Patents Assigned to Cytonome/ST, LLC
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Patent number: 9943847Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.Type: GrantFiled: April 17, 2015Date of Patent: April 17, 2018Assignee: CYTONOME/ST, LLCInventors: John R. Gilbert, Sebastian Böhm, Manish Deshpande
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Patent number: 9823252Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.Type: GrantFiled: February 13, 2014Date of Patent: November 21, 2017Assignee: CYTONOME/ST, LLCInventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
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Patent number: 9802767Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.Type: GrantFiled: September 19, 2016Date of Patent: October 31, 2017Assignee: CYTONOME/ST, LLCInventors: John R. Gilbert, Manish Deshpande, Bernard Bunner
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Patent number: 9752976Abstract: An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.Type: GrantFiled: February 23, 2015Date of Patent: September 5, 2017Assignee: CYTONOME/ST, LLCInventors: John R. Gilbert, Edward Sinofsky, Manish Deshpande
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Patent number: 9618442Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.Type: GrantFiled: June 15, 2015Date of Patent: April 11, 2017Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair D. Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
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Patent number: 9550215Abstract: A method and a system are provided for detecting particles moving through a detection region or regions for facilitating or processing a sample having one or more particles flowing through the detection region. The particle detection system may include an optically detectable pattern associated with a detection region. The optically detectable pattern may be configured to receive a particle optical signal and produce a patterned optical signal. The detection system may further include a detector configured to analyze the patterned optical signal to determine both a particle characteristic based on a property of the particle optical signal and a particle parameter based on a property of the optically detectable pattern.Type: GrantFiled: May 19, 2014Date of Patent: January 24, 2017Assignee: CYTONOME/ST, LLCInventors: Manish Deshpande, John R. Gilbert
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Patent number: 9529203Abstract: Focal plane shift elements and optical systems with focal plane shifting features for illuminating flow-paths in a fluidic processing system are disclosed. An optical system may include a light source providing an incident first light beam. The optical system may include at least one optical element configured to collect and focus the incident first light beam to produce a second light beam having different portions simultaneously focused at two or more different locations along an optical path, with each location corresponding to a different flow-path of the fluidic processing system. The focal plane shift elements and optical systems with focal plane shifting features may be particularly useful in a microfluidic system.Type: GrantFiled: September 17, 2013Date of Patent: December 27, 2016Assignee: CYTONOME/ST, LLCInventor: Donald Francis Perrault, Jr.
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Patent number: 9446912Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.Type: GrantFiled: August 16, 2013Date of Patent: September 20, 2016Assignee: CYTONOME/ST, LLCInventors: John R. Gilbert, Manish Deshpande, Bernard Bunner
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Patent number: 9339850Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: GrantFiled: August 17, 2015Date of Patent: May 17, 2016Assignee: CYTONOME/ST, LLCInventors: Manish Deshpande, John R. Gilbert
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Patent number: 9335247Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.Type: GrantFiled: March 13, 2014Date of Patent: May 10, 2016Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr., Emanuel Tito Mendes Machado, Blair D. Morad
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Patent number: 9260693Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.Type: GrantFiled: October 17, 2014Date of Patent: February 16, 2016Assignee: CYTONOME/ST, LLCInventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
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Patent number: 9255874Abstract: A fluid stream imaging apparatus having either optics for manipulating the aspect ratio or sensing elements configured for manipulating the aspect ratio of an image of the fluid stream. This application may also relate to a system for acquiring images of a portion of a fluid stream at high speeds for image processing to measure and predict droplet delays for individual forming particles.Type: GrantFiled: February 26, 2014Date of Patent: February 9, 2016Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, Jr., Nemanya Sedoglavich
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Patent number: 9109993Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: GrantFiled: June 23, 2010Date of Patent: August 18, 2015Assignee: CYTONOME/ST, LLCInventors: Sebastian Böhm, John R. Gilbert, Manish Deshpande
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Patent number: 9057676Abstract: A microfluidic multiple channel particle analysis system (1) which allows particles (2) from a plurality of particle sources (3) to be independently simultaneously entrained in a corresponding plurality of fluid streams (4) for analysis and sorting into particle subpopulations (5) based upon one or more particle characteristics (6).Type: GrantFiled: February 4, 2011Date of Patent: June 16, 2015Assignee: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair Morad, Rudolf Hulspas, Donald Francis Perrault, Jr.
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Patent number: 9011797Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.Type: GrantFiled: June 19, 2012Date of Patent: April 21, 2015Assignee: Cytonome/ST, LLCInventors: John R. Gilbert, Sebastian Böhm, Manish Deshpande
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Patent number: 8964184Abstract: An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.Type: GrantFiled: December 13, 2010Date of Patent: February 24, 2015Assignee: Cytonome/ST, LLCInventors: John R. Gilbert, Edward Sinofsky, Manish Deshpande
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Publication number: 20140370536Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.Type: ApplicationFiled: March 13, 2014Publication date: December 18, 2014Applicant: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, JR., Emanuel Tito Mendes Machado, Blair D. Morad
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Publication number: 20140343869Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.Type: ApplicationFiled: May 19, 2014Publication date: November 20, 2014Applicant: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
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Patent number: D791338Type: GrantFiled: May 16, 2014Date of Patent: July 4, 2017Assignee: CYTONOME/ST, LLCInventors: Michael I. Morkos, Jeffrey T. Samson
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Patent number: D815754Type: GrantFiled: May 16, 2014Date of Patent: April 17, 2018Assignee: CYTONOME/ST, LLCInventors: Michael I. Morkos, Johnathan Charles Sharpe, Jeffrey T. Samson, James V. Osburn, Martin Joe Costello