Patents Assigned to Cytonome/ST, LLC
  • Patent number: 10379029
    Abstract: A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region.
    Type: Grant
    Filed: September 18, 2013
    Date of Patent: August 13, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Rudolf Hulspas, Kristopher Scott Buchanan
  • Patent number: 10371621
    Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: August 6, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr., Emanuel Tito Mendes Machado, Blair D. Morad
  • Patent number: 10274414
    Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: April 30, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
  • Patent number: 10221844
    Abstract: A fluid handling system for a particle processing instrument includes a pump, a pulse attenuator, a pressure transducer, and a pump controller. The pump may be configured to supply a pulsed flow of fluid having a first pulse characteristic to the pulse attenuator. The pulse attenuator may have a single, undivided, volume, fluid inlets, fluid outlets, and a pressure sensor port. The pulse attenuator may supply an outlet flow of fluid having a second pulse characteristic different from the first pulse characteristic. The pressure transducer may be in fluid communication with the pressure sensor port and in control communication with the pump controller. The pump controller may be in control communication with the pump to maintain a substantially constant nominal pressure within the pulse attenuator by controlling the pump motor.
    Type: Grant
    Filed: May 15, 2015
    Date of Patent: March 5, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Christopher D. Lofstrom, Coby S. Hughey, Blair D. Morad
  • Patent number: 10222378
    Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: March 5, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
  • Patent number: 10215995
    Abstract: Large area, low f-number optical systems, and microfluidic systems incorporating such optical systems, are disclosed. Large area, low f-number optical systems may be used to collect light from plurality of micro channels associated with a plurality of flow cytometers. The optical systems may be configured to collect light from a source area having an object lateral length or width within a range of 25 mm and 75 mm, configured to have an f-number within a range of 0.9 to 1.2, and configured to have a working distance within a range of 10 mm to 30 mm.
    Type: Grant
    Filed: May 16, 2013
    Date of Patent: February 26, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Donald Francis Perrault, Jr., Johnathan Charles Sharpe
  • Patent number: 10197493
    Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: February 5, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
  • Patent number: 10190960
    Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: January 29, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr.
  • Patent number: 10065188
    Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: September 4, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
  • Patent number: 10029263
    Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.
    Type: Grant
    Filed: March 22, 2013
    Date of Patent: July 24, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Sebastian Böhm, John R. Gilbert, Manish Deshpande
  • Patent number: 10029283
    Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.
    Type: Grant
    Filed: August 17, 2015
    Date of Patent: July 24, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Manish Deshpande, John R. Gilbert
  • Patent number: 10025322
    Abstract: A fluid handling system for supplying a working fluid to a fluid flow instrument is disclosed. The system includes a controller configured to receive sensor signals indicative of a deformation of a flexible barrier located between a control fluid volume containing a control fluid and a working fluid volume containing the working fluid. Based on the sensor signals, the controller may send signals to control the operation of a working fluid flow generator in order to regulate or control the fluid characteristic of the working fluid being provided to the fluid flow instrument.
    Type: Grant
    Filed: May 15, 2015
    Date of Patent: July 17, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Christopher D. Lofstrom, Coby S. Hughey, Blair D. Morad
  • Patent number: 9964968
    Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: May 8, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, Jr., Blair D. Morad
  • Patent number: 9943847
    Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: April 17, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Sebastian Böhm, Manish Deshpande
  • Patent number: 9823252
    Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: November 21, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
  • Patent number: 9802767
    Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: October 31, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Manish Deshpande, Bernard Bunner
  • Patent number: 9752976
    Abstract: An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.
    Type: Grant
    Filed: February 23, 2015
    Date of Patent: September 5, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Edward Sinofsky, Manish Deshpande
  • Patent number: 9618442
    Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: April 11, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
  • Patent number: D791338
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: July 4, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: Michael I. Morkos, Jeffrey T. Samson
  • Patent number: D815754
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: April 17, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Michael I. Morkos, Johnathan Charles Sharpe, Jeffrey T. Samson, James V. Osburn, Martin Joe Costello