Abstract: A lectin-containing, water-insoluble gel matrix with a glycoprotein reversibly bound thereto can be used for fractionating a polysaccharidic substance capable of being separated into components of differing activity by virtue of differing affinity for the glycoprotein.

Abstract: Novel antihemophilic factor concentrates and process for making the same are disclosed. An aqueous solution of antihemophilic factor proteins is obtained, preferably by solubilizing antihemophilic factor proteins from blood plasma cryoprecipitate in water. The aqueous solution is purified to remove unwanted protein, for example, by mixing the aqueous solution with aluminum hydroxide. Then, the aqueous solution is ultrafiltered to concentrate it, mixed with buffer and saline and adjusted to an acid pH, and freeze-dried.

Abstract: An improved method for testing the integrity of containers and closures for same is disclosed which is simple, easy to perform, and possesses a high degree of sensitivity. The method comprises immersing a container (whose walls are substantially transparent and in which a substantially transparent liquid is stored) into a solution of a dye under conditions which will favor the passage of the dye through any leaks present in the container or closure and into the liquid. The container is then placed in the path of a laser beam emitting a wavelength which approximates the fluorescent excitation frequency of the dye. If the color of the beam transmitted through the liquid is that which is characteristic for the fluorescing dye, a leak in the system is indicated.

Abstract: A method is disclosed for separating alpha-1-proteinase inhibitor from a blood plasma fraction particularly from the Cohn fractionation scheme. An aqueous solution of the blood plasma fraction is held at a pH of about 6.5-8.5, and a temperature of about 2.degree.-50.degree. C. for a period of about 0.2-24 hours and then mixed with a poly-condensed polyglycol in a proportion of about 10-15 parts of polymer per 100 ml of aqueous solution containing the blood plasma fraction. The mixture may be held at a temperature of about 2.degree.-10.degree. C. for a period of about 1-24 hours. Next, the pH of the mixture is adjusted to about 4.6-5.7, to selectively precipitate unwanted proteins from the solution of alpha-1-proteinase inhibitor which is separated from the solution and purified further.

Abstract: Air bubbles in a transparent intravenous solution conduit are detected, regardless of whether the solution is opaque or clear, by directing a light source at the conduit and detecting light shining through the solution with a first light sensor while detecting light reflected from the interior wall of the conduit with a second light sensor. If liquid is present in the conduit, whether clear or opaque, one sensor will see a low light level while the other sees a high level. If air is present, both sensors see a high light level. By EXCLUSIVE-OR'ing the outputs of the two sensors, a logic signal is obtained which is high only when the presence of liquid in the conduit is being detected. Means using two sensor assemblies spaced axially along the conduit, and a counter associated with each, are also provided to allow passage of minor air bubbles, and to provide a backup signal representative of a predetermined volume of air passing either assembly independently of the other.

Abstract: Blood-coagulation-promoting products substantially free of thrombin are prepared from human blood plasma by contacting a human blood plasma fraction containing coagulation Factors II, VII, IX and X with an anion exchanger to adsorb the coagulation Factors, which are subsequently eluted from the anion exchanger. The eluate is treated to generate a substance having Factor VIII Inhibitor Bypassing Activity and being substantially free of thrombin, and activated Factor X.

Abstract: The disclosure pertains to a concentrate for controlling bleeding in hemophilia, free of thrombin, heparin, thromboplastin activity, anticomplement activity, depressor activity, and activated Factor X, and containing coagulation Factors II, VII, IX, and X in non-activated form, having a specific activity of at least about 1.5 Factor IX units per milligram of total protein and a Factor IX: Factor VII ratio of at least about 6:1. In the method of the invention a blood fraction containing coagulation Factors II, VII, IX, and X is applied to an anion exchanger to adsorb the coagulation Factors, which are selectively eluted therefrom using an aqueous sodium chloride-citrate solution of increasing ionic strength. The eluate is treated to reduce its concentration of non-volatile salt and concentrated to yield the above product.

Abstract: A means for releasable sealing a flexible conduit is disclosed, having particular use in a conduit connecting multiple blood bags. The conduit contains within a portion of itself a flexible strip or tubular insert. This portion of the conduit is positively closed by folding it back on itself and the closed portion is maintained by a clamping or restraining member. Upon release of the clamping member following a heat sterilization procedure, the conduit is readily opened for fluid flow therethrough. The conduit, which without the insert would normally be fused together as a consequence of the heat sterilization, is open by virtue of the insert being made of a material which does not fuse together under these conditions.

Abstract: An improvement in a flow control device for permitting fine adjustment of flow rates of fluid in tubing and maintaining established rates of flow. The device includes a clamping member pivotally mounted adjacent a clamping surface and a tubing-engaging surface on the clamping member which progressively closes the orifice of tubing as the clamping member is rotated in one direction. Coacting means on the clamping member cooperate with actuating means on a force-inducing member mounted for rotational movement on the body of the device. The coacting means is located at a point from the point of pivotal attachment of the clamping member which is at least twice the distance from the pivotal attachment to the tubing-engaging surface. Thus a large movement at the coacting point translates into a small movement of the tubing-engaging surface and results in fine adjustment of flow rates.

