed ratios represent population averages, so the devices may be accurate only in subjects with normal BP levels. A patient-specific oscillometric BP measurement method was developed. The idea was to represent the cuff pressure oscillation envelopes with a physiologic model and then estimate the patient-specific parameters of the model, which includes BP levels, by optimally fitting it to the envelopes.
Type:
Grant
Filed:
September 9, 2016
Date of Patent:
November 23, 2021
Assignees:
Board of Trustees of Michigan State University, University of Maryland, College Park
Abstract: The present invention generally relates to microfluidics and/or epigenetic sequencing. In one set of embodiments, cells contained within a plurality of microfluidic droplets are lysed and the DNA (e.g., from nucleosomes) within the droplets are labeled, e.g., with adapters containing an identification sequence. The adapters may also contain other sequences, e.g., restriction sites, primer sites, etc., to assist with later analysis. After labeling with adapters, the DNA from the different cells may be combined and analyzed, e.g., to determine epigenetic information about the cells. For example, the DNA may be separated on the basis of certain modifications (e.g., methylation), and the DNA from the separated nucleosomes may be sequenced using techniques such as chromatin immunoprecipitation (“CUP”). In some cases, the DNA sequences may also be aligned with genomes, e.g., to determine which portions of the genome were epigenetically modified, e.g., via methylation.
Type:
Application
Filed:
May 25, 2021
Publication date:
November 18, 2021
Applicants:
President and Fellows of Harvard College, The General Hospital Corporation
Inventors:
David Weitz, Assaf Rotem, Oren Ram, Bradley E. Bernstein
Abstract: The present disclosure provides a newly conceived of platform for identifying novel pain and/or pruritus-inhibiting agents (e.g., small molecule compounds, peptides, antigen binding proteins (e.g., antibodies or antibody fragments) that are efficacious, safe, and non-addictive alternatives for pain and pruritus management in place of (or in some embodiments, in combination with) “first line” treatments, such as gabapentin, pregabalin, and opioids. The present disclosure also provides for a method of treating pain and/or a pruritus comprising administering a therapeutically effective amount of an agent that activates a Gai/o-coupled G-Protein Coupled Receptor (GPCR) that is selectively expressed in the nociceptor and/or pruriceptor neuron subtypes of the somatosensory neurons.
Type:
Application
Filed:
May 17, 2021
Publication date:
November 18, 2021
Applicant:
President and Fellows of Harvard College
Abstract: A device and method are disclosed for intravenous cannulation. The device employs a spring to precisely and accurately control the force and velocity imparted to a piercing needle, with mechanical constraints built into the device to precisely and accurately control the distance through which the piercing needle travels. A sheathed-needle configuration is used with an outer, echogenic needle that can be seen via ultrasound; and an inner needle used to pierce the vein. The operator releases the piercing needle with a controlled velocity, controlled force, and controlled travel distance. The device reduces the human error and complications that have previously been associated with manually piercing a vein for cannulation.
Type:
Application
Filed:
October 14, 2019
Publication date:
November 18, 2021
Applicant:
Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
Inventors:
Jacob Bursavich, Benjamin Seago, Logan Daigle
Abstract: The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant.
Abstract: Exemplary embodiments provide systems, devices and methods for the fabrication of three-dimensional polymeric fibers having micron, submicron and nanometer dimensions, as well as methods of use of the polymeric fibers.
Type:
Grant
Filed:
November 11, 2019
Date of Patent:
November 16, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
Kevin Kit Parker, Grant Michael Gonzalez, Holly M. Golecki, Kwanwoo Shin, Josue A. Goss
Abstract: The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
Type:
Grant
Filed:
March 15, 2018
Date of Patent:
November 16, 2021
Assignees:
The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
Inventors:
Omar Abudayyeh, James Joseph Collins, Jonathan Gootenberg, Feng Zhang, Eric S. Lander, Aviv Regev
Abstract: A stretchable electrooptical device includes a liquid crystal cell disposed between first and second ionic conducting gel layers; and first and second electronic conductors in electrical contact with the first and second ionic conducting gel layers, respectively, said first and second electronic conductors connectable to an external voltage source.
Type:
Grant
Filed:
March 7, 2018
Date of Patent:
November 16, 2021
Assignee:
President and Fellows of Harvard College