Abstract: Methods are disclosed for obtaining fibronectin and antihemophilic factor in commercial quantities from blood plasma. In the disclosed methods a blood plasma fraction containing fibronectin and antihemophilic factor is isolated from blood plasma and then solubilized in an aqueous medium. The solution is acidified and chilled at a temperature sufficient to form an acid-chill precipitate containing a major proportion of the fibronectin and a solution containing a major proportion of the antihemophilic factor of the blood plasma fraction. Fibronectin and antihemophilic factor are then isolated respectively from the precipitate and solution.

Abstract: A unit for rapid priming and flow of liquid through the unit is disclosed. The unit comprises a connector for communicating with liquid in a sealed container, the connector having an exit orifice around which is secured one end of a primer tube. The other end of the primer tube is joined to a pooling conduit. The diameter of the primer tube is greater than the diameter of a drop of liquid released from the orifice and the diameter of the pooling conduit is no greater and preferably slightly less than the diameter of the drop of liquid. Multiple units can be joined to a common tube of enlarged diameter which leads into a pooling and dispensing container.

Abstract: An improvement in a flow control device for permitting fine adjustment of flow rates of fluid in tubing and maintaining established rates of flow. The device includes a clamping member pivotally mounted adjacent a clamping surface and a tubing-engaging surface on the clamping member which progressively closes the orifice of tubing as the clamping member is rotated in one direction. Coacting means on the clamping member cooperate with actuating means on a force-inducing member mounted for rotational movement on the body of the device. The coacting means is located at a point from the point of pivotal attachment of the clamping member which is at least twice the distance from the pivotal attachment to the tubing-engaging surface. Thus a large movement at the coacting point translates into a small movement of the tubing-engaging surface and results in fine adjustment of flow rates.

Abstract: Influenza antibody levels in serum can be measured via plaque reduction neutralization (PRN) technique. Test is more sensitive and specific for measuring anti-infective influenza antibodies than present hemagglutination inhibition (HAI) test and can be used for measuring seronegativity or suceptibility to infection.

Abstract: An improvement in a flow control device for permitting fine adjustment of flow rates of fluid in tubing and maintaining established rates of flow. The device includes a clamping member pivotally mounted adjacent a clamping surface and a tubing-engaging surface on the clamping member which progressively closes the orifice of tubing as the clamping member is rotated in one direction. Coacting means on the clamping member cooperate with actuating means on a force-inducing member mounted for rotational movement on the body of the device. The coacting means is located at a point from the point of pivotal attachment of the clamping member which is at least twice the distance from the pivotal attachment to the tubing-engaging surface. Thus a large movement at the coacting point translates into a small movement of the tubing-engaging surface and results in fine adjustment of flow rates.

Abstract: An injection site is disclosed comprising a self-sealing, puncturable member of resilient material and a housing wherein the puncturable member is compressibly confined by a swage-molded edge on the top of the housing. The injection site may be attached to an injection-receiving receptacle such as a medicament injection device as used in an assembly for intravenous administration.

Abstract: Pesticidal concentrate comprising o-isopropoxyphenyl methylcarbamate and an acidifying agent dissolved in a liquid, water soluble, dispersion medium.

Abstract: A plastic bag containing a platelet concentrate is disclosed wherein the viability of platelets remains at a high level even after storage at 22.degree. C. for up to five days. The bag in which the platelet concentrate is stored comprises walls of polyvinyl chloride film containing tri 2-ethylhexyl trimellitate as plasticizer together with epoxidized vegetable oils for heat stabilization of the film. The film may also contain metal soaps in preferred formulations.

Abstract: Existing and potential prekallikrein activator (PKA) can be removed from an immune serum globulin (ISG) solution using an ion exchange material to remove both existing PKA and a kallikrein-activatable precursor to PKA (Factor XII) found to be present in ISG solutions.

Abstract: An improvement in a flow control device for permitting fine adjustment of flow rates of fluid in tubing and maintaining established rates of flow. The device includes a clamping member pivotally mounted adjacent a clamping surface and a tubing-engaging surface on the clamping member which progressively closes the orifice of tubing as the clamping member is rotated in one direction. Coacting means on the clamping member cooperate with actuating means on a force-inducing member mounted for rotational movement on the body of the device. The coacting means is located at a point from the point of pivotal attachment of the clamping member which is at least twice the distance from the pivotal attachement to the tubing-engaging surface. Thus a large movement at the coacting point translates into a small movement of the tubing-engaging surface and results in fine adjustment of flow rates.